Objective Toevaluatetheefficacyofneoadjuvantchemotherapy(NACT)forlocallyadvancedcervicalcancer(LACC) byusinghighresolutionRESOLVE DWIsequence..Methods 34patientswithLACCconfirmedbypathologyunderwentroutine plainandenhancementMRscanandRESOLVE DWIscan withbvalueof0 mm2/s,1000 mm2/sbeforeNACT,afterthefirst chemotherapy(2.5weekslater)andthesecondchemotherapy(5weekslater).TheADCvaluewascalculated.Thetumorvolumewas calculatedusingtumorsegmentationsoftware.Therateoftumorwithdrawalwascalculatedinthemiddle(2.5weeksafterthefirst chemotherapy)andlatestages(2.5weeksafterthesecondchemotherapy),whichwerejudgedaccordingtotheRECIST1.1standard. Results In29patientsofeffectivetreatment,theADCvaluesoftheLACCsignificantlyincreasedfrompre-chemotherapy[(0.887± 0.14)×10-3 mm2/s]toafterthefirstchemotherapy [(0.974±0.12)×10-3 mm2/s]andsecondchemotherapy [(1.023±0.17)× 10-3 mm2/s](t=2.403 ,P<0.05;t=3.397,P<0.05).Theratesoftumorwithdrawalsignificantlyincreasedfromthefirstchemotherapy (58.21%±20%)tothesecondchemotherapy(77.60%±12%)(t=4.297,P<0.05)I.n5casesofineffectivetreatment,theADCvaluesofthe LACCshowedadownwardtrendfrompre-chemotherapy[(0.867±0.06)×10-3 mm2/s]toafterthefirstchemotherapy[(0.848±0.16 )× 10-3 mm2/s]andsecondchemotherapy [(0.842±0.15)×10-3 mm2/s](t=0.934,P=0.378;t=0.886,P=0.401).Theratesof tumorwithdrawalshowedadownwardtrendfromthefirstchemotherapy(14.14%±15%)tothesecondchemotherapy(-21.22%±13%) (t=1.003,P=0.345).Conclusion TheADCvalueofRESOLVEDWIandtherateoftumorwithdrawalmaybeusedtoevaluatethe efficacyofNACTforthepatientswithLACC,whichwouldhavegoodclinicalpracticalvalue.

OBJECTIVE: To study on the expression and clinical significance of interleukin 10 receptor 1(IL-10R1)in T lymphocytes in peripheral blood of children with food allergy. METHODS: The food allergy group included 50 cases of children admitted in Department of Pediatrics of Peking University Third Hospital from July 1,2017 to December 31,2017 and were diagnosed with food allergy. A total of 25 cases of healthy children who visited Child Health and Development Center of Peking University Third Hospital in the same period with no food allergy manifestation were selected as a healthy control group. The clinical manifestations of food allergy in different age groups were compared.Flow cytometry was used to examine the expression of IL-10R1 in CD4~+T cells and CD8~+T cells in peripheral blood and the MFI. According to the results of allergen specific Ig E test,the food allergy children were classified as allergen specific Ig E positive group and negative group. The expression of l L-10R1 in the CD4~+T lymphocytes and CD8~+T lymphocytes in peripheral blood and the MFI of the two groups were compared. A severity index of clinical symptoms and signs of food allergy was used to grade illness severity of food allergy children. Then it analyzed the correlation between the expression rates of IL-10R1 in CD4~+T cells and CD8~+T cells in peripheral blood and the scores. RESULTS: The expression rates and MFI of IL-10R1 in CD4~+T cells in peripheral blood of food allergy group were lower compared to the control group. The median expression rates and MFI were 40.23 versus 45.32,12.18 versus 17.69(Z=-2.506,-5.547,P=0.012,0.000,respectively). The expression rates and MFI of IL-10R1 in CD8~+T cells in the peripheral blood of food allergy group were also lower than the control group. The median expression rates and MFI were 34.50 versus 39.46,12.47 versus 17.28(Z=-4.035,-5.226,P=0.000,0.000,respectively). IL-10R1 expression rates and MFI on CD4~+T cells and CD8~+T cells in peripheral blood of allergen specific Ig E positive group were similar to those of the negative group. The expression rates of IL-10R1 on CD4~+T cells and CD8~+T cells in peripheral blood of children with food allergy had no correlation with the symptom and sign scores of food allergy. CONCLUSION: The decrease of the expression of IL-10R1 in CD4~+T cells and CD8~+T cells in peripheral blood may be associated with food allergy of children;the expression of IL-10R1 may be involved in the pathogenesis of food allergy mediated by Ig E and non-Ig E,but it was not associated with the severity of food allergy.

OBJECTIVE: To evaluate dynamic contrast-enhanced MRI in conjunction with MR subtraction in the differential diagnosis of benign and malignant breast tumors at 3 Tesla. METHODS: A total of 78 patients with breast tumors enrolled in this study, including 45 malignant lesions and 33 benign lesions verified by histopathology. Dynamic MR contrast enhanced imaging was done by T1 high resolution isotropic volume excitation sequence. MR subtraction was used to retrospectively analyze the MR dynamic image. A dynamic phase subtraction (DPS) map is a map image with pixel-by-pixel subtraction of an early-phase image from a delayed maximum enhancement phase image obtained in a dynamic study. The sensitivity and specificity were calculated with or without subtraction in the diagnosis of benign and malignant breast tumors. RESULTS: The sensitivity of benign breast masses increased from 0.879 to 0.939, and the specificity increased from 0.818 to 0.909 with reference to the DPS map. There was statistical difference between with or without DPS (Z=2.023, P=0.043). The sensitivity of breast malignant masses increased from 0.889 to 0.933, and the specificity increased from 0.867 to 0.911 with reference to the DPS map, with statistical difference between with or without DPS map (Z=2.294, P=0.021). The pattern of TIC changed from continuous to a plateau in 8 patients, from a plateau to washout in 10, and from continuous to washout in 5. No changes were observed in the other 55 patients. CONCLUSION MR Subtraction is a simple and useful technique to identify breast lesions. It helps to accurately set the location of the ROI TIC and improve the detection rate of benign and malignant breast tumors.
Breast Neoplasms ; diagnosis ; Contrast Media ; Diagnosis, Differential ; Female ; Humans ; Image Enhancement ; Magnetic Resonance Imaging ; Retrospective Studies ; Sensitivity and Specificity

OBJECTIVE: To explore the diagnostic value of the 3.0T magnetic resonance liver diffusion weighted imaging with background suppression (DWIBS) in liver foci of space occupying lesion. METHODS: A total of 43 cases of liver bureau stove perch pathological change were included: 15 were hepatocellular carcinoma (HCC) with 24 lesions; 7 were liver metastatic tumor with 13 lesions; 10 were liver hemangioma with 12 lesions; and 11 liver cyst with 20 lesions. After taking the conventional T1WI and T2WI sequence, the magnetic resonance background suppression diffusion imaging technology (diffusion weighted imaging with background suppression, DWIBS) was applied, following the dynamic enhanced scan. With the MRI to DWIBS workstation for classifying positron emission computed tomography (PET) processing, the T2WI diagram and dynamic enhanced diagram were compared respectively for the 3 sequences of lesion detection rate, T2WI, and DWIBS, to enhance the delay time between the two joint and combined lesion detection rate. With the MRI workstation software scanning image generation ADC diagram, the ADC values were measured for liver cancer, liver metastatic tumor, liver hemangioma and liver cysts. RESULTS: The 3 sequences of detection rates of the T2WI, DWIBS and enhanced delay period T1WI were 91.3%, 94.2%, and 95.6%. The detection rate of DWIBS plus T2WI was 92.7%; that of T2WI plus enhanced delay time was 94.9%, and that of DWIBS plus enhanced delay time was 96.3%, with the rate of DWIBS plus enhanced delay period obviously higher than that of the DWIBS plus T2WI (P<0.05). The ADC value of the benign liver tumor was obviously higher than that of the malignant tumors: hepatic cyst (2.614 ± 0.57)×10⁻³ mm²/s, liver hemangioma (2.055 ± 0.21)×10⁻³ mm²/s, metastatic carcinoma (1.374 ± 0.32)× 10⁻³ mm²/s, and liver cancer (1.287 ± 0.14)×10⁻³ mm²/s. Except for the liver cancer and the liver metastatic tumor, there was significant difference between the other groups (P<0.05). CONCLUSION Combing the DWIBS technology, the PET-like images and the ADC value acquired, the combined enhanced sequences could further facilitate the demonstration of the liver foci of space occupying lesion, the accuracy of identification and diagnosis of the liver foci of space occupying lesion.
Adult ; Aged ; Carcinoma, Hepatocellular ; diagnosis ; Diagnosis, Differential ; Diffusion Magnetic Resonance Imaging ; methods ; Female ; Hemangioma ; diagnosis ; Humans ; Liver Neoplasms ; diagnosis ; Male ; Middle Aged ; Retrospective Studies ; Young Adult

PURPOSE: Cancer-associated fibroblasts (CAFs) activated by cancer cells has a central role in development and malignant biological behavior in colorectal cancer (CRC). Adult fibroblasts do not express Snail, but Snail-positive fibroblasts are discovered in the stroma of malignant CRC and reported to be the key role to chemoresistance. However, the reciprocal effect of CAFs expressed Snail to chemoresistance on CRC cells and the underlying molecular mechanisms are not fully characterized. MATERIALS AND METHODS: Snail-overexpressed 3T3 stable cell lines were generated by lipidosome and CT26 mixed with 3T3-Snail subcutaneous transplanted CRC models were established by subcutaneous injection. Cell Counting Kit-8, flow cytometry and western blotting assays were performed, and immunohistochemistry staining was studied. The cytokines participated in chemoresistance was validated with reverse transcriptase-polymerase chain reaction and heatmap. RESULTS: Snail-expression fibroblasts are discovered in human and mouse spontaneous CRCs. Overexpression of Snail induces 3T3 fibroblasts transdifferentiation to CAFs. CT26 co-cultured with 3T3-Snail resisted the impairment from 5-fluorouracil and paclitaxel in vitro. The subcutaneous transplanted tumor models included 3T3-Snail cells develop without restrictions even after treating with 5-fluorouracil or paclitaxel. Moreover, these chemoresistant processes may be mediated by CCL1 secreted by Snail-expression fibroblasts via transforming growth factor β/nuclear factor-κB signaling pathways. CONCLUSION: Taken together, Snail-expressing 3T3 fibroblasts display CAFs properties that support 5-fluorouracil and paclitaxel chemoresistance in CRC via participation of CCL1 and suggest that inhibition of the Snail-expression fibroblasts in tumor may be a useful strategy to limit chemoresistance.
Adult ; Animals ; Blotting, Western ; Cell Count ; Cell Line ; Colorectal Neoplasms* ; Cytokines ; Drug Resistance, Multiple ; Fibroblasts* ; Flow Cytometry ; Fluorouracil ; Humans ; Immunohistochemistry ; In Vitro Techniques ; Injections, Subcutaneous ; Mice ; Paclitaxel ; Snails ; Transforming Growth Factors

Objective:To investigate the complex Calculus Bovis-target-keratitis network and to explore the molecular mechanism of Calculus Bovis treating keratitis through network pharmacology. Methods:Genes related to keratitis were searched in the online DisGeNET database and the protein-protein interaction (PPI) network of keratitis-associated proteins was constructed.The components isolated and identified in Calculus Bovis were collected through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP, https: //tcmsp-e.com/tcmsp.php), Chemistry Database by Shanghai Institute of Organic Chemistry of CAS (http: //www.organchem.csdb.cn), and published literature.The canonical SMILES information of the collected components was exported, which were submitted to the SwissTargetPrediction platform to predict potential targets of the components.The active component-predicted target network of Calculus Bovis was constructed and merged with the PPI network of keratitis-associated proteins to build the active component-potential target network of Calculus Bovis and systemically investigate the potential targets and signal pathways of Calculus Bovis in treatment of keratitis.The component-target-pathway network was established to analyze the mechanism of Calculus Bovis treating keratitis. Results:Thirty-nine components isolated and identified in Calculus Bovis were searched and 65 target genes related to keratitis were screened.Of the 28 potential targets involved in Calculus Bovis treating keratitis, there were 7 direct targets, including tumor necrosis factor, caspase 1, Toll-like receptor 9, C-X-C motif chemokine ligand 8, interleukin-6, mitogen-activated protein kinase 8, neurotrophic receptor tyrosine kinase 1.The 28 potential targets were annotated to 12 entries for biological process, 18 for cellular components and 13 for molecular function.In the Kyoto encyclopedia of genes and genomes pathway enrichment analysis, 10 signal pathways were identified as enriched categories, which were mainly related to human cytomegalovirus infection, amoebiasis, antifolate resistance, PI3K-Akt signaling pathway, rheumatoid arthritis, apoptosis, cytokine-cytokine receptor interaction, malaria, non-alcoholic fatty liver disease, interleukin-17 signaling pathway. Conclusions:Calculus Bovis may play an adjuvant therapeutic effect on keratitis through anti-inflammatory, antibacterial, antiviral, immune regulation, inflammatory regulation and other functions.