Abstract The genus Saxifraga, one of the largest genera of the Saxifragaceae family, comprises 540 species and is distributed widely in mountainous and rocky regions, with 12 species being found in Mongolia. Saxifraga spinulosa Adams is a perennial herbaceous plant common in mainland China, Russia, and Mongolia, inhabiting stony marginal terrain. The isolated compounds from Saxifraga spinulosa were screened for DPPH radical-scavenging activity, with Trolox as a positive control (IC₅₀ 23.3 µM). All the new glucosides exhibited potent activities (IC₅₀ 19.0–72.9 µM). A crude ex- tract of S. spinulosa has been reported to display the highest DPPH radical-scavenging activity among numerous Mongolian medicinal plants, which may now be attributed, at least in part, to the presence of the new flavonoid and galloyl group-containing isolated compounds.