Introduction: Studies on the development and validation of semi-quantitative food frequency questionnaires (SQ-FFQ) for assessing omega-3 (Ω-3) long-chain polyunsaturated fatty acids (LC-PUFAs) intake by pregnant women are few. This study aimed to determine the validity of a newly developed SQ-FFQ for assessing the LC-PUFA intake among Indonesian urban pregnant women. Methods: A cross- sectional study was carried out in 2015 on 100 Indonesian pregnant women who were in their late 3rd trimester, living in the urban setting of Jakarta. As a test tool, the SQ-FFQ was administered before the trained nutritionists executed the reference tool of non-consecutive two-day 24-hour dietary recalls (2DRs). The nutrients of interest were a total of Ω-3, eicosapentanoic acid (EPA), docosahexaenoic acid (DHA), alpha-linolenic acid (ALA), total Ω-6, linoleic acid (LA), arachidonic acid (AA), and LC-PUFAs. Statistical correlation, cross-classification and the Bland-Altman plot analysis were done to determine the agreement between tools. Results: Energy-adjusted correlation coefficients between SQ-FFQ and 2DRs were 0.385, 0.349, 0.352, 0.380, 0.338, 0.408, 0.409, 0.331, 0.341 and 0.341 for fat, total Ω-3, ALA, EPA, DHA, total Ω-6, LA, AA and LC-PUFAs, respectively and were statistically significant (p<0.05). Misclassification of these nutrients from SQ-FFQ and 2DRs was <6%. The Bland-Altman plots showed most of the points fell within the 95% limits of acceptable agreement for DHA, EPA, and LA. Conclusion: The newly developed SQ-FFQ of this study is a valid instrument for assessing of Ω-3 LC-PUFAs intake among Indonesian pregnant women living in urban area. Its further validation with relevant biomarkers is recommended.

Introduction: Skipping breakfast may contribute to poor academic and nutritional status among school children. The current study aimed to determine the effect of the ‘MAPAGI’ (Makan Pagi Bergizi) interactive video game on elementary student’s breakfast knowledge and attitude. Methods: This was a quasi-experimental equivalent group study with a pre-post test control group design. In total, 228 students aged 9-11 years were drawn by proportional stratified random sampling from two urban schools and were distributed equally into intervention (IG) and control groups (CG). Both groups were administered with similar printed media for 10-15 minutes. Video game was delivered only to IG for two consecutive days, 30 minutes each day. Pre-test (PT0) was performed a day before leaflet administration, while post-test was performed twice – after leaflet administration (post-test 1/ PT1) and a week after the last video game (post-test 2/PT2). Paired, independent t-test and analysis of covariance (ANCOVA) were employed to answer the research questions. Results: The mean changes (PT1-PT0 and PT2-PT0) in knowledge score for IG (4.82±2.53 and 3.25±2.47) significantly differed compared to CG (1.75±2.66 and 1.25±2.69). The mean changes in attitude score for IG (7.16±7.17 and 7.34±7.11) also significantly differed compared to CG (2.83±6.58 and 2.64±6.90). After adjustment for potential confounding factors, there was still a significantly greater score in children’s knowledge and attitude after a week’s administration of MAPAGI video game in IG. Conclusion: ‘MAPAGI’ improved school children’s knowledge and attitude, which may lead to the good behaviour of having breakfast.