Humans ; Medicine, Chinese Traditional ; Psoriasis ; classification ; diagnosis ; therapy ; Syndrome

Objective To investigate the medium and long-term influence of silicon oil versus heavy silicone oil on rabbit retinas.Methods 28 health standard rabbits were randomly divided into A,B and C groups,with 12,12 and 4 rabbits respectively.All rabbits received routine vitrectomy and tamponade with silicone oil (group A),or heavy silicone oil (group B) or balanced salt solution (group C).After 4,8,12 and 24 weeks,the retinal b-wave amplitude was measured by ERG,posterior retinal thickness was measured by optical coherence tomography (OCT).Retinal ultrastructure and tissue morphology were observed by transmission electron microscopy and optical microscopy.Results Compare to group C,the b-wave amplitude decreased at 4 weeks after surgery,and decreased at 8 weeks after surgery for group B,and decreased at 8 weeks after surgery,and decreased at 24 weeks after surgery for group A.The decreases were greater in group B than group A at 8,12,24 weeks after surgery,the difference was statistically significant (P＜0.05).The posterior retinal thickness of group A and B was thinner than group C at 24 weeks after surgery (P＜0.05).The decreases were greater in group B than group A,the difference was statistically significant (P＜0.05).Transmission electron microscopy and optical microscopy revealed severe pathological changes of retinal ultrastructure and morphology in group A and B rabbit eyes,at 12 weeks and 8 weeks after surgery respectively.The changes were more severe in group B and group A,including edema and necrosis in cone/rod cells,in disk membranes,mitochondria,cytoplasm,nucleus and other organelles.The morphological changes were also more severe in group B and group A,including degenerations of ganglion cell layer,inner nuclear layer changes.Those changes became more severe when the tamponade time extended.Conclusion The heavy silicone influence on visual function,ultrastructures,histomorphology of rabbit retinas is much worse than the silicon oil,and the effect is more significant with its time prolong.

Objective To study the effect of static magnetic fields on the micronucleus in polychromatic erythrocytes(PCE) in the bone marrow and and the lipid peroxidation in the brain tissue in mice.Methods Fourty Kunming mice were randomly divided in to the negative control group,positive control group(cyclophosphamide:145 mg/kg) and the static magnetic fields(20,40,50,60,80 and 100 mT) exposure group,exposed twice a day,for two hours each time.After 15 days,the micronuclei rate of the bone marrow,blood cells and the activity of superoxide dismutase(SOD),the protein malonaldehyde(MDA) were determined.Results Compared with the control,the micronuclei rate in 50,60,80 and 100 mT exposure group increased significantly,the amount of leukocyte significantly decreased,the activity of SOD in the brain tissue in 60,80 and 100 mT exposure group decreased and the content of MDA increased significantly.Conclusion The present experiment demonstrats that static magnetic fields exposure at doses of 50,60,80 and 100 mT may induce the micronuclei rate increase,the amount of leukocyte decrease,the activity of SOD in the brain tissue decrease and the content of MDA increase.

Objective To identify the characteristics of Candida utilis uricase and that of Bacillus fastidious intra-cellular uricase.Methods The new strains were cultivated to prepare the uricase.Then the uricase was purified by ammonium sulfate fractionation precipitation,DEAE-cellulose 52 chromatography and preparative PAGE.The activity,characterization and subunit constitution were determined with the purified uricase.Results The two uricas-es were the iso-tetramer.The single peptide chain molecular weight and total molecular weight of Candida utilis uricase were 33.0 ku and 134.0 ku individually,the optimum pH was close to 8.8 and more than 50% activity was reserved at pH 7.4,the Michaelis-Menten constant was (32.8 ± 3.1) μmol/L (n = 10) and the inhibition constant of xanthine was (4.8 ± 0.2) μmol/L(n = 3) for this uricase.For Bacillus fastidious intracellular uricase,its single peptide chain molecular weight and total molecular weight were 35.7 ku and 151.0 ku,the optimum pH was over 9.0 and less than 30% activity left at pH 7.4,the Michaelis-Menten constant and the inhibition constant of xanthine were (204 ± 14) μmol/L(n = 8) and (41 ± 7) μmol/L(n = 5) individually for it.Conclusion Through the research,a significant theoretical basis was funded for constructing hybrid medicinal uricase to treat diseases as-sociated with hyperuricemia.

Objective To study the effect of CO 2 pneumoperitoneum and enteric disturbance on serum ?-endorphin (?-EP) in SD rats, in order to investigate their influence on peri-operative stress responses in SD rats. Methods A total of 120 SPF-grade male SD rats were randomly divided into four groups with 30 rats in each group. The four groups received CO 2 pneumoperitoneum (Group A), a 5 cm abdominal incision (Group B), a 5 cm abdominal incision with gastroenteric disturbance (Group C), and intraperitoneal anesthesia (Group D or Control Group), respectively. Concentrations of serum ?-EP of these groups were measured 10 min, 20 min, and 40 min after the beginning of surgery, respectively. Results Concentrations of serum ?-EP in the Group A 10 min, 20 min, and 40 min after the beginning of surgery were 274 7?66 6 pg/dl, 157 3?63 8 pg/dl, and 163 9?74 5 pg/dl, respectively, which were all extremely significantly higher than those in the Control Group ( P

Objective To explore the diagnostic value of secretory phospholipase A2 (sPLA2) and lysophosphatic acid (LPA) for ovarian cancer and evaluate the correlation of sPLA2 activity and LPA level to the prognosis in the patients with ovarian cancer.Methods ~3H oleic acid labeled E. coli membrane and spectrophotometry were used to detect the original and recurrent ovarian cancer. The motive observation between sPLA2 activity and LPA level was done.At the same time, The sensitivity and specificity for the diagnosis of ovarian cancer were analyzed.Results Comparing the original ovarian cancer group with the ovarian benign tumor group, the difference between sPLA2 activity (24.47 ?5.64 U/L,4.94 ?1.86 U/L) and LPA levelc(6.35 ?2.32 ?mol/L,1.92 ?0.75 ?mol/L) were significantly (P

China ; Developed Countries ; Insurance, Health ; Occupational Health

Adult ; Farmer's Lung ; Female ; Humans

Abstract: A new selaginellin derivative named as selaginellin S (1) was isolated from the whole plants of Selaginella pulvinata (Hook. et Grev.) Maxim. (Selaginellaceae), together with a known one (selaginellin M, 2). Compounds 1 and 2 were separated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were determined on the basis of extensive spectroscopic analysis including IR, MS, 1D and 2D NMR experiments, as well as ECD calculations. Compound 1 is a key intermidiant in the biosynthesis pathway of selaginellins. Compound 2 is first reported in this plant.

BACKGROUND:Rehabilitation outcomes of patients with spinal cord injury are associated with degree of injury, therapeutic method, recovery time and subsequent treatment. Multidisciplinary, comprehensive, specialized rehabilitation unit can provide a better recovery after spinal cord injury. OBJECTIVE:To review the effect or combined effect of rehabilitation units after spinal cord injury. METHODS:A computer-based search of Springer and PubMed databases was done using the keywords of“spinal cord injury, rehabilitation practice, outcomes”, which appeared in the title and abstract. Final y, 44 English papers were included in result analysis. RESULTS AND CONCLUSION:Based on practical evidence, various rehabilitation practices are recognized, and then, the relevant information is connected with the outcomes to evaluate the efficacy of rehabilitation interventions. Studies have shown that age had no influence on rehabilitation outcomes in traumatic and non-traumatic mixed samples, and there is also little difference in the rehabilitation outcomes between male and female. Incidence of complications is lowest in the vast majority of patients with spinal cord injury who are initial y admitted to a specialist center of spinal cord injury. The hospital stay can be shorten in patients who can be admitted to a multidisciplinary, comprehensive, specialized division of spinal cord injury as early as possible. Patients who can receive regular, comprehensive outpatient fol ow-up show no significant differences in health perception, independence and depression, but the frequency and degree of certain secondary situations can be significantly reduced.