Objective: To study the chemical constituents in the roots of Aconitum bulleyanum Diels. Methods: The air-dried roots of A. bulleyanum were powdered and extracted by methanol with percolation. After removing the solvent under reduced pressure, the crude extract was dissolved in 1. 5％ HCl solution, and then basified to pH 9 by NaOH (5％) and extracted by ethyl acetate to ob-tain crude alkaloidal extract after ethyl acetate removal. The alkaloidal extract was isolated and purified by column chromatography, and their structures were identified based on spectral analysis ( 1 H-NMR, 13 C-NMR, MS) . Results:Totally 12 diterpenoid alkaloids were isolated from A. bulleyanum and characterized as foresaconitine (1), crassicauline A (2), chasmanine (3), talatisamine (4), 14-debenzoyl-franchetine (5), pengshenine A (6), crassicautine (7), yunaconitine (8), franchetine (9), liljestrandisine (10), transconitine B (11) and pseudoaconine (12). Conclusion:Compounds 3-7, 10-12 are isolated from this plant for the first time.

Objective To study the incidence of complications of visceral and vascular injury in laparoscopic surgery for gynecologic diseases,and to discuss the ways to decrease the incidence. Methods The data of 2684 patients who received laparoscopic surgery from Januray 2003 to December 2005 in the Department of Obstetrics and Gynecology were reviewed retrospectively.The incidence and treatment of complications of visceral and vascular injury were observed. Results The total incidence rate of complications was 2.53%(n=68),and that of the visceral and vascular injury was 0.37%(n=10).Four cases of injury were related with trocar punctures(injury of omental blood vessel,n=2;postperitoneal vessel,n=2),three took place during the operation (severe bleeding,n=1;bladder injury,n=2),and the other three were observed in the postoperative stage(ureter injury,n=2;intestinal fistula,n=1). Conclusion The complications in laparoscopic surgery for gynecologic diseases are increased with the extension and difficulty of operation,and are closely related with the experience of the surgeons.Proper candidates for the surgery and established operative technique are the key factors in decreasing the incidence.

The methods of separation, enrichment, and analysis of trace mercury in water samples in recent years were reviewed in the present paper. Many methods can be used to analyze trace mercury after separation and enrichment. Spectrophotometry is cheap and simple. Atomic fluorescence spectrometric method is accurate in quantitative analysis. The chromatography method is usually used in the morphological analysis of mercury. Multistep analysis of mercury can be done by joint methods in one time.

Chronic active Epstein - Barr virus infection(CAEBV) is an uncommon outcome of EBV infection and may present as severe of fulminant syndrome with high- mortality. It is characterized by chronic or recurrent infectious mononucleosis-like symptoms persisting over a long time and by an unusual pattern of anti-EBV antibodies. Although it occurs in immunocompetent individuals, a number of subtle immunologic defects have been reported in patients with CAEBV. Up to now, there are still no diagnostic criteria of CAEBV in China,so the author introduce it with respect to its diagnosis,history,pathogenesis and therapeutic approaches.

AIM: To study the pharmacokinetics of magnesium isoglycyrrhizinate (MgIG),which was diluted by 5%glucose injection in a total volume of 250 mL, after a single and multiple intravenous dose in 10 healthy volunteers.　METHODS: MgIG 100 mg once daily for 9 d in the multiple-dose regiment. Plasma MgIG concentrations are measured using high performance liquid chromatography (HPLC). Waters HPLC instrument was used with the Hypersil ODS2 C18 (5 μm, 300 mm×4.6 mm) column. The mobile phase was composed of 0.23 mol·L-1 phosphate buffer (pH=7.4):acetonitrile (79∶21).Flow rate was 1.0 mL·min-1 and column temperature was maintained at 40°C. The UV detector was set at 250 nm. The concentration time curves of MgIG were fitted to a two-compartment open model.　RESULTS: The pharmacokinetic parameters obtained from the single-dose study were as follows: cmax=(29±s 4) mg·L-1;t(1)/(2)α=(1.72±0.27) h;t(1)/(2)α=(23±3) h and AUC0-72=(448±75) mg·h·L-1. The steady-state pharmacokinetic parameters were: cssmin=(13±3) mg·L-1;cssmax=(42±6) mg·L-1;cav=(21±4) mg·L-1;t(1)/(2)α=(1.6±0.4) h;t(1)/(2)β=(24±4) h and AUCss0-24=(513±108) mg·h·L-1.CONCLUSION:The distribution and elimination rate of MgIG were not changed after multiple intravenous administration.

[Objective]Discussion on TCM name and pathogenesis of hypertension. [Method]To further explore and analyze the TCM name, location as wel as pathogenssis of hypertension after learning“Neijing”. [Result]Hypertension corresponding TCM diseases should be the veins bulge from“Neijing”. The location of the disease is heart,while it relates to internal organs. The main pathogenesis is that heart commanding blood abnormality. [Conclusion ]The traditional classical dialectical mode from the liver treatment of hypertension should be changed,the clinical treatment of hypertension is due to the heart according to the theory of pathogenesis which the heart commands blood vessels.Seeking the key reason is important to differential treatment of hypertension.

To compare the advantages and the disadvantages of the teaching of problem-based learning(PBL) with lecture based learing(LBL) in Pathophysiology with the questionnaire.

In medical education,to cultivate the medical students with high comprehensive quality,we have carried out the teaching reform in Pathophysiology education.

Objective To evaluate DSA feature, the value, efficacy and side effect, survival time of microcathereter superselective catherization intervenetional treatment on liver metastases cancer. Methods 60 cases with liver metastases cancers were cannulated through common femoral artery by seldinger technique. If the artery was lack, second radiography was given through superselective hepatic artery. On the base of DSA feature of liver metastases, all the patients were divided into two groups: superselective- catherization group and nonsuperselective-catherization group. There were 30 patients in each group respectively. Microcathereter was used in the former to "embed" the tumor vessel and "siphonal" technique was used in the later. Compared the changes of the two groups in volume, the deposit of iodized oil in liver matastases after treating one month and the changes of ALT, ALB, TBIL in blood. The efficacy and side effect were compared in two groups. The survival time and DSA feature of using microcathereter were also analyzed. Results The DSA feature and efficacy of superselective-catherization group was better than that of non-superselective-catherization group, the side effect was mild and the survival time was longer than the former. Conclusion Microcathereter superselective catherization to treat liver metastases cancer has better efficacy, artery radiography, lighter side effect and can prolong the survival time.

The aim of this study was to investigate if there are abnormal fibroblasts derived from the skin surrounding keloids in order to have a better understanding for keloid progression. All the samples were used for cell culture. Flow cytometry was used to compare the apoptotic rate of fibroblasts derived from keloid and its surrounding skin, when it was cultured in serum-deprived medium for 24 hours or was induced by Fas antibody. After cultured in serum-deprived medium for 24 hours, the apoptotic rate of fibroblasts derived from the surrounding skin of keloid increased to an amount between that of normal skin and keloids. The apoptotic rate of normal skin fibroblasts increased more than that of keloids. Moreover, when induced by Fas antibody, the apoptotic rate of fibroblasts derived from the surrounding skin increased not so high as that of normal skin(P0. 05). Therefore, at least there are some fibroblasts in the surrounding skin of keloids, in which apoptosis can not be induced as in normal skin.