Objective To evaluate the clinical efficacy of neo-adjuvant chemotherapy in the treatment of cervical cancer. Methods A total of 32 patients,of which 12 cases of Ⅰ b2,8 cases of Ⅱ a,5 case of Ⅱ b and 7 cases of Ⅲ, were all treated with 2 - 3 cycles of PF chemotherapy before treatment with cisplatin 100 mg/m2, d 1;5-fluorouracil 1000 mg/m2 ,d1-5. The chemotherapy was performed for 3 weeks followed by three weeks rest as one cycle. Results Short-term effect was 81.3% (26/32), with 3 cases of Ⅰ b2 and 1 cases of Ⅱ a had been completely relieved after 2 - 3 cycles of chemotherapies. Simultaneously, chemotherapy improved the removal rate of surgery. Among these 32 patients, there were 4 cases achieved complete response (CR) ,22 cases achieved partial response (PR) ,5 case of stable disease ( SD), 1 cases of progressive disease(PD). The overall response rate( CR +PR) was 81.3% (26/32) ,and the control rate ( CR + PR + SD) was 96. 9% (31/32). The main adverse events included myelosuppression and gastrointestinal response, which were all tolerable. Conclusions The neo-adjuvant chemotherapy reduced the size of cancer,which can improve removal rate of surgery and the quality of life.

Based on the translational research measurement and backtracking model put forward in this paper,it takes the new drug approved to enter into the market by American FDA from 2006-2015 as the fundamental data to measure the knowledge relations among new drug products-core patent-scientific paper-fund assistance,and puts forward the policies and suggestions for promoting innovation of pharmaceutical products in China.

Sulfur dioxide (SO_2) is an ordinary air pollutant globally and harm to human health. L-cysteine is the major sulfur-containing amino acid and its normal metabolism can produce hydrogen sulfur (H_2S) and SO_2. It is realized that H_2S has various bioactivities and is the third gasotransmitter after nitric oxide (NO) and carbon monoxide (CO). Recently, attention has been paid to the physiologic effects of endogenous SO_2 and its derivatives (bisulfite and sulfite) in vivo, and recognized that SO_2 and its derivatives can lower blood pressure, change heart rates, participate in inflammatory reactions, and so on, suggesting that endogenous SO_2 may modulate the physiologic functions in vivo as a bioactive molecule.

Genetic Engineering ; Humans ; Rabies ; virology ; Rabies virus ; genetics ; pathogenicity ; Viral Proteins ; genetics ; Virulence

AIM:To research the effect of safflower in renal intertistial fibrosis by GFR and renal biopsypathology with Sprague-Dnwley(SD) rat model.METHODS:All rats were distributed into 6 groups.Group A was normal control,group B received sham operation,group C was vehicle control,group D received vehicle,group E received safflower(10(g?kg~(-1)) body(wt?d~(-1))),and group F received enalapril((12.5)(mg?kg~(-1)) body(wt?d~(-1))).Rats underwent reversible left renal vein obstruction for 6 d,after which the obstruction was relieved.Renal function was determined by measuring GFR with inulin clearances.Both renal tubular epithelial cells(RTECs) and fibronection deposition for renal interstitial fibrosis were identified by immunohistochemistry.The positive and negative areas of per slice was determined by a motic medical 6.0 graphical analytical system.Atrophic tubules were determined by measuring the tubule diameter.10 fields per slice were analyzed at ?400 magnification.RESULTS:Return of glomerular filtration rate(GFR) was seen in group E and goup F,which was significantly better than group D(P

Aim To investigate the effect of sulfur dioxide (SO2) on blood pressure and aortic structure of the spontaneous hypertensive rat(SHR).Methods Sixteen male SHRs at the age of 4 weeks were randomly divided into SHR control group and SHR+Na2SO3/NaHSO3 group,respectively.Another eight male WKY rats served as WKY control group.Five weeks later, the blood pressure was determined.The rat aortas were dyed with Hart's Method. The morphometric parameters including outer radius, lumen radius and the wall thickness were calculated by Leica workstation. The proliferative index (PI) of smooth muscle cells was detected by immunohistochemical assay.Results The blood pressure of SHR control increased compared with that of WKY control rats(P

Objective To investigate the function and mechanism of embryonic stem cells against Lewis non-small cell lung cancer in vivo. Methods Based on the mouse Lewis non-small cell lung cancer model, we have tested some tumor growth indexes and investigated the immune response of embryonic stem cells against cancer cells. Results Compared with the mice in control group, mice in experimental group received obvious antitumor immunity, which means more activated lymphocytes and antitumor cytokines, resulted in the effective control and inhibition of tumor development. Conclusion Besides the antitumor effect in vitro, embryonic stem cells can also generates immune response in vivo, which could effectively inhibit and/or delay the development of cancer.

Objective To investigate the catalysis of Co - Cu /SiO2 in oxidation of sodium percarbonate (SPC ) for o-nitrochlorobenzene. Methods The solution of o-nitrochlorobenzene was prepared, acetic acid was used to adjust acidity, sodium percarbonate was applied as the oxidant, the self-prepared Co-Cu/SiO2 was used as the catalyzer to catalyze the degradation reaction of o-nitrochlorobenzene and the effect of reaction condition on degradation was researched. Results In the condition of loading amount of metal was 5%, nCo:nCu= 4:1,nCo-Cu=1.90?10-3mol,nSPC=6.36?10-3 mol,cOCNB=50 mg/L and t=80 ℃,pH=4,the degradation efficiency of o-nitrochlorobenzen could reach 92% . Conclusion Co-Cu/SiO2 is efficient in catalyzing the oxidative degradation of sodium percarbonate for o-nitrochlorobenzene and is applicable to the waste water treatment for eliminating o-nitrochlorobenzene

AIM: To explore the possible impact of hydrogen sulfide(H2S) donor-sodium hydrosulfide(NaHS) on endothelin-1(ET-1) and connective tissue growth factor(CTGF) expressions in rats with pulmonary hypertension induced by high pulmonary blood flow.METHODS: Thirty-two male SD rats were randomly divided into 4 groups: shunt group,shunt+NaHS group,sham group and sham+NaHS group.Rats in shunt group and shunt+NaHS group were subjected to an abdominal aorta-inferior vena cava shunt to create an animal model of high pulmonary flow.After 11 weeks of experiment,rat systolic pulmonary artery pressure(SPAP),lung tissue H2S,plasma ET-1 concentration and lung tissue ET-1mRNA expression,as well as pulmonary artery CTGF protein expression were detected.RESULTS: After 11 weeks of experiment,SPAP,lung tissue ET-1mRNA,plasma ET-1 as well as pulmonary artery CTGF expressions were increased markedly,respectively,whereas H2S in lung tissue decreased significantly in rats of shunt group as compared with that in sham group(all P

BACKGROUND: If it is feasible to realize the controllable urination by establishing artificial abdomen-bladder reflex arc basing on remained somatic-reflection below the paralyzed level.OBJECTIVE:To establish an artificial abdomen-bladder reflex arc in canines abdomen to resume the bladder dysfunction due to spinal cord injury.DESIGN:Bilateral comparative observing study with experimental animal as subjects.SETTING:At the Research Institute of Surgery,Third Military Medical University of Chinese PLA.MATERIALS:This study was carried out at the Research Institute of Surgery of Daping Hospital,Third Military Medical University of Chinese PLA from April 2002 to December 2004.Six adult male canines were adopted with body mass of 10-12 Kg.INTERVENTIONS:This is achieved by intradural microanastomosis of the proximal end of right T12 ventral root and the distal end of right S2 ventral root,as well as the distal end of right S2 dorsal root and Ti2 dosal root by end-to-side microanastomosis. After axonal regeneration,the abdomenspinal cord center-bladder new artificial bladder reflex pathway was established,hereby inducing urinating by right abdominal stimulation. The bladder func-tion was assessed with electrophysiological examination and detrusor elec-tromyograms at postoperative 9 months,which was compared with the left controls.MIAN OUTCOME MEASURES: l①Electrophysiological examination.②Bladder contracting function.RESULTS:Data from six canines were analyzed statistically without loss.①Evoked potentials from the anastomosized distal site could be detected by the stimulation (115 mV,1.0 ms)at the right T12 dorsal root in all six canines before and after the spinal cord transaction at the T12 segment level,which was similar to the left side in shape and amplitude.② After paraplegia for 48 hours ,bladder contraction was very quickly initiated by a series of stimuli(1 000 mV, 10 Hz, 2 s) at the right T12 dorsal root with the new reflex arc and the evoked bladder pressure increased to 61% of control side,and to 51% of control side by stimulation at the right abdomen.CONCLUSION:The new bladder artificial autonomic reflex arc is established basing on the somatic reflex at the level higher than the injured con-secutive spinal cord and provides afferent passages for the transduction of bladder sensory,showing satisfactory function of conducting motor excitation. Reconstruction of the bladder autonomic reflex arc by intradural microanastomosis results in bladder contraction,and contributes to controllable urination of canines.