A single intraperitoneal injection (ip) of Astragalus polysaccharide (APS), polysaccharide of Acanthopanax senticosus (PAS) and Lychtm barbamm polysaccharide (LBP) at a dose of up to 10 mg/kg body weight could render splenocytes of adult C57BL/6 mice proliferate significantly higher than normal saline (NS)control. Splenocytes of LBP treated aged mice were 4 times higher than those of NS control of aged mice. The splenocytes of the mice treated with APS, PAS, LBP and NS were incubated with 125 - 1 000 U/ml rIL-2 for 4d in vitro, respectively. The cytotoxicity of LAK cells from the splenocytes of APS, PAS and LBP treated adult mice, in 18 h [125I]UdR release assay, were 70% ~ 120%, 20% -90%, 26% ~ 80% higher than that of the NS control, respectively. The dose of rIL-2 was reduced 75%, 50%, 50%, respectively, in vitro. The cytolytic activities of LAK cells from the splenocytes of LBP treated aged mice were 120%-200% higher than those of the NS control, and the dosage of rIL-2 was decreased more than 75% in vitro.

Adult Kunming mice were immunized with K562 and P815 by a single intraperitoneai injection at a dose of 5?104 cells/murine/d. Anti-P815 and anti-K562 antisera were prepared. Contents of anti-tumor antibodies in the antisera were proved to be high by means of ELISA assay. Splenocytes of C57BL/6 mice and peripheral blood lymphocytes (PBL) from cancer patients were incubated with 125-1000 U/ml IL-2 for 4d. Anti-P815 activity of the LAK cells from C57BL/6 splenocytes was greatly enhanced in the presence of anti-P815 anti-serum at the early stage of culture of LAK and P815 together in 18h [125I]UdR release assay. Anti-K562 activity of LAK cells from PBL was also greatly enlianced by anti-K562 antiserum when the antiserum was added at the early stage of culture. Anti-tumor antibodies in the antisera, not complement and other cytokines, were responsible for the augmentation of NK and LAK cell activities.

In order to observe clinical therapeutic effects of different acupuncture and moxibustion methods on acute lumbago, the patients of acute lumbago were randomly divided into four groups: group A, filiform needle treatment group; group B, filiform needle plus cupping group; group C, filiform needle plus blood - letting puncture and cupping group; group D, filiform needle plus blood - letting puncture, cupping and moxibustion group. All the patients were treated respectively 5 and 10 times. Results showed that there were significant differences in the effective rate and cured rate as the group C and D compared with group A and B respectively (P

Effects of Lycium barbarian polysaccharide (LBP), Astragalus polysaccharide (APS), polysaccharide of Acanthopanax senticosus (PAS) and polysaccharide of lipopolysaccharide (PS) on cytotoxicities of LAK cells from splenocytes of C57BL/6 mices were observed with 18 h 125IUdR release assay. Our study demonstrated that the polysaccharides alone were shown to induce no cytotoxicities. When combined with rIL-2, all of 4 kinds of the polysaccharides augrnented LAK activities in dose-dependent manner. The augmentations reached the maximum at 0.01-0.1 mg ? ml-1 LBP, 0.01 mg ? ml-1 APS and PAS, 0.01 mg ? ml-1 PS. They were found to increase LAK activities in short range of rIL-2 concentrations (250-1000U? ml-1). If the polysaccharides were used beyond the suitable concentrations, they were shown to inhibit LAK cell activities.

Study on protein expression and antigenicity detection of hepatitis C Virus envelope protein E2 in prokaryotic and eucaryotic expression systems.The gene that encoding.HCV E2 protein was cloned in pQE30 and pEF1/HisC.After the expression of E2 protein in E.Coli M15 and COS 7 cell,the expressed proteins were used to detect their antigenicity with ELISA and WB.The results showed that protein E2 was expressed in both prokaryotic and eucaryotic cells.Special reaction could be detected using the expressed proteins and sera from HCV infected people.The studied E2 gene could express the desired proteins in both prokaryotic and eucaryotic expression systems,and glycosylation of the E2 protein happened in COS 7 cell.

Objective To investigate the effects of various concentrations of lidocaine on the N-methyl-D-aspartate ( NMDA)-mediated calcium currents in cultured rat hippocampal neurons. Methods Hippocampal neurons were obtained from newborn Wistar rats (0-24 h after birth) . The hippocampal neurons cultured for 12-14 days were divided into control group and 5 lidocaine groups in which lidocaine was added to the extracellular solution achieving the final concentrations of 10-3 , 10-4 , 10-3 , 10-2 and 10-1 . The neuronal cells were voltage clamped at - 80 mv. The currents evoked by NMDA 100 ?mol?L-1 were recorded. The NMDA-mediated Ca currents were isolated by blocking Na-currents with TTX, K-currents with CsCL and TEACL and non-NMDA receptor (to a large degree AMPA receptor) with CNQX. Current density was calculated (pA / pF) .Results Lidocaine significantly reduced the density of NMDA-mediated calcium currents at concentrations of 10-3- 10-1 ?mol?L-1 compared with that in the control group (P

Objective To determine the risk factors for coronary artery lesions (CALs) in patients with Kawasaki disease (KD).Methods The clinical records of 2331 patients with KD from January 2005 to December 2014 in Beijing Children's Hospital,Capital Medical University were retrospectively analyzed.The relationship between the following factors and CALs was analyzed by univariate and multivariable Logistic regression analysis:age,gender,incomplete KD,total fever duration,intravenous immunoglobulin(IVIG) treatment resistance,white blood cell count,C-reactive protein (CRP),platelet count,sodium and albumin.Results The incidence of CALs was 36.0％(840/2331 cases).Univariate Logistic regression analysis indicated that male patients,incomplete KD,total fever duration ≥10days,IVIG treatment resistance,CRP＞100mg/L,platelet count＞300×109/L and albumin＜35 g/L were associated with CALs (all P＜0.05).Multivariable Logistic regression analysis identified that male patients (OR=1.698,95％ CI 1.383-2.084,P＜0.001),incomplete KD (OR=2.730,95％ CI 2.121-3.515,P＜0.001),total fever duration ≥10 days (OR=2.556,95％ CI 1.975-3.307,P＜0.001),CRP＞100 mg/L (OR=1.556,95％ CI 1.274-1.900,P＜0.001) and albumin＜35 g/L (OR=1.665,95％ CI 1.323-2.096,P＜0.001) were the independent risk factors for CALs.Conclusions The main damage in patients with KD is CALs.The male children with KD,incomplete KD,total fever duration ≥10 days,CRP＞100 mg/L and albumin＜35 g/L were prone to CALs.

Objective To observe the preventive effects of 2.5% sodium bicarbonate solution on the patients who had upper respiratory tract infection caused by fungal with hormone treatment.Methods 36 patients of systemic lupus erythematosus,polymyositis/dermatomyositis were randomly divided into two groups:observer group with glucocorticoid treatment and using 2.5% sodium bicarbonate solution to clean the mouth,and control group only with glucocorticoid treatment.After 7 days,we observed the rate of fungal infection of the two groups.Results There was significant diffience on the rate of fungal infection between observer group and control group(P0.05).Conclusion Using 2.5% sodium bicarbonate solution to clean the mouth can effectively prevent the patients who has upper respiratory tract infection caused by fungal with hormone treatment and decrease the rate of fungal infection.

Murine tumor necrosis factor(mTNF)cDNA was inserted into the polylinker site of MNSM retroviral vector to create pMNSM-SV40-mTNF. Albumin enhancer/promoter (Alb e/p) sequence was used to replace SV40 early region promoter of pMNSM-SV40-mTNF vector to create pMNSM-Alb e/p-mTNF recombinant retroviral vector. The retroviral constructs were introduced into amphotropic retroviral packaging cells PA317. Production of the recombinant retroviruses was accomplished by the lipofectamine-mediated gene transfer procedure. The murine tumor cells were infected with the retroviruses in the presence of polybrene. Dot hybridization of total RNA from modified tumor cell with mTNF cDNA probe and mTNF bioassay demonstrated that transcription and expression of mTNF gene drived by Alb e/p were markedly high in the hepatoma cells which produced albumin, and inhibited in the non-hepatoma tumor cells. The hepatoma cells modified with mTNF gene lost its tumorgenicity and significantly inhibited the growth of the parental hepatoma in vivo. High-titer MNSM-Alb e/p-mTNF retroviruses or the high-titer retroviruses producing packaging cells, after intra-tumoral injection, specifcally inhibited the growth of the hepatoma, and significantly prolonged the survival period of the hepatoma-loaded mice. Biopsy and immunohistochemical assay of the hepatoma during in vivo gene therapy, showed the occurrence of extensive tumor necrosis, bleeding, and CD8+, CD25+, CD4+ lymphocytic infiltration and fibrosis.