Objective:To observe the distribution of grafted GDNF-modified neural stem cells in the rat brain with focal ischemia and to explore the neuroprotective effect.Methods:Neural stem cells(NSCs) of neonatal rat were cultured in vitro.The model of middle cerebral artery occlution(MCAO) and reperfusion was set up.Normal saline(NS),NSCs and GDNF/NSCs were transplanted into the lateral ventricle of MCAO model after reperfusion 3 days by stereotaxis instrument,Neurological severity score(NSS) tests were undertaken in all groups 1,2,3,5,7weeks respectively before and after transplantation.Immunohistochcmistry was used to check the survival and distribution of NSCs in vivo.Results:NSCs tranfected after 2 d with recombinant adenovirus containing GDNF gene launched green fluoresent and expressed nestin.NSS of GDNF/NSCs groups and NSCs groups was significantly decreased than control groups at 1～7 w after reperfusion(P

MicroRNA (miRNA) constitutes a group of small (21-23 nucleotide) noncoding RNAs that functional as posttranscriptional gene regulators.miRNA involved in the tumor development which have been suggested to play a vital role in operating to promote or suppress tumor proliferation,invasion and metastasis.The application value of miRNA as a potential biomarker was also discussed.It needs to be further explored that miRNA can be applied as biomarkers in further medical paractice.

Objective To investigate the diagnosis,differential diagnosis and treatment of gynecological acute abdomen,and provide a basis for treatment.Methods The clinical data of 749 cases with gynecological acute abdomen in our hospital were retrospectively analyzed.Results The diagnostic accuracy was 100％ of the preoperative uterine perforation,acute pelvic inflammatory disease,tubal ovarian abscess,ovarian cyst torsion and.ruptured ovarian cysts.19 cases with ovarian corpus luteum rupture were misdiagnosed for ectopic pregnancy,1 case with acute hemorrhagic salpingitis were misdiagnosed for ectopic pregnancy,1 case with IUD of ectopic pregnancy lead to pelvic abscess was misdiagnosed for ovarian cyst torsion secondary infection,the total misdiagnosis patients were 21 cases,and the misdiagnosis rate was 2.8％.217 cases with acute abdomen received the surgical treatment,532 cases were given non-surgical treatment.All patients were cured and discharged by the hospital treatment,the average hospitalization time was (8.2 ± 2.5) d.Conclusion In the diagnosis of gynecological acute abdomen,detailed ask the patients' medical history,symptoms,and comprehensive physical examination and collection of signs are important means.The understanding of acute hemorrhagic fallopian tubes,ovarian corpus luteum rupture and ovarian cyst torsion secondary infection should be further strengthened to reduce misdiagnosis rate.

Objective To survey the awareness of hyperuricemia in patients and medical personnel in Yuetan community.Methods By using random sampling method, 492 patients with hyperuricemia and 84 medical personnel in Yuetan community were selected for questionnaire survey.Results The rate of awareness of hyperuricemia in patients was 33.2%(1 472/4 428), and 95.5%(470/492)of them had the requirement of health education on hyperuricemia.The awareness rate of hyperuricemia in medical personnel was 73.0%(736/1 008), and 46.4%(39/84) of them thought health education on hyperuricemia as important or very important.Conclusion The awareness of hyperuricemia is insufficient in patients and medical personnel.Health education should be reinforced for community management on hyperuricemia.

Objective To evaluate the efficacy of community management of asymptomatic hyperuricemic patients.Methods Two hundred and six patients with asymptomatic hyperuricemia in Yuetan community of Beijing were randomly and equally divided into study group and control group.Patients in study group received 12-month standardized management, while those in control group were given conventional management.After 12 months of intervention, the clinical data and related information were analyzed and the effect of intervention was evaluated by questionnaire survey.Results After 12-month intervention, the rates of unhealthy lifestyle in intervention group were significantly lower, and the exercise time was significantly more than those in control group [drinking:frequently 8.7%(9/103)vs.11.7%(12/103),seldom 83.5% (86/103) vs.68.9% (71/103),χ2=7.005, P=0.030;eating animal offal: seldom 88.4%(91/103) vs.76.7%(79/103),frequently 1.9%(2/103) vs.8.7%(9/103),χ2=6.302, P=0.043;eating seafood: seldom 74.8%(77/103) vs.52.4%(54/103),frequently 4.8%(5/103) vs.16.5%(17/103),χ2=12.867, P=0.002;eating thickened gravy: seldom 78.6%(81/103)vs.53.4%(55/103),frequently 2.9%(3/103) vs.13.6%(14/103),χ2=16.334, P=0.000;exercise time: seldom 17.5%(18/103)vs.24.3%(25/103),1-3 h 26.2%(27/103) vs.38.8%(40/103),4-6 h 32.0% (33/103) vs.20.4%(21/103), ≥7 h 24.3%(25/103) vs.16.5%(17/103),χ2=7.852, P=0.049].The blood uric [(403±54) vs.(474±71) μmol/L], cholesterol [(4.6±0.8) vs.(4.9±1.0)mmol/L], triglyceride[(1.65± 0.54) vs.(1.86±0.86) mmol/L] and systolic pressure [(128±10) vs.(131±14) mmHg(1 mmHg=0.133 kPa)] in intervention group significantly lower than those in control group (t=7.984,P=0.000;t=2.803,P=0.006;t=2.130,P=0.034;t=2.059,P=0.041).The awareness rate of hyperuricemia-related knowledge in intervention group was significantly higher than that in control group [85.1% (964/1 133) vs.45.5%(516/1 133), χ2=390.959,P=0.000].Conclusion Standardized community management is effective for patients with asymptomatic hyperuricemia.

Diagnostic methods play important role in theoretical system of Inner Canon of Huangdi.There are 74 articles about diagnostic methods,which contains near 50 kinds of TCM diagnostic methods referred to pulse diagnosis,colour inspection and so on.This paper analyze the connotation and extension of the term "diagnostic method",establish a theoretical framework of dynamic whole concept,bi-directional discrimination,characteristics of triple pathogens and four kinds of methods.The diagnostic model of"shape-spirit-transporting qi"and the modern language"nature-biology-psychology-society" four-dimensional diagnostic method are constructed.

The present study used optical coherence tomography (OCT) to observe the relationship between inhibitor of monocyte chemoattractant protein-1 (MCP-1) and coronary in-stent restenosis after stenting ,and proved that MCP-1 is a key inflammatory factor causing in-stent restenosis after stenting .

Objective:To evaluate the safety and effectiveness of air bubble-assisted intraocular lens (IOL) implantation and its effect on corneal endothelial cell during phacoemulsification for age-related cataract.Methods:A non-randomized controlled study was conducted.One hundred and seventy eyes of consecutive 170 patients with age-related cataracts who received phacoemulsification combined with one-stage IOL implantation were included from March 2019 to September 2019 in First Affiliated Hospital of Zhejiang Chinese Medicine University in this study.Among the operative eyes, 92 eyes that underwent phacoemulsification combined with air bubble-assisted IOL implantation served as air bubble-assisted group and 78 eyes that received hyaluronan-assisted IOL implantation served as hyaluronan-assisted group.All the subjects were followed up for successive 12 weeks.The total operative duration and IOL implantation duration, corneal endothelial cell count (ECC) and the proportion of hexagonal cells before and 1, 4, 12 weeks after surgery, and intraocular pressure (IOP) before and 1 day, 1 week and 4 weeks after surgery were compared between the two groups.And the total surgery time and IOL implantation time were compared between the two groups.This study adhered to the Declaration of Helsinki and was approved by an Ethics Committee of First Affiliated Hospital of Zhejiang Chinese Medicine University (No.KL-010-01).Results:The operation went well in all the eyes and there was no serious intraoperative or postoperative complication in all the operative eyes.There was no significant difference in preoperative and postoperative ECC, the proportion of hexagonal cells and the IOP between the two groups ( Fgroup=0.004, P=0.953; Fgroup=0.706, P=0.042; Fgroup=1.896, P=0.170). The postoperative ECC and the proportion of hexagonal cells at different time points were significantly smaller than the preoperative ECC and hexagonal cells proportion in the two groups (all at P<0.05). The IOP was elevated in 2 eyes in the hyaluronan-assisted group one day after operation, which returned to normal after anterior chamber puncture.There was no postoperative high IOP in the air bubble-assisted group.The total surgery time and IOL implantation time were (357.78±36.07) seconds and (50.63±8.37) seconds respectively in the hyaluronan-assisted group, which were significantly longer than (256.95±47.74) seconds and (29.66±6.58) seconds respectively in the air bubble-assisted group, showing significant differences between the two groups (both at P<0.01). Conclusions:Air bubble-assisted technique can shorten the IOL implantation duration.Both air bubble-assisted and hyaluronan-assisted IOL implantation causes no additional risk to corneal endothelium.

Objective The objective of this study is to investigate the psychological stress status of Chinese college students ,and to provide evidence for mental health education in colleges and universities .Methods A random sampling method was conducted to perform the questionnaire (psychological stress scale for college students by Zhang Lin ,Che Wenbo ,etc .) on psychological stress a‐mong 205 college students .Results The main sources of psychological stress on college students were job‐seeking pressure ,school pressure ,academic pressure ,emotion pressure and interpersonal pressure .The scores on college environment and learning task stress of engineering and technology students were significantly higher than other divisions (P< 0 .05) .Male students showed markedly higher stress level than female students in love pressure ,inferiority pressure and academic pressure (P< 0 .05) .The scores on frustration pressure and family pressure of students with siblings were significantly higher than students from one‐child family (P<0 .05) .The scores on family pressure of rural students were significantly higher than those from towns and cities (P<0 .01) .Conclusion The psychological stress of college students in Hainan province are overall in good condition .There are some differences among different groups .We should pay more attention to college students′mental health problems ,and make separate counseling according to the profession and family background difference .

AIM To observe the effect of sodium orthovanadate on glucose and maltose absorption and to reveal its mechanism. METHODS ① Normal Wistar rats were divided into 6 groups at random, 0 9% NaCl group, acarbose group(30 mg?kg -1 ) and sodium orthovanadate high dose(16 mg?kg -1 ), middle dose (4 mg?kg -1 ) and low dose (1 mg?kg -1 )groups. The values of blood glucose of all the groups were measured with oxidation enzyme method after administration of glucose and maltose. ② The ? glucosidase was abstracted from the upper small intestine and the inhibitory effect of sodium orthovanadate on ? glucosidase was examined. RESULTS ① Sodium orthovanadate could delay the peak values of plasma glucose induced by glucose, with AUC in these groups lower than that in controls, that is (8 42?0 63) mmol?h -1 ?L -1 ( P