1.Efficacy of the Mineral Oil and Hyaluronic Acid Mixture Eye Drops in Murine Dry Eye.
Jung Han CHOI ; Jung Han KIM ; Zhengri LI ; Han Jin OH ; Kyu Youn AHN ; Kyung Chul YOON
Korean Journal of Ophthalmology 2015;29(2):131-137
PURPOSE: To investigate the therapeutic effects of mineral oil (MO) and hyaluronic acid (HA) mixture eye drops on the tear film and ocular surface in a mouse model of experimental dry eye (EDE). METHODS: Eye drops consisting of 0.1% HA alone or mixed with 0.1%, 0.5%, or 5.0% MO were applied to desiccating stress-induced murine dry eyes. Tear volume, corneal irregularity score, tear film break-up time (TBUT), and corneal fluorescein staining scores were measured at 5 and 10 days after treatment. Ten days after treatment, goblet cells in the conjunctiva were counted after Periodic acid-Schiff staining. RESULTS: There was no significant difference in the tear volume between desiccating stress-induced groups. The corneal irregularity score was lower in the 0.5% MO group compared with the EDE and HA groups. The 0.5% and 5.0% MO groups showed a significant improvement in TBUT compared with the EDE group. Mice treated with 0.1% and 0.5% MO mixture eye drops showed a significant improvement in fluorescein staining scores compared with the EDE group and the HA group. The conjunctival goblet cell count was higher in the 0.5% MO group compared with the EDE group and HA group. CONCLUSIONS: The MO and HA mixture eye drops had a beneficial effect on the tear films and ocular surface of murine dry eye. The application of 0.5% MO and 0.1% HA mixture eye drops could improve corneal irregularity, the corneal fluorescein staining score, and conjunctival goblet cell count compared with 0.1% HA eye drops in the treatment of EDE.
Animals
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Conjunctiva/*drug effects/pathology
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Cornea/metabolism
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Disease Models, Animal
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Drug Combinations
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Dry Eye Syndromes/*drug therapy/metabolism
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Emollients/administration & dosage
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Female
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Goblet Cells/drug effects/metabolism/pathology
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Hyaluronic Acid/*administration & dosage
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Mice
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Mice, Inbred C57BL
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Mineral Oil/*administration & dosage
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Ophthalmic Solutions
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Tears/*metabolism
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Viscosupplements/administration & dosage
2.Short Term Effects of Topical Cyclosporine and Viscoelastic on the Ocular Surfaces in Patients with Dry Eye.
Jun Woong MOON ; Hyun Joo LEE ; Ki Chul SHIN ; Won Ryang WEE ; Jin Hak LEE ; Mee Kum KIM
Korean Journal of Ophthalmology 2007;21(4):189-194
PURPOSE: To compare the short term effects of topical 0.05% cyclosporine (CsA) and a mixture of 0.08% chondroitin sulfate and 0.06% sodium hyaluronate (CS-HA) on dry eye ocular surfaces. METHODS: 36 patients with moderate to severe dry eye (5 mm/5 min or less with Schirmer's test or tear break up time (BUT) less than 6 seconds), were treated with topical application of CS-HA on one eye and CsA on the other 4 times a day for 6-8 weeks. BUT, Schirmer's test without anesthesia, and conjunctival impression cytology (CIC; goblet cell density, nucleus to cytoplasmic ratio, and epithelial cell morphology) were evaluated and compared between eyes before and after treatment (repeated measurement of ANOVA). RESULTS: After treatment, BUT and tear wettings were significantly prolonged in each group. Topical CsA treated eyes had greater increase in BUT (p=0.026); there was no significant difference in tear wetting (p=0.132). While the 3 parameters of CIC improved in both groups, goblet cell density was significantly higher in eyes treated with CsA (p=0.033). CONCLUSIONS: While both CS-HA and 0.05% CsA eyedrops improve ocular surfaces, topical CsA may have a better effect on enhancing tear film stability and goblet cell density.
Adjuvants, Immunologic/administration & dosage
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Administration, Topical
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Cell Count
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Chondroitin Sulfates/*administration & dosage
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Conjunctiva/drug effects/pathology
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Cyclosporine/*administration & dosage
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Drug Administration Schedule
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Drug Combinations
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Drug Therapy, Combination
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Dry Eye Syndromes/*drug therapy/metabolism/pathology
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Epithelium/drug effects/pathology
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Female
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Follow-Up Studies
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Goblet Cells/drug effects/pathology
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Humans
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Hyaluronic Acid/*administration & dosage
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Immunosuppressive Agents/*administration & dosage
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Male
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Middle Aged
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Ophthalmic Solutions/administration & dosage
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Tears/drug effects/metabolism
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Time Factors
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Treatment Outcome