Ten compounds were isolated and purified by column chromatography over silica gel, preparative TLC, and Sephadex LH-20 from the whole plant of Solanum lyratum. The structures were elucidated on the basis of physico-chemical properties and spectral data as 1beta-hydroxy-1 ,2-dihydro-alpha-santonin (1) , boscialin (2) , blumenol C (3), 3beta-hydroxy-5alpha, 6alpha-epoxy-7-megastigmen-9-one(4), dehydrovomifoliol(5) , blumenol A(6), (1'S,2R,5S, 10R) -2-(1', 2'-dihydroxy-l1'-methylethyl) -6,10-dimethylspiro[4,5] dec-6-en-8-one(7) , (1'R,2R,5S,10R)-2-( 1',2'-dihydroxy-l '-methylethyl) -6,1 l0-dimethylspiro[4,5]dec-6-en-8-one( 8) , 2-(1',2'-dihydroxy-1 '-methylethyl) -6,1 0-dimethyl-9-hydroxyspiro [4,5] dec-6-en-8-one (9) , and grasshopper ketone (10). Compounds 1-10 were isolated from this plant for the first time.
Drugs, Chinese Herbal ; chemistry ; Solanum ; chemistry ; Terpenes ; analysis ; isolation & purification

OBJECTIVETo study the alkaloids of the Chinese medicinal herbs Daphniphyllum macropodum for in search for new bioactive substances.

METHODThe CHCl3 extract of D. macropodum was subjected to repeated column chromatography on silica gel to afford four pure compounds (1-4). Their structures were determined on the basis of detailed spectroscopic analysis and comparison with the data reported in the literature.

RESULTThe four known compounds were isolated and elucidated as Daphniphyllum alkaloids with different carbon skeletons, namely longistylumphylline A (1), deoxycalyciphylline B (2), daphnicyclidin B (3) and H (4), respectively.

CONCLUSIONAll compounds were isolated from the titled plant for the first time. The discovery of daphnicyclidin B (3) and H (4) further confirmed the biogenetic relationship between the two compounds and the previously reported macropodumines A-C, found in the same plant.

OBJECTIVETo study the chemical constituents of Memorialis hirta.

METHODCompounds were isolated and purified by multiple methods, and their structures were identified based on physicochemical property and spectrum data.

RESULT12 compounds were isolated from ethyl acetate from 95% ethanol extracts of M. hirta, they were isorhamnetin (1), kaempferol (2), quercetin (3), isorhamnetin-3-O-alpha-L-rhamnopyranoside (4), kaempferol-3-O-alpha-L-rhamnopyranoside (5), isorhamnetin-3-O-beta-D-glucopyranoside (6), kaempferol-3-O-beta-D-glucopyranoside (7), quercetin-3-O-alpha-L-rhamnopyranoside (8), quercetin-3-O-beta-D-glucopyranoside (9), isorhamnetin-3-O-rutinoside (10), kaempferol-3-O-rutinoside (11) and quercetin-3-O-rutinoside (12), respectively.

CONCLUSIONAll compounds were obtained from the genus Memorialis for the first time.

OBJECTIVETo investigate the chemical constituents in herbs of Polygonum jucundum.

METHODThe 85% ethanol extract was separated by means of silica gel and Sephadex LH-20 column chromatography. The compounds isolated from the plant were identified by physicochemical properties and spectroscopic evidence.

RESULTEight compounds were isolated and identified as: quercetin-3'-O-beta-D-galactoside (1), 8-methoxyquercetin (2), pigenin (3), luteolin (4), quercetin (5), 3,5,7-trihydroxychromone (6), p-hydroxybenzaldehyde (7), beta-sitosterol (8).

CONCLUSIONAll compounds were isolated from this plant for the first time, compounds 5- 8 were isolated from the genus Polygonum for the first time.

OBJECTIVETo study the chemical constituents of Xanthium mongolicum.

METHODThe compounds were isolated with column chromatography. The structures were determined by spectroscopic techniques.

RESULTEight compounds were isolated from X. mongolicum, four compounds are sesquiterpene lactones, two compounds are triterpenes and two compounds are lignins. Their structure elucidated as xanthatin (1), xanthinosin (2), 11alpha, 13-dihydroxanthatin (3), atractylenolide III (4), oleanolic acid (5), ursolic acid (6), lignocellulose (7), balanophonin (8).

CONCLUSIONCompounds 3-8 were isolated from this plant for the first time. Sesquiterpene lactones (1-3) had shown the induction hindrance activeness of the (ICAM-1), but the activitiy of 3 was weaker.

OBJECTIVETo investigate the chemical constituents of the extract of Galium verum.

METHODThe compounds were isolated by chromatography and identified by spectral data.

RESULTThe eleven compounds obtained were identified as (+)-pinoresinol 4,4'-O-bis-beta-D-glucopyranoside (1), epipinoresinol (2), (+) -medioresinol (3), isorhamnetin (4), isorhamnetin 3-O-alpha-L-rhamnopyranosyl-(1-6)-beta-D-glucopyranoside (5), diosmetin (6), diosmetin 7-O-beta-D-glucopyranoside (7), quercetin-3-O-beta-D-glucopyranoside (8), ursolic acid (9), ursolic aldehyde (10) and rubifolic acid (11).

CONCLUSIONCompounds 1-5 and 9-11 were isolated from this genus for the first time.

OBJECTIVETo study the chemical constituents from Aeschynanthus longicaullis.

METHODThe column chromatographic techniques were applied to isolate the constituents. The spectroscopie methods were used of EI-MS and NMR to identify the structures of the separated compounds.

RESULTSeven compounds were isolated from the ethyl acetate extract of A. longicaullis, whose structures were elucidated as cryptomeridiol (1), 4 (15) -eudesmene-1beta, 6alpha-diol (2), 2,5-bornanediol (3), isovanillic acid (4), vanillic acid (5), stigmast-5 (6) , 22 (23)-diene-3beta-ol (6) and beta-sitosterol (7).

CONCLUSIONCompounds 1-5 were isolated for the first time from Gesneriaceae and 6-7 were isolated for the first time from this plant.

Twelve compounds were isolated from the herb of Chenopodium ambrosioides, and their structures were identified by spectroscopic methods as kaempferol-7-O-alpha-L-rhamnopyranoside (1), kaempferol-3,7-di-O-alpha-L-rhamnopyranoside (2), patuletin (3), quercetin-7-O-alpha-L-rhamnopyranoside (4), grasshopper ketone (5), 4-hydroxy-4-methyl-2-cyclohexen-1-one (6), syringaresinol (7), benzyl beta-D-glucopyranoside (8), dendranthemoside B (9), N-trans-feruloyl tyramine (10), N-trans-feruloyl 4'-O-methyldopamine (11), and 4-hydroxy-N-[2-(4-hydroxyphenyl) ethyl] benzamide (12). Among them,compounds 3, 6-8,10, and 12 were isolated from the genus Chenopodium for the first time, and compounds 2-12 were isolated from this plant for the first time.
Chenopodium ambrosioides ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification

OBJECTIVETo study the chemical constituents of Reineckia carnea.

METHODThe compounds were separated by means of solvent extraction, repeated chromatogramphy with silica gel, Sephadex LH-20 and MCI resin. The structures were determined by spectral analysis.

RESULTTen compounds were separated and elucidated as daucosterol (1), stigmasterol-3-O-beta-D-glu-copyranoside (2), myo-inositol(3), pentologenin (4), kitigenin (5), kitigenin5-O-beta-D-glu-copyranoside (6), neoaspidistrin (7), (1beta, 3beta, 16beta, 22S)-cholest-5-ene-1, 3, 16, 22-tetrol-1, 16-di-(beta-D-glucopyranoside) (8), rhodeasapogenin 1-0-alpha-L-rhamnopyranosyl-(1-2)-beta-D-xylopyrano-side (9), quercetin-3-O-beta-D-glucopyranosyl-(6-1)-alpha-L-rhamnopyranoside (10).

CONCLUSIONThe compounds 2, 3, 7, 9, 10 were discovered in this plant for the first time.

OBJECTIVETo study the chemical constituents of Swertia mileensis.

METHODThe air-dried whole plants of S. mileensis were extracted with 50% EtOH. The EtOH extract was suspended in H20 and extracted with petroleum ether, CHCl3 and n-BuOH successively. The compounds were isolated and purified by column chromatography from the CHCl3 fraction, and identified based on spectral analyses (MS, H-NMR, 13C-NMR).

RESULTTwelve compounds were isolated from S. mileensis, and were elucidated as 1,5, 8-trihydroxy-3-methoxyxanthone (1), 1-hydroxyl-2, 3, 5, 7-tetramethoxyxanthone (2), 1-hydroxyl-3, 5, 8-trimethoxyxanthone (3), 1-hydroxyl-2, 3, 4, 6-tetramethoxyxanthone (4), 1-hydroxyl-2, 3, 4, 7-tetramethoxyxanthone (5), 1,8-dihydroxy-3, 5-dimethoxyxanthone (6), 1, 7-dihydroxy-3, 8-dimethoxyxanthone (7), 1, 3, 5, 8-tetrahydroxyxanthone (8), balanophonin (9), oleanolic acid (10), maslinic acid (11), sumaresinolic acid (12).

CONCLUSIONCompounds 1, 3, 7-9, 11 and 12 were obtained from S. mileensis for the first time.