OBJECTIVE: To develop a specific CZE method for the determination of methamphetamine in whole blood after solid phase extraction. METHODS: With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood and the sample was analysized by CZE. RESULTS: The method showed excellent linearity and the linear correlation coefficient was 0.994. The relative standard deviation for between-day and within-day were 5.31% and 2.22%, respectively. CONCLUSION The method is effective, simple, reliable and has been used in the determination of methamphetamine in whole blood.
Doxapram/chemistry* ; Electrophoresis, Capillary/methods* ; Forensic Medicine ; Gas Chromatography-Mass Spectrometry ; Humans ; Methamphetamine/poisoning* ; Reproducibility of Results ; Sensitivity and Specificity ; Solid Phase Extraction ; Solvents/chemistry*

OBJECTIVE: To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood. METHODS: With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 (250 mm x 4.0 mm x 5 microm) column kept at 50 degrees C, the DAD detector was set at 230 nm and 250 nm. RESULTS: The limit of detection were 1-30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was > or =0.997 98. The relative standard deviation for between-day and within-day assay were <10%. CONCLUSION The method is effective, simple, reliable and has been used in real cases.
Chromatography, High Pressure Liquid/methods* ; Doxapram/isolation & purification* ; Doxepin/isolation & purification* ; Estazolam/isolation & purification* ; Forensic Medicine ; Humans ; Morphine/isolation & purification* ; Papaverine/isolation & purification* ; Pharmaceutical Preparations/isolation & purification* ; Prazosin/isolation & purification* ; Procaine/isolation & purification* ; Reproducibility of Results ; Sensitivity and Specificity ; Solid Phase Extraction/methods* ; Solvents/chemistry*