PURPOSE: By using the micro-imaging unit modified from NMR spectrometer, the high resolution MRI protocols of finer than 100 micron in 5 minutes, is sought for mouse, which plays a central role in animal studies MATERIALS AND METHODS: C57BL/6 mouse, lighter than 50 gram, is used for the experiments. The superconducting magnet is vertical type with 89 mm inner diameter at 4.9 Tesla. The diameter of rf-coil is 30 mm. Mostly used techniques are the fast spin echo and the gradient echo pulse sequence. RESULTS: For 2D images, proton density and T2 weighted images are obtained and their optimum experimental variables were sought. Minute structure of mouse brain can be recognized and 3D brain image is also obtained additionally. 3D image will be useful particularly for the dynamic contrast study using various contrast agents. CONCLUSION: Like the case of human and other small animals, the high resolution of mouse brain is enough to recognize the minute structure of it. Recently, similar studies are reported domestically, but it seems only a beginning stage. Due to easiness of breeding /control, mouse MRI study will soon play a vital part in brain study.
Animals ; Brain ; Breeding ; Humans ; Magnets ; Mice ; Protons

Objective: We sought to determine if the links between and within the default mode network (DMN) and dorsal attention network (DAT) exhibited different conditions according to catechol-O-methyltransferase (COMT) gene polymorphism in relationship to attention-deficit hyperactivity disorder (ADHD) symptoms. Methods: Fifty-seven children with ADHD and 48 healthy controls (HCs) were administered an intelligence test, the Children’s Depression Inventory, the Korean ADHD rating scale, and continuous performance test. Resting-state brain functional MRI scans were obtained, and COMT genotyping was performed to distinguish valine carriers and methionine homozygotes. Results: Compared to controls, children with ADHD showed increased ADHD scale scores, increased visual commission errors, and increased functional connectivity (FC) within the DMN and DAT. Compared to all children with ADHD, children with the methionine homozygote and those who were valine carriers showed increased FC within the DMN and DAT and decreased FC between the DMN and DAT. FC within the DMN was also increased in HC valine carriers compared to HC children with the methionine homozygote, and in children with ADHD who were valine carriers compared to HC valine carriers. Conclusion We observed increased brain connectivity within the DMN and DAT and altered brain connectivity within and between the DMN and DAT associated with COMT polymorphism in children with ADHD.

OBJECTIVE: The core deficit of attention deficit hyperactivity disorder (ADHD) is associated with frontal cortex and related circuitry. Children with ADHD and a medication history have shown atypical brain activation in prefrontal and striatal brain regions during cognitive challenge. We investigated two cognitive control operations such as interference suppression (IS) and response inhibition (RI) in children with ADHD. We also assessed the brain functions affected by the methylphenidate (MPH) effect by comparing the blood-oxygen level dependent (BOLD) signals in ADHD children on and off medication. METHODS: Eight children (9-11 years of age) with combined-type ADHD underwent rapid event-related functional magnetic resonance imaging (fMRI) during performance of a modified flanker task. Two fMRI (3.0 T) scans were conducted with a one week interval-one with MPH treatment and the other without. Functional maps were generated through group averaging and performance-based correlational analyses. RESULTS: Performances of the two cognitive control operations did not differ significantly between on-MPH and off-MPH status other than the reaction time to incongruent stimuli in ADHD children. In those affected by MPH treatment, an increased activation in the right prefrontal cortex during incongruent task was observed relative to a neutral trial in children with ADHD. CONCLUSION: On the treatment of MPH, the ADHD children exhibited increased activation of the right frontal cortex during interference suppression. This finding suggested that MPH affected the right frontal cortex in ADHD compensating for a reduced level of interference suppression. Future studies will be required to ascertain the MPH effect of cognitive brain regions among large number of children with ADHD.
Attention Deficit Disorder with Hyperactivity ; Brain ; Child ; Humans ; Magnetic Resonance Imaging ; Methylphenidate ; Phenazines ; Prefrontal Cortex ; Reaction Time

OBJECTIVE: Attention deficit hyperactivity disorder (ADHD) symptoms are associated with the deficit in executive functions. Playing Go involves many aspect of cognitive function and we hypothesized that it would be effective for children with ADHD. METHODS: Seventeen drug naive children with ADHD and seventeen age and sex matched comparison subjects were participated. Participants played Go under the instructor's education for 2 hours/day, 5 days/week. Before and at the end of Go period, clinical symptoms, cognitive functions, and brain EEG were assessed with Dupaul's ADHD scale (ARS), Child depression inventory (CDI), digit span, the Children's Color Trails Test (CCTT), and 8-channel QEEG system (LXE3208, Laxtha Inc., Daejeon, Korea). RESULTS: There were significant improvements of ARS total score (z=2.93, p<0.01) and inattentive score (z=2.94, p<0.01) in children with ADHD. However, there was no significant change in hyperactivity score (z=1.33, p=0.18). There were improvement of digit total score (z=2.60, p<0.01; z=2.06, p=0.03), digit forward score (z=2.21, p=0.02; z=2.02, p=0.04) in both ADHD and healthy comparisons. In addition, ADHD children showed decreased time of CCTT-2 (z=2.21, p=0.03). The change of theta/beta right of prefrontal cortex during 16 weeks was greater in children with ADHD than in healthy comparisons (F=4.45, p=0.04). The change of right theta/beta in prefrontal cortex has a positive correlation with ARS-inattention score in children with ADHD (r=0.44, p=0.03). CONCLUSION: We suggest that playing Go would be effective for children with ADHD by activating hypoarousal prefrontal function and enhancing executive function.
Attention Deficit Disorder with Hyperactivity* ; Brain* ; Child* ; Depression ; Education ; Electroencephalography ; Executive Function ; Humans ; Neurobehavioral Manifestations ; Prefrontal Cortex

INTRODUTION: The gamma-aminobutyric acid type A (GABAA) receptor is an important pharmacological target of alcohol. The phamacological characteristics of the receptor are largely determined by its subunit composition. Compared with all other alpha subtypes, the alpha6- containing receptors are more sensitive to GABA and less sensitive to benzodiazepines. The purpose of this study was to address a role for GABAAalpha6 receptor subunit gene in the development of alcohol dependence. The differential manifestation of alcohol withdrawal symptoms related to GABAAalpha6 polymorphism in patients treating with benzodiazepines was also examined. METHODS: Eighty-seven inpatients with alcohol dependence, and sixty healthy controls were evaluated using CIWA-Ar scale. Each patient was genotyped for GABAAalpha6 subunit. Association between GABAAalpha6 polymorphism and severity of withdrawal symptom were determined. RESULTS: No significant difference was found in GABAAalpha6 receptor genetic type and allelic distribution between the alcohol dependent and control subject. Tremor was more severe in CC than TT type. TT type had higher degree of anxiety, agitation and headache than CC type. The GABAAalpha6 C allele increased the average score of tremor significantly, and T allele increased that of agitation. CONCLUSION: The results suggested that GABAAalpha6 genetic polymorphism was not associated with alcohol dependence and with severity of alcohol withdrawal symptoms. But in benzodiazepine treated patients, GABAAalpha6 polymorphism and allelic type show the difference in severity of each withdrawal symptom. These differences of severity are partly responsible for the unique pharmacological properties associated with the GABAAalpha6 subunit.
Alcoholism ; Alleles ; Anxiety ; Benzodiazepines ; Dihydroergotamine ; gamma-Aminobutyric Acid ; Headache ; Humans ; Inpatients ; Polymorphism, Genetic ; Receptors, GABA ; Substance Withdrawal Syndrome* ; Tremor

OBJECTIVES: In order to improve the life satisfaction, we should be aware of psychological factors associated the life satisfaction. Therefore, the purpose of this study was to examine the effect of depression, anxiety, temperament, and character on life satisfaction in college students. METHODS: A total 314 participants completed questionnaires, which included Satisfaction with Life Scale, Beck Depression Inventory-II, Beck Anxiety Inventory, The Rosenberg Self-Esteem Scale and Temperament and Character Inventory-Revised. To evaluate the difference according to social factor, t-test and one-way analysis of variance were performed. To analyze the effect of psychological characteristics on life satisfaction, stepwise multiple regression analysis was used. RESULTS: Life satisfaction was significantly different according to only gender of the social factors. Life satisfaction was positively correlated with self-esteem, self-directedness, and persistence (P). Conversely, depression, anxiety, harm avoidance, and novelty seeking (NS) were negatively correlated. Stepwise multiple regression revealed that depression, self-esteem, P, NS, and self-transcendence were factors affecting life satisfaction in university students. CONCLUSION: This result shows that life satisfaction is influenced by depression, self-esteem, temperament, and character. The findings of this study suggest that to improve the life satisfaction requires not only preventing and treatment of depression but also identifying the psychological characteristics, such as temperament and character.
Anxiety ; Depression ; Humans ; Surveys and Questionnaires ; Temperament

OBJECTIVES: Among chemical and non-chemical dependence, internet addiction is one of the most frequent addictive behavior in adolescent. This study was performed to detect certain temperamental and genetic characteristics in adolescent with internet addiction tendency. METHODS: 87 adolescents with internet addiction tendency (AIT) and 78 adolescents without internet addiction tendency (NAIT) were classified by Young's Internet Addiction Test. Cloninger's Temperament Character Inventory and 6 candidate genes of drug addiction (DRD2 TaqI A, DRD4, DAT1, 5-HTTLPR, NET-8, and COMT) polymorphisms were tested between two groups. RESULTS: Novelty Seeking scores (p=0.001) and Harm Avoidance scores (p=0.042) were significantly higher in AIT. The frequency of DRD2-A1/A1 genotype and G allele of NET-8 in AIT was higher than that in NAIT. In AIT, Novelty Seeking scores were significantly different (p=0.011) according to NET-8 genotypes (G/G>G/A>A/A). CONCLUSION: These data suggest that male adolescents with internet addiction tendency have the specific temperament and genetic polymorphisms like chemical dependence.
Adolescent* ; Alleles ; Behavior, Addictive ; Genotype ; Humans ; Internet* ; Male* ; Polymorphism, Genetic* ; Substance-Related Disorders ; Temperament*

with DAT-9 gene allele. And The total score of CIWA-Ar scale in the subject without DAT-9 gene allele was significantly higher than in the subject with DAT-9 gene allele. COMT: The total score of CIWA-Ar scale in heterozygote was significantly higher than in homozygote. CONCLUSION: Our results suggest the relationship between specific genetic factors and the withdrawal symptoms of alcohol dependent patients. As the candidate gene of the severity of alcohol withdrawal syndrome, DRD2 Taq1 gene was recommended.
Alcoholism* ; Alleles ; Heterozygote ; Homozygote ; Humans ; Polymorphism, Genetic ; Substance Withdrawal Syndrome*

OBJECTIVE: Repetitive transcranial magnetic stimulation (rTMS) was approved by the Food and Drug Administration to alleviate symptoms of treatment-resistant depression. This study aimed to evaluate the effectiveness of rTMS treatment on alleviating occupational stress by evaluating clinical symptoms and quantitative electroencephalography (QEEG). METHODS: Twenty-four health care workers were randomized to receive 12 sessions of active or sham rTMS delivered to the left dorsolateral prefrontal cortex (DLPFC). Each session consisted of 32 trains of 10 Hz repetitive TMS delivered in 5-second trains at 110% of the estimated prefrontal cortex threshold. Before and after the intervention, the Korean version of the occupational stress inventory (K-OSI), Beck's depression inventory (BDI), and Beck's anxiety inventory (BAI) were administered and EEG was performed using a 21-channel digital EEG system. RESULTS: After TMS, the average scores for the affective responses to stressors on the personal strain questionnaire (PSQ) subscale of K-OSI and BDI decreased significantly for the active-TMS group compared to the sham-TMS group. Also, the active-TMS group showed a significantly greater decrease in relative alpha in the F3 electrode and a significantly greater increase in the F4 electrode. CONCLUSION: High-frequency rTMS on the left DLPFC had stress-relieving and mood-elevating effects in health care workers, likely by stimulating the left frontal lobe.
Anxiety ; Delivery of Health Care* ; Depression ; Electrodes ; Electroencephalography ; Frontal Lobe ; Humans ; Pilot Projects* ; Prefrontal Cortex ; Transcranial Magnetic Stimulation* ; United States Food and Drug Administration

Recently, the correlation between on-line game playing and aggressiveness has been an important and controversial issue, especially in children and adolescents. The current article summarized the hypothesis of on-line game playing and aggressiveness in terms of neutral view, including General Aggression Model, meta-analysis, and the limitations of both theories. In addition, we reviewed brain studies of on-line game playing and the limitation of these studies, such as cross-sectional approach, small number of subjects, and variable etiologies of aggressiveness. We suggest that cohort study, large number of subjects, genre of game, environmental factors, and various etiologies of aggressiveness should be considered in studies of on-line game playing and increased aggressiveness.
Adolescent ; Aggression ; Brain ; Child ; Cohort Studies ; Humans ; Internet ; Meta-Analysis as Topic ; Video Games