OBJECTIVE To optimize hematopoietic stem cell transplantation therapy and provide support for drug research by investigating the dynamic process of hematopoietic and immune system reconstitution after bone marrow transplantation(BMT)in mice.METHODS CD45.2+C57BL/6 mice were used as recipient mice and randomly divided into the normal control group and transplantation group,with 30 mice in each.The transplantation group was irradiated by a lethal dose of cobalt-60 rays.Bone marrow cells were prepared from CD45.1+C57BL/6 mice and transfused into recipient mice through the tail vein.Peripheral blood,spleens,lymph nodes,thymuses and bone marrow were collected at 1,2,4,8 and 16 weeks after transplantation.Blood routine examination was performed with peripheral blood and total cell numbers in suspensions of other organs were counted by an automated cell counter.Cell classification analysis of white blood cells in peripheral blood,cell suspensions of other organs was performed by flow cytometry.RESULTS Four weeks after BMT,the numbers of white blood cells and red blood cells in peripheral blood of recipient mice returned to the same level of or higher level than normal control(P<0.05).Although the number of platelets recovered significantly,it was still mark-edly lower than that of normal control until 16 weeks post BMT(P<0.05).In addition,the percentages of myeloid leukocytes and B cells in peripheral blood,spleens,lymph nodes,and bone marrow,as well as megakaryocytes and erythrocyte progenitor cells in bone marrow also returned to normal,and the majority of myeloid leukocytes and B cells were CD45.1+cells from the donors.Eight weeks after BMT,T cells in peripheral blood,spleens,lymph nodes,thymuses,and bone marrow of recipient mice returned to normal,and CD45.1+T cells were dominating.CONCLUSION The hematopoietic and immune reconstitution of recipient mice is nearly completed eight weeks after BMT.However,the reconstruction speed of different kinds of cells and the reconstruction status of same kind of cell in different organs vary widely.

Objective:To elucidate the molecular mechanism(s) by which methyl salicylate enhances the skin de-livery of herbal ingredients with diverse lipophilicity.Methods:The toxicity of methyl salicylate on skin cell lines was evaluated using the MTT assay.The Franz diffusion cell method was used to measure the permeability enhancing activities of methyl salicylate for five herbal ingredients with a range of lipophilicities.The interaction between methyl salicylate and the stratum corneum (SC) was observed by using an infrared spectroscopy technique.Moreover,the solu-bilities and SC-vehicle partition coefficient were determined to monitor the impact of methyl salicylate on the drug thermodynamic activities and partition into the SC layer,respectively.Results:Compared with azone (1-dodecylazacycloheptan-2-one),methyl salicylate showed lower toxicity to skin cells in terms of the ICso values.The in vitro skin permeation studies showed that methyl salicylate could greatly improve the cumulative amounts or steady state flux of the selected model drugs with the exception of osthole,which indicated that methyl salicylate was prone to promote the skin delivery of hydrophilic drugs.The Fourier transform infrared spectroscopy studies revealed that methyl salicylate mainly interacted with SC lipids,leading to the disruption of the orderly arrangement of the SC.In addition,methyl salicylate had no obvious effect on the drug thermodynamic activity and partition into the SC.Conclusion:Methyl salicylate could effectively promote the skin delivery of relatively hydrophilic in-gredients in externally used traditional Chinese medicines (TCM) without obvious cytotoxicity.