Female ; Hepatitis B virus ; Hepatitis B, Chronic ; drug therapy ; Humans ; Pregnancy

Adolescent ; Adult ; Child ; Child, Preschool ; Hepatitis B, Chronic ; diagnosis ; therapy ; Humans ; United Kingdom

Different subtypes of chronic hepatitis B virus(HBV)infection occurred clinically because of the status of virus’s interaction with immune system. In patients with immune tolerance,high level of HBV DNA was found,serum hepatitis B e antigen(HBeAg)was positive,and serum level of alanine aminotransferase(ALT)was normal with only mild or no inflammation in liver tissue. However,not all the patients with normal ALT were in immune tolerance status,the disease could progress insidiously and develop into liver cirrhosis. Whether these patients need anti-viral therapy has always been a hotspot of study. This article reviewed the correlation between immune status and HBV infection,the identification of patients with normal ALT but not in immune tolerance status,and the indication of anti-viral therapy for these patients.

It has been nearly 20 years from the discovery of hepatitis C virus (HCV)and clinical diagnosis of hepatitis C to antiviral therapy with interferon,and significant progress has been made.Substantial changes have taken place in both the concept and strategy for antiviral treatment of hepatitis C,with more and more direct-acting antiviral agents emerging in recent years;these changes are as follows:from the control of HCV to clinical cure,from a sustained virologic response (SVR)rate around 30%with conventional interferon therapy to an SVR rate of 70%-80% with standard therapy with pegylated interferon and ribavirin,and from evaluating the difficulty of treatment based on genotypes and viral load to determining the treatment strategy according to host IL28B genotypes and response patterns (rapid virologic re-sponse and complete early virologic response).The progress in antiviral therapy strategies for hepatitis C is systematically reviewed for re-understanding of refractory hepatitis C.

Hepatitis C virus (HCV) is one of the major causes of acute and chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Laboratory diagnosis is essential for the diagnosis of HCV infection. Laboratory etiological diagnosis of chronic HCV infection includes HCV antigen and antibody detection and quantitative detection of HCV RNA. Quantitative detection of serum HCV RNA is the only laboratory diagnosis index for current HCV infection and plays a very important role in determination of treatment time, selection of treatment regimens, and evaluation of therapeutic effect of antiviral treatment. This paper elaborates on the target population, detection methods, and clinical significance in laboratory screening for chronic HCV infection, so as to provide timely diagnosis and effective treatment of chronic HCV infection.

The main transmission route of chronic hepatitis B virus infection is mother-to-child transmission of hepatitis B virus and the main cause of combined immune prophylaxis failure in neonates at the end of pregnancy is high viral load. Moreover, oral administration of nucleos(t)ide analogues (NAs) during the second and third trimesters of pregnancy can significantly reduce or even completely block mother-to-child transmission of HBV. This article focuses on the necessity and feasibility of oral NAs antiviral therapy for HBV carrier pregnant woman with high viral load, and the issues commences at the time of medication and viral load thresholds.

Hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma (HCC) in China. The occurrence of HCC can significantly be reduced with effective long-term antiviral treatment. Since the widespread clinical use of nucleos(t)ide analogues, such as entecavir and tenofovir that has a strong potency and high genetic barrier to resistance; the detection rate of HBV DNA in serum of patients with chronic hepatitis B is no more than 85% ~ 95%, but HCC can still occur in a small number of patients. This article will review whether the timing and selection of NAs treatment differ to prevent and reduce the incidence of HCC.

Hepatitis B virus (HBV) infection is a major public health problem worldwide. With the extensive prophylactic vaccination with hepatitis B vaccine for neonates and an increasing number of patients receiving antiviral treatment, remarkable achievements have been made in HBV infection among Chinese children. However at present, there is still a high HBsAg-positive rate among the adults in China, and with more and more special populations, it is necessary to perform timely screening and diagnosis and give antiviral therapy as early as possible, so as to prevent or delay the progression to liver cirrhosis, liver failure, and hepatocellular carcinoma after HBV infection. The selection of treatment timing and drugs should not only focus on HBV virology and liver function, but also consider chronic underlying diseases, mother-to-child transmission, and reactivation of HBV in patients receiving chemotherapy and immunosuppressants, thereby realizing the timely screening, diagnosis, and treatment of the population at a high risk of HBV and special populations and bringing more benefits to patients.

OBJECTIVETo investigate the Pre-Core mutations of HBV and clinical significance among families with history of chronic HBV infection.

METHODSThe G to A mutation at nucleotide 1896 in the pre C gene of HBV DNA was detected by PCR-RFLP.

RESULTSThe results showed that the mutation rate of G to A mutation at nucleotide 1896 in the pre C gene of HBV DNA was much higher in patients (56.3%) and their family members (40.5%) than in their spouses (25.0%). While the positive rate of anti-HBs in their spouses were 26.3%. On the other hand those mutations were much higher in chronic hepatitis B patients (52.4%) and HBV carriers (44.4%) than in chronic severe hepatitis B patients (20.0%).

CONCLUSIONSThe G to A mutation at nucleotide 1896 in the pre C gene of HBV DNA could be associated with persistent HBV infection.

Adolescent ; Adult ; Child ; China ; epidemiology ; Female ; Genes, Viral ; genetics ; Hepatitis B virus ; genetics ; Hepatitis B, Chronic ; epidemiology ; virology ; Humans ; Male ; Middle Aged ; Point Mutation ; Polymorphism, Restriction Fragment Length

Animals ; Enzyme Inhibitors ; pharmacology ; Hepatitis ; immunology ; Immune Sera ; pharmacology ; Mice ; Sodium-Potassium-Exchanging ATPase ; antagonists & inhibitors ; metabolism