Objective:To compare the clinical application effect of polyetheretherketone (PEEK) and titanium in cranioplasty.Methods:Sixty patients with skull loss in Shengjing Hospital of China Medical University from January 2015 to December 2019 were retrospectively analyzed. Among them, 25 patients were repaired with PEEK material (PEEK group) and 35 patients were repaired with titanium mesh (titanium mesh group). The postoperative complications within 6 months, plastic surgery satisfaction, hospital stay and hospitalization cost were compared between 2 groups.Results:There were no significant differences in bleeding, infection, epilepsy, implant displacement, implant exposure, incidence of secondary operations and the length of hospital stay between 2 groups ( P>0.05). The incidence of subcutaneous fluid, good plastic surgery satisfaction and hospitalization costs in the PEEK group were significantly higher than those in titanium mesh group: 28.0% (7/25) vs. 5.7% (2/35), 88.0% (22/25) vs. 62.9% (22/35) and (12.4 ± 1.5) × 10 4 yuan vs. (7.0 ± 2.4) × 10 4 yuan, and there were statistical differences ( P<0.05 or<0.01). Conclusions:PEEK material is safe and effective in cranioplasty, and the treatment satisfaction is better, but the cost is higher. PEEK can be used as an ideal skull repair material for patients with economic basis.

To make a systematic study on acupoints selection and medication rules for allergic rhinitis treated with acupoint application, literature regarding clinical researches on acupoint application for allergic rhinitis since 2000 has been retrieved; medication and acupoints involved were preprocessed and cluster analysis technique was applied to classify the data. As a result, a total of 37 pieces of literature were included, involving 27 types of herbs and 33 acupoints. The results showed Baijiezi (Brassica alba Boiss), Xixin (Radix et Rhizoma Asari), Gansui (Radix Kansui), Yanhusuo (Corydalis), Jiangzhi (Ginger juice) and Baizhi (Radix angelicae) constituted the main herb prescription, and the layer was increased along with frequency of use. Feishu (BL 13), Dazhui (GV 14), Shenshu (BL 23), Pishu (BL 20), Gaohuang (BL 43), Fengmen (BL 12) constituted the main acupoint prescription, which were clustered into 4 classes; Feishu (BL 13) and Dazhui (GV 14) constituted the first class; Pishu (BL 20) and Shenshu (BL 23) constituted the second class; the first and second class merged into the third class, Including Feishu (BL 13), Dazhui (GV 14), Shenshu (BL 23) and Pishu (BL 20); Gaohuang (BL 43) and Fengmen (BL 12) constituted the fourth class; the fourth class and the third class constituted the whole acupoint prescription. It was showed that for the allergic rhinitis treated with acupoint application, the medication was mainly epispastic herbs with pungent nature that belonged to the lung, spleen and kidney meridian, which presented the principle of simultaneous treatment of principal and subordinate symptoms; also the medication can be selected from core layer to supporting layer based on clinical needs; for the selection of acupoints, acupoints in lung and bladder meridian were mainly selected to assist exterior and resist the pathogenic qi, which could treat allergic rhinitis fundamentally by joint use with acupoints in soleen and kidney meridian.
Acupuncture Points ; Data Mining ; Databases, Bibliographic ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; Humans ; Meridians ; Rhinitis, Allergic ; drug therapy

Clinical literature of bronchial asthma treated with acupoints application from January 2000 to March 2014 in modern periodicals databases was retrieved through computer. With cluster analysis and frequency analysis methods of data mining, acupoints selecting and medication rules of bronchial asthma treated with acupoints application were analyzed. Total 38 articles were included eventually, including 25 acupoints and 42 medicines. The results indicate that on acupoints selecting, Feishu (BL 13) is used as the main acupoint and 3 groups of bladder meridian and conception vessel acupoints are applied alternately and on medicines, Baijiezi (Brassica alba Boiss), Xixin (Radix et Rhizoma Asari), Gansui (Radix Kansui), Yanhusuo (Corydalis) and Mahuang (Radix et Rhizonma Ephedrae) are primarily adopted, epispastic medicines being the main medicines; medicines mostly belong to lung meridian, main medicines being unchanged mostly with Shengjiang as guiding drug.
Acupuncture Points ; Acupuncture Therapy ; Anti-Asthmatic Agents ; therapeutic use ; Asthma ; drug therapy ; therapy ; Data Mining ; Databases, Factual ; Humans ; Meridians

Objective To observe the effects of secreted protein,acidic and rich in cysteine (SPARC) on the expression of TGF-β1 and collagen type Ⅰ in cultured human keloid fibroblasts by real-time fluorescence quantitative RT-PCR.Methods In vitro keloid fibroblasts were stimulated by different concentrations of recombinant human SPARC,and with the control group for comparison,real-time fluorescence quantitative RT-PCR to detect expression of TGFβ1 and collagen type Ⅰ.Results Compared with the control group,the expression of TGF-β1 and collagen type Ⅰ was significantly increased in the experimental group.Conclusions SPARC could enhance the expression of TGF-β1 and collagen type Ⅰ in keloid fibroblasts significantly.

Objective To explore the clinical features,diagnosis and treatment of serious pyogenic infection in deep neck.Methods The retrospective analysis was taken on clinical data of 49 cases.They were all with the deep neck space infections.Among them,19 cases were revealed with diabetes,and only 7cases had the treatment history of diabetes before.Results After systemic anti-inflammatory and local symptomatic treatment,the mean course of treatment in patients without diabetes was 10.1 days,and in patients with diabetes was 18.1 days.Conclusion For diabetic patients with more severe neck space infection,in the control of blood glucose under the premise of local treatments and systemic therapy were equally important.

Objective: To observe left and right ventricular mechanical sequence in systole and diastole by dual Doppler Echocardiography in healthy subjects. Methods: Dual Doppler echocardiography was performed in 100 normal subjects with dual-outflow-tract-view and apical four chamber view to simultaneously record the spectrum of left/right ventricular outlfow tract (LVOT)/(RVOT) and left/right ventricular inlfow tract (LVIT)/(RVIT) at the same cardiac cycle. The time cycles of blood lfow spectrum from the peak of QRS complex to aorta, pulmonary artery, mitral valve (MV) and tricuspid valve (TV) were measured, the starting time differences for blood lfow spectrum of aorta and pulmonary artery, MV and TV were calculated to assess the mechanical sequence of left and right ventricle in systole and diastole respectively. Results: There were 48/100 (48%) subjects having LV ejection preceded than RV (95% CI 0.38-0.58), 46 (46%) having RV ejection preceded than LV (95% CI 0.36-0.56) and 6 (6%) having LV, RV simultaneous ejection (95% CI 0.01-0.11). There were 96/100 (96%) subjects having RV iflling prior to LV (95% CI 0.90-0.99), 3 (3%) having LV iflling prior to RV (95% CI 0.01-0.08) and 1 (1%) having LV, RV simultaneous iflling (95% CI 0.00-0.05). Conclusion: There is a regular pattern for mechanical sequence of LV and RV in systole and diastole in healthy subject; in systole, the sequence of LV varies from person to person, while in diastole, RV is always preceded. Based on normal ventricular mechanical sequence, optimizing programming parameter of pace maker and choosing cardiac sequence in diastole might be the ifnal criteria for cardiac resynchronization therapy in the future.

The mutual influence of lead and cadmium ions on “in-situ” bismuth film coated glassy carbon electrode was demonstrated using different concentration target ions by changing ratio between Pb2+ and Cd2+. It is found that the presence of coexisting ions can have an influence on the detection of target ions. Especially for the detection of Cd2+, the existence of Pb2+ can be beneficial for the deposition of Cd due to the more positive deposition potentials. And the analytical performances of Cd2+and Pb2+ were obtained. The detection limit is 0. 5 μg/L towards Pb2+ with a linear range from 1-80 μg/L using 60 s deposition time. The resulting calibration plots of Cd2+ are linear over the range from 1-25 and 30-200 μg/L with the detection limit of 1 μg/L using 120 s deposition time. The bismuth film was finally employed to determine the Pb2+and Cd2+in real sample with good satisfied results.

Objective To understand the phenotype and enzyme genotype of pan‐drug resistant carbapenemase‐producing Acine‐tobacter baumannii to provide the evidence for clinical rational use of antibiotics and monitoring hospital infection .Methods A total of 117 clinically isolated strains of Acinetobacter baumannii were collected and performed the routine microbiological detection . Multi‐drug resistant Acinetobacter baumannii was screened by K‐B disk diffusion method .The phenotype of carbapenemase‐produ‐cing strains was detected by using the Carba NP colorimetry and modified Hodge test .The drug resistant genotype of multi‐drug re‐sistant Acinetobacter baumannii was verified by PCR .Results Among clinically isolated 117 strains of Acinetobacter baumannii ,64 strains were multi‐drug resistant Acinetobacter baumannii ,in which 33 strains were carbapenemase positive .OXA‐23 drug‐resistant genotype of carbapenemase was detected by PCR ,while IMP ,VIM and NDM‐1 drug resistant genes were not detected .Conclusion The CarbaNP method can rapidly detect carbapenemase‐producing strains with the advantages of strong sensitivity and simple oper‐ation ,which conduces to improve the detection rate of carbapenemase‐producing strains and monitor the nosocomial infection .

Being an important basic medical course, Pathogenic biology is a bridge discipline to connect basic medicine with clinical medicine. Experiment teaching of pathogenic biology has auxiliary function for grasping and understanding the theory of knowledge. As a new teaching mode,microlecture brings more changes for classroom teaching. Microlecture video has a positive role in arousing students’learning interest because of some characteristics including short,vivid,visual and auditory coordination. This article disccussed the advantages of teaching ideas and methods of microlecture, combined with experimental course contents and features of pathogenic biology to introduce the application and function of microlecture in experimental teaching of pathogenic biology.

Objective: To study the clinical results of Coflex and lumbar posterior decompression and fusion in the treatment of lumbar degenerative spondylolisthesis at L_4-5. Methods: Thirty-eight patients with Grade Ⅰ degenerative spondylolisthesis, from January 2008 to December 2011 in Beijing Chaoyang Hospital, Capital Medical University were reviewed, and patients were divided into two groups by randomness. Group A was treated with Coflex and group B with pedicle instrumentation and interbody fusion. Fifteen patients were included in group A, and 23 patients were included in group B. In group A, the average age was (56.3±9.1) years. In group B, the average age was (58.2±11.2) years. The clinical results were evaluated by visual analogue scale (VAS) and Oswestry disability index (ODI). Slip distance (SD) was measured before and after surgery, and the changes of intervertebral angle at index level and adjacent level were also recorded. Results: The follow-up period was 36 to 68 months, with the average of (39±14) months in the both groups. The operation time and bleeding volume of patients in group A were significantly less than that of group B (P<0.05). In both groups, the difference of ODI and VAS before operation and postoperative follow-up were statistically significant (P<0.05). There was no significant difference between lumbar intervertebral angle and the sliding distance in group A at all time points. In the group B, there was a significant increase in the intervertebral angle and the sliding distance at L_3-4 and L₅-S₁ level after surgery, the difference at upper and below adjacent segment before and after surgery were statistically significant. Conclusions Coflex interspinous dynamic stabilization system has same excellent clinical results as pedicle screw instrumentation and fusion surgery for the treatment of L_4-5 degenerative spondylolisthesis; no significant progression of spondylolisthesis been observed during more than 3 years follow-up, and no obvious adjacent segment degeneration has been found.