OBJECTIVE:To promote the standardization of the packing inserts of Chinese traditional patent medicine in the writing and printing. METHODS: A total of 106 package inserts of 5 common dosage forms of Chinese traditional patent medicine analyzed in accordance with the national standard. RESULTS: Problems were found in most of the package inserts of Chinese traditional patent medicine, which manifested as chaotic ranking of essential components, non-standard stating of actions and indications, unclear stating about dosage and administration, nonobjective in the labeling of adverse drug reactions, confusion between contraindication and precautions, lacking of research document on drug interactions, pharmacology & toxicology and pharmacokinetics. CONCLUSION:The drug surveilance agency should attach importance to and tighten control on the nonstandard writing and printing of packing inserts of Chinese traditional patent medicine.

Objective Poly (ADP-ribose) polymerase (PARP) have been demonstrated to play an important role in systemic lupus erythematosus (SLE). In this study we assessed the expression of PARP on peripheral blood mononuclear cells with active or inacte SLE and tried to investigate the relationship between PARP and cell apoptosis on SLE. Methods Thirty definitive SLE patients and 10 healthy controls were enrolled. PBMC were separated from the peripheral blood samples. Western blot technique was applied to analyze the expression of PARP. Flow cytometry were applied to analyze the cell apoptosis. T test were used. Results The cell apoptosis in active patients with SLE was significantly higher than that of inactive patients with SLE and normal controls (the t values were 4.83 and 5.05 respectively, P<0.05). The level of PARP expression was significantly decreased in active patients with SLE as compared with controls and inactive patients with SLE (the t values were 7.66 and 7.07 respectively, P<0.05). At the same time, the cleavage fragment of PARP increased in active patients. Conclusion The results suggest that PARP may be involved in the pathogenesis of SLE and the expression level may be a good indicator for disease activity of SLE . Decrease of PARP may promote the occurrence and development of SLE. In addition, PARP may play a certain role in adjusting cell apoptosis in patients with SLE.

Objective To explore the expression of Akt in peripheral blood mononuclear cells (PBMCs) and its regulatory role on its downstream molecules forkhead box transcription factor O1 (FOXO1) and Bim in patients with systemic lupus erythematosus (SLE).The role of Akt/FOXO1/Bim signaling pathway on PBMCs apoptosis was clarified.Methods Sixty-three SLE patients and 23 healthy controls were enrolled.PBMC were separated from the peripheral blood specimen.Western blot technique was applied to analyze the expression of Akt,FOXO1 and Bim.Flow cytometry was applied to analyze PBMCs apoptosis.The t-test was used for statistical analysis.Results The apoptosis of PBMCs in patients with active SLE [(16.3±4.0)％] was significantly higher than that of patients with inactive SLE [(5.6 ±2.9)％] and normal controls [(5.2 ±4.2)％,t=4.83,5.05;P＜0.05].The levels of phosphorylation of Akt,FOXO1 and Bim expression was significantly decreased in patients with active SLE as compared with controls and patients with inactive SLE (P＜0.05).However,these indicators had no significant difference between the controls and patients with inactive SLE (P＞0.05).Conclusion ① The results of this study suggest that the expression level of Akt may be a good indicator for disease activity of SLE.Decrease of Akt may promote the occurrence and development of SLE.② Akt activity decline may lead to increased apoptosis of PBMCs.In this process,Akt may regulate its downstream FOXO1 and Bim phosphorylation levels which can enhance their ability to pro-apoptotic.

ObjectiveTo study the prevalence rate of post-stroke depression (PSD) of the elderly in the community and analyze risk factors of them. MethodsThe elders of the age of 60 or over were sampled as subjects from a community. Zung (SDS) was used as screening instruments and Hamilton rating scale for depression (HRSD-24) as evaluating scales. The prevalence rate of PSD was surveyed. A structured questionnaire was used and physical examination was carried out. Risk factors measured included baseline information, daily habits, and history of main diseases (hypertension, coronary heart disease, diabetes mellitus, myocardial infarction, and so on). The relationship between risk factors and PSD was analyzed.ResultsThe prevalence of PSD was 9.5%. The risk factors for PSD were life quality and age. ConclusionPost-stroke depression disturbs elder's health and rehabilitation. The prevalence rate and harm of PSD are higher than depression without stroke.

Objective To summarize the management of cardiopulmonary bypass(CPB) for infants below 10 kilograms with congenital heart diseases.Methods From Jan 2010 to Apr 2011,the clinical datas of 122 infants aged from 1.5 months to 2 years with body weight 3 to 10 kilograms,who underwent open heart surgery under mild or moderate hypothermia CPB were retrospectively analyzed.Results Among all the 122 infants,CPB time was 13 ～ 118 min [(62.69 ± 21.48) min],aortic cross-clamp time was 0 to 86 min [(35.47 ± 19.51) min].All patients were spontaneous resuscitation and successfully weared from the machine,no severe complications associated with CPB occurred,3 infants died (2.46％,3/122) after operation.Conclusion Using membrane oxygenator,circuit tubing and artery filter with less priming,and highflow perfusion during CPB,maintaining hemodynamics stable,holding reasonable hematocrit and colloid osmotic pressure,good myocardial protection and ultrafiltration are the significant elements of the management of CPB in infants with weight less than 10 kilograms.

Objective To study the expression of transcription factor FOXO1 and FOXO3a on peripheral blood mononuclear cells (PBMC) in patients with active or inactive systemic lupus erythematosus (SLE) and investigate the effect of FOXO1 and FOXO3a on the clinical features of SLE. Methods Thirty SLE patients and 10 healthy controls were enrolled. PBMC were separated from the peripheral blood. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot were applied to analyze the expression of FOXOI and FOXO3a. Results The level of FOXO1 expression was significantly decreased in active SLE patients compared with controls and patients with inactive SLE (P<0.05). The level of FOXO1 expression in inactive SLE patients was lower than that of the controls (P<0.05). The expression of FOXO1 mRNA was negatively correlated to SLEDAI. However, the level of FOXO3a was similar among the three groups. Conclusion The result suggests that FOXO1 may be involved in the pathogenesis of SLE and the expression level of FOXOI may be a good indicator for the disease activity of SLE.

Objective:To investigate the role of scavenger receptor BⅡ(SR-BⅡ) in the oxidized low density lipid(ox-LDL) induced foam cells formation promoted by porphyromonas gingivalis(P.g).Methods:Peritoneal macrophages were stimulated with P.g and ox-LDL,and then foam cells formation were checked.The expression of SR-BⅡ was detected by Western blot and real-time PCR.Next,siRNA targeting SR-BⅡ was used to detect the change of foam cells formation.Results:After being stimulated with P.g and ox-LDL,the foam cells formation was significantly increased.During the process of foam cells formation,P.g infection increased the expression of SR-BⅡ.And the knockdown of SR-BⅡ by siRNA significantly reduced the foam cells formation.Conclusion:P.g infection can increase the expression of SR-BⅡ and the regulation of SR-BⅡ expression can change the foam cells formation.

BACKGROUND: Peripheral nerve injuries are hoped to promote the regeneration of nerve repair by elevating brain-derived neurotrophic factor (BDNF) concentration in local injury regions. OBJECTIVE: To observe the recanalization of nerve fiber and motor function in sciatic nerve injury rats following BDNF gene-modified bone marrow mesenchymal stem cells (BMSCs). DESIGN, TIME AND SETTING: The randomized, controlled animal study was performed at the Fujian Institute of Neurology from May 2007 to May 2008. MATERIALS: Femur and tibia of F344 male rats aged 2 months were sterilely harvested to prepare BMSCs. BDNF gene-modified BMSCs were prepared using constructed chronic viral vector PNL-BDNF-IRES2-EGFP. METHODS: The right sciatic nerve injury models were made using 60 adult Sprague Dawley rats. All models were randomly assigned into phosphate buffer saline (PBS) group, BMSC group and BDNF gene-modified BMSC group. 2 ?L BPS, 2 ?L BMSC solution and 2 ?L BDNF modified BMSC solution were separately transfered into injury site of each group. MAIN OUTCOME MEASURES: The retrograde horseradish peroxidase tracing was practiced to observe neural cell number in the Lumbar 4 and 5 spinal cord anterior horn at 2 and 4 weeks after microinjection. Sciatic nerve function index was used to observe rat motor function of injured limbs. Fluorescence excitation and immunohistochemistry were employed to detect BMSC survival and BDNF expression. RESULTS: Cell number was more, and nerve function recovery was better in the Lumbar 4 and 5 spinal cord anterior horn in the BDNF gene-modified BMSC group than in the PBS and BMSC groups. BMSC survival was found in the injured sites in the BMSC and BDNF gene-modified BMSC groups. BDNF expression was significantly more in the BDNF gene-modified BMSC group than in the BMSC group. CONCLUSION: BDNF gene-modified BMSCs have promotion effects on the recanalization and functional recovery of nerve fiber following peripheral nerve injury.

AIM: To construct lentiviral vector carrying the angiopoietin-1(Ang-1) gene,and make it express Ang-1 in the rat mesenchymal stem cells(rMSCs).METHODS: The cDNA encoding the CDS of Ang-1 gene was obtained from the placenta of the adult Fisher 344 rats with RT-PCR.After digestion with restrication endonuclease,the Ang-1 gene was recombined to construct the transfer plasmid PNL-Ang1-IRES2-EGFP.The three-plasmid system of lentiviral vector was consisted of PNL-Ang1-IRES2-EGFP,the packaging plasmid HELPER,and the envelope plasmid VSVG,which were co-transfected to 293T cells mediated by lipofectamin2000 to produce lentiviral particles.The rMSCs were infected by obtained lentiviral particles.The insertion of Ang-1 gene was detected by PCR,the mRNA expression of Ang-1 in rMSCs was detected with RT-PCR,the protein expression of Ang-1 was observed with immunocytochemistry and Western blotting methods.RESULTS: The result of sequencing showed that the cloned Ang-1 gene was consistent with the sequence reported in GenBank.After digestion with restrication endonuclease,the 1 512 bp fragment of Ang-1 gene and the 10.5 kb vector fragment of PNL-IRES2-EGFP were observed with gel electrophoresis.The insertion of Ang-1 gene in viral genome was confirmed.The EGFP expression was observed with the fluorescent microscope.In infected rMSCs,the mRNA and protein expressions of Ang-1 were confirmed.CONCLUSION: Lentiviral vector carrying Ang-1 gene has been successfully constructed.The infected rMSCs are able to express the Ang-1 mRNA and Ang-1 abundantly.This will facilitate the following exploratory development of Ang-1 gene-modified rMSCs.

ObjectiveTo investigate the changes of non-linear dynamics characteristic of electroencephalogram (EEG) under three states (eyes closed, mental arithmetic with eyes closed and orientation test with eyes closed), and the application of non-linear dynamic analysis for cognitive function research.MethodsEEG was recorded in 30 healthy young volunteers at three stages. The correlation dimension (D2) and approximate entropy (ApEn) were calculated for all subjects.ResultsD2 and ApEn increased significantly during mental tasks as compared to the rest state with eyes closed (P<0.01). In different mental tasks, the complexity of mental functions and the regions involved in the cognitive task were different.ConclusionNon-linear analysis methods are appropriate for the study of cognitive functions and can help to understand the working mechanism of brain during mental activities.