Objective To discuss the selection of conveyance and the temperature safeguards during the transport of blood specimens for centralized nucleic acid detection.Methods A total of five chips,which have been set every 10 minuets to record the temperature,have been placed in the Specimen box accordance with the appendix B ofblood transport requirements (WS/T 400-2012).Then,observe the temperature changes in case of ice been placed on both sides,sides and top,sides and bottom,sides and top,bottom of the specimen box respectively.Results In case of ice been placed on both sides of the specimen box,the temperatures were always higher than 10 ℃.In case of ice been placed on both sides and the top of the specimen box,the temperatures were all in range of 2-10 ℃ within 13 hours.In case of ice been placed on both sides and the bottom of the specimen box,only the temperatures of the top were always higher than 10℃.In case of ice been placed on both sides,top and bottom of the box,the temperatures of the bottom were always lower than 2 ℃.Conclusion In case of ice been placed on both sides and top of the box was the most appropriate temperature safeguards during the transport of blood specimens,while in the other cases,the temperatures were lower than 2 ℃,or higher than 10 ℃.

Objective To investigate the expression of FcγRⅡb on peripheral B cells and its clinical significance in primary Sj(o)gren's syndrome (pSS).Methods FcγRⅡb expression on peripheral B cells from 19 pSS patients and 15 healthy controls was examined by flow cytometry.The levels of serum anti-SSA and SSB antibodies were determined using enzyme-linked immunosorbent assay (ELISA).Data were analyzed with t test,one-way ANOVA,SNK-q test and Pearson's correlation test.Results The percentage of memory CD19+CD27+ B cell subpopulation was significantly lower in pSS patients [ (20.8±2.7)％] when compared to normal controls [(37.8±2.2)％ ](t=-4.002,P＜0.01).The level of expression of FcγRⅡb in active pSS memory CD19+CD27+ B cells [ MFI(74±8)] was significantly reduced when compared to inactive [ MFI( 132±11)] and normal controls [ MFI (139±12)] (F=10.699,P＜0.01).The level of expression of FcγRⅡb on memory CD19+CD27+ B cells from pSS patients was inversely correlated with Sj(o)gren's syndrome disease activity index (SSDAI) (r=-0.744,P=0.0003 ).pSS patients with the serum anti-SSA/SSB antibodies positive group [ MFI(75+3),(48±7)] displayed a lower expression of FcγRⅡb on memory CD19+CD27+ B cell than in patients with the serum anti-SSA/SSB antibodies negative group [MFI( 122±11),(108±9)] (t=-4.336 and -3.776 respectively,the P value of both tests were less than 0.01).The level of expression of FcγRⅡb in the memory CD19+CD27+ B cells of patients with active pSS was inversely correlated with anti-SSA antibody titers (r=-0.685,P=0.014).Conclusion The expression of FcγRⅡb on peripheral memory B cells from active pSS patients is inversely correlated with SSDAI and is also inversely associated with anti-SSA antibody levels.Decreased expression of FcγRⅡb might play an important role in the pathogenesis of pSS.

Objective To investigate the effects of the point-injection combined with neuromuscular facilitation rehabilitation techniques on post-stroke shoulder-hand syndrome. Methods A treatment group, 36 cases, was treated with the point-injection combined with neural facilitation of rehabilitation techniques. And a control group, 30 cases, was treated with massage therapy. Observed the clinical manifestations and used Fugl-Meyer to assess the joint activities, pain degree and motion function of upper limbs before and after therapy. Results Compared with the control group, the treatment group showed better improvement of joint activity scope and degree, and alleviation of pain (P<0.05). Conclusion Point injection and neuromuscular rehabilitation treatment has a better effect ain treating sequelare of brain stroke and it is worth applying.

AIM: To study the effects of exogenous metallothionein (MT) and ZnCl 2-induced MT production on biological action of homocysteine(HCY)in vascular fibroblasts METHODS: -TdR, -Pro incorporation and LDH leakage were measured, the cellular viabilities were calculated by trypan blue exclusion test and the intracellular contents of MT were assayed by [ 109 Cd]-hemoglobin saturation method in cultured rat vascular fibroblasts. RESULTS: Proliferation, collagen production of vascular fibroblasts in HCY-treated group were significantly increased compared with control group in a concentration-depedant manner. HCY (500 ?mol/L) increased LDH leakage and decreased the cellular viabilities ( P

ObjectiveTo investigate the effect of IκB kinase (IKK-β) on migration and proliferation of bone marrow mesenchymal stem cells(BMSCs) from patients with systemic lupus erythematosus (SLE).MethodsHuman bone marrow aspirates were collected from iliac of six donors and six SLE patients and cultured in vitro.Migration of BMSCs were observed by wound healing and transwell migration assays.Proliferation of BMSCs was quantified by cell counting kit-8 assay.Total RNA was extracted and reverse transcribed into complementary DNA.Real-time PCR technique was used to determine the gene expression of IKK-β at transcription level.The expression of IKK-β and phospho-IKK-β(p-IKK-β) protein were determined by Western blotting analysis.Statistical analysis was conducted with or Mann-Whitney rank test.Results① The migration rate of BMSCs from SLE patients(5.2±3.8)‰ were significantly reduced as compared with normal controls (7.0±2.9)‰(P＜0.05 ).The proliferation of BMSCs of SLE patients (0.21±0.49)was lower than that from healthy controls ( 1.00±0.35 )(P＜0.05 ).②) No difference in IKK-β3 mRNA expression between SLE ( 1.9± 1.4) subjects and normal controls (1.9±2.4) (P＞0.05).IKK-β protein expression of BMSCs from SLE patients (1.41 ±0.19) increased significantly compared with healthy controls (0.93±1.24) (P＜0.05).③ Inhibitor of IKK-β caused a significant increase in cell migration (3.3±1.6)‰ and proliferation (1.13±0.26) of BMSCs from SLE patients compared with untreated cells (2.3±1.1)‰ and (0.81±0.17),respectively (P＜0.05).ConclusionMigration and proliferation of BMSCs are significantly decreased in SLE patients.IKK-β may be involved in migration and proliferation of BMSCs.

Objective To investigate the status of exercise autonomy of chronic obstructive pulmonary disease (COPD) patients at stationary phase in community and to analyze the influencing factors of exercise autonomy so as to guide the external hospital control of COPD. Methods Totals of 124 COPD patients at stationary phase from three target communities in the area of the First Affiliated Hospital of Zhengzhou University were investigated by the method of convenience sampling. We analyzed the status of exercise among them. Non-conditional Logistic stepwise regression was used to determine the influencing factors of exercise autonomy. Results A total of 122 valid questionaires were collected. Among 122 patient, 51 (41.80%) patients never carried out, 33 (27.05%) occasionally carried out and 38 (31.15%) persisted in respiratory function exercise. Besides, 44 (36.07%) patients never carried out, 36 (29.51%) occasionally carried out and 42 (34.43%) persisted in all-round exercise. There were 53 patients with the good exercise autonomy. The frequent hospitalization was the independent protective factor of patients' exercise autonomy (P＜ 0.05). Conclusions COPD patients at stationary phase in community have poor exercise autonomy. Patients with more frequent hospitalization tend to carry out exercise more actively.

Objective? To explore the effects of grading health education model on self-care behavior and health behavior among elderly bronchiectasis patients outside hospital. Methods? From March 2015 to March 2017, we selected 120 elderly bronchiectasis patients at the First Affiliated Hospital of Zhengzhou University by convenience sampling. All of the patients were divided into control group and observation group with the method of the random number table, 60 cases in each group. Control group carried out routine health education. Observation group received the grading health education model dividing health education into three stages and pushing education content by WeChat;a total of 12 times of health education were implemented. The interventional cycle was three months between two groups. The Appraisal Self-Care Agency Scale-Reviewed (ASAS-R-C) and the Self-Rated Abilities for Health Practices Scale (SRAHP) were used to assess the self-care behavior and health behavior of patients outside hospital. Results? Before intervention, there were no statistical differences in the score of ASAS-R-C and SRAHP (P＞ 0.05). After intervention, the dimension scores of ASAS-R-C, SRAHP and the total score were higher than those before intervention with statistical differences (P＜0.05). After intervention, the scores of self-care goal, self-care skill, the total score of ASAS-R-C, health responsibility, sport management and the total score of SRAHP of intervention group were (25.62±2.18), (24.52±2.41), (74.72±6.27), (24.78±2.75), (25.21±2.16) and (98.35±8.16) higher than those of control group with statistical differences (P＜ 0.05). Conclusions? Grading health education model is propitious for elderly bronchiectasis patients to clear the nursing goal, improve nursing skills, enhance their health responsibility and improve their sport management abilities which is worthy of popularization and application.

Objective:To explore the application effect of early warning model of chemotherapy complications in nursing care of non-small cell lung cancer during chemotherapy.Methods:By using convenient sampling method, 106 patients with non-small cell lung cancer treated by chemotherapy in the Thoracic Surgery Department of the First Affiliated Hospital of Zhengzhou University from January 2016 to February 2019 were selected as the study objects. According to the time of admission, they were divided into the control group ( n=52) and the observation group ( n=54) . The control group was given routine nursing care during chemotherapy, while the observation group was given nursing care through early warning model of chemotherapy complications during chemotherapy. Hamilton Anxiety Scale (HAMA) , Hamilton Depression Scale (HAMD) and European Organization for the Research and Treatment of Cancer QLQ-C30 (EORTCQLQ-C30) were used to compare the intervention effect of the two groups, and the incidence of complications during chemotherapy was counted. Results:The total incidence of complications was 26.92% (14/52) in the observation group and 46.15% (24/52) in the control group, the difference was statistically significant (χ 2=4.147, P<0.05) . Three months after the intervention, HAMA (9.87±4.25) and HAMD (15.54±3.58) in the observation group were lower than those in the control group; the differences were statistically significant ( t=4.738, 8.296; P<0.05) . The functional score, symptom score, overall quality of life score and single measurement item score of the observation group were higher than those of the control group with statistical differences ( P<0.05) . Conclusions:During chemotherapy for non-small cell lung cancer, nursing through the early warning model of chemotherapy complications can reduce the incidence of chemotherapy complications, alleviate the negative emotions of patients, and improve the quality of life, which is worthy of clinical application.

Objective:To understand the incidence of HIV infection, high risk behaviors and pre-exposure prophylaxis/post-exposure prophylaxis (PrEP/PEP) utilization in men who have sex with men (MSM) in Beijing.Methods:Sample size was estimated to be 1 227 persons and 207 person year respectively in the survey and the cohort by using Epi Info 7.0 software. Using convenient sampling method, MSM were recruited by using Wechat app. Questionnaire was completed online to collect the information about demographic characteristics, high risk behavior, and utilization of PrEP/PEP of the MSM. MSM collected dry blood spot (DBS) samples by themselves, and mailed the DBS samples to laboratory for HIV nucleic acid testing. Open cohort was established and those with HIV negative nucleic acid testing results were followed up. Non-conditional binary logistic regression method was used to identify the associated factors for high risk anal sex in the last month and having multiple homosexual partners in the last month.Results:A total of 1 147 MSM were recruited, and follow up for 236 person years was conducted in 956 MSM with negative HIV nucleic acid testing results. The detection rate of new HIV infection was 1.3 per 100 person-years (3/236). During the last month, the proportions of consistent condom use in anal sex and oral sex were 50.7% (238/469) and 4.9% (23/469). In the MSM, 5.9% (43/723) had sex with HIV positive partners in the last month. 9.8% (103/1 049) used PrEP, and 8.7% (91/1 049) used PEP. The proportion of consistent condom use in PrEP and PEP were 34.3% (24/70) and 72.2% (39/54) respectively. Logistic regression analysis revealed that compared with those who used no PrEP/PEP, those who used PrEP/PEP were more likely to have unprotected anal sex in the last month (a OR=3.16, 95% CI:1.45-7.18), and more likely to have multiple homosexual partners in the last month (a OR=2.64, 95% CI:1.19-6.30), and compared with those who used no Rush Popper or drugs in the last month, those who used Rush Popper or drugs in the last month were more likely to have unprotected anal sex in the last month (a OR=2.34, 95% CI:1.67-3.30), and more likely to have multiple homosexual partners (a OR=2.42,95% CI:1.76-3.33). Conclusions:It is necessary to strengthen the health education to promote condom use and introduce the harm of drug use in MSM. In PrEP and PEP services, it is still necessary to suggest consistent condom use for MSM.

Objective To investigate the effects of dezocine on the first flatus time with patients undergoing gynecological laparoscopic surgery.Methods A total of 116 patients undergoing gynecological laparoscopic surgery were included from January 2015 to June 2015 in our hospital and randomly divided into control group and dezocine group. The patients in control group were given routine care; meanwhile, the patients in dezocine group were given postoperative intramuscular injection of dezocine 7.5 mg immediately after they were back to the ward. Visual Analogue Scale (VAS) was used to evaluate the scores at 2 h (T1), 4 h (T2), 6 h (T3), 12 h (T4) and 24 h (T5) after the operation. The first flatus time was recorded.Results The VAS scores of T1, T2 and T3 in dezocine group were significantly lower than those in control group (P<0.01). There were no significant differences in the VAS scores of T4 and T5 between two groups, since the drug concentration decreased due to the metabolism over time. The first flatus time in dezocine group was shorter than that in control group, with a statistically significant difference (P<0.01).Conclusions Intramuscular injection of dezocine 7.5 mg after gynecological laparoscopic surgery can effectively relieve the postoperative pain and shorten the first flatus time.