Objective: To establish the method of fingerprint analysis of leaves of Panax Notoginseng by HPLC。 Methods: Zorbax C 18 (4.6?250mm) column was used, CH 3OH 4% H 3PO 4 solution (65∶35) as mobile phase and detection wavelength at 203nm, and ginsenosides Rb 3 was used as reference compound. Results: Fingerprint consisted of 12 common peaks. Conclusion: This method is accurate, reliable and provides a scientific basis for controlling the quality of leaves of Panax Notoginseng.

Objective:To establish the quality standard for Chidan Ganxian granules. Methods:TLC was used to identify Paeoni-ae Radix Rubra, Rhizoma Polygoni Cuspidati, Radix Salviae Miltiorrhizae, Herba Saururi and Radix et Rhizoma Rhei in Chidan Ganx-ian granules, and the content of paeoniflorin was determined by HPLC. The stationary phase was an Apollo C18 column ( 150 mm × 4. 6 mm, 5 μm), the mobile phase was acetonitrile-water(12. 5∶87. 5), the flow rate was 1. 0 ml·min-1, the detection wavelength was at 230 nm, and the temperature was 30℃. Results:The characteristic spots of the granules were the same as those of the standard samples without any interference from the negative control. Paeoniflorin had a good linear relationship within the range of 0. 120-1. 436 μg(r=0. 999 4), and the average recovery was 99. 8% with RSD of 1. 80%(n=6). Conclusion:The method is simple, ac-curate, reliable and reproducible, which can be used in the quality control of Chidan Ganxian granules.

Objective:To establish an HPLC-ELSD method for the determination of mahuannin A in ephedrae radix et rhizoma. Methods:The content of mahuannin A was determined by an HPLC-ELSD method on an Alltima TM C18 column (250 mm × 4. 6 mm, 5 μm). The mobile phase was acetonitrile-water (28∶ 72) with a flow rate of 0. 7 ml·min-1, and the column temperature was 30℃. The temperature of drift tube heater was 105℃ and the flow rate of carrier gas was 2. 8 L·min-1 . Results:The linear range of mahua-nnin A was 42. 56-383. 04 μg·ml-1(r=0. 999 8). The average recovery and RSD was 99. 9% and 1. 96%(n=6), respectively. Conclusion:The method is simple and the result is accurate. It can be used for the quality control of ephedrae radix et rhizaoma.

Objective:To optimize the extraction process of the total flavonoids from Ephedrae Radix Et Rhizoma. Methods:The purification method of the total flavonoids from Ephedrae Radix Et Rhizoma was optimized with the yield and content of the total fla-vonoids as the indices. Based on the above research, the process parameters were optimized by an orthogonal test. Results:The opti-mum purification conditions were as follows:the volume fraction of ethanol was 50%, the stirring extraction time was 20 min, and the liquid-solid ratio was 8∶ 1(ml·g-1). Conclusion:The optimum purification technology is simple and reproducible, and suitable for the industrial production.

Objective:To optimize the extraction process for the total saponins from the root of Thladiantha dubia Bunge. Meth-ods:The extraction technology was optimized by orthogonal experiment with the dissolution content of total saponins as the index and the extraction times, extraction duration and ratio of solid to liquid as the influencing factors. Results:The optimum extraction condi-tions for the total saponins from the root of Thladiantha dubia Bunge were as follows:the reflux extraction was conducted twice(1. 5 h per time) with 70% ethanol as the solvent, and the ratio of solid to liquid was 1 ∶6. Conclusion:The optimum extraction technology is simple, reproducible and stable.

Objective:To study the preventive and therapeutic effects of Jiechangyan Qixiao granule ( JQX) in the rats with ulcer-ative colitis ( UC) induced by dextran sulfate sodium .Methods:The UC model was induced by drinking dextran sulfate sodium ( DSS, 4%) freely in Wistar rats weighting 180-220g.Guben Yichang tablets and sulfasalazine was used as the standard drugs for the compari -son.After 7-day intragastric administration of Jiechangyan Qixiao granules at the dose of 2.70, 1.35 and 0.68 g· kg-1 · d-1 , the se-rum levels of malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), interleukin -6(IL-6)and tumor necrosis factor-α(TNF-α), and the protein expression of myeloperoxidase (MPO) and intercellular adhesion molecule (ICAM-1)in colon and nuclear factor(NF-kBp65) were detected.Results:Compared with the model group, the granule at high and medium dose could sig-nificantly increase the activity of SOD in blood and decrease the contents of MDA , TNF-αand IL-6 (P<0.05 or P<0.01).The granule could also notably decrease the MOP activity in colonic mucous of UC rats (P<0.05 or P<0.01), and the contents of NF-kBp65 and ICAM-1 in the inflammation reaction(P<0.01).Conclusion: JQX shows promising efficacy in the treatment of UC rats induced by dextran sulfate sodium .

Ephedrae Radix Et Rhizoma is a traditional Chinese medicine, and the effect of antiperspirant has been recorded in books on Chinese medicines in all dynasties. By referring the relative literatures on Ephedrae Radix Et Rhizoma from home and abroad, the study progress in the chemical constituents, pharmacological actions and clinical application of Ephedrae Radix Et Rhizoma in the latest 40 years were reviewed to lay foundation for the reasonable exploitation and utilization of Ephedrae Radix Et Rhizoma.

Thladiantha dubia Bunge is a traditional Chinese medicine in Manzu region applied in the treatment of pain in waist and leg, or strain in lumbar without adverse reaction. By referring to the relative literatures on Thladiantha dubia Bunge from home and abroad, the study progress in the chemical constituents and pharmacological actions of Thladiantha dubia Bunge in the recent 30 years were reviewed to lay foundation for the reasonable exploitation and utilization of Thladiantha dubia Bunge.

Objective:To investigate the therapeutic effects of Gengnian Anshu granule on the endocrine system of menopausal rats. Methods:The rats aged 13-15 months received intragastrical treatment with Gengnian Anshu granule at the doses of 1. 9,0. 95 and 0. 48 g·kg - 1 ·d - 1 for 6 weeks. At the end of treatment,serum and hypothalamic hormone levels were detected,and the organ indices including uterus and ovaries were determined. Results:In the menopausal rats,Gengnian Anshu granule could significantly in-crease the serum estradiol(E2 )(P < 0. 05 or P < 0. 01),decrease serum follicle-stimulating hormone(FSH)and luteotrophic hor-mone(LH)(P < 0. 05 or P < 0. 01),increase norepinephrine(NE),dopamine(DA),5-hydroxytryptamine(5-HT)and β-endor-phine(β-EP)(P < 0. 05),increase the number of cells positive for estrogen receptor(ER)protein expression in hypothalamus(P <0. 05 or P < 0. 01),and decrease the number of cells positive for gonadotropin-releasing hormone(GnRH)protein expression(P <0. 01). Conclusion:With a multi-target action mechanism and an integral regulatory role,Gengnian Anshu granule can provide favor-able regulation against endocrine disorder in menopausal rats.