Livin is the inhibitor of apoptosis proteins (IAPs), a new member, in most malignant tissues of high expression, and its main function is to inhibit cell apoptosis. Apoptosis and tumor cells is closely related to the occurrence and development, more on which the survival of tumor angiogenesis. Vascular endothelial growth factor (VEGF) is one of the strongest of vascular growth factor, and almost all human tumors and tumor cell lines have over-expression of it. Livin and VEGF protein expression in tumor was summarized.

Objective To study the expression and clinical significance of BFGF factor(BFGF) and basic fibroblast growth factor receptor(BFGFR) in papillary thyroid carcinoma (PTC).Methods 80 casesof PTC and 10 cases of nodular goiter underwent surgical treatment from Mar.2001 to May.2005 in Shengjing Hospital of China Medical University.Samples were collected and immunohistochemistry staining was performed to detect the expression of BFGF and BFGFR.Results The expression rate of BFGF and BFGFR was 72.5％ (58/80)vs 70.0％ (56/80) in PTC,and 0.0％ (0/10) vs 0.0％ (0/10) in nodular goiter.The difference had statistical significance(P ＜0.05).The expression level of BFGF and BFGFR in PTC had no relation with sex,age or tumor size(P ＞0.05),but has relation with lymph node metastasis and UICC stage(P ＜0.05).The expression of BFGF was positively correlated with the expression of BFGFR (r =0.330,P =0.003).Conclusions BFGF and BFGFR are highly expressed in PTC but have no expression in nodular goiter or normal thyroid tissues.The expression of BFGF and BFGFR in PTC is closely related to lymph node metastasis and UICC stage,indicating the 2 may be involved in tumorigenesis,invasion,and metastasis.

Objective To investigate intraoperative neural monitoring(IONM)for prevention of recurrent laryngeal nerve injury(RLN)during thyroid cancer operation.Methods 288 cases of thyroid cancer admitted from Jun.2011 to Jun.2013 in our hospital were studied and they were divided into the observation group(140 cases) and the control group(148 cases) according to whether IONM was used during surgery.The incidence of RLN injury was compared between the 2 groups.Results The injury rate of RLN was lower in the observation group(0.67％) than in the control group(3.57％)and the operation time is shorter than the latter(P ＜0.001).There was no statistical difference for the operative bleeding volume and the average hospitalization time between the 2 groups (P ＞ 0.05).Conclusion INOM can help to shorten the operation time and reduce the incidence of RLN injury.

Objective To investigate the role of ?-catenin gene in breast tumorigenesis.Methods Mutation and expression of ?-catenin gene in 42 breast cancer tissues were detected by polymerase chain reaction-single strand conformation polymorphism and immunohistochemistry.Results The rate of abnormal expression of ?-catenin in breast cancer tissuse was 59.5%(25/42).Mutation of ?-catenin gene was not detected in breast cancer tissue.Conclusions Abnormal expression of ?-catenin plays an important role in human breast tumorigenesis,but the cause of abnormal expression of ?-catenin is not mutation of ?-catenin gene.

Objective To investigate the expression and relationship of livin and mutant p53 in hepatocellular carcinoma (HCC). Methods The expression of livin and mutant p53 were evaluated using the SP immunohistochemistry in 80 HCC tissue, 39 hepatic cirrhosis tissue, 33 normal tissue beside the hemangiomas of liver. Results The integrated A average results showed that the intension of positive expression of livin aligned by turns was HCC tissue (Median=5.09; P25～P75=3.06～8.28), hepatic cirrhosistissue(Median=3.05; P25～P75=2.49～4.25), normal liver tissue(Median=1.99; P25～P75=1.54～2.54) (P<0.001), respectively. It also showed that the intension of positive expression of p53 in HCC tissue (Median=43.13; P25～P75=20.41～78.53) was higher than that in hepatic cirrhosis tissue (Median=20.30;P25～P75=14.90～28.08), as well as in that of normal liver tissue (Median=15.52;P25～P75=12.81～21.80) (P <0.001), but it made no sense in statistics between hepatic cirrhosis tissue and normal tissue of liver. The expression of livin was obviously correlated with p53 in HCC tissue(r=0.241, P<0.05). Conclusion The overexpressian of livin and p53 and their positive correlation showed that livin may play a crucial role in the origin and development of HCC in coordination with p53.

Objective To analyze the mutations of SLC12A1 gene in nine Chinese families with Bartter syndrome type I (BS1),and analyze the relationship between genotype and phenotype.Methods The next generation sequencing was used to detect mutations in nine BS1 patients including eight with antenatal BS (aBS) and one with classical BS (cBS).Clinical characteristics and biochemical findings at the first admission as well as follow-up were reviewed.Results 15 different mutations of SLC12A1 gene were identified,including 11 novel ones.Among nine probands,seven were compound heterozygotes,two were homozygotes.All patients presented with polydipsia and polyuria,and eight with growth retardation.All patients had lower than-normal serum chloride concentration,metabolic alkalosis,and elevated basal renin activity and aldosterone,and seven had hypokalemia.Through treatment of indomethacin and/or potassium chloride,biochemical indicators could roughly restored normal.Conclusion These findings will enrich the human gene mutation database (HGMD) and provide valuable references to the genetic counseling and diagnosis for Chinese population.

Objective: To investigate the influencing factors of the implementation of precision medical services based on clinician cognition, and provide a scientific reference for the implementation and advancement of precision medical services. Methods: Using electronic questionnaires, clinicians from 48 hospitals in 12 provinces(autonomous regions and municipalities directly under the Central Government) were surveyed from July to September 2019, with 341 valid questionnaires collected. Descriptive statistics and factor analysis were performed on the survey data. Results: Three dimensions that affect precision medical services were identified from the recovered questionnaires, namely external support factors, hospital drive capabilities, patient information provision and protection. The contribution rate of variance after orthogonal rotation was 35.157%, 22.234% and 16.343% respectively. Conclusions The external supporting factors should be developed, the hospital driving ability should be strengthened, and the patient information reception and privacy information security should be ensured to provide an important basis and guarantee for the implementation of precision medical service.

OBJECTIVE To establish an HPLC fingerprint of Xiao’er resuqing oral liquid， and to determine the contents of twelve index components. METHODS HPLC method was adopted. The determination was performed on Venusil MP C18 column with mobile phase consisting of acetonitrile-0.1% phosphate aqueous solution （gradient elution） at a flow rate of 1.0 mL/min. The detection wavelength was set at 210 nm， the column temperature was 30 ℃， the injection volume was 10 μL. HPLC fingerprint of Xiao’er resuqing oral liquid was established by using the Similarity Evaluation System of Chromatographic Fingerprint of TCM （2012 edition） to evaluate the similarity. The contents of 12 components were determined， including （R， S）-goitrin， 3，5-O-dicaffeoyl quinic acid， puerarin， forsythin， forsythoside A， chlorogenic acid， baicalin， saikosaponins d， wogonoside， baicalein， emodin and chrysophanol. RESULTS The similarity of HPLC fingerprints of 13 batches of Xiao’er resuqing oral liquid was greater than 0.97， and 14 common peaks were confirmed. The contents of the above 12 index components in 13 batches of Xiao’er resuqing oral liquid were as follows： 0.078-0.172， 1.564-2.736， 1.338-2.578， 0.426-0.872， 1.477-2.628， 1.396-2.447， 4.052-9.146， 0.367- 0.692， 1.974-4.674， 1.274-2.969， 0.085-0.167 and 0.155-0.307 mg/mL. CONCLUSIONS The established HPLC fingerprint and content determination methods have high accuracy and high specificity， which can be used for the quality evaluation of Xiao’er resuqing oral liquid.