Objective:To explore the timing and indications of preventive antibiotics-using cholecystectomy.Methods:304 cases of cholecystectomied patients were divided into 4 groups and compared.Results:Compare wound infection rate of preventive group with that of control group,χ2=8.5,P<0.01,with that of group having more infectious chance,χ2=7.1,P<0.01,with that of group having less infectious chance,χ2=0.4,P>0.05.Compare wound infection rate of all patients that have different preventive medicine-using time,χ2=0.02,P>0.05.Conclusion:preventive antibiotics-using in group having more infectious chance can decrease postoperative wound infection rate apparently.Medicine should be used from anesthesia induction period,and using time shouldn't excess 2 days.Of all medicines,gentamicin and metronidazole are preferable medicines to choose.

Objective To observe the biological behavior of chondrocytes in chitosan based hydrogel in vitro and to evaluate the possibility of chitosan and glycerophosphate mixed gel using as injectable scaffolds in tissue engineering.Methods A novel injectable thermosetting gel was made of chitosan and glycerophosphate mixture.Chondrocytes isolated by enzymic digestion from the articular cartilage of the young rabbits aged 3-4 weeks were cultured in monolayer system.After primary culture and subculturing,the cells were seeded in chitosan based gel in concentration of 5?10~7/ml in vitro.Characteristics of chondrocytes during 4-week culture were observed with invert microscope.MTT was used to detect the seed growth after one-week culture.In 4th week,after haematoxylin eosine and toluidine blue staining,the artifical cartilage was performed type Ⅱ collagen immunohistochemistry and observed by scanning electron microscope.Results Chondrocytes retained their round morphology,multiplied,and produced matrix in chitosan and glycerophosphate mixture gels.The results of immunohistochemistry were strongly positive.Conclusion That most chondrocytes appeared to occupy large areas of rough chitosan based gels,guarantees a suitable environment where chondrocytes secrete matrix macromolecules and preserve their round morphology in vitro.Chitosan and glycerophosphate mixture gel may be a vehicle for injectable tissue-engineered cartilage.

Curcumin, which is extracted from the plant Curcuma longa, has been used in the therapeutic arsenal for clinical oncology. Curcumin has chemopreventive and antitumoral activities against some aggressive and recurrent cancers. The expressions and activities of various proteins, such as inflammatory cytokines and enzymes, transcription factors, and gene-products linked with cell survivals and proliferation, can be modified by curcumin. Moreover, curcumin decreases the toxic effect of mitomycin C. Though curcumin has shown highly cytotoxic to some cancer cell lines, curcumin is insoluble and instable in water. The solubility of curcumin could be enhanced by utilizing the solubilizing properties of rubusoside. In addition, the selective delivery of synthetic analogs or nanotechnology-based formulations of curcumin to tumors may improve the chemopreventive and chemotherapeutic effects. The focus of this short review is to describe how curcumin participates in antitumor processes in breast cancer cells.
Breast ; Breast Neoplasms ; Cell Line ; Curcuma ; Curcumin ; Cytokines ; Diterpenes, Kaurane ; Glucosides ; Imidazoles ; Medical Oncology ; Mitomycin ; Molecular Mechanisms of Pharmacological Action ; Niacin ; Plants ; Proteins ; Solubility ; Transcription Factors ; Water