1.Surveyor assay to diagnose persistent Müllerian duct syndrome in Miniature Schnauzers.
Young June KIM ; Hyuk Jin KWON ; Hyuk Soo BYUN ; Donguk YEOM ; Jea Hong CHOI ; Joong Hyun KIM ; Hosup SHIM
Journal of Veterinary Science 2017;18(4):547-549
Persistent Müllerian duct syndrome (PMDS) is a pseudohermaphroditism in males characterized by the presence of Müllerian duct derivatives. As PMDS dogs often lack clinical symptoms, a molecular diagnosis is essential to identify the syndrome in these animals. In this study, a new molecular method using DNA mismatch-specific Surveyor nuclease was developed. The Surveyor nuclease assay identified the AMHR2 mutation that produced PMDS in a Miniature Schnauzer as accurately as that obtained by using the conventional method based on restriction digestion. As an alternative to the current molecular diagnostic method, the new method may result in increased accuracy when detecting PMDS.
Animals
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Diagnosis
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Digestion
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Disorders of Sex Development
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DNA
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Dogs
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Humans
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Male
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Methods
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Pathology, Molecular