The experimental rats were fed with 5 test diets of different protein quality.RNA content and 3-methylhistidhie(3-MH) in urine were determined.The results showed that the RNA content of the skeletl muscle cells was significantly different in rais fed with different diet.A linear correlation between RNA content and amino acid score of food protein was perceived.Food with the higher protein quality,the more 3-MH in urine was excreted.A good correlation between excrstiou of 3-MH and Net Portein Utilization(NPU),an index reflecting protein quality,existed too.These suggested that protein quality regulates the balance between auabolism and catabolism,meaning the turnover of skeletal muscle proteinThe mechanism of strengthening anabolism,not reducing catabolism,can explain that high protein quality could improve growth of animals.

AIM To investigate the mechanism of anti-tumor effects of curcumin on human SPC-A1 cells. METHODS Cell culture、fluorescence microscope、TUNEL method and irradiation immunity techniques were used. RESULTS ①By the action of curcumin, SPC-A1 cells grew against the wall and then peeled off gradually;fluorescence microscope showed the nucleus broke into pieces, and cell split into apoptosis bodies in different sizes; The TUNEL method further certified that apoptotic cells reached 42 67% at the concentration of 20 ?mol?L -1 curcumin at 24 h. ②The level of intracellular cAMP concentration in the SPC-A1 cells,treated with curcumin, was significantly higher than that of the control group. ③Treated with 20 ?mol?L -1 curcumin for 24 h, the expression of Caspase-8 mRNA of human SPC-A1 cells was up-regulated. CONCLUSION Curcumin can induce the apoptosis of SPC-A1 cells, and one of its molecular mechanism may be associated with the rising of the level of intracellular cAMP concentration, and up-regulating expression of Caspase-8.

Objective To investigate the changes of serum procalcitonin (PCT) level in patients with severe trauma,and to analyze its relationship with multiple organ failure (MODS).Methods From October 2013 to October 2018,the serum PCT levels were measured in 40 patients with severe trauma complicated with MODS (MODS group:40 cases) and 62 patients without MODS (non-MODS group:62 cases) in Yuncheng Central Hospital.The relationship between PCT and severity of trauma score (ISS),sequential organ failure (SOFA),acute physiology and chronic health status score (APACHE Ⅱ) and prognosis was analyzed.The predictive value of PCT in the prognosis of patients with MODS and severe trauma combined with MODS was analyzed by ROC.Results The serum PCT levels of the MODS group were (8.25 ± 2.35) μg/L,(7.26 ± 2.49) μg/L,(3.62 ± 1.75) μg/L on the 1 st,3rd and 7th day after admission,respectively,which were higher than those of the control group (t =7.561,9.561,8.251,all P ＜0.05).The serum PCT level in the MODS group increased with the increase of APACHE Ⅱ score and ISS score(F =12.108,9.173,all P ＜ 0.05).The serum PCT level in the death group was higher than that in the survival group[(11.82 ± 3.75) μg/L vs.(4.92 ± 1.26) μg/L],and the difference was statistically significant (t =7.959,P ＜0.05).The area under MODS curve predicted by PCT for severe trauma patients was 0.848,with sensitivity and specificity of 80.00％ (32/40),82.50％ (33/40),respectively.The area under MODS curve predicted by PCT was 0.747,with sensitivity and specificity of 77.50％ (31/40),72.50％ (29/40),respectively.Conclusion The serum PCT level in patients with severe trauma complicated with MODS is significantly higher than that in patients without MODS,and its level is closely related to trauma,disease progression and prognosis.It is of great value to predict the occurrence and prognosis of MODS.

Objective To observe the ingrowth characteristics of the interface between hydroxyapatite coated intervertebral implants and vertebral cortex loaded with physiological compressive stress. Methods Twelve titanium alloy intervertebral implants special for macaque were prepared, 4 of which were coated with corundum (control group) and 8 of which were coated with hydroxyapatite (HA, observed group). One control and two observed implants were randomly inserted into the intervertebral spaces of L2,3, L3,4 and L4,5 in each of 4 healthy homogenous adult macaques (2 males and 2 females). Roentgenology was performed at 3, 6, 12, 24 and 40 weeks postoperatively. Histomorphometry as well as histology were also evaluated at 40 weeks postoperatively. Results All the animals recovered well from the operation. Three days after operation all the animals began to stand and walk with normal gait. Gross anatomy showed excellent healing in the annulus fibrous involved, and no implant loosening or migration was found. On 40 weeks postoperatively, the interface of the observed group were filled with calcified mature bone and partly-mineralized osteoid tissue, the content of calcified bone as well as the amount of osteoblasts and osteocytes were significantly higher in observed group, and the differences were of statistical significance (t=5.001, P=0.000 and t=16.983, P=0.000). A tight connection was observed between the vertebral bones and HA coating. The thickness of coating decreased form 130-150 ?m to 100-130 ?m. And no evident breakage or debris was found on the implant coating. Conclusion HA coating was stable in vivo and had favorable biocompatibility with vertebral bone. It was more effective than corundum coating in inducing intervertebral cortical bone ingrowth under physical compressive loading.

Objective To study the criteria and technique of transsphenoidal microsurgery on pituitary adenoma invaded cavernous sinus Methods 42 patients with pituitary adenoma underwent 45 transsphenoidal operations Sellar base was opened as large as possible under C arm X ray fluoroscopy or neuronavigation monition Dura matter was cut open under microscope Tumors were removed through the direction and tunnel they in invade Bromocriptine was administered to those with little remains Partial remains and those have no response to bromocriptine were given radiotherapy Results No modality 21 tumors were nearly totally removed, 18 tumors were subtotally resected, 3 were partially resected MRI scanned in 2～3 months after the operation showed that 19 tumors disappeared, 20 tumors had little residul, 3 tumors partially remained Conclusion Pituitary adenomas invading the cavernous sinus with enlarged sella turcica and shown soft in MRI can be submitted to transsphenoidal operation Microsurgical technique can ease tumor removal and protection of the sellar and internal carotid artery

Objective To explore ways for expanding the now-limited coagulation volume of percutaneous microwave coagulation therapy(PMCT) in liver in order to enhance the complete necrosis rate of large hepatic tumors with obviously fewer punctures. Methods Fresh pig livers in vitro and rabbit, pig livers in vivo with and without blood supply were coagulated using the self-designed HM-WIA microwave coagulator to make clear antenna length, microwave power, coagulation time and blood flow influence the microwave volume(MCV).Results The device enlarged the MCV by 3 times using single electrode with an antenna of 36 mm in length along with microwave power of 45-55 W and coagulation time of 15-19 min,the short axis of the coagulation area was 5.6 cm in vitro, 5.3 cm in vivo with no blood supply.The antenna 36 mm in length was proved the best in keeping the finest coagulation shape.The MCV was 2.8 times as large without blood supply as with it (P

The significance of the blood glutathion reductase activity coeff i-.cient (BGR-AC) as the guide line to assess riboflavin status in 28 radar operators was studied by comparing with the amount of their urinary excreted riboflavin.The 28 subjects were randomized into 4 groups and the riboflavin intake was 0.53.mg,1.0 mg,2.0 mg,and 3.0 mg respectively.It was found that there was a significant negative correlation between BGR-AC and urinary excretion of riboflavin,and with the incrase of riboflavin intake,there was a significant decrease of BGR-AC in Groups Ⅰ,Ⅱ,and Ⅲ though the difference between Group Ⅲ and Group Ⅳ was insigificant.On the basis of the BGR-AC values of the 4 groups,an exponential equation was established which tells the riboflavin requirement of radar operators.It is defined that when BGR-AC is smaller than equal to 1.24,it is normal and when it is larger than 1.24,it means abnormal riboflavin status of the individual.

The pleomorphic adenoma of epiglottis is rare. When the tumor grows larger, some patients may have symptoms of pharyngeal foreign body sensation, but other patients have no obvious symptom. The tumor is mainly found through indirect laryngoscopy or electronic laryngoscopy. This disease can be confirmed by pathological diagnosis after tumor resection.
Adenoma, Pleomorphic ; Adult ; Epiglottis ; Humans ; Laryngeal Neoplasms ; Male

Objective: To construct the recombinant eukaryotic expression plasmid pHsp70-hsv-tk(pHT)for further target gene therapy research.Methods: The oligomers of primers were synthesized based on the reported sequences of hsv-tk and with the pHSV-106 plasmid as the template.Then the hsv-tk gene fragment was amplified and inserted into the pD3SX vector.E.coli DH5? was transformed with the recombinant plasmids and the bacterial colonies containing the recombinant plasmids were identified by the PCR cloning method,and the recombinant plasmids were extracted and purified.Results: The recombinant eukaryotic expression plasmid pHT was obtained.The target gene obtained by PCR amplification had the same molecular size as predicted.The recombined pD3SX plasmid contained correct recombinant hsv-tk sequences.Conclusion: The plasmid pHT was successfully constructed,which can be used for further target gene therapy research.