Objective To study the amount of rhizospheric microorganisms and soil enzyme activity influenced by Paridis Rhizoma in different locations and of different strains. Methods The amount of rhizospheric microorganisms, soil enzyme activity and their correlation were researched through field survey and collection of rhizospheric soil in Paridis Rhizoma cultivated in Three Gorges Reservoir Region and by microbial dilution plate culture method. Results The amount of rhizospheric microorganisms in Paridis Rhizoma from different habitats showed significant differences. The dominant species in soil microflora was bacteria; the second one was actinomycetes; the fewest one was fungus. The variation trend of the amount of rhizospheric microorganisms was not consistent with the variation trend of rhizospheric microorganisms diversity index. The activity of soil phosphatase, invertase and pepsin in Paridis Rhizoma from different habitats varied. The correlation analysis showed that the correlation between the soil enzyme activity and the amount of rhizospheric microorganisms existed. Conclusion Choosing the suitable strains and habitats of Paridis Rhizoma is beneficial to enhancing the amount of rhizospheric microorganisms and soil enzyme activity, which can create good micro-ecological environment for growth and cultivation of Paridis Rhizoma.

This study was aimed to examine the expression of apoptosis-associated gene Fas in HeLa cell, explore the effects of the co-immobilized cytokines (tumor necrosis factor-alpha and interferon-gamma), and probe the potential mechanism of action. The preparation and application of the research couple IFN-gamma and TNF-alpha to the polystyrene cell culture plate were performed using the Photo-immobilization method, with different doses (20 ng/well and 200 ng/well) and synthesized optical active material. HeLa cells were treated with cytokines for two dose and 1, 3, 6 days. The result showed that the free cytokines induced HeLa apoptosis quickly, yet the HeLa apoptosis induced by co-immobilized cytokines had longer effect.
Apoptosis ; drug effects ; genetics ; Drug Synergism ; HeLa Cells ; Humans ; Immobilized Proteins ; chemistry ; pharmacology ; Interferon-gamma ; chemistry ; pharmacology ; Tumor Necrosis Factor-alpha ; chemistry ; pharmacology ; Up-Regulation ; fas Receptor ; metabolism

BACKGROUND: The proliferation of peripheral blood stem cells among peripheral blood mononuclear cells (PBMCs) invitro remains unclear. There is no optimal marker for tracing PBMCs transplanted in vivo.OBJECTIVE: To observe the degree of PBMC proliferation in stem cell medium by EdU labeling and to explore thefeasibility of EdU-labeled peripheral blood stem cells.METHODS: New Zealand rabbit PBMCs were isolated and cultured for 1 to 5 days in stem cell medium supplementedwith EdU. The cells were observed and counted at 0, 1, 2, 3, 4 and 5 days in culture. The cells were harvested at eachtime point and stained with EdU fluorescent reagents. Then, confocal microscopy and flow cytometry were used to detectEdU-labeled cells.RESULTS AND CONCLUSION: (1) Freshly isolated rabbit PBMCs were rounded and showed clear outline. After 1 dayculture, most of the cells were suspended in the medium, spherical or round. There were also a few cell clusters andadherent cells scattered in a triangle or polygon shape; after 2 days culture, more cell debris were observed, and mostcells were round; when cultured for 3-5 days, increased cell debris, smaller cell mass and decreased cell densitysignificantly were observed. (2) With the prolongation of culture time, the cell count decreased gradually. (3) Whencultured for 1 day, EdU labeled cells in red were scattered. The number of cells marked with EdU red label increasedsignificantly at day 2 and remained unchanged after 3 days of culture. At 5 days of culture, the number of red cellsmarkedly decreased; the highest positive rate of EdU-labeled cells was (2.38±0.10)% at 2 days after culture. To conclude,these results showed that the proportion of proliferating cells in rabbit PBMCs was very low. EdU is capable of labelingproliferative cells among PBMCs.

Objective:To explore the effects of micro-class combined with closed-loop modularized teaching in practical course of emergency nursing.Methods:From March to July 2018, this study selected nursing students of four classes in Grade 2016 of Xianyang Vocational Technical College as subjects by convenience sampling. All of nursing students were randomly divided into observation group ( n=160) and control group ( n=162) with the method of tossing a coin by classes. Control group carried out the routine teaching, while observation group applied the micro-class combined with closed-loop modularized teaching in practical course of emergency nursing. This study compared the nursing students' examination scores and recognition on teaching model between two groups. Results:At the end of practical course of emergency nursing, the theoretical and operation achievements of observation group were (85.59±7.24) and (88.34±5.62) respectively higher than those of control group with statistical differences ( t=-6.308, -7.008; P<0.05) . Recognition of observation group on teaching model was higher than that of control group also with a statistical difference ( P<0.05) . Conclusions:Micro-class combined with closed-loop modularized teaching can improve the learning effects of nursing students in practical course of emergency nursing and is recognized highly by nursing students which is worthy of generalization and application.

Objective:To explore the application of nursing of theory education combined with experience education in the rehabilitation of patients with idiopathic pulmonary artery hypertension (IPAH) .Methods:Nursing of theory education combined with experience education was carried out in Department of Respiratory Medicine in Affiliated Hospital of Jining Medical University in December 2017. Using the convenient sampling method, 53 patients with IPAH who were admitted from August 2016 to December 2017 were selected as the control group and they were given routine nursing. A total of 53 IPAH patients who were admitted from January 2018 to May 2019 were selected as the research group, and they were given nursing of theory combined with experience education at basis of the control group. Before the intervention and 1 month after discharge from the hospital, patients in two groups were assesses by Exercise of Self-Care Agency (ESCA) scale, the MOS 36-item Short-Form Health Survey (SF-36) , 6 minutes walking distance (6MWD) and Borg Scale (BS) , and the occurrence of complications in the two groups during hospitalization and discharge for 1 month was recorded.Results:After 1 month of discharge, scores of self-care skills, self-care concept, self-care responsibilities and health knowledge and the total self-care score of ESCA were respectively (35.52±2.16) , (22.32±1.55) , (27.15±2.02) , (48.67±2.85) and (130.67±3.38) . Scores of physical functioning, role-physical, vitality, role-emotional, social functioning and mental health and overall health score of SF-36 were respectively (74.73±12.83) , (71.79±11.31) , (72.06±11.43) , (69.67±10.54) , (71.23±10.72) , (72.43±13.30) and (65.45±14.27) . The 6MWD distance was (512.67±86.85) m, and BS score was (3.79±0.85) . Compared with the control group, the differences were statistically significant ( P<0.01) . During hospitalization and one month after discharge, the complication rate of the research group was lower than that of the control group, and the difference was statistically significant ( P<0.05) . Conclusions:Nursing of theory education combined with experience education can improve the self-care ability of IPAH patients, improve their quality of life, and reduce the incidence of complications, which is worthy of clinical application.

Objective:To construction the early ambulation scheme for patients after laparoscopic colorectal cancer surgery.Methods:Based on evidence-based nursing method combined with clinical practice, the draft of early ambulation scheme for patients after laparoscopic colorectal cancer surgery was prepared. From August to October 2022, two rounds of correspondence were conducted with 15 experts by Delphi method, and the final draft of early ambulation scheme for patients after laparoscopic colorectal cancer surgery was formed.Results:The coefficient of expert positivity in the two rounds of correspondence was 100%, the coefficient of authority was respectively 0.86 and 0.87, and the Kendall cofficient of concordance was 0.241 and 0.167 ( P<0.001) . The final scheme consisted of preface and text. The text of the scheme contained 2 first-level items, 10 second-level items and 42 third-level items. Conclusions:The early ambulation scheme for patients after laparoscopic colorectal cancer surgery in this study is scientific and feasible, providing references for clinical medical staff to guide early getting out of bed activities for patients after laparoscopic colorectal cancer surgery.

Objective:To construct advanced practice competencies for nurses in tumor prevention and early screening and promote nurses' professional role in cancer prevention and treatment.Methods:A literature review was conducted to draft advanced nursing practice competencies in tumor prevention and early screening. A first-round expert consultation questionnaire was developed, followed by two rounds of Delphi expert consultations involving 25 experts from 10 hospitals and institutions nationwide engaged in clinical nursing, nursing management, clinical medicine, genetics, and epidemiological research in tumor prevention and screening.Results:Advanced practice competencies for nurses in tumor prevention and early screening were formulated around seven aspects: communication, health promotion and education counseling, clinical practice, professional development, ethical decision-making, evidence-based practice and research, and leadership.Conclusions:Core competencies necessary for nurses to engage in advanced practice in tumor prevention and early screening were established, thus providing a basis for enhancing their role in cancer care.

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide and macrophage polarization plays an important role in its pathogenesis. However, which molecule regulates macrophage polarization in NAFLD remains unclear. Herein, we showed NAFLD mice exhibited increased 17β-hydroxysteroid dehydrogenase type 7 (17β-HSD7) expression in hepatic macrophages concomitantly with elevated M1 polarization. Single-cell RNA sequencing on hepatic non-parenchymal cells isolated from wild-type littermates and macrophage-17β-HSD7 knockout mice fed with high fat diet (HFD) for 6 weeks revealed that lipid metabolism pathways were notably changed. Furthermore, 17β-HSD7 deficiency in macrophages attenuated HFD-induced hepatic steatosis, insulin resistance and liver injury. Mechanistically, 17β-HSD7 triggered NLRP3 inflammasome activation by increasing free cholesterol content, thereby promoting M1 polarization of macrophages and the secretion of pro-inflammatory cytokines. In addition, to help demonstrate that 17β-HSD7 is a potential drug target for NAFLD, fenretinide was screened out from an FDA-approved drug library based on its 17β-HSD7 dehydrogenase inhibitory activity. Fenretinide dose-dependently abrogated macrophage polarization and pro-inflammatory cytokines production, and subsequently inhibited fat deposition in hepatocytes co-cultured with macrophages. In conclusion, our findings suggest that blockade of 17β-HSD7 signaling by fenretinide would be a drug repurposing strategy for NAFLD treatment.