1.Empirical study of titration of IgG antibody against blood group in pregnant women by using domestic microcolumn gel Coombs'test
Lingbo LI ; Dongqian WANG ; Yunsheng CHEN ; Ning LI ; Weijia CHEN
Chinese Journal of Immunology 1999;0(12):-
Objective:To compare microcolumn gel Coombs test and test tube Coombs test for IgG anti-A and anti-B titre in serum of pregnant women with blood type O.The critical titre for IgG anti-A and anti-B should be established in domestic microcolumn gel Coombs' test.Methods:524 blood samples of pregnant women with blood type O,whose husbands were of blood type A or B,were detected simultaneously by domestic microcolumn gel Coombs' test and test tube Coombs test.The results were analyzed using paired "t " test,"?2 " test and regression analysis.Results:IgG anti-A mean titres determined by the two methods separately were 249.98 and 120.85,and IgG anti-B mean titre were 156.98 and 76.38.IgG anti-A and anti-B titration in domestic microcolumn gel Coombs'test showed significantly higher titres(mean 2.07 fold and mean 2.06 fold) than in test tube Coombs test in all samples studied(t=19.64,P
2.Analysis of inhaled and food allergen spectrum of patient with allergic rhinitis in north of Zhejiang.
Yongcai WENG ; Dongsheng GU ; Dongqian CENG ; Yuwu CHEN ; Li ZHANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(14):1028-1030
OBJECTIVE:
To explore the inhaled and food allergen distribution of patient with allergic rhinitis in north of Zhejiang and to analyze the difference of allergen distribution among different age groups.
METHOD:
One thousand and forty eight patients in north of Zhejiang diagnosed with allergic rhinitis in our outpatient department were tested with skin prick test (SPT). The positive rate of inhaled and food allergens were calculated. To analysis the difference of positive rate between children and adult.
RESULT:
Nine hundred and eighty-eight cases (94.3%) had the positive reaction. Dermato phagoides farinae and Dermatophagodies pteronyssinus had the highest positive ratio (72.1%, 71.8%) in inhalation group, followed cockroach (14.1%). In food group, Shrimp and peanuts had the highest positive ratio (18.3%,14.2%). Between children group and adults group, positive rate of food allergen was significantly different (P < 0.05), but of inhaled allergen was not significantly different.
CONCLUSION
The distribution of some allergens in children group and adults group was variable. The study shows that Dust mite was the mostly responsible common allergen in north of Zhejiang.
Adolescent
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Adult
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Aged
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Air Pollutants
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immunology
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Allergens
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immunology
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Child
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Child, Preschool
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Female
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Food
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Humans
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Male
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Middle Aged
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Rhinitis, Allergic
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immunology
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Skin Tests
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Young Adult
3.Effect of nursing of Traditional Chinese Medicine on cognitive function among aged people with ;mild cognitive impairment in elderly care institution
Dongqian LI ; Jianping SUN ; Zhilan YANG ; Guifang NIU ; Dan SONG ; Dandan CHENG
Chinese Journal of Practical Nursing 2016;32(24):1855-1859
Objective To explore the effect of nursing of Traditional Chinese Medicine on cognitive function among aged people with mild cognitive impairment in elderly care institution. Methods A total of 66 cases with mild cognitive impairment elderly that previously were screened out from two pension institutions in Taiyuan city as the research objects. According to the endowment institutions where the objects of study dwelled was divided into control group (34 cases) and intervention group (32 cases). The intervention group accepted nursing of Traditional Chinese Medicine plan for four months. The control group received the disease related knowledge health education. The cognitive function of the two groups were assessed using the Montreal Cognitive Scale (MoCA) before and after the intervention. Results Before the intervention, two groups had no statistical significance in each dimension score and total score of MoCA. After the intervention, four dimensions scores including ability of visual spatial and execute , attention , delayed recall , directional force and the total score of the intervention group respectively were (2.67±1.09) points, (4.07±1.08) points, (1.97±0.81) points, (4.83±0.99) points, (18.80±1.74) points while the control group were (2.15 ± 0.71) points, (3.30 ± 0.63) points, (1.39 ± 0.97) points, (4.24 ± 1.41) points, (15.06 ± 2.98) points, and the difference was statistically significant (t=1.91-6.06, P < 0.05 or 0.01) ;Before intervention the language score, delayed recall score and the total score of the intervention group were (1.43±0.77) points, (1.57±1.10) points, (17.27±1.99) points, respectively while after the intervention were (1.80 ± 0.66) points, (1.97 ± 0.81) points, (18.80 ± 1.74) points, there was statistically significant difference (t =-2.16,-2.11,- 4.34, P < 0.05 or 0.01). Conclusions Nursing of Traditional Chinese Medicine can delay the process of cognitive decline of mild cognitive impairment in the elderly, and have a certain effect on prevention and treatment of patients with mild cognitive impairment.
4.The current situation investigation on oral care of the tube intubation in the emergency department of the 3A hospital in Shanxi province
Hongyan ZAN ; Dongqian LI ; Xiaomei TAN ; Meirong SHA ; Xianping XIE
Chinese Journal of Practical Nursing 2017;33(34):2688-2690
Objective To investigate the status quo of oral nursing in the emergency department of top three hospitals in Shanxi province, and to analyze the existing problems so as to provide the basis and reference for improving the clinical operation standard. Methods In the form of WeChat questionnaire, a questionnaire survey was conducted to investigate 190 nurses who met the requirements in emergency departments of 6 top three hospitals in Shanxi province. Results All the 6 hospitals surveyed did not incorporate oral care into the nurse examination program; 28.42%(73/190) of the subjects had oral assessments of endotracheal intubation according to their clinical experience;chlorhexidine (70.00%,133/190) and physiological saline (65.79%,125/190) were the most commonly used oral care solutions in clinic;48.42%(92/190) of nurses performed this operation for 6-10 min;91.58%(114/190) of nurses believed that oral care played an important role in the recovery of these patients. Conclusions The oral care and oral care of the patients in the emergency department are mostly clinical experience, so it is urgent to establish a unified standard of implementation on the basis of evidence. The professional knowledge of oral nursing of the patients undergoing oral trachea intubation is low, so it is suggested that the training of emergency nurses be strengthened and the operation should be included in the assessment of nursing operations.
5.Low concentration contrast medium and low radiation dose for infants and young children abdominal CT scanning
Haoyan LI ; Jihang SUN ; Xiaomin DUAN ; Yong LIU ; Dongqian WANG ; Ling JIANG ; Qifeng ZHANG ; Tong YU ; Yun PENG
Chinese Journal of Interventional Imaging and Therapy 2018;15(4):243-246
Objective To assess the feasibility of low concentration contrast medium (270 mgI/ml) and low radiation dose (100 kV) for enhanced CT scanning in infants and young children abdominal CT examination.Methods Ninety children with abdomen tumors or abdominal injuries who underwent contrast-enhanced CT examination were selected.The patients were divided into 3 groups (each n= 30):Group A with tube voltage of 120 kV for non-contrast enhanced and parenchymal phase scanning and iodixanol contrast-medium (320 mgI/ml);group B with tube voltage of 100 kV for non-contrast enhanced and parenchyrnal phase scanning and iodixanol contrast-medium (270 mgI/rnl);group C with tube voltage of 100 kV for non-contrast enhanced and parenchymal phase scanning and iodixanol contrast-medium (270 mgI/ml).The 4-point scale was used to evaluate the quality of parenchymal phase imaging.The standard difference (SD) of CT value in subcutaneous fat,SNR and CNR of liver parenchyma,splenic parenchyma,renal cortical,renal vein,and abdominal aorta were measured at parenchymal phase,and CT dose index of volume (CTDI,ol),dose length product (DLP) and effective dose (ED) were recorded.The data were statistically analyzed among 3 groups.Results There was no significant difference of SNR,CNR nor objective scores of liver parenchyma,splenic parenchyma,renal cortical,renal vein and abdominal aorta among 3 groups (all P>0.05).The differences of CTDIvol,DLP and ED among 3 groups were statistically significant (all P<0.01).The CTDIvol had no statistical difference between group B and group C (P = 0.001,0.002),DLP (P = 0.013,0.004) and ED (P = 0.03,<0.001) of group A had statistical difference with those of group B and C.Conclusion CNR of the abdominal image can be guaranteed using low concentration contrast medium (270 mgI/ml) combined with 100 kV tube voltage for CT scanning of infants and young children,therefore satisfying clinical diagnostic requirements.
6.Analysis of the compliance and effectiveness of the allergen specific immunotherapy in patients with allergic rhinitis
Yong Cai WENG ; Dongsheng GU ; Dongqian ZENG ; Li ZHANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2016;30(7):542-545
Objective:To investigate the effectiveness and compliance of the 2 year specific immunotherapy in patients with allergic rhinitis. Method:Two hundred and sixty-two patients of allergic rhinitis were treated with SLIT for 2 years. The symptom questionnaire about effectiveness and compliance were obtained 2 years after SLIT. The patient satisfaction was also investigated. Result:Sixty patients had complete compliance and 202 patients had poor compliance. Of the 142 children, 40 (28%) cases had complete compliance, and of the 118 adult patients, 20 cases (17%) had complete compliance. There was obvious difference between the two (P<0.05). Comparing of before and after treatment, total medication score, total nasal symptom score and every symptom score decreased obviously (P<0.05). Two years after treatment, the total effective rate was 63%,there was significant difference between the children group and the adult group (P<0.05). Conclusion:There are a lot of factors affecting the treatment compliance of sublingual immunotherapy, such as patients, family, health care workers and social support, etc. In the course of treatment, a series of strategies can be adopted to improve the treatment compliance. SLIT is an effective method for the treatment of allergic rhinitis, and the treatment effect of children is better.
7.Optimization of water extraction process of Maxing kechuan granules
Dongqian LIANG ; Jingjing WANG ; Xin WANG ; Tianchuan CHAI ; Chunhua LI
China Pharmacy 2023;34(17):2095-2100
OBJECTIVE To optimize the water extraction process of Maxing kechuan granules. METHODS With the contents of ephedrine hydrochloride, bergenin, prim-O-glucosylcimifugin, 5-O-methylvisamin, naringin and hesperidin and the rate of extraction as the evaluation indexes, the weight was determined by the analytic hierarchy process(APH)-entropy weight method, and the comprehensive score was calculated as the response value. Based on the single-factor test, the Box-Behnken response surface method was used to investigate the factors, and the best water extraction process of Maxing kechuan granules was optimized; process validation was also carried out. RESULTS The best water extraction process of Maxing kechuan granules optimized was as follows: soaking for 40 minutes, adding 8 times water, and extracting for 180 minutes. After three validation tests, the comprehensive score was 94.82 (RSD=0.96%, n=3), which had a small difference from the predicted value of 94.64. CONCLUSIONS The water extraction process of Maxing kechuan granules is stable and reliable, which can provide a reference for the development of the preparation.
8.Prevalence and molecular characterization of Cryptosporidium in captive-bred Mustela putorius furo in Jiangsu Province
Jinyang ZHANG ; Yonghua ZHOU ; Jie GUO ; Jiajia LI ; Yanyan WU ; Zhenghai ZHOU ; Haiyun ZHU ; Xinyu LUO ; Dongqian CHEN ; Qiaoqiao LI ; Xinchao LIU ; Wenchao LI
Chinese Journal of Schistosomiasis Control 2023;35(1):73-77
Objective To investigate the prevalence and molecular features of Cryptosporidium in captive-bred Mustela putorius furo in Jiangsu Province.. Methods A total of 290 fresh stool samples were collected from a ferret farm in Jiangsu Province on May 2017, and the small subunit rRNA (SSU rRNA) gene of Cryptosporidium was amplified in stool samples using nested PCR assay. The actin, cowp and gp60 genes were amplified in positive samples and sequenced to characterize Cryptosporidium species/genotypes. Results A total of 18 stool samples were tested positive for Cryptosporidium SSU rRNA gene, with a detection rate of 6.2%. Sequence and phylogenetic analyses of SSU rRNA, actin and cowp genes characterized Cryptosporidium isolated from captive-bred ferrets as Cryptosporidium sp. ferret genotype. In addition, gp60 gene was amplified in 10 out of 18 stool samples tested positive for Cryptosporidium. Conclusions Cryptosporidium is widely prevalent in captive-bred ferrets in Jiangsu Province, and Cryptosporidium sp. ferret genotype is the only Cryptosporidium genotype in ferrets.
9.Targeted inhibition of GRK2 kinase domain by CP-25 to reverse fibroblast-like synoviocytes dysfunction and improve collagen-induced arthritis in rats.
Chenchen HAN ; Yifan LI ; Yuwen ZHANG ; Yang WANG ; Dongqian CUI ; Tingting LUO ; Yu ZHANG ; Qian LIU ; Hao LI ; Chun WANG ; Dexiang XU ; Yang MA ; Wei WEI
Acta Pharmaceutica Sinica B 2021;11(7):1835-1852
Rheumatoid arthritis (RA) is an autoimmune disease and is mainly characterized by abnormal proliferation of fibroblast-like synoviocytes (FLS). The up-regulated cellular membrane expression of G protein coupled receptor kinase 2 (GRK2) of FLS plays a critical role in RA progression, the increase of GRK2 translocation activity promotes dysfunctional prostaglandin E4 receptor (EP4) signaling and FLS abnormal proliferation. Recently, although our group found that paeoniflorin-6'-