OBJECTIVE:To establish the quality standard of Kouyanqing chewable tablet.METHODS: Flos Lonicerae Japonicae, Radix Scrophulariae and Radix Et Rhizoma Glycyrrhizae in the tablet were identified by TLC. The content of Chlorogenic acid was determined by HPLC. The chromatographic separation was performed on Hypersil ODS column (150 mm?4.6 mm,5 ?m) with mobile phase consistd of methanol-0.8%phosphoric acid solution(19∶81) with a flow rate of 0.8 mL?min-1. The detection wavelength was set at 327 nm. RESULTS: The TLC spots were clear and concentrated. There was a good linear relationship for Chlorogenic acid within the range of 11.3～101.7 ?g?mL-1(r=0.999 1). The average recovery was 99.04%(RSD=1.31%,n=9). CONCLUSION: This method is sensitive, accurate, reproducible and specific, and it can be used for the quality control of Kouyanqing chewable tablet.