Objective: To establish the assay methods for paeoniflorin and baicalin in Jianweishu Granules (Radix Scutellariae, Radix Paeoniae Alba, Radix Astragali seu Hedysari, Radix Notoginseng, etc.). Methods: Radix Astragali, Radix Notoginseng; Radix Paeoniae Alba and Herba Dendrobii in Jianweishu Granule were identified by TLC. The paeoniflorin and baicalin in Jianweishu Granule were determined by HPLC on shim pack CLC ODS C 18 Column (6.0mm?150mm,10?m); using methanol water phosphoric acid (47∶53∶0.2) and methanol water (30∶70) as mobile phases, respectively; the flow rate were at 1.0 mL?min -1 ; the detection wavelength were 280nm and 230nm, respectively; the column temperature were at room temperature and 40?C respectively. Results: The average recoveries are 98.94% and 98.67%. RSD are 2.31% ( n =5) and 1.36% ( n =5). Conclusion: The methods are simple, sensitive, accurate and reproducible.

Purpose To explore the ultrasonic features of tibial nerve (TN) in tarsal tunnel of type 2 diabetes mellitus patients with diabetic peripheral neuropathy (DPN), and to provide the basis for assessment and treatment. Materials and Methods Fifty-four patients with type 2 diabetes mellitus were divided into the DPN group including 31 patients with diabetic periphreal neuropathy and the non-DPN group including 23 patients without diabetic periphreal neuropathy, 22 cases of non diabetic patients were also enrolled as the control group, all the subjects underwent medial tibial nerve ultrasound examination, and the ultrasonic features of tibial nerve (including morphological parameters:left-right diameter, anterior-posterior diameter, thickness sectional area and color Doppler lfow signals) were compared. Results Left-right diameter and anterior-posterior diameter of DPN group were larger than those of non-DPN group and the control group (P<0.01). Ultrasonic features of tibial nerve of DPN group included nerve bundle hypoechoic, vaguedscreen clothstructure, thickening and fuzzy of neural membrane, and the unclear boundaries to adjacent tissue. There was no statistically signiifcant difference in color Doppler signal of TN (P>0.05) among the DPN group, non-DPN group and the control group. Conclusion There are some characteristics features of tibial nerve in tarsal tunnel of type 2 diabetes mellitus patients with DPN, ultrasound can be used to evaluate the morphology of TN in type 2 diabetes mellitus patients with DPN and to provide morphological basis for evaluation and treatment selection.

Objective To explore the clinical effect of small cell lung cancer(SCLC) treatment with recombinant human endostatin injection and cis-platinum complexes.Methods 100 patients with SCLC were divided into 2groups.The experiment group used cis-platinum complexes while the control group used recombinant human endostatin injection.Results The effective rate of experiment group was obviously higher than the control group ( 74.0％ vs60.0％,x2=2.16,P ＜ 0.05 ).The median survival time of experiment group was better than control group( t=2.02,P ＜ 0.05 ).The diverse of untoward effect of two groups had no difference ( P ＞ 0.05).Conclusion Recombinant human endostatin injection and cis-platinum complexes were better therapy for SCLC,which had stable effect,no severe untoward effect and overcome the drug-fast of cis-platinum complexes.

[Objective]To study the properties of xenogeneic deproteinized bone (DPB) used as scaffold in the bone tissue engineering and its application to the spinal fusion of the lumbar intertransverse process in a goat. [Methods]The deproteinized bone was derived from an adult pig femoral cancellous bone through physical and chemical treatments.The cell-material complex was observed under the inverted phase contrast microscope to evaluate the adhesion and the growth of the osteoblasts. The experimental model of the spinal fusion in the lumbar intertransverse process was produced in 24 male goats aged 6-8 months, which were divided into 3 groups according to different implant graft. All the samples were harvested at 4,8,12 weeks postoperatively, and a series of examinations were performed, including the radiography and the histomorphological assay.[Results]It showed that The DPB maintained natural pore network system, it hardly had any antigen, so it had good histocompatibility. In the spinal fusion model of lumbar intertransverse process,the cell-material complex could form cartilage and had a new bone formation in a multipoint way, the osteogenic process was almost the same as the auto-ilium osteogenesis and had a good mechanical strength.[Conclusion]The xenogeneic deproteinized bone is a good material in the bone tissue engineering, which can be used as an osteogenesis scaffold and provides a stable fusion.

Objective To establish a method for determining the contents of chrysophanol, emodin and sodium Danshensu in Shen An Kang Capsules(SAKC). Methods Chrysophanol and emodin contents were determined on Lichrospher100 C18 column (4.6 mm? 250 mm, 5 ? m) with the mobile phase of Methanol- 0.1 % Perchloric acid (85 ∶ 15) at a flow rate of 1 mL? min-1, and the detection wavelength was 254 nm. Sodium Danshensu content was determined on Kromasil column C18( 4.6 mm? 250 mm, 5 ? m) with the mobile phase of actonenitirile - 1.2 % Glacial acetic acid(8 ∶ 92)at a flow rate of 0.5 mL? min-1, detection wavelength being 280 nm. Results The linearity of emodin was obtained in the range of 0.020 16～ 0.100 80 ? g(r=0.999 6), linearity of chrysophanol obtained in the range of 0.034 56～ 0.172 80 ? g(r=0.999 9)and linearity of sodium Danshensu obtained in the range of 0.144 8～ 0.868 8 ? g(r=0.999 6).The average recovery was 97.70 % with RSD 2.38 % for emodin, 97.69 % with RSD 1.92 % for chrysophanol and 97.52 % with RSD 0.77 % for sodium Danshensu. Conclusion The method is accurate, reproducible , and is helpful for the quality control of Shen An Kang Capsules.

BACKGROUND:Heterogeneous deproteinized bone because of wide source and its special biological characteristics maybe a good bone tissue engineering scaffold.Its immunogenicity and mechanical properties are different by different interventions.OBJECTIVE:To proof the preparation technology,immunological and mechanical properties of heterogeneous deproteinized bone.DESIGN,TIME AND SETTING:A control observational experiment was performed in the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy from February to October 2008.MATERIALS:Fresh femoral cancellous bones extracted from adult pigs,which were not including cartilages and cortical bones,were prepared into size of 3 cm?0.5 cm?0.5 cm.Their long axis direction was the same with orientation of trabeculae.The apparent pore densities of the used bones were almost same.METHODS:The bones were soaked in chloroform-carbinol(1:1) 24 hours for degrease.They were shaken and washed by distilled water at 50 ℃,and then put into 20% H2O2 for 24 hours.The procedure repeated three times.The bones were soaked in distilled water at room temperature for 24 hours,and then dryed.MAIN OUTCOME MEASURES:The gross morphological and histological features of the deproteinized cancellous bones were observed.The contents of amino acid and their biomechanical properties were measured.RESULTS:The three-dimensional space structures of the deproteinized cancellous bones were not damage greatly,and they had a natural pore network system which composited of hydroxyapatite and collagen.The contents of collagen amino acids in the deproteinized cancellous bones had no obvious difference from fresh cancellous bones,but the wave crests of aromatic amino acids such as tyrosine and methionine were disappear.The elastic moduli of deproteinized cancellous bones were significantly higher than those of fresh cancellous bones(P

Objective A HPLC method was established for the determination of paeonol and paeoniflolin in cortex moutan from different processing.To investigate the necessity of processing to the cortex moutan.Methods The HPLC(high performance liquid chromatogram)method was established for the determination of the contents of the paeonol and paeoniflolin in cortex moutan.The conditions of the chromatograph method was as following:AC18 column was used.The mobile phase for paeonol determination was methanol-water(45:55)and detection wavelength was 274nm.The mobile phase for paeoniflorin determ ination was acetonitrile-0.1%H3PO4(15:85)and detection wavelength was 230nm.Results The results showd that the linear ranges of paeonol and paeoniflorin determ ination were 0.36～2.2?g/L～and 0.042～1.06?g/L respectively.The average recoveries of paeonol and paeoniflorin were 96.30%(RSD=0.97%)and 98.21%(RSD=0.64%)respectively.Conclusion The method is simple,accurate and reproducibility.The experimental results showd that the contents of paeonol and paeoniflorin in coaex moutan from diferent processing were positive correlated,while the contents of cortex moutan from Sichuan were higher than that of the others.We had better combine the pharmacodynamic action study to illuminate the feasibility of processing to the Cortex Moutan.

Through reviewing the concept, work content and standard work process of anesthesia clinic, this article analyzed the moral value of anesthesia clinic:beneficial to use the medical resources rationally, to improve patient safety, to enhance humanistic care, and to reflect the value of the anesthesia doctor. Then, it discussed the realization way of anesthesia clinic and aimed to improve the safety of operation and anesthesia, at the same time, to enhance the humanistic care of patients.

AIM:To establish the quality control for Finger-citron Formula Granule. METHODS: The TLC,HPLC and fingerprint chromatogram were used to make quality standard. RESULTS: The TLC spots were clear and 5,7-dimethoxy-coumarin showed a good linearity at a range of 0.46-4.14 ?g.The average recovery was(99.01%)(n=6) with RSD 1.58%. CONCLUSION: The method could be used as the quality control of Finger-citron Formula Granule.

Objective: To determinate the content of isoimperatorin in Exocarpium citri grandis from 10 various species and origins. Methods: The isoimperatorin was determinated at 305nm by TLC Scanning. Results: The content of isoimperatorin in Exocarpium citri grandis was very low, and the law of distribution could not be found. Conclusion: Although the content of isoimperatorin in Exocarpium citri grandis was very low, the method is very sensitive and simple and the result is accurate.