This review will discuss aerosol device technologies available for pulmonary vaccine deliveries.The possibilities of adopting aerosol-generation for the purpose of pulmonary immunization are described.Aerosol-generation systems might offer advantages in respect to vaccine stability and antigenicity.The noninvasive,relatively safe and low-cost net of pulmonary delivery may provide great benefits to the public health vaccination campaign.

Objective To summarize the clinical features of brucellosis and investigate the experience of diagnosis and treatment of the disease .Methods Data from epidemic reports and clinical characteristics from 172 ca-ses of patients with brucellosis were retrospectively analyzed .Results 130 out of 172 cases of were diagnosed as bru-cellosisaise.Of the misdiagnosed 42 cases,13 cases were misdiagnosed as lumbar spine ,10 cases as upper respiratory tract infection 8 cases as pancytopenia,5 cases as orchitis epididymitis,2 cases as hepatitis,1 case as prostatitis, 1 case as cervical disease ,1 case as myocarditis ,1 case as rheumatoid arthritis .And after treatment ,142 cases recov-ered completely,16 cases improved.The total efficiency was 100.0%.2 cases without standard treatment recurred ,an interval of 3 to 15 months.Conclusion So the clinical manifestation of brucellosis is lack of specificity that misdiag-nosis rate is high;The manipulation combined with standard antibiotic therapy may have certain effects on early -stage brucellosis .

ObjectiveTo investigate the value of Color Doppler Flowing Imaging in the diagnosis of female breast diseases,analysis of the clinical characteristics of breast disease.Methods The data of 451 patients with breast disease which diagnosed by Color Doppler Flowing Imaging were retrospectively analyzed,the statistical results and clinical treatment were analyzed finally.Results In all cases,the top three were breast hyperplasia,breast fibroids,and breast cancer,accounting for 56.1％,10.6％ and 5.3％ ;breast hyperplasia was the highest incidence in all ages,and it showed an increasing trend before the age of 50,gradually decreased after 50 years old; the breast adenofibroma was the highest incidence in the 30 years of age and gradually decreased with increasing age;breast cancer frequently occurring in women after the age of 60,but the deterioration tendency often appears in the age 40 to 50.ConclusionTo diagnose the breast disease by Color Doppler Flowing Imaging,the doctor should combine with breast disease characteristics of the disease,as well as patient age,medical history and other factors on the results of a comprehensive diagnosis.

Objective To observe the effect of Bushen Yiqi Hexue Recipe(Recipe to reinforce kidney,replenish qi and harmonize blood)on the endometrial gland apoptosis in blastocyst implantation dysfunctional mice.Methods The mice models of blastocyst implantation dysfunction were made and divided into control,model and treated groups with 30 in each.Pregnancy rate,number of implantation blastocysts,the expression of proliferating cell nuclear antigen(PCNA),bax,bcl-2 and activated Caspase-3 were observed.Results Compared with the control group,pregnancy rate and mean implanted blastocysts in the model group were remarkably reduced.Compared with the model group,pregnancy rate and mean implanted blastocysts in the treated group were significantly improved(P

Objective: To study clarifying for Naoqing Oral Liquid (Radix Puerariae, Radix Astragali, etc.) with chitin instead of ethanol. Methods: By studying precipitation effect of two clarification methods, in the meantime, studying the influence of different concentration of chitin on the clarification effect of Naoqing Oral Liquid. Results: Chitin had the more effective components than ethanol, and assured the stability of the preparation and shortened production period of the preparation. Conclusion: Chitin can clarify Naoqing Oral Liquid instead of ethanol.

OBJECTIVE:To evaluate the methodological bias of systematic review and the reliability of counclusions of tradi-tional Chinese medicine (TCM) combined with transarterial chemoembolization in the treatment of primary liver cancer,and pro-vide evidence-based reference. METHODS:Retrieved from Cochrane Library,PubMed,Guideline,CBM,CJFD and Wanfang Da-tabase,high quality systematic review about TCM combined with transarterial chemoembolization in the treatment of primary liver cancer was collected for reevaluation. RESULTS:Totally 12 systematic review were involved. Results showed TCM combined with transarterial chemoembolization can improve tumor’s objective response,prolong the survival time of patients,improve life quali-ty,reduce postoperative adverse reactions with no serious adverse reactions reported related to TCM in the treatment of primary liv-er cancer. CONCLUSIONS:The clinical efficacy of TCM combined with transarterial chemoembolization is superior to transarterial chemoembolization alone in the treatment of primary liver cancer,with better safety.

Objective:To observe the influence of long-term exercise training on carotid intima-media thickness (IMT)in patients with mild-to-moderate hypertension.Methods:A total of 92 patients with mild-to-moderate es-sential hypertension (EH)were randomly and equally divided into exercise group and routine treatment group.Exer-cise group received exercise training based on routine treatment.All patients were followed up one year.Influence of long-term exercise training on carotid artery diameter and carotid IMT was observed in EH patients.Results:Af-ter one year,there were significant reductions in systolic blood pressure (SBP),diastolic blood pressure (DBP),ca-rotid artery diameter and carotid IMT in both groups,P<0.05 all;compared with routine treatment group,there were significant reductions in SBP [(145.72±11.31)mmHg vs.(130.89±13.01)mmHg],DBP [(88.49±7.32) mmHg vs.(81.71±8.45)mmHg],carotid artery diameter [(6.34±1.23)mm vs.(6.22±1.01)mm]and carotid IMT [(0.89±0.21)mm vs.(0.84±0.11)mm]in exercise group after treatment,P<0.05 all.Conclusion:Long-term exercise training can effectively reverse early arteriosclerosis lesion of carotid wall and effectively control blood pressure.

Objective The purpose of this study is to study the expression of miR -221 in cervical cancer tissues and its relationship with HPV infection .Methods HR-HPV infection was detected by HC2,and 30 cases of HR-HPV negative and 5 cases of HR-HPV positive cervical cancer tissues were collected .Mean-while,30 cases of normal cervical tissues in patients with benign disease were collected as control group .The ex-pression of miR -221was detected by RT -PCR,preliminarily investigating the relationship between miR -221 expression and the occurrence of cervical cancer and HPV infection .Through transfection of miR-221 and anti-miR-221 into HPV16-positive cervical carcinoma cell line Caski and HPV 16-negative cervical carcinoma cell line C33a,we observed the role of miR -221 on the migration and invasion of Caski cells and C 33a cells.Results Compared with normal cervical tissues , the expression of miR -221 in cervical cancer was significantly in-creased,the difference was statistically significant( P<0.01);and the expression of miR-221 was closely correl-ative to the patients with or without lymph node metastasis ,pathological grade and clinical stage ( P<0 .01 );the expression of miR-221 in HR-HPV positive cervical cancer tissues was higher than in HR -HPV negative cer-vical cancer tissues(P<0.01);transfection of miR -221 and anti-miR-221 could promote or downregulate C33 a and Caski cells migration and invasion ,and the changes between two groups had statistical significance ( P<0.05).Conclusion The increased expression of miR -221 in cervical cancer tissues is closely related to the oc-currence and development of cervical cancer and HPV infection .

AIM: To investigate the changes of alkaloid in Rhizorrm Coptidis and Fructus Evodiae before and after combination. METHODS: The content of berberine hydrochloride,palmatine hydrochloride,jatrorrhizine hydrochloride in Rhizoma Coptidis and Fructus Evodiae before and after combination were determined by TLCS.RESULTS : After combination of Rhizoma Coptidis and Fructus Evodiae,contents of berberine hydrochioride,palmatine hydrochloride,jatrorrhizine hydrochloride significantly reduced,among which the reduction of berberine hydrochloride was more obvious.CONCLUSION: In decoction,Fructus Evodiae appears to restrict the alkaloid from Rhizoma Coptidis in order to lower the side effect of Rhizoma Coptidis.

Objective To explore the mechanism of the skin lesion induced by oxidation hair dyes Methods The effects of subjects,such as oxidation hair dyes,hydrogen peroxide (H 2O 2) and p phenylenediamine (PPD) and their mixture which were main components of oxidation hair dyes,on the proliferation of rat's keratinocytes were detected by primary culture technique of rat's keratinocytes and MTT colorimetric assay Results All of the observed groups presented a certain inhibition on proliferation of the rat's keratinocytes,except the group exposed to PPD at the lowest dose for the shorter period The OD values decreased with the increase of exposure levels and periods Dose response relationship was observed between the exposure levels and inhibition rates of cell survival(the r values after 24 h exposue: r H 2O 2 =0 981, r PPD =0 949, r PPD-H 2O 2 =0 963, r hair dyes =0 887) Conclusion The skin lesion induced by oxidation hair dyes might associated with its cytotoxicity