Objective To investigate the correlation between cerebrovascular disease risk and homocysteine (Hcy).Methods One hundred patients with cerebrovascular disease who confirmed by CT included 50 cases of cerebral hemorrhage (cerebral hemorrhage group) and 50 cases of cerebral infarction (cerebral infarction group).Eighty healthy person were selected as control group.The plasma Hcy was determined in all the subjects.The risk factors induced cerebral vascular disease were analyzed by multiple linear regression analysis and the correlation between cerebrovascular disease risk and Hcy was investigated.Results The plasma Hcy in cerebral hemorrhage group and cerebral infarction group was higher than that in control group[(19.87 ± 5.23),(19.64 ± 4.98) μ mol/L vs.(14.54 + 3.58) μ mol/L,P ＜ 0.05].There was no significant difference in the plasma Hcy between cerebral hemorrhage group and cerebral infarction group (P ＞ 0.05).Multiple linear regression analysis showed that Hcy was independently associated with cerebral hemorrhage and cerebral infarction (P ＜ 0.05).Conclusion The plasma Hcy in patients with cerebrovascular disease is higher than that in the normal population,and Hcy is independently associated with cerebrovascular disease.The plasma Hcy has an important role for prediction and diagnosis of cerebrovascular disease.

Objective To investigate the expression and clinical significance of the estrogen receptor(ER) subsets ER?,ER? and Ki-67 in tissues of different thyroid diseases.Methods The pathological data of 93 patients with thyroid diseases were collected from Jun.2003 to Jun.2007,including thyroid carcinoma(56 cases),thyroid follicular adenoma(13 cases),nodular goiter(14 cases) and normal thyroid tissue(10 cases).S-P immunohistochemistry was performed to determine the expressions of ER?,ER? and Ki-67 in the thyroid tissues.Results ER? and ER? were expressed in the plasma and nuclei of thyroid epithelial cells,and ER? was also expressed in the stroma.The positive rates of ER? expression in cases with thyroid carcinoma,thyroid follicular adenoma,nodular goiter and normal thyroid tissue was 87.5%,69.2%,42.9% and 30.0%,respectively,with significant difference between carcinoma and goiter or normal thyroid tissue(P0.05).Conclusion ER? and ER? may play an aggravating effect on cell proliferation in the disease course of the thyroid carcinoma.The expressions of ER? and Ki-67 are related to invasion and metastasis of thyroid carcinoma.

The present study was designed to confirm the use of GM-CSF, made by the Beijing United Biological Engineering Company, in leucocytopenia after chemotherapy in 12 patients with malignant tumor. In patients with malignant tumor the interval leucocytopenia needed shortened 5 days compared with that in control subjects. At the same time GM-CSF could be resistant to infection significantly, especially in patients with complicated infection, and the antibiotic applying time decreased. In addition, clinical experence had shown that there was no obvious side-effects in general dosage. Thus, these findings indicate that GM-CSF is a kind of essential drug in radical chemotherapy in patients with tumor.

ObjectiveTo explore the effect of motor on functional recovery of the lower limb in hemiplegic patients after cerebral infarction. Methods62 patients recovering from cerebral infarction were divided into the control group (30 cases) and motor imagery group (32 cases). All patients received routine treatment and a motor relearning program. Patients in the motor imagery group were given mental practice in activities of the lower limb. They were assessed with Fugl-Meyer Assessments (FMA) and Modified Barthel Index (MBI) before and after treatment. ResultsThe scores of FMA and MBI were improved in the motor imagery group compared with those in the control group (P<0.05) after treatment. ConclusionMotor imagery can facilitate the recovery of lower limb function in patients with hemiplegia.

OBJECTIVE To evaluate the exposing risks among dental staff and improve their concerns for prevention. METHODS By checking and evaluating the various risk factors,including HIV,HBV,HCV,SARS infectious antigens,and mercury contamination in our hospital,we found and formulated effective ways for preventing occupational disease. RESULTS Most dental staff in our hospital effectively decreased the professional exposing risks by practising preventive strategies. CONCLUSIONS Accurate evaluation and practical preventive strategies are key factors to reduce the professional exposing risks for dental staff.

Objective To investigate the expression and clinical significance of the estrogen receptor beta isoforms and survivin in thyroid tumors. Methods The pathological data of 125 patients with thyroid tumors were, collected from june 2003 to june 2007 in our institution, including thyroid carcinoma (86 cases), thyroid follicular adenoma (39 cases) and normal thyroid tissue (10cases). SP immunohistochemistry was used to measure the expressions of ERβ and survivin in the thyroid tumors. Results ERβ not only was detected in the thyroid epithelial cell plasma and nuclear, but also in the stroma. The positive rate of ERβ in cases with thyroid carcinoma, adenoma and normal thyroid tissue were 83.72 %, 51.28 % and 20.00 % respectively. There was significant difference between carcinoma and the adenoma or normal thyroid tissue (P<0.05). The positive rate of survivin in cases with carcinoma, adenoma and normal thyroid tissue were 59.30 %, 17.95 % and 0 respectively. There was significant difference between carcinoma and the adenoma or normal thyroid tissue (P<0.05). The expressions of ERβ and survivin correlated with TNM staging and lymph node metastasis of thyroid carcinoma (P<0.05). Conclusion ERβ may play a role in accelerating proliferation in the occurrence of the thyroid carcinoma. The expressions of ERβ and survivin are related to invasion and metastasis of thyroid carcinoma.

Objective To explore the association between variation in genes related to lipid metabolism and the susceptibility of nonalcoholic fatty liver disease (NAFLD). Methods Obese children with fatty liver aged 6~18 years old were included. All of them got ultrasonic testing, routine examination and biochemical detection. In addition, the DNA of peripheral blood was extracted and the 36 target genes related to lipid metabolism were detected by next generation sequencing. Results In 368 obese children who met the inclusion criteria, 183 children (49.7%) were detected to have NAFL . 100 children with NAFLD and 100 children without NAFLD were randomly selected from obese children. The levels of body mass, waistline, alanine aminotransferase (ALT), triacylglycerol (TG), cholesterol, low density lipoprotein (LDL) and apolipoprotein B (ApoB) in NAFLD children were all higher than those in non-NAFLD children, and there were significant differences (P all<0.05). However, the levels of aspartate aminotransferase (AST), alkaline phosphatase (ALP), apolipoprotein A1 (ApoA1), fasting blood glucose, insulin, high density lipoprotein (HDL), free fatty acid (FFA) and insulin resistance index (HOMA-IR) were not significantly different between the two groups (P all>0.05). The levels of bilirubine in the two groups were within normal range. Logistic regression analysis showed that the genes of MTTP rs2306986 (OR=2.70, 95%CI: 1.38~5.27) and MTTP rs3792683 (OR=7.34, 95%CI: 2.04~26.50) that encode microsomal triglyceride transfer protein (MTTP or MTP), and the mutation of rs738409 (OR=2.11, 95%CI:1.31~4.48) in gene PNPLA3 that encode patatin-like phospholipase domain-containing protein 3 are the independent risk factors for the occurrence of the disease. Conclusion Genovariation of MTTP rs2306986, MTTP rs3792683, and PNPLA3 rs738409 may increase susceptibility to NAFLD in children.

OBJECTIVE To discuss the risk factors of professional exposure of dental health personnel and treatment measures. METHODS The management system of standardization,systematization,science and universal prevention were carried out and realized to maximally prevent the onset of dental hospital infection. RESULTS A set of feasible management system and measures about professional exposure for dental health personnel were drafted to make dental staff master the management standards of dental infection,realize the sterilization procedures of dental device and prevent dental infection maximally in dental hospital by implementing universal preventive measures during dental treatment. CONCLUSIONS The preventive measures and programs about professional exposure for dental health personnel must be structuralized.

Objective To assess the efficacy of oral erythromycin on the functional gastrointestinal dysmotility in neonates.Methods In this double-blind,randomized,placebo controlled trial,90 neonates consecutively admitted to the neonatal intensive care unit of Shenzhen Children's Hospital from Jan 2009 to Dec 2011 were enrolled and randomly divided into low-dosage erythromycin group ( LE group,n =30 ),highdosage erythromycin group ( HE group,n =30 ) and control group ( n =30).Patients received either erythromycin ( 3 mg/kg or 10 mg/kg) or equivalent normal saline with oral or nasal feeding every 8 hours one time for 14 d.The time to achieve half,three-quarters,and full enteral nutrition,the time of parenteral nutrition,and hospital length of stay were compared among each group.Results The time to achieve half,three-quarters,and full enteral nutrition in HE group [ ( 3.0 ± 0.5 ) d,( 6.2 ± 0.7 ) d,( 8.2 ± 1.0 ) d ] and in LE group [(6.2±0.5) d,(8.3 ±0.6) d,(10.6 ±1.1) d] were shorter than that in control group [(8.1 ±0.4) d,( 13.5 ± 1.0) d,( 15.7 ± 1.2) d] ( P ＜ 0.05 ).The duration of parenteral nutrition [ ( 14.2 ± 1.4) d vs (9.3 ± 1.2) d vs (7.8 ± 1.1 ) d ] and hospital length of stay [ ( 13.0 ± 1.4 ) d vs ( 8.1 ± 0.8 ) d vs ( 6.8 ±0.7) d] were significantly prolonged in control group compared with LE and HE groups,and there were significant differences among the three groups ( P ＜ 0.05).The incidence of liver injury and septicemia during the treatment of erythromycin were similar between HE group and LE group,but it was significantly lower than control group.No serious adverse effect such as prolongation of QT intervals,dysrhythmia associated with erythromycin treatment was found.Conclusion Oral erythromycin can be considered as a treatment for neonates with functional gastrointestinal dysmotility who fail to establish adequate enteral nutrition,and highdosage oral erythromycin is more effective than low-dosage.

Objective:To investigate the changes of left ventricular mass index(LVMI),plasma brain natriuretic peptide (BNP),interJeukin-6(IL-6)and interleukin-10(IL-10)in patients with congestive heart failure(CHF),and their values in risk stratification and prognostic evaluation of CHF thereof.Methods:Ninety-five patients with CHF in accordance with 2001ACC/AHA guidelines were enrolled and stratified as stage A(n=21),B(n=23),C(n=31)and D(n=20).The levels of plasma BNP,IL-6 and IL-10 were measured and compaired with those in 20 healthy subjects(control group).The patients were followed up every three months in two years.The end point was the rehospitalization and the cardiac death.Results:(1)The level of LVMI increased early in patients with CHF at stage B.(2)The plasma BNP levels were initially elevated in CHF patients at stage B,and the concentrations of BNP closely paralleled disease severity.(3)The levels of plasma IL-6 and IL-10 were increased,and the ratio of IL-10/IL-6 was decreased,with the progression of CHF at stage C and D.The plasma BNP level was positively correlated with the levels of IL-6 and IL-10.(4)The new CHF stratification was surperior to NYHA functional classification in regard to CHF prognosis prediction.(5)Multivariate COX regression analysis showed the levels of BNP and IL-6 were the independent risk factors in prognostic evaluation(P＜0.01,P=0.026).(6)Patients in high-risk groups stratified by the combination of BNP and stage classification had obviously higher end point events rate.Conclusion:(1)LVMI was a better marker for the evaluation of left ventrieular remodeling.(2)The intrinsic mechanism of CHF progression was inflammatory reaction deterioration.(3)BNP was a good marker in predicting onset and prognosis of CHF.The combination of BNP and the stage classification could improve the prognostic evaluation in patients with CHF.