Objective: To study the effects of soluble multi-peptide agent,marked “Gu Kang Tai Ling”,derived from bone tissue on rat bone fracture healing.Methods 80 right tibia defects of rats were sawed.The “Gu Kang Tai Ling”were intramuscularly injected to the rats.The quality of the defect healing was investigated continuously and respectively by the defect bone histomorphometry,biomechanics,X-ray film,and bone mineral density(BMD).Results: Under the treatment of the agent,at early stage trabecular bone volume (TBV)ratio increased 24.7%,and osteoblast surface(OBS)26.7%,at late stage mean trabecular healing surface rate(MTHSR)was increased 38.8%,tibia anti-fracture strength 0.4527～1.4350N，and BMD 7.65% compared with the control group.Conclusion: “Gu Kang Tai Ling”can accelerate rat fracture healing and improve the quality of healing.

Objective:To investigate the effect of curcumin on the implant osseointegration in the rats with osteoporosis, and to clarify its mechanism.Methods:A total of 45 rats were randomly divided into control group (screw implant), model group (osteoporosis model + screw implant) and curcumin group (osteoporosis model + screw implant + curcumin treatment), with 15 rats in each group. The micro-CT was used to examine the relative bone volume fraction (BV/TV), average bone trabecular thickness (Tb. Th), trabecular space (Tb.Sp), average bone trabecular number (Tb.N),osseointegration index(OI) of the bone tissue around the implants of the rats in various groups; methylene blue-basic fuchsin staining was used to examine the morphology of bone tissue;the bone contact rate and the bone mass in cancellous bone area were calculated. The torque tester was used to measure the implant dislocation torque.Results:Compared with control group, the BV/TV, Tb.Th, Tb.N, OI,the bone contact rates,the bone mass in cancellous bone area,and the dislocation torque of bone interface around the implants of the rats in model group and curcumin group were significantly decreased (P<0.01), while Tb.Sp in model group and curcumin group were significantly increased (P<0.01). Compared with model group, the BV/TV, Tb.Th, Tb.N, OI, the bone contact rate, the bone mass in cancellous bone area,and the dislocation torque of bone interface around the implants of the rats in curcumin group were increased (P<0.01),while Tb.Sp was significantly decreased (P<0.01). In control group, the bonding of implant and bone was close, and the bone plate and trabeculae at the bonding interface of implant and bone were thicker and denser. In model group, the bone plate at the bonding interface of implant and bone was thin and the trabecular bone was sparse. In curcumin group, the bone plate and trabeculae at bonding interface of the implant and bone of the rats were thicker and denser.Conclusion:Curcumin can promote the implant healing, improve the implant and bone bonding strength and increase the implant stability in the osteoporosis rats.

Objective To compare the X-ray cephalometric results of teeth and skeleton relationship between individual lingual orthodontic and labial straight wire orthodontics,and to provide reliable theoretical foundation for selecting orthodontic system in clinic. Methods The general conditions of the patients, classification of malocclusion,severity of denture crowding and orthodontic design were used as the selection criteria. Thirty familiar adult patients with malocclusion in clinic were divided into two groups randomly:individual lingual orthodontics group (A group)and labial straight wire orthodontics group (B group),15 cases in each group.X-ray cephalometrics were taken using Steiner and Tweed cephalometrics analysis,the results of analysis were compared between pre-treatment and post-treatment in two groups respectively, and the course of treatment in two groups was compared,too.The difference of treatment effect and course of treatment between two different orthodontic systems was evaluated.Results The relationship of first molar and canine of the patients in two groups was neutral relationship,overbite and overjet of anterior teeth was shallow,the teeth arrangement was orderly and no space was left after treatment.There was no statistically significant difference (P>0.05)between two groups in each measured values detected by Steiner Analysis after orthodontic treatment;there was no statistically significant difference (P>0.05)between two groups in each measured value detected by Tweed Analysis after orthodontic treatment;and there was no statistical significance (P>0.05 )in the D-values of Steiner Analysis and Tweed Analysis between two groups before and after treatment.The duration of treatment of the patients in two groups had no significant difference (P>0.05).Conclusion Both individual lingual orthodontics and labial straight wire orthodontics could obtain well teeth arrangement and occluding relationship and achieve satisfying clinical orthodontic outcome.

Objective To explore the surgical treatment of ankle fracture.Methods 326 cases with ankle joint fracture in our hospital treated by surgical treatment were followed up.There were 182 males and 43 females,aged from 7 ～ 75 years.The patients with medial malleolus fracture were cut through postero-medial process,and the lagged screw,absorbed screw,or tension band wire were used.Patients with bimalleolus and trimalleolus fracture were cut through medial and lateral two incision.When the fracture line of the fibula is high,the triangular needle,kirschner wire,plate and the screw,remember alloy were used,when the fracture line of the fibula was high,1 ～ 2 cortical or cancellous bone lagged screw were used.The patients with diastasis of inferior tibiofibular syndesmosis were cut through lateral process,1 lagged screw was used through 3 layer cortical bone.After the operation,the plaster or brace was used according to the stability of the fracture,then systemic functional exercises.Results All the patients were followed up from 4 ～ 120 months,according to the ankle-hind foot score of American Orthopedic Foot and Ankle Society Score(AOFAS),their clinical treatment results were assessed.In 225 cases,excellent and good rate was 86.7％.Connclusion Proper approach,anatomy reduction,articular surface recovery to smooth,stable internal fixation,necessary external fixation and systemic rehabilitation were the key aspects to the surgical treatment.Good result of most of the patients with fracture of the ankle joint can be obtained.

Objective To explore the value of tracheal intubation guided by fiberoptic bronchoscopy in children snoring disease operation.Methods A total of 50 patients underwent tracheal intubation guided by fiberoptic bronchoscope or laryngoscope from August 2015 to June 2016.There were 25 cases of fiberoptic bronchoscopy assigned into group A, 25 cases of laryngoscopy into group B.The intubation time, success rate and adverse reactions of the two groups were compared and analyzed.Results All the intubation in group A succeed at the first time.The intubation time was (20.1±4.3) s and the position was right.There was no need for replacement during the operation.Three cases in group B succeed at the second time because the tonsil was too big to expose the pharynx.The intubation got out of the pharynx in 3 cases in group B and were adjusted to the right position.The intubation time was (50.7±6.9) s and the success rate at the first time was 76% (19/25).Groups A behaved better than group B in aspects of the success rate of signal intubation and intubation time (Fisher''s Exact Test, P=0.022;t=-18.725, P=0.000).There were no adverse reactions in group A, such as pharyngorrhagia, odontoseisis or loss of tooth, laryngospasm and so on.There were 3 cases of hemorrhage from throat after intubation, 3 cases of loss of tooth in different degrees and 1 case of laryngospasm at second intubation time in group B.There was no significant difference in adverse reactions between the two groups (P>0.05).ConclusionFiberoptic bronchoscopy can be used for children snoring disease with good safety as well as rare adverse reactions.

Objective:To study the conditions of in vitro proliferation and differentiation of rat bone marrow stromal cells(MSC) for its application in transplantation.Methods:MSC were obtained by flushing the shaft with DMEM using a sterile syringe,dissociated by density gradient centrifuge,and cultured in the medium containing DMEM and 10% fatal bovine serum(FBS).Then we added neural growth factors into the medium and observed their influence on MSC proliferation,differentiation and survival by phase contrast microscopy.Immunocytochemistry was used to identify cell types.Results:MSC cultured in medium containing DMEM/ 10% FBS/ neural growth factors proliferated quickly.The neuron-like cells appeared at 7-9 d with the expression of neurone specific enolase(NSE) at(52?9.2)% and glial fibrillary acidic protein(GFAP) at(15?7)%,and could survive more than 7 days;In contrast,cells induced by ?-mercaptoethanol(BME) died gradually after 24 hours.Conclusion:MSC can not only proliferate and differentiate into neuron-like cells effectively in the medium containing neural growth factors but also survive longer.

Objective To study the protective effect of sodium ferulate (SF) on CPB-induced lung ischemia reperfusion injury (IRI) and discuss its possible mechanism. Methods 40 patients undergoing CPB were randomly divided into contral group(CCG, n=20 ) and SF group(SFG, n=20). In SFG, SF was injected (0.2 g in NS 150 ml) intravenously Bid for one week preoperatively. In CCG, SF was replaced by same volume of NS. The changes of TNF-?、IL-6、IL-8、SOD level in the CCG and SFG would be examined before operation, at 15min、30 min after aortic cross clamp(ACC) and 1 hour after operation. By the time of before ACC、15 min and 30 min after ACC was opened, blood samples were taken from the right and left atrium to examine the amount of neutrophils. Lung tissues were cut for pathological studies in 5 patients in each group randomly. Results At 15 min、30 min after ACC and 1h after operation TNF-?、IL-6、IL-8、SOD levels of 2 groups were significantly higher than that before the operation (P

0.05). The scaffolds produced no effect on the cell proliferation and had a low cytotoxicity, with chondrocytes tiled and lots of microspikes and matrixes secreted on the surface. Conclusion: The PCL-b-PLLA scaffold has a good compatibility with cells and is suitable for cartilage tissue engineering.

Objective To study the diagnosis and trea tm ent of multiple endocrine neoplasia type 2b. Methods On e case of MEN2b was reported.The clinical features,the diagnosis and treatment o f MEN2b were reviewed and presented. Results The diagno sis was established on B-ultrasonography and CT scanning,and the patient was cu red by operation. Conclusions MEN2b has been rare.Endoc rine check up,imaging procedures,family investigation and DNA analysis are very important to early diagnosis.Surgical removal of the tumors is effective.

Objective To study the biological functions of growth factors in the process of osteoblasts allografting and its acceleration in fracture healing in osteoporosis rats. Methods The fracture model of rat osteoporosis was established and then cell grafting was performed. Immunnohistochemistry and in situ hybridization were utilized to examine the expression of bFGF and bFGF mRNA during the process of fracture healing, the image was also analyzed. Results In the experimental group, bFGF positive cells were seen after 7 days after cell grafting, and peaked in chondrocytes about 14 day (384 65?8 60) after grafting. bFGF began declining after 21 days (286 24?2 30) and disappeared after 56 days. However in the control group, obvious high quanty was not seen and being only 125 33?4 50 even peaked at 21st day. Conclusions bFGF not only plays a very important role in bone formation but also enhances circulation formation in the fracture areas. It also accelerates the fracture healing in osteoporosis rats.