Objective:To analyze the differents in the upper airway morphology and hyoid position between skeletal class III malocclusion of high-angle and normal occlusion by cone beam CT(CBCT), and to study the influence of skeletal class III malocclusion of high-angle in the upper airway and hyoid position of the adults preliminarily.Methods:A total of 42 adults in Department of Orthodontics, Dalian Stomatology Hospital were chosen, including 21 adults with skeletal class III malocclusion of high-angle and 21 adults with normal occlusion. MIMICS 20.0 software was used to measure the line spacing, cross-sectional area and volume of each upper airway segment and line distance of hyoid of the patients on CBCT; SPSS 20.0 software was used for statistical analysis.Results:Compared with normal occlusion group, the maximum lateral distance (LAT1) of the nasopharynx, the maximum anterior-posterior distance (AP2) of the velopharyngeal, and the volume of the velopharyngeal (VOL2) of the patients in skeletal class III malocclusion of high-angle group were increased (P<0.05). Compared with normal occlusion group, the maximum lateral distance of the glossopharynx and laryngopharynx (LAT3 and LAT4), the cross-sectional area of the laryngopharynx (CSA4) and the volume of the laryngopharynx (VOL4) of the patients in skeletal class III malocclusion of high-angle group were decreased (P<0.05). While no statistically significant difference was found in the position of the hyoid bone that had a tendency to shift forward and upward of the patients in skeletal class III malocclusion of high-angle group compared with normal occlusion group (P>0.05)Conclusion:Cross-sectional area and volume of velopharyngeal have the tendency of increase, but cross-sectional area and volume of laryngopharynx have the tendency of decrease in the patients with skeletal class ? malocclusion of high-angle. The hyoid bone has a tendency to shift forward and upward in the patients with skeletal class ? malocclusion of high-angle.

OBJECTIVETo analyze mutations of SLC26A4 gene and explore their origins for a patient with enlarge vestibuar aqueduct syndrome.

METHODSClinical data and peripheral venous blood samples were collected from the patient and her parents. Genome DNA was extracted from the peripheral blood. All of the 21 exons of the SLC26A4 gene were amplified with PCR and subjected to directly sequencing.

RESULTSThe patient was found to have carried two mutant alleles of the SLC26A4 gene, namely c.1522A to G and c.1229C to T, which were inherited from her father and mother, respectively.

CONCLUSIONSLC26A4 c.1522A to G is likely to be a pathogenic mutation. Above results may facilitate genetic counseling and prenatal diagnosis for this family.

Adult ; Amino Acid Sequence ; Child ; Exons ; Female ; Hearing Loss, Sensorineural ; genetics ; Humans ; Male ; Membrane Transport Proteins ; genetics ; Molecular Sequence Data ; Pedigree ; Vestibular Aqueduct ; abnormalities

BACKGROUND:Human periodontal ligament stem cells are potential functional cells for periodontal tissue engineering.However,long-term in vitro culture may lead to reduced stemness and replicative senescence of periodontal ligament stem cells,which may impair the therapeutic effect of human periodontal ligament stem cells. OBJECTIVE:To investigate the effect of oxymatrine on the stemness maintenance and osteogenic differentiation of periodontal ligament stem cells in vitro,and to explore the potential mechanism. METHODS:Periodontal ligament stem cells were isolated from human periodontal ligament tissues by tissue explant enzyme digestion and cultured.The surface markers of mesenchymal cells were identified by flow cytometry.Periodontal ligament stem cells were incubated with 0,2.5,5,and 10 μg/mL oxymatrine.The effect of oxymatrine on the proliferation activity of periodontal ligament stem cells was detected by CCK8 assay.The appropriate drug concentration for subsequent experiments was screened.Western blot assay was used to detect the expression of stem cell non-specific proteins SOX2 and OCT4 in periodontal ligament stem cells.qRT-PCR and western blot assay were used to detect the expression levels of related osteogenic genes and proteins in periodontal ligament stem cells. RESULTS AND CONCLUSION:(1)The results of CCK8 assay showed that 2.5 μg/mL oxymatrine significantly enhanced the proliferative activity of periodontal stem cells,and the subsequent experiment selected 2.5 μg/mL oxymatrine to intervene.(2)Compared with the blank control group,the protein expression level of SOX2,a stem marker of periodontal ligament stem cells in the oxymatrine group did not change significantly(P>0.05),and the expression of OCT4 was significantly up-regulated(P<0.05).(3)Compared with the osteogenic induction group,the osteogenic genes ALP,RUNX2 mRNA expression and their osteogenic associated protein ALP protein expression of periodontal ligament stem cells were significantly down-regulated in the oxymatrine+osteogenic induction group(P<0.05).(4)The oxymatrine up-regulated the expression of stemness markers of periodontal ligament stem cells and inhibited the bone differentiation of periodontal ligament stem cells,and the results of high-throughput sequencing showed that it may be associated with WNT2,WNT16,COMP,and BMP6.