Objective To analyze the roentgenographic findings of the osteopetrosis. Methods Nine patients with clinically suspected osteopetrosis underwent the X-ray examinations of the chest,skull,pelvis,vertebral column,femur,tibiofibula,bones of upper limb and bilateral handbone. The roentgenographs of all patients were analyzed together with review of the literature. Results The basic image feature of osteopetrosis is bone density increased notably,cortible bone thickening,cavum ossis narrowing or occluded and mark X-ray signs include sandwich change in back bone,concentric rings change in pelvis,bone within a bone change,bright-dark alternation transverse striation in metaphysis of tubiform bone,baseball club appearance due to modelling deformity of long bones and periosteal new bone formation. Conclusion Osteopetrosis is a rare inherited disorder. Its mark roentgonographic findings are important evidence for performing the definite diagnosis and differential diagnosis.

Objective To discuss the application value of aer-barium sulphate double contrast radiograph in digestive tract diseases diagnosis. Methods The author analyzed 197 cases' images retrospectively which had positive performance in radiograph and had been clinical defined in our hospital from January to June 2008. Results In all these cases, there are 83 cases origin from mucous membrane which include inflammation, tumor and anabrosis; 24 cases origin below mucous membrane which include interstitialoma, lymphoma and scleroderma; 38 cases mainly changed in the shape, structure and position of organs which include hiatus hernia, Oblique inguinal hernia, diverticulum, malrotation of intestine and megacolon; 29 cases mainly changed in the power and function of organs which include functional disorder of esophagus, mesentery-compressed syndrome funtional disorder of small intestine and irritable colon; 17 cases' performance caused by the factor outside intestinal canal which include mesentery shift, stomach shift and small intestine shift, and other cases which include fistula between esophagus and mediastinum, rectovaginal fistula, foreign body in esophagus and small intestine ascariasis. Conclusion Aer-barim sulphate double contrast radiograph is the best method to diagnose the digestive tract diseases because it has forte as follow: convenient, easy prepared, less painful, large-scale examine limit and reliable diagnosis.

Objective To explore the diagnostic value of DDR in synovial sarcoma and compare with the traditional X-ray photography. Methods The DDR manifestation of synovial sarcoma patients which confirmed by operation in our hospital was contrasted with the traditional X-ray photography. Results The DDR performance of synovial sarcoma in soft tissue tumors,small bone destruction around the joints and soft tissue swelling,calcification of tumors are superior to traditional X-ray photography. Conclusion The DDR has better X-ray image quality than traditional X-ray,which can provide more diagnostic information and higher diagnostic value in this disease.

Objective To study the effect of human antisense VEGF gene on VEGF expression and growth of renal cell carcinoma Methods Reverse transcription-polymerase chain reaction (RT-PCR) for VEGF,VEGF was inserted into eukaryotic expression vector pcDNA3.1 to construct eukaryotic expression vector carrying human VEGF cDNA,then using restrict enzyme to confirm the result.The vector was transfected into renal cell carcinoma 780-0 and positive clone was selected by using G418.VEGF expression was detected by using immunocytochemical technique and the growth curve was detected by using MTT method. Results VEGF gene was gained by RT-PCR and antisense VEGF eukaryotic expression vector pcDNA 3.1 was constructed.The positive cell rate of VEGF expression in pcDNA 3.1-(antisense)VEGF group( 10.3%) is lower than that in 780-0-PC group(92.8%) or 780-0 group(96.6%), P

Objective To analyze the value of multi-slice spiral CT (SCT) scan in staging and subtyping of renal cell carcinoma (RCC). Methods The preoperative kidney SCT data and postoperative pathology results of 64 patients with RCC were retrospectively analyzed. The patients′ ages ranged from 33-78 years (average 54 years). There were 44 males and 20 females in the study group. According to the CUA Guidelines, the staging and subtyping of RCC were performed through the combined information of preoperative SCT attenuation in unenhanced, corticomedullary phase and enhancement pattern. The results were compared with the postoperative histopathological results. Results The SCT results showed 38 cases were clear cell RCC, 14 cases were papillary RCC and 12 cases were chromophobic cell RCC. Histopathological results showed that 40 cases were clear cell RCC, 16 cases were papillary RCC and 8 cases were chromophobic cell RCC. According to the standard of 40 HU of CT attenuation value, the sensitivity, specificity and accuracy were 75%, 79% and 78% for diagnosis of papillary RCC in the unenhanced phase. The sensitivity, specificity and accuracy by the standard of 90 HU of CT attenuation value was 90%, 88% and 89% for diagnosis of clear cell RCC in the corticomedullary phase. In chromophobic RCC, homogeneous enhancement was more common than in papillary RCC and clear cell RCC. There was no significant difference of staging and subtyping of RCC between SCT and pathological results (P>0.05). The accuracy of SCT in staging and subtyping of RCC was 88% in staging, and 89% in subtyping. Conclusions SCT is a useful preoperative tool to stage and subtype RCC

10mm in diameter) which were mostly non-functional ones(63.3%), and 12 microadenomas(≤10mm in diameter) which were mostly functional ones(91.7%). Only macroadenonas had abnormal imaging in DR, DSA, while microadenomas had no change in above radiological examinations. Microadenomas appeared in low density on CT or hypointensity in MRI as the direct signs, and CT, MRI contrast enhancement raised its display rate greatly. Macroadenomas showed various contrast in the post enhancement CT or MRI. Contrast enhancement of CT or MRI were capable of delineating size, location, extent of the tumor and the remaining intact pituitary tissue. Conclusion MRI Gd-DTPA contrast enhancement scaning is the first-selecting method to diagnose the pituitary adenomas especially the clinical suspected cases.

Objective To explore the exposure condition of DDR, screen -film radiography and the radiation dose to patients, and evaluate value of the DDR system. Methods Five thousand images of DDR and screen-film radiography were selected and then analyzed by three junior radiologists and two senior radiologists. Results 1.The quality of the images was classified into grades A ,B ,C: grade A in 92.8 % , grade B in 7.2 % , grade C in 0% ,waste in 0 % for DDR group and grade A in 40.8% , grade B in 41.7 %,grade C in 15.5 % ,waste in 2% for screen-film radiography group. 2. The required voltage of DDR system raises 3-24kV than screen-film radiography and radiation exposure was increased about 25 % . Conclusions The imaging quality of DDR was obviously higher than the screen -film radiography, but the disadvantage of DDR system was the higher exposure condition required, which increase X-ray radiation dose for patients.

Objective To assess the outcome of de-epithelialied ileocystoplasty combined with strengthened pelvic floor in patients with neurogenic bladder.Methods Twelve patients (9 male,3 female) aged from 18 -27 years (averaged 25 years) with neurogenic bladder received de-mucosalized seromuscular ileocystoplasty combined with strengthened pelvic floor,and were evaluated by urodynamic parmeters,upper urinary tract image appearance,and serum creatinine before and one year after operation.Results After operation,the max cystometric capacity (412 ± 32 ml),bladder compliance (26.2 ± 4.0ml/H2O),relative safety cystometric capacity (368 ±26 ml) and max flow rate (20 ±3 ml/s) were respectively significantly higher than those preoperation(247 ±27 ml,4.4 ± 1.2 ml/cm H2O,206 ±24 ml,11 ±2ml/s,P ＜ 0.05).Moreover,the post voided residual (26 ± 8 ml) and detmsor leakage point pressure (17.8 ±3.6 cm H2O) were significantly lower than those of preoperation (136 ± 25 ml,63.1 ± 4.9cm H2O,P ＜0.05).The vesicoureteral reflux disappeared in five (63％) cases,and was relieved in the remaining three cases.Of the five cases with renal insufficiency,three (60％) cases had normal serum creatinine level,none had increased serum creatinine levels.After operation,late healing occurred in two ( 17％ ) cases,intestinal obstruction in one (8％),vesicoabdominal fistula in one (8％),and no cases had mucous urine.Clean intermittent self-catheterization was performed in one case (8％) to empty the bladder due to a fever resulting from urinary tract infection,the remaining 12 (92％) cases could empty their bladders through abdominal pressure.Conclusions De-mucosalized seromuscular ileocystoplasty combined with strengthened pelvic floor results in a good outcome for the patients with neurogenic bladder.

Objective To investigate the relationships between genetic polymorphism of CYP2A6 alone or in combination with smoking and hereditary susceptibility to bladder cancer.Methods Based on case-control study,CYP2A6*4 was determined by the nested polymerase chianreaction(nPCR)in 186 patients with bladder cancer and 192 nontumorous controls.The relations between the genetypes of CYP2A6*4 alone or combinated with smoking and bladder cancer was estimated with the X2 test and logistic regression model.Results In the case subjects,the number of the wil/wil genetype was 168,the number of the wil/del genetype was 13,and the number of the del/del genetype was 5.In the control subjects,the number of the wil/wil genetype was 150,the number of the wil/del genetype was 32,and the number of the del/del genetype was 10.The frequency of CYP2A6 del allele was significantly lower in the case Subjects(9.68%)than the controls(21.88%,P＜0.05,OR:0.383).When eombinated with smoking,the risk of bladder cancer in smokers was significantly higher than nonsmoker(P＜0.05,OR=2.322).In smokers,the frequency of CYP2A6 del allele was significantly lower in cases(7.88%)than controls(28.00%,P＜0.05,OR=0.221).In smoking people,the one with CYP2A6 del genotype had a lower risk of bladder cancer than the one with CYP2A6 wild genotype(OR=0.221,95%CI:0.092,0.534).Conclusions Genetic polymorphisms of CYP2A6 are associated with the susceptibility to bladder cancer and have interaction with smoking in carcinogenesis of bladder cancer.Deficient CYP2A6 activity to genetic polymorphism mayreduee bladder cancer risk in smokers.

Objective To study the relationship between cyclooxygenase 2 (COX-2) polymor phisms and the susceptibility of bladder cancer.Methods Polymerase chain reaction restricted frag ments length polymorphism (PCR-RFLP) and the primer introduced restriction analysis (PIRA-PCR)assay were used to genotype the COX-2-765G/C, 1195G/A and 8473T/C polymorphisms in a case control study of 180 bladder cancer cases and 180 cancer free controls in a Chinese population.Re stilts The distribution of the genotype frequencies of 765G/C and 1195G/A were not statisticallydifferent between the cases and controls (P=0.582 for-765G/C and P=0.270 for-1195G/A).Poly morphisms of COX 2-8473T/C were associated with the susceptibility to bladder cancer.The individ uals with the 8473C allele had a decreased risk of bladder cancer (OR=0.56,95% CI=0.35 0.88).Conclusions Polymorphisms of COX-2-765G/C and-1195G/A are not associated with the suscepti bility to bladder cancer.However,COX-2-8473T/C can reduce the risk of bladder cancer.