OBJECTIVE To study fungal isolation and its drug resistance from abacterial body fluid.METHODS The abacterial body fluid for patients in our hospital was cultured by BacT Alert 120 and routine method.The strains which had been isolated were identified by development culture method and VITEK-YBC card or API-20C AUS reagent stripes.Furthermore,drug sensitive test was done by ATB FUNGUS2 drug sensitive reagent kit.RESULTS From the 898 samples with positive cultures of abacterial body fluid.8 species and 79 strains were isolated.Among the 79 fungal strains(8.8%),Candida albicans was 40 strains(50.6%),C.tropicalis was 12 strains(15.2%),C.glabrata was 11 strains(13.9%),C.parapsilosis was 9 strains(11.3%),C.guilliermondii was 4 strains(5.1%),C.krusei was 1 strain(1.3%),Cryptococcus were 1 strain(1.3%),and Mucor were 1 strain(1.3%).From them,13 strains(16.5%) were isolated in ICU,10 strains(12.7%) were in neurosurgical department,9 strains(11.4%) in oncological department,8 strains(10.1%) in urological surgery department,7 strains(8.9%) in thoracic surgery department,7 strains(8.9%) in pneumology department,6 strains(7.6%) in hematology department,5 strains(6.3%) in gerontology department,4 strains(5.1%) in orthopedic department,4 strains(5.1%) in endocrinology department,2 strains(2.5%) in nephrology department,and 4 strains(5.1%) in other units.The antifungal activity of amphotericin B,nystatin and flucytosine for fungus was quite perfect with sensitive ratio of 90%.But the drug resistance ratio to miconazole,ketoconazole and econazole was upward on 20-30%.CONCLUSIONS It′s important and necessary to monitor the circumstance of fungal infection and resistance of the pathogenic fungi due to its increased morbidity.

Objective Correctly identify Streptococcus pneumoniae resistant to optochin and other alpha hemolytic streptococcus susceptible to optochin.Methods Optochin susceptibility test, bile resolution test, latex agglutination test as well as some biochemical methods (VITEKCC4-GPI identifying cards,API-Strept identifying bar, VITEK TWO-GPC identifying cards) were applied.Results It was observed that 2 strains(0.3%)of Streptococcus pneumoniae out of 630 were resistant to optochin and 31strains of Streptococci, classfied as alpha hemolytic streptococci, susceptible to optochin, including 13 of S. mitis, 6 of S.oralis, 6 of S. twin, 3 of S. acidominimus, 2 of S.intermedius and 1 of S. constellatus. The inhibition zone of these alpha hemolytic streptococci susceptible sensitive to optochin was within the range of 14-17mm in diameter, in not 20 mm or more than 20 mm, compared with the inhibition zone of most strains of S.pneumoniae. Most of these alpha hemolytic streptococci (93.5%) susceptible to optochin showed highly resistance to benzoxazolecillin,to which most Streptococci (94%) showed sensitivity . Conclusion S.pneumoniae can be effectively identified by bile resolution test and latex agglutination test because of its high specificity with credible test results. API-Strept identifying bar and VITEK TWO-GPC identifying cards can be applied to identify S. pneumoniae resistant to optochin.

OBJECTIVE To know the distribution and drug resistance of the ventilator-associated pneumonia(VAP) bacteria in the intensive care unit(ICU),and to provide a reasonable basis for the clinical use of antibiotics. METHODS We used the Tiek Biagnostic systems to identifly microorganisms and antibiotic susceptibility.The result of the drug sensitivity test was analyzed with SPSS13.0. RESULTS A total of 538 strains were isolated from the sputum samples of the ventilator-associated pneumonia patients in the ICU over the last 4 years,in which Gram-negative bacteria were 361(the isolation rate 69.3%).Pseudomonas aeruginosa was the most common.Gram-positive bacteria were 143(the isolation rate 26.6%),in which Staphylococcus aureus was the most common.Twenty-two strain were fungi(the isolation rate 4.1%).Most of the pathogenic bacteria maintained high sensitivity rate to imipenem,vancomycin and quinupristin/daefopristin;but the drug resistance rate to other antibiotics was high and on the rise year by year. CONCLUSIONS The pathogens types of VAP was,complex,and multi-drug resistant.We should emphasize the reasonable application of the antibiotics and strengthen the monitoring of drug resistantce,and rational use of antibiotics to improve the cure rate.

Drug Evaluation, Preclinical ; methods ; Humans ; Intracellular Signaling Peptides and Proteins ; metabolism ; Protein Binding ; drug effects ; Small Molecule Libraries ; chemistry ; pharmacology ; Transcription Factors ; metabolism ; Tumor Suppressor Proteins ; metabolism