The experiments show that extract from Hippocampus trimaculatus Leach (EHT) can inhibit experimental common carotid artery and cerebral thrombosis in rats, and its effective ingredients are unsaturated fatty acids.

BACKGROUND:Creep is a visco-elastic biological material form of solid performance.It is necessary to understand the creep mechanical properties of middle cerebral artery in prevention and treatment of cerebral arteriosclerosis and cerebral hemorrhage.OBJECTIVE:To compare the creep rheological properties of middle cerebral artery between the normal and atherosclerotic animal model,to identify the effects of atherosclerosis on the creep properties of middle cerebral artery.METHODS:Male SD rats were randomly divided into 2 groups,in the model group,rats were injected vitamin D3+nicotine+ high-fat diet fed to prepare atherosclerosis models;in the control group,rats were fed with normal diet.Rats were killed at 4 weeks and the middle cerebral artery specimens were obtained.The creep experiments were performed with electron Shimadzu universal testing machine The stress rate was 0.01 Mpa/s,with 7 200 s experimental time.A total of 1 00 numbers were collected and analyzed using one linear regression analysis.The creep curve,as well as the variation of strain and time was recorded.RESULTS AND CONCLUSION:The middle cerebral artery creep changed faster at the initial 600 s in the normal and atherosclerotic rats,and then slowly decreased.The creep of normal rats were greater than that of the atherosclerotic artery specimens at the 7 200 s(50.38%,48.26%,P＜0.05).The results demonstrated that the middle cerebral artery have different mechanical properties of creep in normal and atherosclerotic rats.The creep curve is changed in an exponential relationship.and the atherosclerosis has certain effect on the creep characteristics of middle cerebral artery.

OBJECTIVE To investigate the resistance and the homology of the Acinetobacter baumannii(ABA) strains isolated from inpatients to offer basis for clinical treatment and preservation.METHODS VITEK2-compact system was used to identify 110 ABA strains,and detect their susceptibility to antibiotics.REP-PCR was carried out to detect their homology.RESULTS The 110 isolated strains were multidrug resistant.Rate of sensitivity to amikacin was 91.3%,Rates of resistance to ceftazadime,ciprofloxacin and imipenem were 100.00%,97.83% and 91.30%,respectively.REP-PCR was confirmed part of them was from the same clone.CONCLUSIONS Most of the isolated strains are multidrug-resistant.The same clone of MDR-ABA probably leads to transmission among patients.

Objective To describe the CT characterization of retroperitoneal neuroganglionic tumor in order to get a better understanding of the disease. Methods Neuroganglionic tumors proved by operation and pathology (24 cases), as well as bone biopsy (2 cases), were studied in 18 males and 8 females. The mean age of neuroblastoma(14 cases), ganglioneuroblastoma(10 cases), and ganglioneuroma (2 cases) was 1 year 3 months, 3 years 6 months, and 6 years, respectively. Two cases of ganglioneuroma were complicated with leukemia and malignant pheochromocytoma. All cases were examined by CT scanning and 14 of them had contrast enhancement scanning preoperatively. 20 cases were also examined by ultrasound. Results The tumor sizes were 2.0 cm?2.5 cm?2.5 cm～16 cm?10 cm?8 cm. The tumors were all located in the retroperitoneal space, 22 cases were unilateral, 3 were situated in mid line prelumboscrally, and 1 was bilaterally located. The tumor were irregular in 20 cases and round shape in 6 cases; the tumor margin showed distinct in 14 cases and indistinct in 12 cases. Twenty five tumors showed mixture density and isointensity comparing with the kidney, 1 case with homogeneouse low density; 12 of 14 cases were enhanced inhomogeneously and 2 did not enhance; 23 of 26 cases showed different shapes of calcification. Retroperitoneal lymph nodes were swelling in 19 cases. Liver metastases were found in 3 cases, vascular encasement in 18 cases, tumor thrombus in inferior vena cava in 1 case,and tumor extending into spinal canal in 3 cases. Conclusion CT scanning can prompt the diagnosis of retroperitoneal neuroganglionic tumor and bolus injection of contrast medium can further increase the understanding of the abnormality within and around the tumor.

AIM: Tranditional methods of screening drugs for benign prostatic hyperplasia(BPH)requires senile male animals such as dogs or rats.It consumes a long time to get the results. Over-expression of type Ⅱ5α-reductase in prastate induces BPH.A fast and efficient screening model of type Ⅱ5α-reductase inhibitors for BPH was set up in this paper.METHODS:Microsomes were extracted from male Sprague-Dawley rat livers by gradient centrifugation.Type Ⅱ5α-reductase enzyme-catalyzed reaction was assayed by UV-spectrophotometry using testosterone as a substrate and NADPH as hydrogen donor.The change of enzymatic activity was recorded with a NADPH wavelength of 340 nm by subtracted descending velocity of the control(without 5α-reductase).Effects at different conditions(temperatures,pH,enzyme and testosterone concentrations)on 5α-reductase were assayed.RESULTS:The suitable condition of type Ⅱ5α-reductase reaction Was defined as concentration of 109.05 mg protein/L enzyme(pH 6.00)with 2 μmol/L testosterone at 37℃.Michaelis'constant of type Ⅱ5α-reductase was 0.6μmol/L.Finasteride,a new drug for BPH,significantly inhibited activity of type Ⅱ 5α-reductase.IC50 of finasteride Was 64.1 nmol/L.As solvent of drugs,concentration of ethanol below 1.1% did not inhibite enzymatic activity(P＞0.05).Concentration of ethanol above 1.6%could obviouslv suppress enzymatic activity(P＜0.01).Daytime difference within five days had no significant difference(P＞0.05).CONCLUSION:A handy and fast screening method for type Ⅱ 5α-reductase inhibitors has been set up using UV-spectrophotometry.It may be used to screening drugs for BPH treatment.

Objective To investigate the effects of crocodilian plasma on 12 bacteria(including G+ bacteria and G-bacteria) lines in vitro.Methods The bacteria CFUs on nutrient broth agar plates were determined after bacteria and crocodilian plasma incubated together for 1h.Then effects of the samples on the survival rate of various concentrations of bacteria were calculated.To evaluate the antibacterial spectrum of crocodilian plasma,the zones of bacterial inhibition were measured through agar disk method.Results and Conclusion Crocodilian plasma had superior bactericidal effects on Escherichia coli M421C1,O111B4,E240-3 LT,Micrococcus catarrhalis,Shiga's bacillus,Pseudomonas aeruginosa and Diplococcus pneumoniae.The results indicated why wound of crocodilian by tearing each other was not susceptible,and crocodilian plasma could be developed a natural antibacterials.

0.05).Concentration of ethanol above 1.6 % could obviously suppress enzymatic activity(P0.05).CONCLUSION: A handy and fast screening method for type Ⅱ5?-reductase inhibitors has been set up using UV-spectrophotometry.It may be used to screening drugs for BPH treatment.