OBJECTIVE:To optimize the extracting technology of Nelumbo nucifera.METHODS:The extraction technology of Nelumbo nucifera was optimized by orthogonal experiment with the extraction rate of liensinine as index and with the concentration and amount of alcohol and the extraction time and extraction times as factors.RESULTS:The optimal conditions for extraction technology was as follows:adding four - fold amount of 80%alcohol and refluxing and extracting twice(1h each time).CONCLUSION:The extracting technology optimized under simulated conditions serves as a reference for industrial production.

0.05).The level of TGF-?1 in lung homogenate of model group was higher than blank group and Danshen group(P0.05).Conclusion TGF-?1 participates in invasion process of COPD and Danshen injection can interve COPD airway remodeling by down-regulating the level of TGF-?1.

The aim of the research is to introduce the kinds of medical Internet resources and worth of the Internet for the development of clinical, education and research of rehabilitation medicine. There are rich medical information in the internet and it is important for the rehabilitation medicine develol)ment. The strategies of exploring the rehabilitation medical internet resources are put forward.

The purpose of this study was to observe the neuromechanism and clinical effects of percu-taneous radiofrequency thermocoagulation cervical rarmi dorsales for the patients with headache of cervical origin. 16 patients were selected for the present study.Excellent result was obtained in thirteen cases.It is suggested that there was a close relationship between the injury of the cervical rami dorsales and headache of cervical origin,Radiofrequency thermocoagulation cervical rami dorsales plays a positive role in the treat-ment of headache of cervical origin.

After summing up the experience of recent years' clinic teaching, ICU department in Fangcun Branch of Second Clinical Medical College of Guangzhou University of TCM comes to a conclusion that, the clinical teaching of ICU should pay attention to and ensure effective implementation of beginning educationg. And following the PDCA method (Plan, Do, Check & Act) will make the work orderly, normative and controllable and meanwhile raise the students' observing and analysis ability as well as their operation and doctor-patient communication ability. In this way we can mostly enhance the effect of teaching.

OBJECTIVE:To provide reference for rational use of drugs for gastrointestinal ulcer. METHODS:The drug use for gastrointestinal ulcer in outpatient department of our hospital during 2013-2015 was analyzed retrospectively in respects of drug name,consumption sum,DDDs,DDC,single/multiple diseases prescription,therapeutic efficacy,rationality of presoription,etc. RESULTS:A total of 8 kinds of drugs use for gastrointestinal ulcer in outpatient department of our hospital during 2013-2015, among which the consumption sum and DDDs of Omeprazole capsule and Rabeprazole capsule increased rapidly,and their DDC were the higher others;those of other drugs kept stable. The proportion of acid-inhibitory drugs in single disease prescriptions was lower than in multiple diseases prescriptions,while that of antiacid was higher than in multiple diseases prescriptions,with statisti-cal significance (P<0.05). The total effective rate of patients with gastrointestinal ulcer gradually increased from 77.98% to 82.68% in outpatient department of our hospital during 2013-2015,there was statistical significance between 2015 and 2013(P<0.05);the proportion of irrational prescriptions decreased from 5.01% to 2.12% year by year,with statistical significance among those years (P<0.05). CONCLUSIONS:Omeprazole is the first choice for gastrointestinal ulcer in outpatient department of our hospital with good therapeutic efficacy. There still are some irrational drug use in prescriptions,and clinicians should pay attention to those situations.

In order to investigate the relationship between lichen planus pemphigoides (LPP) and bullous pemphigoid (BP) and to determine whether the LPP antigen is the classical BP antigen. Four LP patients who were found to have blisters or subepidermal bullae by clinical and histopathological findings were investigated using DIF, IIF, immunoblotting methods. The results showed that there were linear deposition of IgG along the BMZ in three cases, C3 granular deposition in four cases, and linear fibrin deposition in 3 cases. Two out of these patients showed circulating IgG anti-BMZ antibody with titers 1:80 and 1:320, and lmol/L NaCl split section showed that the antibody was bound to the epidermal side, as did control BP antisera. Western blotting of the epidermal extracts using the sera of these 4 patients showed that all 4 patients'sera reacted with the epidermal antigen, and the sera of 2 patients reacted with 200kD antigen which is different from classical bullous pemphigoid antigen. With a review of literature we suggest that LPP is not the overlapping of BP and LP.

AIM: To study the main components of volatile oil of Danggui Buxue Decoction (Radix Astragali, Radix Angelicae Sinensis) . METHODS:The main components of volatile oil were determined by GC-MS. The injector temperature was 250 ℃. The interface temperature was 230 ℃. The column flow was 1.3 mL?min -1. The column pressure was 100 kPa. The temperature rate was 3 ℃?min -1. RESULTS: The main components of volatile oil of Danggui Buxue Decoction were composed of trans-ligustilide, butylidene phthalide, n-butylphthalide, and cis-ligustilide. CONCLUSION: The main components of volatile oil of Danggui Buxue Decoction come mainly from volatile oil of Radix Angelicae sinensis.

AIM: To study the main components of the position of n-Butanol extract of Danggui Buxie Decoction(Radix Astragali,Radix Angelicae Sinensis). METHODS: The main components were determined by HPLC.HPLC with Nucleodur C18 Gravity column was used,the Acetonitrile-water(gradient elution) was used as a mobile phase and detecting wavelength at 210 nm. RESULTS: There were 14 common peaks in the peak position of n-Butanol extract of Danggui Buxue Decoction,Radix astragli had 14 peaks and Radix angelicae sinensis 3 peaks. CONCLUSION: The main components of the peak position among n-Butanol extract of Radix astragali and its extract and Danggui Buxie Decoction have correlation.

Object To establish the fingerprint of isoflavones and ferulic acid of Danggui Buxue decoction.Methods HPLC with Hypersil ODS column was adopted,the methanol-0.2 % acetic acid glacial(gradient elution) as mobile phase with a flow rate of 1mL/min and detecting wavelength at 254 nm.Results There were 10 main peaks in Danggui Buxie decoction,9 of them came from Radix astragali and 3 came from Radix angelicae sinensis.Conclusion This fingerprint can be used as a reference for the stability of the isoflavones and ferulic acid in Danggui Buxue decoction.