Objective:To explore the relationship between viral respiratory tract,mycoplasmal pneumonia,Chlamydia pneumonia infection and actue attack of asthma in children.Methods:7 viruses including respiratory syncytial virus(RSV),adenovirus(Adv),influenza virus A(InfA),influenza virusB(InfB),and parainfluenza virus 1.2.3(PInfl.2.3) from the nasopharyngeal aspirate of the patients were rapidly diagnosed by immunofluorescence method and MP.CP-IgM in serum were detected with ELISA.Results:18 cases could reach the definite etiological diagnosis among 45 infant asthmatic patients (40%).Of them RSV was discovered in 13 cases (accounting for 28.9%),ranking the first,second CP in 3 cases (6.7%),MP in 1 case (2.2%) and Plnf3 in 1 case (2.2%).13 cases (35.1%) were detected positively in 37 asthmatic patients of children.Of them MP was discovered in 9 cases (24.3%),ranking the first,second RSV in 4 cases (10.8%).Conclusion:The results indicate that infant asthma exacerbation is related closely to RSV and CP infections,but in children acute asthma exacerbation is related closely to MP infection.

Vitamin E( VE) is a major human plasma fat-soluble antioxidant. It has been proved that VE can protect the nervous system,skeletal muscle,retina and the cardiovascular system,etc. Nowadays,more and more researchers focus on the relationship between vitamin E and asthma. This article reviewes the research pro-gresses of the physiochemical properties of VE,and its relationship with asthma.

Bronchogenic cysts( BC) is a congenital malformation of the lung which is common in chil-dren and adolescents. Because the disease is rare and not characteristic in symptom,sign and imaging,it can be easily misdiagnosed. It is still difficult to improve the rate of correct and early diagnosis. This article reviewes the research progress of the occurrence,symptom and management of BC.

Objective To explore whether sodium and magnesium saline of fructose-1,6-diphosphate(Na +-Mg 2+-FDP)protects B cells in islet of Langerhans injured by streptozotocin(STZ).Methods Healthy adult male Wistar rats were randomly divided into five groups:STZ group(receiving once 2% STZ in citric acid solution at dose of 60 mg/kg),blank control group(receiving citric acid solution),FDP group(treated as STZ group,plus Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg?kg-1?d-1 for 15 d),protected group(receiving Na +-Mg 2+-FDP solution at 500 mg/kg first,then 2% STZ in citric acid solution at dose of 60 mg/kg once,Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg?kg-1?d-1 for 15 d),treated group(48 h after receiving 2% STZ in citric acid solution at dose of 60 mg/kg,starting Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg?kg-1?d-1 for 15 d).On the 15 th day after experiment starting,all rats were killed and the end of pancreatic gland was prepared for following experiments.Blood glucose by using glucose oxidase kit and serum insulin by radioimmunoassay was determined.The number of positive islets of which A,B and D cells expressed glucagons,insulin and somatostatin was counted by S-P immunohistochemistry.The morphology of islet of Langerhans stained by HE was observed under optical microscope.Statistical significance was determined by q test and ? 2 test,the related coefficient was determined by rectilinear correlation analysis(?=0.05).Results The blood glucose dropped and serum insulin rose in protected group,but the glucose in treated group and STZ group maintained at high level,and serum insulin maintained at low level.The blood glucose and insulin of the protected group showed significant differences with that of the treated group and STZ group respectively(P0.05).However,the expressions of insulin,gluca-gons and somatostatin were close to normal level in protected group,which were of statistical significance with that of STZ group and treated group(P

Examination is usually used to evaluate the students' understanding of the knowledge and skills.It is also the evaluation of teachers'clinical teaching effects to some extent.The objective of this paper is to discuss the new mode of clinical teaching according to the influence of clinical teaching of pediatric on examination.

Objective To evaluate the effect of clinical pathway management on pediatric capillary bronchitis. Methods Infants with capillary bronchitis admitted to our hospital were selected. Several indices were compared between the infants with and without clinical pathway management including hospital stay, costs of hospitalization, satisfaction in parents of children, cure rate, readmission rate one week after discharge, hospital infection and variation in the process of clinical pathway manage-ment. Results A total of 204 eligible infants were divided into research group (n=96) and control group (n=108). There were no signiifcant differences in sex, age, respiratory rate, heart rate and temperature, and detection rate of respiratory syncytial virus in nasopharyngeal secretion and sputum culture (P>0.05). Compared with the infants in control group, the total drug costs, the an-tibiotics costs and the average length of stay were signiifcantly decreased in infants with clinical pathway management (P<0.05). There were no signiifcant differences in such indices as satisfaction in parents of children, cure rate, readmission rate one week after discharge and hospital infection rate between two groups (P>0.05). In research group, 49 infants (51.04%) completed the clinical pathway management. Positive variance was found in 43 infants (44.79%) and negative variance in 4 infants (4.17%). Two infants (2.08%) dropped out. Conclusions For capillary bronchitis in infants, clinical pathway management has an effect on controlling and reducing the medical expenses, and meanwhile improving the medical quality and satisfaction of patients.

Objective To investigate the distribution of etiological agents in children with asthma exacerbation in Chongqing during 2013 .Methods Four hundred and forty seven cases of hospitalized children with asthma exacerbation in 2013 in Children′s Hospital of Chongqing Medical University were retrospectively analyzed in SPSS19 .0 software .Results Among the 447 children with asthma exacerbation ,the percentage of bacteria was 43 .8% ,streptococcus pneumonia(SP)(25 .5% ) was the most common bacteria .Among the 25 cases with bacteria culture of bronchoalveolar lavage ,the positive rate was 44 .0% ,and the difference to sputum culture was not significant (P>0 .05) .The positive rate of 7 common respiratory viruses was 27 .3% ,RSV accounted for 18 .8% ,and it was the most common virus .The virus infection rate was higher in the groups below one years old ,and compared each groups′virus detection rates ,the difference was significant (P< 0 .05) .The positive rate of mycoplasma pneumoniae was 23 .5% ,chlamydia pneumonia was not detected .Conclusion Asthma exacerbation in children is closely related to respiratory infec‐tion .Bacteria detection rate was high in all ages ,which suggests that bacterial infection is an important factor in asthma exacerbation that can′t be ignored .

Objective To evaluate needle localized operation biopsy (NLOB) and sterotactic core needle biopsy (SCNB) in the differential diagnosis of nonpalpable breast lesion (NPBL) found by mammography. MethodsIn 82 cases, a total of 90 NPBL were found, NLOB or SCNB were applied to make the diagnosis.ResultsBreast carcinoma (24.4%)was finally diagnosed in 20 cases, 61 cases were diagnosed with benign lesion. Thirty-five cases needed (42.7%) a surgery. Conclusion NLOB and SCNB provide new ways in establishing diagnosis of breast minimal lesions.

Objective To explore the clinical accuracy of urine cellular components of RBC,WBC,etc.detected by the Urit-1500 urine analyzer.Methods 1 085 urine samples in this hospital from June to October 2013 were selected and detected by the Urit-1500 urine analyzer and the microscope detection respectively.The results of urine cellular components detected by the urine analy-zer were analyzed.Results In 1 085 urine samples,the coincidence rate of two detection methods in detecting RBC and WBC was 93.8%(1 018/1 085)and 94.0%(1 020/1 085)respectively.In 295 RBC positive urine samples tested by the urine analyzer,the number of negative results tested by microscopy was 49,accounting for 16.6%.In 790 RBC negative urine samples tested by the u-rine analyzer,the number of negative results tested by microscopy was 18,accounting for 2.3%.In 197 WBC positive urine samples tested by urine analyzer,the number of negative results tested by microscopy was 28,accounting for 14.2%.In 888 WBC negative urine samples by urine analyzer,the number of negative results tested by microscopy was 37,accounting for 4.2%.Conclusion De-tecting urine RBC and WBC by using the Urit-1500 urine analyzer should combined with the microscopy and other test indexes and even clinical data to conduct the comprehensive analysis for reducing the misdiagnosis and missed diagnosis as far as possible.

Objective To study the value of needle aspiration biopsy guided by computerized mammography locator in the diagnosis of breast disease. Methods Needle aspiration biopsy was performed in the suspected areas guided by computerized mammography locater. Pathological diagnosis of this tissue taken by needle aspiration biopsy was compared with those taken through operation. Results There were 79 cases in this series. All the 79 specimens taking from needle aspiration confirmed to the requirement of biopy. Of them, 68 cases underwent operation, and the accurat pathologic diagnostic rate of needle aspiration specimens in the 68 cases was 97.2%.Conclusions Computerized mammography locator guiding needle aspiration biopsy is a simple and less invasive procedure.It is very useful for diagnosis of early breast cancer.