Celiac disease is an immunity mediated systematic disease triggered by dietary gluten in susceptible individuals,which is characterized by villous atrophy and malabsorption in small intestine (jejunum in particular),the symptoms can be relieved by exclusion of gluten (wheat,rye,barely)from diet.Worldwide epidemiological data confirmed that the disease is much more common than used to believe and the incidence is increasing.This article reviewed the progress in diagnosis of celiac disease.

Objective To evaluate the value of sterotactic mammorgraphy core needle biopsy in the diagnosis of nonpalable breast lesion. Methods 32 cases in this study were diagnosed by SMCNB,of whem,21 cases were operated on.Pathologic findigs of the tissues through SMCNB were compared with those of tissues by operation. Results Accuracy of diagnosis from SMCNB was 95%,sensitivity 80%,and specificity 100%.False negative result was found in 1 case.No false positive result occurred. Conclusions SMCNB is a simple and less invasive procedure with accurate location.It is valuable in the diagnosis of nonpalpable breast lesions.

Objective To explore the effects and possible mechanism of baicalin on renal interstitial fibrosis in unilateral urethral obstruction (UUO) rat.Methods Forty male SD rats were randomly divided into the sham operation group,model group,baicalin group,benazepril group and baicalin+ benazepril group,8 rats in each group.The baicalin group received the gavage of baicalin 40 mg · kg-1 · d 1,the benazepril group received the gavage of benazepril suspension 10 mg · kg-1 · d-1,the rats baicalin+ benazepril group received the gavage of baicalin 40 mg · kg-1 · d 1 and benazepril suspension 10 mg · kg-1 · d-1,the model group and sham operation group were given the same volume of normal saline.Rats were sacrificed in each group on 14,21,28 d after model construction and the kidney sample was obtained.Immunohistochemistry was used to detect the expression of TGF-β1 and α-SMA.Results Compared with the sham operation group,the TGF-β1 and α-SMA expression levels of renal tissue in the model group were significantly increased(P＜0.05),compared with the model group,the TGF-β1 and α-SMA expression levels on 14,21,28 d of model construction in the baicalin group,baicalin+ benazepril group and benazepril group were decreased to different extents (P＜0.05).But the TGF-β1 and α-SMA levels at each time point had no statistical difference among the baicalin group,benazepril group and baicalin+benazepril group(P＞0.05).Conclusion Baicalin may delay the progress of renal interstitial fibrosis in UUO rat by down-regulating the TGF-β1 expression and reducing α-SMA expression.

Objective To evaluate the clinical application of mammorgraphy with core needle biopsy in the diagnosis of breast lesions.Methods 36 cases of clinical nonpalpable breast lesions were detected with stereotactic needle core biopsy(SCNB) and 27 cases of ≥2 cm breast masses with direct core biopsy. Results The study showed that breast cancer was 20(31.7%), benign breast lesion 43(68.3%). The diagnostic accuracy, aspiration misplay and false negatigve were 93.6%, 3.2% and 3.2%, respectively. No false positive was found. Conclusion Mammorgaphy with core needle biopsy is a simple, less invasive and accurate localization procedure, very valuable in the diagnosis of nonpalpable lesions that only be visible on mammography.

Objective To evaluate the clinical value of elasticity imaging and area ratio by ultrasonography in differential diagnosis of benign and malignant thyroid nodules.Methods Gray-scale ultrasound and elasticity imaging was used to examine 88 patients with thyroid nodules.The elasticity classification and area ratio were retrospectively reviewed and compared with pathology.The elasticity grades 1 - 3 predicted benign,grades 4 - 5 predicted malignant.Results Eighty-eight patients with 116 thyroid nodules were detected,93 nodules were benign and 23 nodules were malignant.( 1 )An elasticity grades of 1 - 3 was observed in 82 (95.3％) of 86 benign nodules,while elasticity grades of 4 - 5 in 19 (63.3％) of 30 malignant nodules.The diagnosis sensitivity,specificity and accurate rate of the elasticity grades was 82.6％,88.2％,87.1％.(2) The mean of elasticity imaging and area ratio of 93 benign nodules (1.31 ± 0.13 ) was statistically lower than that in 23 malignant nodules ( 1.73 ± 0.13 ) (t =13.536,P =0.001 ).( 3 )According to ROC analysis,the cut-off point of elasticity imaging and area ratio was determined as 1.52.With elasticity imaging and area ratio ＜ 1.52,89 nodules [98.9％(89/90)] were confirmed as benign and 22 nodules [84.6％ (22/26)] were confirmed as malignant by pathology.The diagnosis sensitivity,specificity and accurate rate of elasticity imaging and area ratio was 95.7％,95.7％,95.7％.(4)The area under the ROC curve of elasticity imaging and area ratio ≥ 1.52 was 0.996,significantly higher than that of elasticity grades ≥ 4 (0.891).The diagnostic accurate rate of elasticity imaging and area ratio was significantly higher than that of elasticity grades(95.7％ vs.87.1％,x2 =5.472,P=0.019).Conclusions The elasticity imaging and area ratio by ultrasonography can be used in the differential diagnosis of thyroid lesions.It is a new diagnostic indicator for diagnosis of thyroid lesions.

Objective:To study the expression levels of nuclear factor kappa B, Bcl-2 associated K and TNF-αproteins to discuss the effects of NF-κB and Bak proteins in the pathogenesis of UC.Methods:Eighty clean grade of adult Sprague-Dawley( SD) rats were used,male and female in half and then rando mly selected sixty as the model group,another twenty as the control group.SD rats model were manufactured by a compound method:Trinitrobenzene sulfonic acid ( TNBS )+ethanol.We observed and assessed colonic mucosa by the general morphology and histological changes.To applicated immunohistochemistry and RT-PCR methods to detected the protein and mRNA expression levels of NF-κB,Bak and TNF-αin the model groups and the control group and to analysed their relationships.Results:The successful rate of making model was 97%.The number of inflammatory cells in the model groups more than the control(P<0.01).Group immunohistochemical and RT-PCR,NF-κB and TNF-αproteins and mRNA in UC colon epithelium cells and inflammatory cells were higher than the control(P<0.01).Bak protein in inflammatory cells were lower than the control(P<0.01),but there was no statistical significance in epithelial cells(P>0.05).The expression levels of NF-κB,TNF-αincreased as the histological grade increased(P<0.05),however,the expression level of Bak decreased(P<0.05).NF-κB in colonic mucosa of rats with UC had a significantly positive correlation with that of TNF-α(r=0.892,P<0.01),and negatively correlated with that of Bak(r=-0.793,P<0.01).Conclusion:The levels of NF-κB and Bak may be related to the occurrence and development of UC.

Objective To study the value of needle aspiration biopsy guided by computerized mammography locator in the diagnosis of breast disease. Methods Needle aspiration biopsy was performed in the suspected areas guided by computerized mammography locater. Pathological diagnosis of this tissue taken by needle aspiration biopsy was compared with those taken through operation. Results There were 79 cases in this series. All the 79 specimens taking from needle aspiration confirmed to the requirement of biopy. Of them, 68 cases underwent operation, and the accurat pathologic diagnostic rate of needle aspiration specimens in the 68 cases was 97.2%.Conclusions Computerized mammography locator guiding needle aspiration biopsy is a simple and less invasive procedure.It is very useful for diagnosis of early breast cancer.

Objective To investigate the relationship between gallbladder wall-typing and the main portal vein, right portal vein hemodynamics in patients with liver cirrhosis. Methods The main portal vein, right portal vein hemodynamics and the thickness of gallbladder wall were examined in 69 patients with cirrhosis and 30 normal subjects (control group) with two-dimensional ultrasound, color Doppler ultrasound and high-frequency ultrasound. According to the gallbladder wall-typing, 69 patients with liver cirrhosis were divided into not thickened, simple thickening and bilateral subgroups. The paremeters of hemodynamics and the thickness of gallbladder wall were compared among groups. Results There was statistical difference in thickness of gallbladder wall among all 3 subgroups. Statistical difference of the diameter of main portal vein (D_(pv)) was found among the subgroups (P<0.01), except between not thickened and simple thickening subgroup. Statistical difference of the velocity of the main portal vein (V_(pv)) was found between simple thickening subgroup and control group, as well as between bilateral subgroup and the control group (P<0.01). Statistical differences of quantity of blood flow in the main portal vein (Q_(pv)) were detected between bilateral subgroup and other 2 subgroups and control group (P<0.05). No statistical difference of diameter of right portal vein (D_(rpv)) and quantity of blood flow in the right portal vein (Q_(rpv)) was found among all groups and subgroups. The velocity of the right portal vein (V_(rpv)) of bilateral subgroup was statistically different with that of the control group and the simple thickening subgroup (P<0.05). No statistical difference of Q_(rpv)/Q_(pv) existed between the patients with liver cirrhosis and the normal subjects (P>0.05). There was positive correlation between the gallbladder wall thickness and D_(pv) (r=0.886, P<0.01). Conclusion The gallbladder wall-typing has no correlation with the right portal vein hemodynamics, but is related with the main portal vein hemodynamics. The bilateral and the thickness of gallbladder wall can indirectly reflect the hemodynamic changes of main portal vein. Obstruction to flow of cystic vein is not the main hemodynamic cause for the gallbladder wall changes in patients with liver cirrhosis and portal hypertension cause, indicating that there may be some intrinsic mechanisms of this phenomenon.

Objective To evaluate needle localized operation biopsy (NLOB) and sterotactic core needle biopsy (SCNB) in the differential diagnosis of nonpalpable breast lesion (NPBL) found by mammography. MethodsIn 82 cases, a total of 90 NPBL were found, NLOB or SCNB were applied to make the diagnosis.ResultsBreast carcinoma (24.4%)was finally diagnosed in 20 cases, 61 cases were diagnosed with benign lesion. Thirty-five cases needed (42.7%) a surgery. Conclusion NLOB and SCNB provide new ways in establishing diagnosis of breast minimal lesions.

The non-human primates have been profoundly being used to study the human reproductive models .It is the prerequisites to set up the operating rooms including hardware and software on reproductive experiments and transgenic manipulation.Thus, Combined with the practice of our center , both the construction and the associated problems about the IVF-ET operating rooms were discussed in this paper .It will provide some help for the related researches .