1.CD200 attenuates methamphetamine-induced microglial activation and dopamine depletion.
Xia, YUE ; Dongfang, QIAO ; Aifeng, WANG ; Xiaohui, TAN ; Yanhong, LI ; Chao, LIU ; Huijun, WANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2012;32(3):415-21
This study examined the neuroprotective effect of cluster of differentiation molecule 200 (CD200) against methamphetamine (METH)-induced neurotoxicity. In the in vitro experiment, neuron-microglia cultures were treated with METH (20 μmol/L), METH (20 μmol/L)+CD200-Fc (10 μg/mL) or CD200-Fc (10 μg/mL). Those untreated served as control. Microglia activation expressed as the ratio of MHC-II/CD11b was assessed by flow cytometry. The cytokines (IL-1β, TNF-α) secreted by activated microglia were detected by enzyme-linked immunosorbent assay (ELISA). In the in vivo experiment, 40 SD rats were divided into control, METH, METH+CD200-Fc and CD200-Fc groups at random. Rats were intraperitoneally injected with METH (15 mg/kg 8 times at 12 h interval) in METH group, with METH (administered as the same dose and time as the METH group) and CD200-Fc (1 mg/kg at day 0, 2, 4 after METH injection) in METH+CD200-Fc group, with CD200-Fc (1 mg/kg injected as the same time as the METH+CD200-Fc group) or with physiological saline solution in the control group. The level of striatal dopamine (DA) in rats was measured by high-performance liquid chromatography (HPLC). The microglial cells were immunohistochemically detected for the expression of Iba-1, a marker for microglial activation. The results showed that METH could increase the microglia activation in the neuron-microglia cultures and elevate the secretion of IL-1β and TNF-α, which could be attenuated by CD200-Fc. Moreover, CD200-Fc could partially reverse the striatal DA depletion induced by METH and reduce the number of activated microglia, i.e. Iba-1-positive cells. It was concluded that CD200 may have neuroprotective effects against METH-induced neurotoxicity by inhibiting microglial activation and reversing DA depletion in striatum.
2.Advancement on the diagnosis of anaphylactic shock in forensic medicine
Jinguo CHEN ; Dongfang QIAO ; Xia YUE
Chinese Journal of Forensic Medicine 2017;32(4):359-362,367
It is still an important and hard work to diagnose anaphylactic shock in forensic practice. However, no breakthrough progresses in the diagnosis of anaphylactic shock and relevant research have been made so far due to the problems we used to meet in actual postmortem examination ,which are short of specific pathological changes in autopsy, the condition progress of patients who occur anaphylactic shock and history of allergy. Furthermore, patients suffer from diseases such as coronary heart disease, pneumonia, asthma, skin irritation, etc, and blood serum allergy biomarkers degrade after hemolysis on account of a long time from death to autopsy ,which are also the difficulties we have to cope with. The aim of this review is to focus on present situation and diagnostic index of anaphylactic shock including the pathological changes and some experimental methods such as special stain, immunohistochemical and serological test to provide reference for diagnosis and study of anaphylactic shock.
3.Regulation of Naoxin'an Capsule on Glial Cell Activation and Inflammatory Response in Rats with Chronic Cerebral Hypoperfusion-induced Vascular Cognitive Impairment
Guang-hui HAN ; Dong-yue LI ; Hong-ni YU ; Wei-zhe ZHEN ; Tao MA
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(19):46-55
Objective:To investigate the protective effect of Naoxin'an capsule (NC) against glial cell activation and inflammatory damage in brain of rats with chronic cerebral hypoperfusion-induced vascular cognitive impairment (VCI). Method:One hundred and fifty rats were randomly divided into a sham operation group (
4. Effect of Chaibei Zhixian Decoction and Its Absorption Component Peimine on Content of Carbamazepine in Rat Brain and Investigation of Its Mechanism
Chong-chong LIU ; Jiang-yan SUN ; Xiao-ke DONG ; Kai-yue WANG ; Zhong-hao LI ; Jing WU ; Li-li LI ; Jin-min LIU
Chinese Journal of Experimental Traditional Medical Formulae 2019;25(19):32-38
Objective:To observe the effect of Chaibei Zhixian decoction and peimine on Carbamazepine (CBZ) concentration, P-glycoprotein (P-gp) and multi drug resistance 1(MDR1) expression in the brain tissues of rats with refractory epilepsy, and to understand the contribution of Peimine in the compound prescription to treat the refractory epilepsy. Method:Epilepsy rat models were established by injecting kainic acid (KA) in the lateral ventricle. The successfully modeled rats were randomly divided into model group, CBZ group(0.12 g·kg-1),Chaibei Zhixian decoction+CBZ group(8.39 g·kg-1+0.12 g·kg-1), peimine+CBZ group(0.01 g·kg-1+0.12 g·kg-1) and sham operation group. After 60 days of intervention, the expression levels of P-glycoprotein (P-gp) and MDR1b mRNA in the brain cortex were detected by Western blot and quantitative real\|time fluorescence polymerase chain reaction(Real-time PCR),the contents of CBZ and 10,11-epoxidation of carbamazepine (CBZE) were measured by liquid chromatography-mass spectrometry (LC-MS). Result:Compared with sham group, the expression of P-gp/MDR1 in the cortex of model group was significantly increased (P<0.05, P<0.01).Compared with model group, the P-gp/MDR1 level in CBZ group was increased.The expression of P-gp/MDR1 in the cortex of Chaibei Zhixian decoction+CBZ group and peimine+CBZ group was reduced. Compared with CBZ group, The expression of P-gp/MDR1 was significantly decreased in the cortex of Chaibei Zhixian decoction+CBZ group and peimine+CBZ group (P<0.05,P<0.01), and the content of CBZ and CBZE in the brain of peimine+CBZ group was significantly increased (P<0.05,P<0.01), and the content of CBZE in the brain of the Chaibei Zhixian decoction+CBZ group was significantly increased (P<0.05).Compared with the Chaibei Zhixian decoction+CBZ group,the content of CBZ and CBZE in the brain of the peimine+CBZ group was increased in rats, but the difference was not statistically significant. Conclusion:Chaibei Zhixian decoction and peimine may increase the content of CBZ and CBZE in the brain tissues in rats with intractable epilepsy by reducing the expression of MDR1/P-gp in the cortex.
5.Effect of Huoxue Jiedu Runzao Prescription on Expression of Apoptosis Inhibitor Survivin in Submandibular Gland Cells of Sjögren's Syndrome Mice
Fang-kai LI ; Yue-lan ZHU ; Xiu-juan HOU ; Yu-ting ZHANG ; Xin-yi GU ; Hai-bo WANG ; Qian SHEN
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(24):84-91
Objective:To investigate the effect of Huoxue Jiedu Runzao prescription on the morphology, apoptosis, and function of submandibular gland in the mouse model of Sjögren's syndrome (SS) and its functioning mechanism, we analyzed the expression of the apoptosis inhibitor Survivin in the submandibular gland cells of SS mice. Method:Female BALB/c57 mice were selected as the normal group. The naive non-obese diabetic (NOD/Ltj) female mice were selected as the SS model, which were randomly assigned into the model group, Paeoniae Radix Alba total glucosides capsule (0.234 g·kg-1) group, and low-, medium-, and high-dose (15.6, 31.2, 62.4 g·kg-1, respectively) Huoxue Jiedu Runzao prescription groups. Each group had 15 mice. The morphological and functional changes of submandibular gland and the Survivin expression were observed and measured after 8 weeks of drug intervention. Survivin expression was determined by immunohistochemistry (IHC), Western blot, and reverse transcription-polymerase chain reaction (RT-PCR). Result:Compared with normal group, salivary flow and submandibular gland index in model group were significantly decreased (
6.Relationship between PMP, FN, vWF and Bleeding Degree in Patients with Acute Leukemia.
Chang-Sheng WANG ; Lian ZHAO ; Li-Yun HUANG ; Xiao-He YUE
Journal of Experimental Hematology 2018;26(3):722-726
OBJECTIVETo detect the serum levels of platelet microparticle (PMP), fibronectin (FN), and von Willebrand Factor (vWF) in acute leukemia (AL) patients with thrombocytopenic and to analyze the relationship of the serum levels of PMP, FN and vWF with bleeding degree.
METHODSOne hundred and one newly diagnosed AL patients from May 2014 to May 2017 were enrolled the AL group. According to the WHO standard of bleeding stratification, 101 AL patients were divided into 5 sub groups: 0, 1, 2, 3 and 4 score groups; 52 normal persons subjected to physical examination were enrolled in control group. The PMP level was detected by flow cytometry; the FN and vWF levels were detected by ELISA. The levels of PMP, FN and vWF were compared between the AL group and the control group. The serum levels of PMP, FN and vWF were compared according to bleeding degree group. The relationship of bleeding degree with the serum levels of PMP, FN and vWF was analyzed.
RESULTSThe patients with newly diagnosed acute leukemia aged 18 to 60, and accounted for 61.39%. The degree of bleeding was mainly 1 score, which accounted for 38.61%. The serum levels of PMP, vWF and FN AL groups were significantly higher than those in control group (6.06%±4.38% vs 0.89%±0.50%, 205.82±24.89 vs 58.04±13.35 µg/L, 398.29±46.93 vs 311.37±26.02 µg/L)(P<0.001). The serum levels of PMP, FN and vWF were different among 5 subgroup (P<0.01); the level of PMP and FN were the highest in 0 score group and the lowest in 4 score group; the vWF level was the highest in 4 score groups and the lowest in 0 score group. The bleeding degree in the patients with acute leukemia negatively correlated with PMP level, and positively with NF and vWF levels (r=-0.753, r=0.648, r=0.805).
CONCLUSIONAccording to the relationship of the bleeding degree with serum levels of PMP, FN, vWF in patients, the detection of PMP, vWF and FN levels can help to evaluale the bleeding degree in the patients.
Acute Disease ; Adolescent ; Adult ; Cell-Derived Microparticles ; Hemorrhage ; Humans ; Leukemia ; Middle Aged ; Young Adult ; von Willebrand Factor
7.CD200 attenuates methamphetamine-induced microglial activation and dopamine depletion.
Xia YUE ; Dongfang QIAO ; Aifeng WANG ; Xiaohui TAN ; Yanhong LI ; Chao LIU ; Huijun WANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2012;32(3):415-421
This study examined the neuroprotective effect of cluster of differentiation molecule 200 (CD200) against methamphetamine (METH)-induced neurotoxicity. In the in vitro experiment, neuron-microglia cultures were treated with METH (20 μmol/L), METH (20 μmol/L)+CD200-Fc (10 μg/mL) or CD200-Fc (10 μg/mL). Those untreated served as control. Microglia activation expressed as the ratio of MHC-II/CD11b was assessed by flow cytometry. The cytokines (IL-1β, TNF-α) secreted by activated microglia were detected by enzyme-linked immunosorbent assay (ELISA). In the in vivo experiment, 40 SD rats were divided into control, METH, METH+CD200-Fc and CD200-Fc groups at random. Rats were intraperitoneally injected with METH (15 mg/kg 8 times at 12 h interval) in METH group, with METH (administered as the same dose and time as the METH group) and CD200-Fc (1 mg/kg at day 0, 2, 4 after METH injection) in METH+CD200-Fc group, with CD200-Fc (1 mg/kg injected as the same time as the METH+CD200-Fc group) or with physiological saline solution in the control group. The level of striatal dopamine (DA) in rats was measured by high-performance liquid chromatography (HPLC). The microglial cells were immunohistochemically detected for the expression of Iba-1, a marker for microglial activation. The results showed that METH could increase the microglia activation in the neuron-microglia cultures and elevate the secretion of IL-1β and TNF-α, which could be attenuated by CD200-Fc. Moreover, CD200-Fc could partially reverse the striatal DA depletion induced by METH and reduce the number of activated microglia, i.e. Iba-1-positive cells. It was concluded that CD200 may have neuroprotective effects against METH-induced neurotoxicity by inhibiting microglial activation and reversing DA depletion in striatum.
Animals
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Animals, Newborn
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Antigens, CD
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administration & dosage
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Cells, Cultured
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Coculture Techniques
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Corpus Striatum
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cytology
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drug effects
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immunology
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Cytokines
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immunology
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Dopamine
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immunology
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Drug Interactions
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Male
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Methamphetamine
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toxicity
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Microglia
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drug effects
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immunology
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Neurons
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metabolism
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Rats
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Rats, Sprague-Dawley
8.Research progress on fatigue in recipients of renal transplantation
Yue LI ; Yingzi MING ; Quan ZHUANG ; Xiaoxia WU ; Dongfang YIN ; Lifang LIU ; Jia LIU
Organ Transplantation 2020;11(2):311-
Renal transplantation is one of the most effective treatment methods for end-stage renal diseases. However, some recipients present with fatigue symptoms after renal transplantation. Fatigue not only affects the quality of life, but also reduces the compliance of recipients with immunosuppressive agents. To strengthen the attention of medical staff to the fatigue, make early diagnosis and deliver effective interventions for renal transplant recipients, the current situation, risk factors and intervention methods of fatigue in renal transplant recipients were reviewed in this article.
10. Analysis of influencing factors of subjective well-being of gynecologic tumor patients
Xing LIU ; Derong LONG ; Jing CHEN ; Dongfang FU ; Meng LI ; Yue WANG ; Li ZHOU
Chinese Journal of Practical Nursing 2018;34(20):1552-1556
Objective:
To investigate the subjective well-being of gynecologic tumor patients and analyze the influencing factors.
Methods:
Investigated a total of 411 gynecologic tumor patients by using the a general information questionnaire, Memorial University of Newfoundland Scale of Happiness (MUNSH), Social Support Rating Scale(SSRS) and Medical Coping Modes Questionnaire(MCMQ).
Results:
The total scores of SWB was (32.20±9.54) points, at a moderate level. The influencing factors of subjective well-being of gynecologic tumor patients included: type of tumor, complication, education, the way of payment for medical treatment, average income per person in family, social support and medical coping modes. Furtherly, average income per person in family, social support and the resigntion dimension of medical coping modes were the main factors.
Conclusions
The SWB of gynecologic tumor patients need to be improved. To increase the SWB of the patients, the family, society and medical staff should give them more emotional, informational and other kinds of support, and try to minimize negative coping modes such as resigntion.