1.Effect of Acupuncture on Estrogen,Estrogen Receptor and Estrogen Signal Transduction in Rat Model of Hyperplasia of Mammary Glands
Dongfang GONG ; Juan XIE ; Chunzhi TANG
Journal of Guangzhou University of Traditional Chinese Medicine 2004;0(05):-
0.05).After treatment,the nipple height was increased in the model group(P
2.Progress of Th17 cells in chronic rhinosinusitis.
Yang WANG ; Zhenzhen TANG ; Dongfang YAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2016;30(2):161-166
Chronic rhinosinusitis is a common disease and frequently encountered disease in otolaryngology, but the therapeutic effect is not ideal. While its pathogenesis is exploring in the continuously. Found in the recent years, Th17 cells are a new subset of T cells and closely related with inflammatory disorders, autoimmune diseases and cancer. Its differentiation, regulation and biological effects are widely noted as a hot area of research. This review explores the discovery of differentiation and regulation of Th17 cells, the relationship between related cytokines and chronic rhinosinusitis, in order to have a beteer knowledge of chronic rhinosinusitis. This review regards Th17 cells as the main clue, nevertheless, lacking consideration of the impacts of other factors on chronic rhinosinusitis.
Chronic Disease
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Humans
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Rhinitis
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immunology
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Sinusitis
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immunology
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Th17 Cells
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immunology
3.Analysis of syndrome discipline of generalized anxiety disorder using data mining techniques.
Qisheng TANG ; Wenjun SUN ; Miao QU ; Dongfang GUO
Journal of Integrative Medicine 2012;10(9):975-82
To study the use of data mining techniques in analyzing the syndrome discipline of generalized anxiety disorder (GAD).
4.Relationship between iron overloading and heme oxygenase-1 after rat intracerebral hemorrhage
Gaiqing WANG ; Qidong YANG ; Guanglai LI ; Qingping TANG ; Dongfang LI ; Xia LIAN ; Yuheng PEI ; Liansheng MA ; Yanqing HAN
Chinese Journal of Geriatrics 2008;27(5):380-384
Objective To investigate whether iron mass induces HO-1 overexpression and explore the role of HO-1 in rat intracerebral hemorrhage(ICH). Methods In this study,144 hydrated chloride aldehyde-anesthetized Sprague- Dawley rats were used,autologous blood were injected into the right caudate nucleus to establish the ICH model.Saline injection and health were served as controls.Deferoxamine(DFO)with an intraperitoneal injection served as intervention group.Enhanced Perl's reaction was used for iron staining and brain iron deposits were determined.Brain HO-1 level were examined by immunohistochemical analysis and reverse transcription polymerase chain reaction(RT-PCR). Results There was a 21-fold increase in iron deposits around the hematoma 7 days after the infusion of 100 μl of autologous blood.Markedly increased levels of perihematomal HO-1 immunoreactivity and HO-1 mRNA in all ICH rats were detected at 3-14 days.The addition of DFO significantly reduced iron deposits in the ipsilateral basal ganglia at 7-14 days after ICH.DFO also inhibited HO-1 overexpression at day 7,14.Correlations test showed that there were positive correlations of iron sediments with HO-1mRNA(r=0.647)and HO-1 immunopositive cells(r=0.209). Conclusions ICH causes iron accumulation in the brain.Iron overloading may induce HO-1 upregulation after ICH.Ratherly,the HO-1 moderate increasing possibly fits with the events,whereas HO-1 overexpression may result in its dysfunction.It may be prudent to intervene ICH with HO-1 inhibitor.
5.Genetic analysis of a pedigree affected with congenital high myopia caused by a novel splice site variant of COL11A1 gene.
Xiurong YU ; Yichu LIU ; Fenghua LAN ; Qingqin LI ; Ying TANG ; Zhihong WANG
Chinese Journal of Medical Genetics 2019;36(9):893-896
OBJECTIVE:
To analyze genetic variant in a pedigree affected with congenital high myopia.
METHODS:
Whole exome sequencing (WES) was carried out for the proband. Suspected variation was verified with Sanger sequencing. The pedigree was also subjected to co-segregation analysis.
RESULTS:
WES has identified a novel splice site heterozygous variant (c.2556+1G>A) in the COL11A1 gene in the proband. Co-segregation analysis of the pedigree showed that the affected mother and two daughters of the proband have carried the same variant(c.2556+1G>A), while his unaffected father and sister did not. Based on the ACMG Standards and Guidelines for the Interpretation of Sequence Variants, the variant was classified as "likely pathogenic" (PVS1+PM2).
CONCLUSION
A novel splice variant (c.2556+1G>A) of the COL11A1 gene has been identified in a pedigree affected with congenital high myopia, which probably underlies the disease.
Collagen Type XI
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genetics
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Genetic Testing
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Heterozygote
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Humans
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Myopia
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genetics
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Pedigree
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Whole Exome Sequencing
6.Effect of preoperative pulmonary artery pressure on perioperative prognosis of recipients with end-stage heart failure undergoing heart transplantation
Wei ZHOU ; Bailing LI ; Guanxin ZHANG ; Yangfeng TANG ; Shaolin MA ; Daoxi HU ; Xingli FAN ; Lin HAN
Organ Transplantation 2023;14(1):100-
Objective To evaluate the effect of preoperative pulmonary artery pressure on perioperative prognosis of the recipients with end-stage heart failure undergoing heart transplantation. Methods Clinical data of 105 recipients receiving heart transplantation were retrospectively analyzed. The mean pulmonary artery pressure (mPAP) was used as the diagnostic criterion. The optimal cut-off value of mPAP for predicting perioperative prognosis of heart transplant recipients was determined. According to the optimal cut-off value of mPAP, all recipients were divided into the low mPAP group (
8.Serum metabolic profiling of traditional Chinese medicine syndromes in patients with diarrhea-predominant irritable bowel syndrome.
Si-Qi TANG ; Yun-Liang WANG ; Zi-Ye XIE ; Yang ZHANG ; Yi GUO ; Kang-Li GAO ; Tang-You MAO ; Chun-E XIE ; Jun-Xiang LI ; Xiao-Yan GAO
Journal of Integrative Medicine 2021;19(3):274-281
OBJECTIVE:
The clinical symptoms of diarrhea-predominant irritable bowel syndrome (IBS-D) can be effectively improved by traditional Chinese medicine (TCM) treatment, based on the usage of specific therapies for different TCM syndromes. However, in the stage of diagnosis, the standard criteria for the classification of TCM syndrome were still deficient. Through serum metabolic profiling, this study aimed to explore potential biomarkers in IBS-D patients with different TCM syndromes, which can assist in diagnosis of the disease.
METHODS:
Serum samples were collected from healthy controls (30 cases), IBS-D patients with Liver-Stagnation and Spleen-Deficiency syndrome (LSSD, 30 cases), Yang Deficiency of Spleen and Kidney syndrome (YDSK, 11 cases) and Damp Abundance due to Spleen-Deficiency syndrome (DASD, 22 cases). Serum metabolic profiling was conducted by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The potential biomarkers were screened by orthogonal partial least square-discriminate analysis, while metabolic pathways undergoing alterations were identified by pathway enrichment analysis in MetaboAnalyst 4.0.
RESULTS:
Overall, 34 potential biomarkers were identified in LSSD group, 36 in YDSK group and 31 in DASD group. And the 13 metabolites shared by three groups were determined as the potential biomarkers of IBS-D. Glycerophospholipid metabolism was disturbed significantly in IBS-D patients, which may play a role in IBS-D through inflammation. What's more, three TCM syndromes have the specific potential biomarkers in glycerophospholipid metabolism.
CONCLUSION
The serum metabolomics revealed that different TCM syndrome types in IBS-D may have different metabolic patterns during disease progression and glycerophospholipid metabolism was one of the pathways, whose metabolism was disturbed differently among three TCM syndromes in IBS-D. Therefore, the specific potential biomarkers in glycerophospholipid metabolism of three TCM syndromes in IBS-D can serve as the objective indicators, which can facilitate the TCM-syndrome objective classification of IBS-D.
9.Analysis of HEXB gene mutations in an infant with Sandhoff disease.
Ruohao WU ; Wenting TANG ; Kunyin QIU ; Yu LI ; Lirong LU ; Dongfang LI
Chinese Journal of Medical Genetics 2019;36(9):930-934
OBJECTIVE:
To detect potential mutations of HEXB gene in an infant with Sandhoff disease (SD).
METHODS:
Genomic DNA was extracted from peripheral blood sample of the infant. All coding exons (exons 1 to 14) and splicing sites of the HEXB gene were subjected to PCR amplification and direct sequencing.PubMed Protein BLAST system was employed to analyze cross-species conservation of the mutant amino acid. PubMed BLAST CD-search was performed to identify functional domains destroyed by thecandidate mutations. Impact of the mutations was analyzed with software including PolyPhen-2, Mutation Taster and SIFT. Whole-exome sequencing was carried out to identify additional mutations.
RESULTS:
The infant was found to carry compound heterozygous mutations c.1652G>A(p.Cys551Tyr) and c.1389C>G (p.Tyr463*) of the HEXB gene. The c.1389C>G (p.Tyr463*) mutation may lead to destruction of two functional domains in β subunit of the Hex protein. The c.1652G>A(p.Cys551Tyr) mutation, unreported previously,was predicted to be probably damaging by Bioinformatic analysis.
CONCLUSION
Compound heterozygous mutations c.1652G>A(p.Cys551Tyr) and c.1389C>G (p.Tyr463*) in the HEXB gene probably underlie the disease in this patient.
DNA Mutational Analysis
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Exons
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Heterozygote
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Humans
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Infant
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Mutation
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Polymerase Chain Reaction
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Sandhoff Disease
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genetics
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beta-Hexosaminidase beta Chain
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genetics
10.Clinical and genetic analysis of a family with autosomal dominant-familial Mediterranean fever.
Dongfang LI ; Wenting TANG ; Kunyin QIU ; Liangwu PAN ; Xiaojuan LI ; Ruohao WU
Chinese Journal of Medical Genetics 2021;38(8):719-722
OBJECTIVE:
To analyze a pathogenic variant of MEFV gene in a family with autosomal dominant-familial Mediterranean fever (AD-FMF).
METHODS:
A 5-year-old boy presented with recurrent aseptic meningitis and his major symptoms included recurrent fever with headache and vomiting. His family members including his mother, sister and brother also had recurrent fever. A genetic disease was considered. DNAs were extracted from patient and all his family members' blood samples. Whole exome sequencing was performed to identify putative pathogenic variants that can explain this family's condition and Sanger sequencing was conducted. The impact of detected variants were predicted and validated by bioinformatics.
RESULTS:
A missense variant c.2229C>G (p.Phe743Leu) in MEFV gene was identified in the proband and his family members including his mother, sister and brother. This variant had not been reported in China previously, but the locus of it had already been reported in Arabic patient with AD-FMF (PS1). This variant was absent in major allele frequency databases (PM2) and had been predicted to be pathogenic based on Mutationtaster, PROVEAN and PolyPhen-2. In addition, the change of amino acid, locating in 743 locus of pyrin protein, encoding by MEFV gene, was found to cause SPRY_PRY_TRIM20 and SPRY_superfamily domain destroyed and finally influenced the fuction of pyrin protein. On the other hand, using UCSF chimera software, we find the variant c.2229C>G (p.Phe743Leu) can induce serious influence to the spatial structure of pyrin protein and loss of protein fuction (PP3). According to the ACMG variant classification guideline, the variant c.2229C>G (p.Phe743Leu) in MEFV gene was classified as likely pathogenic (PS1+PM2+PP3).
CONCLUSION
The condition of this AD-FMF family may be attributed to the missense variant c.2229C>G (p.Phe743Leu) in MEFV gene. The recurrent aseptic meningitis was a very rare manifestation in AD-FMF patients and had not been reported in China previously. The clinical and genetic findings of the present study are helpful for the further understanding of AD-FMF.
Child, Preschool
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Familial Mediterranean Fever/genetics*
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Gene Frequency
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Genetic Testing
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Humans
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Male
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Mutation
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Pyrin/genetics*
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Whole Exome Sequencing