Aim To investigate the effects of breviscapine(Bre)on Na+,K+-ATPase activity of proximal tubule(PT) and its renal protection in early diabetic rats. Methods The rats were divided into three groups: diabetic model (DM group), Bre treatment (Bre group) and normal control (NC group). Rats were administrated with Bre(20 mg?kg-1?d-1, ip) in Bre group, with normal saline(ip) in DM and NC groups for four weeks after diabetes induction with streptozotocin (STZ 65 mg?kg-1, ip) in Bre and DM groups. The urine and blood samples were collected from two intra-ureteral cannulas and the heart, respectively, under anesthesia four weeks after diabetes induction. After one of the renal arteries being perfused, the renal cortex was incubated and PT segments were microdissected freehand under microscope before the Na+,K+-ATPase activity in the segments were assessed by liquid scintillation counter. The blood glucose, levels of creatinine in serum and in urine were assayed. The microalbumin , ?2-microglobulin(?2-MG) of urine and endogenous digitalis-like substance (EDLS) of serum were measured respectively by radioimmunoassay. Results The PT Na+,K+-ATPase activity in NC group was(959.11?117.35) pmolPi?mm-1?h-1, and that in DM group was significant higher for (1893.53?383.90) pmolPi?mm-1?h-1 than it(P

This study was aimed to select the best preparation form of medicinal in camellia cake,based on the optimal antithrombotic agents in oil tea camellia defatted cake to establish the foundation of research.The content of effective index component of the oil tea camellia defatted cake preparation was detected by HPLC-PDA.The quality of medicinal preparation from oil tea camellia defatted cake was characterized based on the content,relative quantity,relative ratio of content,yield rate and antithrombotic effect.The results showed that the effect of antithrombotic of three medicinal preparations from oil tea camellia defatted cake was listed in the following descending order of enrichment,ethanol extract,and water extract.The enrichment was the best preparation form of medicinal in oil tea camellia defatted cake preparation with the highest content of effective index components,the lowest extract rate and the lowest dosage.But it had the best effect of antithrombotic.It was concluded that the significant improvement of efficacy was due to the increase of the content of the components in the preparation and the rationality of the proportion.At the same time,analysis of component quality based on basic composition of drug system through the consideration of effective components of type and quantity,composition and other quality factors can further guide the preparation of drugs.

0.05).Conclusion HV-CVVH is effective in the treatment of severe acute pancreatitis.For SAP patients who are suit for the hemofiltration treatment,the treatment of HV-CVVH for 8h and for more than 8h has nearly the same effectiveness.

Objective. To study the role of endogenous carbon monoxide (CO) in endotoxin shock. Methods. The changes of CO levels and the effects of zinc protoporphyrin IX (ZnPP),an inhibitor of heme-oxygenase (HO), in endotoxin shock and the efficacy of hemin,an inducer of HO were investigated.Results. The plasma CO levels were found to be significantly increased during the course of endotoxin shock. Injection of ZnPP was shown to abrogate the endotoxin-induced hypotension and metabolic derangements markedly. Administration of hemin to healthy rabbits revealed the hypotension and metabolic derangements similar to the animals given endotoxin.Conclusion.CO is a newly found endogenously produced mediator which may play an important role in the pathogenesis of endotoxin shock.

China has a heavy burden of hepatocellular carcinoma, which is a serious threat to people′s life and health. However, the available drugs for advanced hepatocellular carcinoma in the past are limited and the efficacy is not satisfactory. In recent years, immunotherapy has a significant effects in some tumors. The authors introduce the efficacy of restart immunotherapy on an advanced hepatocellular carcinoma patient undergoing interruption of treatment due to corona virus disease 2019, in order to provide references for the diagnosis and treatment of this kind of patients.

In this study, we established a rapid and efficient HPLC method to determine the accumulation of Huperzine A and Huperzine B in the fermentation broth of endophytic fungus Colletotrichum gloesporioides from Huperzia serrate. The chloroform extracts of fermentation broth were dissolved in methanol and filtered before injection for HPLC analysis. The analysis was performed on an Agilent Eclipse plus-C18 column (250 mm×4.6 mm, 5 μm) by isocratic elution. The mobile phase was 0.015 mol/L ammonium acetate-methanol (70:30, V/V), the flow rate was 1 mL/min and the detection wavelength was set at 308 nm. Huperzine A and Huperzine B could be well separated within 25 min. Good linearity of Huperzine A was found in the range of 1.50-48.00 μg/mL (r=0.999 5), and that of huperzine B was in 0.25-7.50 μg/mL (r=0.999 7). The average recoveries of Huperzine A and Huperzine B were 106.83% and 108.06%, respectively (RSD=3.34%, 3.60%). The results demonstrate that this method can detect the content of huperzine A and huperzine B in fermentation broth simply, rapidly, accurately and in good reproducibility. Under the optimized conditions, the accumulated content of huperzine A and huperzine B were measured from the sixth to the fifteenth day. Huperzine A and Huperzine B reached the highest (12.417 0 μg/mL and 4.660 3 μg/mL, respectively) at the fourteenth and eighth days. The analysis methodology could contribute to the future study of huperzine A and huperzine B biosynthesis in C. gloeosporioides, consequently facilitate the development of new drug resources.

Objective To establish quality representation and correlation analysis method based on the spectrum of medicinal system of Shanzha(Fructus Crataegi,Chinese hawthorn fruit),so as to evaluate quality of Shanzha decocting pieces effectively and accurately.Methods HPLC-PDA method was ap-plied to characterize quality of spectrum of Shanzha medicinal system;quality of 11 batches of Shanzha decocting pieces were characterized based on the number and chemical type of peaks on characteristic spectrum.The content of characteristic index components,protocatechuic acid,epicatechin,chlorogeni-cacid,phenolic acids (represented by protocatechuic acid and chlorogenic acid)and flavonoids (repre-sented by hyperoside and epicatechin)were characterized by indexes in characteristic spectrum.Correla-tion analysis of quality and quantity of Shanzha was then performed with reference to the standard refer-ence pieces.Results As the reference substance,characteristic spectrum of batch 1 contains 13 charac-teristic peaks,7 peaks of flavonoids and 6 peaks of phenolic acids.All characteristic peaks appeared on the chromatograms of 11 batches of Shanzha.Batch 5,10,8,2,6 had more effective index ingredients. After comprehensive evaluation,there was a high correlation between batch 6,4,3,7,8,2 and standard reference pieces.The quality of batch 5,10,8,2,7,and 3 ranked on the top among all samples.Con-clusion HPLC method to characterize quality and quantity of characteristic spectrum of 11 batches of Shanzha is simple and accurate.The evaluation mode of quantity and quality and their correlation based on Shanzha medical system,including systemic correlation and application validity,can evaluate the quality of hawthorn pieces effectively and accurately.

Traumatic rib fractures are the most common injury in thoracic trauma. Previously，the patients with traumatic rib fractures were mostly treated non-surgically，of which 50%，especially those combined with flail chest presented chronic pain or chest wall deformities and over 30% had long-term disabilities，being unable to retain a full-time job. In the past two decades，thanks to the development of internal fixation material technology，the surgical treatment of rib fractures has achieved good outcomes. However，there are still some problems in clinical treatment，including inconsistency in surgical treatment and quality control in medical services. The current consensuses on the management of regional traumatic rib fractures published at home and abroad mainly focus on the guidance of the overall treatment decisions and plans，and relevant clinical guidelines abroad lacks progress in surgical treatment of rib fractures in recent years. Therefore，the Chinese Society of Traumatology affiliated to Chinese Medical Association and Chinese College of Trauma Surgeons affiliated to Chinese Medical Doctor Association，in conjunction with national multidisciplinary experts，formulate the Chinese Consensus for Surgical Treatment of Traumatic Rib Fractures（2021）following the principle of evidence-based medicine，scientific nature and practicality. This expert consensus puts forward some clear，applicable，and graded recommendations from aspects of preoperative imaging evaluation，surgical indications，timing of surgery，surgical methods，rib fracture sites for surgical fixation，internal fixation methods and material selections，treatment of combined injuries in rib fractures，in order to provide references for surgical treatment of traumatic rib fractures.