Objective To explore the clinical significance of serum total bile acid (TBA) test in evaluation of the prognosis of posthepatitis cirrhosis.Methods 92 patients with posthepatitis cirrhosis were selected as the observation group,and divided into A,B,C groups according to the Child-Pugh grades.32 healthy people were selected as the control group.The level of serum TBA was detected and compared.Results The level of TBA in the observation group was (84.26 ±49.03) μmol/L,which was higher than that in the control group[(3.52 ± 2.67) μmol/L](t =17.53,P ＜ 0.01).The level of TBA was gradually increased in Child-Pugh grade A,B,C grade,and the differences were statistically significant (t =3.84,6.21,5.49,all P ＜ 0.05).Conclusion TBA test is benefit for the diagnosis and prognosis of posthepatitis cirrhosis.

Objective To investigate the correlation between drug-resistant genes and mobile genetic elements in multi-drug resistant Escherichia coli,and to explore phylogeny among the strains.MethodsTotally 20 strains of multi-drug resistant Escherichia coli were collected from Pan' an Hospital,Zhejiang Province during June 2009 and June 2010.Beta-lactam-resistance genes,aminoglycoside-resistance genes,genetic markers of mobile genetic elements were analyzed by PCR.Index and sample cluster analysis were performed on above results. Results In 20 strains of Escherichia coli,4 kinds of beta-lactamresistance genes,4 kinds of aminoglycoside-resistance genes,and 5 kinds of genetic markers of mobile genetic elements were detected.Index cluster analysis showed that correlation existed between resistance genes TEM,CTX-M-1,aadA5 and mobile genetic elements traA,IS26,ISEcpl; and correlation also existed between resistance genes OXA-1,aac(6′)-Ⅰ b,ant(3)-Ⅰ,rmtB and mobile genetic elements trbC,IS903.Sample cluster analysis showed that this group of Escherichia coli could be divided into 2 groups which were genetically different.ConclusionsDrug-resistant genes in multi-drug resistant Escherichia coli are correlated with mobile genetic elements.Sample cluster analysis can reveal phylogeny among the strains,which is important for hospital infection control.