OBJECTIVE To strengthen the hospital supply Division standardized management and prevent the pathogenic microorganism dissemination effectively and safeguard the human health.METHODS Strengthening the vocational study,improving personnel′s knowledge level,formulating control system,strengthening detail and flow management and adopting the effective monitoring method all were the main measures.RESULTS The results were to guarantee the aseptic material quality,and prevent the occurreance of hospital Ainfection.CONCLUSIONS The scientific and effective management measures make our supply division a good quality management institution,with the programming the materiol processing and the standardizing the disinfection and antisepsis.

Abortifacient Agents, Nonsteroidal ; pharmacology ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Female ; Pinellia ; chemistry ; Plant Proteins ; pharmacology ; Pregnancy

Objective:To study the expression and clinical significance of GPRC5A and SOCS3 in colorectal carcinoma.Methods:SP immunochemical method was performed to detect the expression of GPRCSA and SOCS3 in 45 cases of colorectal carcinoma,25 cases of colorectal adenomas and 22 cases of normal colorectal tissues.Results:1)Expression of GPRC5A in colorectal cancinoma tissue (22.2％) was significantly lower than that in adenomas tissue (52.0％,P＞0.05).The Latter was significantly lower than that in normal colorectal tissue (81.8％,P＜0.05).GPRC5Awas closely related to lymph node metastasis,Duke's stages and the deepness of invasion (P＜0.05).2) Expression of SOCS3 in colorectal cancinoma tissue (24.4％) was significantly lower than that in adenomas tissue (56.0％,P＜0.01).The Latter was significantly lower than that in normal colorectal tissue (86.4％,P＜0.05).SOCS3 was closely related to pathological differentiation,the deepness of invasion,Duke's stages and lymph node metastasis (P＜0.05).3)The expression of GPRC5A was positive correlated with SOCS3 (P＜0.05).Conclusions:The reduced expressions of GPRC5A and SOCS3 may participate in the occurence and development of colorectal carcinorma,suggesting that GPRC5A and SOCS3 may act as biological markers for evaluating the malign degree,prognosis and therapeutic targets of colorectal carcinorma.

Objective: To evaluate the impact of the number of negative lymph nodes on disease free survival (DFS) of patients with locally advanced gastric cancer. Methods: A total of 485 patients who underwent surgery for gastric cancer were enrolled in this study. We analyzed the data of these patients including complete information of follow-up and definite postoperative pathological results. The disease free survival of all patients was more than 6 months and was analyzed according to the pathological stage respectively. COX regression analysis was performed to screen the independent prognostic factors. Kaplan-Meier method was used to draw the disease free survival curve and the disease free survival rate was compared among subgroups. Results: Cox regression analysis showed that the disease free survival rate of gastric cancer patients with pathological stage T_3N_(0～2)M_0 was significantly associated with age, degree of tumor differentiation, tumor location as well as the number of negative lymph nodes. In the T_3N_0M_0 subgroup, when the number of negative lymph nodes was 1 to 4 and 5 or more, the 2-year disease free survival rate was 8.3% and 55.6%, respectively, and the 3-year disease free survival rate was 0 and 24.9%, respectively (P=0.025).In the T_3N_1M_0 subgroup, when the number of negative lymph nodes was 3 or less, 4 to 9, and 10 or more, the 2-year disease free survival rate was 17.3%, 39.1%, and 52.6%, respectively, and the 3-year disease free survival rate was 4.2%, 6.0%, and 17.1%, respectively (P<0.001). In the T_3N_1M_0 subgroup, when the number of negative lymph nodes was 7 or less and 8 or more, the 2-year disease free survival rate was 11.5% and 35%, respectively, and the 3-year disease free survival rate was 0.8% and 5%, respectively (P=0.015).Conclusion: For gastric cancer patients with pathological stage T_3N_(0～2)M_0, the number of negative nodes could reflect the level of regional lymph node dissection. The number of negative nodes can be an index for evaluation of the accuracy of pathological staging and is considered as an independent prognostic factor for disease free survival of gastric cancer patients.

Objective To investigate the infection of human papilloma virus(HPV) and expression of serum squamous cell antigen (SCCAg) in the follow - up of 120 cervical squamous carcinoma patients who had received operation or operation combined with radiotherapy or only radiotherapy. Methods 120 cases of therapical cervical squamous carcinoma patients were detected HPV - DNA by HC - Ⅱ and serum SCCAg using immunohistochemical method. Results The positive rate of HPV - DNA and serum SCCAg was 49.17% and 17.50% , with a significant difference between them( P 0.05 ). Conclusion HPV - DNA test with HC - Ⅱ for follow - up of cervical squamous carcinoma patients was feasible. It was more sensitive than serum SCCAg. But it suggested that high risk type HPV -DNA test combined with serum SCCAg may be the independent prognostic factors.

Objective The current study was to investigate the clinical and pathological features of macroscopic hematuria associated acute kideny injury (MH-AKI) in patients with IgA nephropathy (IgAN) and its associated prognostic factors.Methods We performed an analysis on the clinical and pathological features of biopsy-proven IgAN patients diagnosed during 2004 to August of 2007,who had experienced MH associated AKI.The patients enrolled in the study were followed up.The renal lesions were evaluated with Katafuchi pathological scores of IgAN.Results 5 patients with IgAN had experienced at least one episode of MH-AKI,accounting for 1.3% of IgAN diagnosed during the same period and 13.5% of IgAN patients with macroscopic hematuria.High proportion of tubules filled with red blood cell casts and tubular injury were demonstrated in all biopsy specimens.Renal function of 3 patients completely recovered within 14 days.One patient (patient 4) got quick recovery in the first month of the disease but completely recovered 418 days later.The other patients only had an incomplete recovery during the 20-month follow-up.The incompletely recovered one had relatively an older age,longer persistence of MH,more sclerotic glomeruli and a more severe tubule-interstitial damage.Conclusion AKI is commonly seen during episodes of MH in patients with IgAN.Not all patients with MH-AKI showed a complete recovery.Age,duration of MH,proportion of glomerular sclerosis and the severity of tubular necrosis might be the risk factors of an incomplete recovery of renal function.

Objective To investigate the possible mechanisms of teratogenesis caused by passive smoking, temporospatial changes in surface carbohydrates of the embryonic neural epithelium and the mesenchyme around it were explored. Methods The pregnant golden hamsters were randomly divided into the treated group and the control groups. In the chamber made by ourself for passive smoking experiment, the animals of the treated group were exposed to smoke from embryonic day 4 to 7, 3 times per day for 4 consecutive days. At twelve o'clock and eighteen o'clock of embryonic day 8, eight o'clock of embryonic day 9 and eight o'clock of embryonic day 10 respectively, the pregnant golden hamsters of both treated group and control group were sacrificed, the uteri were removed and embedded routinely with paraffin, sectioned transversely in series. The sections of each group were stained with immunohistochemical ABC method and concannavalin agglutinin (ConA) and ulex europaeus agglutinin (UEA) were used as molecule probes to conduct the qualitative and quantitative analysis of the fibronectin and the related surface carbohydrates of the embryonic neural epithelium and the mesenchyme around it. Results During embryonic day 8 to10 of golden hamsters fibronectin reduced(F=30.168,P

Objective To study the relationship of lung function and the diaphragmatic nerve electrophysiological changes in elderly patients with chronic obstructive pulmonary disease(COPD).Methods The phrenic nerve motor conduction(PNC),diaphragmatic motor evoked potentials(dMEP)and diaphragmatic compound muscle action potentials(dCMAP)were detected in 50 COPD patients and 50 healthy controls aged 60 years and over,respectively. Results (1)The lung function of elderly COPD patients were significantly less than that of control group(P0.05),the lower amplitude of dCMAP than the control(P