1.Construction and transfection of neural stem cells of recombinant plasmid pLEGFP-mIFN?
Journal of Jilin University(Medicine Edition) 2006;0(04):-
Objective To construct a recombinant plasmid pLEGFP-mIFN? for developing the genetical therapy of tumor by stem cells.Methods PCR was used to amplify the mIFN? gene from plasmid pcDNA3.1-mIFN? in which the mIFN? was cloned.A new plasmid,pLEGFP-mIFN?,was then constructed by inserting the amplified mIFN? gene into pLEGFP-C1.Restriction analysis and sequencing were used to confirm the structure of pLEGFP-mIFN?.Neural stem cells were transfected by supernatant of PA317.Results A DNA fragment in size of 500 bp was amplified.Restriction analysis showed that the amplified gene was inserted into pLEGFP-C1 correctly.(Conclusion The) plasmid pLEGFP-mIFN? is constructed successfully,and it can transfect neural stem cells.
4.Function of type I repetitive consequence peptide segment of thrombospondin-1 in inhibition of fibrosis of mice.
Ying CHEN ; Xing WANG ; Dong WENG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(1):45-47
Animals
;
Disease Models, Animal
;
Female
;
Fibrosis
;
metabolism
;
pathology
;
Hydroxyproline
;
metabolism
;
Lung
;
drug effects
;
metabolism
;
pathology
;
Mice
;
Thrombospondin 1
;
pharmacology
5.Nitrogenous chemical constituents of Weiceng
Quanwei CHEN ; Ying TIAN ; Hengwen CHEN ; Ting GAO ; Junxing DONG
Military Medical Sciences 2014;(1):62-66
Objective To investigate the nitrogenous chemical constituents of Weiceng .Methods Weiceng extract was subjected to various column chromatography and spectroscopic methods were used for the elucidation of compounds .Results Seventeen compounds were isolated and identified as cyclo-(Leu-Tyr)(1), cyclo-(Phe-Tyr) (2), cyclo-(Pro-Gly) (3), L-Pyroglutamic acid methyl ester (4), uracil (5), thymine (6), N-acetylphenylalanine (7), tyrosine (8), phenylala-nine (9), phenylalanine methyl ester (10), N-methyl leucine (11), isoleucine (12), valine (13), leucine (14), glutamic acid (15), glycine (16) and aspartic acid (17).Conclusion All the seventeen compounds are isolated from Weiceng for the first time .Before this study , cyclopeptides 1-3 have never been isolated from soy bean or its products .
6.The study on effect of long-termed administration of mixed rare earth Changle on rat liver
Ying LIU ; Dong CHEN ; Aijun CHEN ; Xiaoming WANG ; Yuxiu NIE
Journal of Jilin University(Medicine Edition) 2002;28(6):567-570
Objective: To research effect of different doses of Changle on the structure and function of rat liver.Methods: 180 healthy Wistar rats were divided into 5 experimental groups according following doses: 0.1,0.2,2.0,10.0 and 20.0 mg*kg-1,respectively, and the control group given physiological saline for six months. The changes of liver structure were examined by means of normal histological chemistry and transmission electron microscope(TEM). Results: The body weight of animal was linearly increased with the decrease of administered doses, gradual reduction of glycogen in hepatocytes and infiltration of inflammatory cells in the portal area were found in the group of 20.0 mg*kg-1. Changes of ultrastructure showed there were dense bodies and lysosomes containing dense granules in Kupffer cell and hepatocyte,and they were increased along with doses adding. Nuclei deformed, ALP and GPT in serum were rose in the group of 20.0 mg*kg-1. Different doses of Changle could lead to distinct biological effects. Conclusion: Long-termed administration of 20.0 mg*kg-1 Changle can lead to damage of structure and function of rat liver.
9.Effect of injection Salvia miltiorrhizae composite on colony-forming unit-erythroid and burst forming unit-erythro production in bone marrow of mice with the 60Co-gamma radiation damage.
Jing TAO ; Wei-Dong YANG ; Gui-Ying CHEN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(6):357-358
10.The clinical analysis of tension-free hernia repair in inguinal emergency hernia
Dong-Ming NA ; Jie CHEN ; Ying-Mo SHEN ;
Chinese Journal of Primary Medicine and Pharmacy 2005;0(11):-
Objective To study the clinical effect of tension-free hernia repair in inguinal emergency hernia. Methods The tension-free hernia repair was administered in 83 patients with inguinal emergency hernia.All the cases were analyzed retrospectively.Results There were two postoperative deaths in 83 cases within three days after operation.Wound infection occurred in one case.The rest recovered soon.The average stay in hospital was 5.1 days. Conclusion The tension-free hernia repair can be applied to inguinal emergency hernia with satisfactory results.