1.The study on the field environment suitability of multi-parameter patient monitor
China Medical Equipment 2015;(4):12-14
Objective:To study the environment suitability of multi-parameter patient monitor. Methods: Choosing six different types of multi-parameter patient monitor from different manufactures, we tested six main parameters of the multi-parameter patient monitor when they had gone through the environmental tests, and the test limits come from field environment. Results:Each of the samples was not up to the standards. Conclusion:At present a clear most of the multi-parameter patient monitor in the market are designed and produced for using indoors, which are not suitable for using in field environment. We need to enhance the protection of the multi-parameter patient monitor against field environment, and improve the environment suitability.
2.Analysis of the Effects of Biotechnology on Human Bio-safety and Ethics
Dong WU ; Shuxia WU ; Lei WANG
Chinese Medical Ethics 1996;0(01):-
Bio-safety problem was emerging as more and more important with the development of biotechnology,and many governments concerned about it.We analyzed the different category of biotechnology related with bio-safety problem,and found out its main influencing factors and safety results.In the course of research and development of biotechnology,misuse,abuse and non-moral use are three main human factors.We concluded that the situation of bio-threat would be more diverse,and also the misuse chance would increase.It will be benefited that public health manager make regulations and legislature with full dimension of bio-safety assessment of different biotechnologies.
3.Case of intractable chest pain with back pain.
Yanfen DONG ; Zhaohui WU ; Xiaolin WANG
Chinese Acupuncture & Moxibustion 2015;35(10):1043-1043
Acupuncture Therapy
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Adult
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Back Pain
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therapy
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Chest Pain
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therapy
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Female
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Humans
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Pain, Intractable
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therapy
4.Comparison of periphery capillary whole blood glucose using four kinds of blood glucose meters and venous plasma glucose using laboratory auto-analyzer
Nannan WU ; Meng WANG ; Dong ZHAO
Chinese Journal of Primary Medicine and Pharmacy 2015;22(4):582-585
Objective To evaluate the precision of the ACCU-CHEK Performa glucose monitor,the Optium Xceed glucose monitor,the Breeze glucose monitor and the Contour glucose monitor.Methods A total of 102 patients (diabetic patients or un-diabetic patients) who visited our endocrinology outpatient department were randomly selected.The venous blood samples were collected using the laboratory auto-analyzer,and the synchronous finger tip capillary blood were collected using the ACCU-CHEK Performa glucose monitor and the Optium Xceed glucose monitor,or using the Breeze glucose monitor and the Contour glucose monitor.Results The correlation coefficients between VPG by the laboratory auto-analyzer and CBG by the four kinds of glucose monitors was good,and R values were 0.990,0.985,0.963,0.952,and there was no significant difference between the CBG of Breeze glucose monitor and the same VPG(P > 0.05).When the blood glucose concentration was higher than 4.2mmol/L,and compared with the VPG detected by synchronous measurement laboratory,95% of the CBG test results detected by four kinds of blood glucose meters were in the range of ± 20%.Conclusion The four kinds of glucose monitors can provide high accurate.
5.Expression and clinical significance of estrogen receptor and progesterone receptor in endometrial carcinoma in aged patients
Xiuyan WANG ; Hongxia WU ; Yan DONG
Chinese Journal of Biochemical Pharmaceutics 2015;37(5):156-158,161
Objective To investigate expression of elderly patients with endometrial cancer of estrogen receptor and progesterone receptor and its clinical significance.Methods 45 elderly patients with endometrial carcinoma from March 2012 to June 2014 were selected as the observation group, over the same period healthy women with normal endometrium as control group, using S-P immunohistochemical method, to detect the expression of receptor and progesterone receptor in two groups.Results In observation group, estrogen and progesterone receptor positive expression rates were 60.00%, 46.67%, compared with control group(93.33%, 86.67%), was significantly lower (P<0.05).Patients with low differentiation of estrogen and progesterone receptor, positive expression rate was 37.50%, 25.00%, was lower than other two groups, but which had no difference.Estrogen, progesterone receptor positive expression rates in progress stage were 35.71%, compared with patients ( 80.65%, 74.19%) in early stage, was significantly lower (P<0.05).The positive expression rate of estrogen receptor in patients with lymph node metastasis was 66.67%, compared with the patients with no metastasis, the difference was not statistically significant.The positive expression rate of progesterone receptor was 76.67%, compared with the non metastasis group (46.67%), and was significantly lower (P<0.05).Conclusion The expression of estrogen and progesterone can reflect the biological behavior and prognosis of endometrial carcinoma in aged patients, and have important significance for clinical diagnosis and treatment.
7.The protective effect N-acetyl-L-cysteine against reperfusion injury on donor rat lung
Dong WANG ; Qingyu WU ; Xingpeng CHEN
Chinese Journal of Thoracic and Cardiovascular Surgery 2003;0(01):-
Objective To evaluate the protective effect by inhibiting nuclear factor-kappa B (NF-?B) by N-acetyl-L-cysteine(NAC) on donor rat lung. Methods 24 SD rats were randomly divided into two groups, the control group and the experiment group. The harvested lung blocks were flushed with and stored in the low-potassium-dextran (LPD) solution in control group, but in the experiment group, LPD solution containing NAC was used. The lungs were preserved at 4℃ for 16 hours in both groups. Isolated rat lung reperfusion models were established and the donor lungs were perfused for 1 hour. PaO_2 and PAwP were measured at every 15 min intervals during reperfusion. After reperfusion, the lung tissue wet-to-dry (W/D) ratio and myeloperoxidase(MPO) activity were obtained. The protein and mRNA expressions of intercellular adhesion molecule-1(ICAM-1) and nuclear factor-kappa B(NF-?B) were also observed by using immunohistochemistry and semi-quantitative RT-PCR at the end of reperfusion. Results After reperfusion for 60 minutes, in experiment group, PaO_2 drop in and increase PAwP lower than in control group (P
8.Morphologic and chromatic parameters of erythrocytes infected with Plasmodium vivax in thin peripheral blood smears
Dong WANG ; Wei WU ; Jianru YANG
Chinese Journal of Schistosomiasis Control 1989;0(04):-
Objective To explore the digital morphological and chromatic characteristics of erythrocytes infected with Plasmodium vivax. Methods The images of both normal and P. vivax infected erythrocytes were segmented and measured for morphologic parameters including area, length, breadth, perimeter, roundness, aspect ratio, equivalent circle diameter, as well as chromatic parameters including saturation and color (red, green, blue). A statistic analysis was performed for these parameters. Results Both morphological and chromatic parameters showed high significant differences between the normal and the infected erythrocytes, and high significant differences between the normal and the erythrocytes infected with different stages of P. vivax. Conclusion The differences mentioned above could be used as the basis for automatic identification of P.vivax in thin peripheral blood smears.
9.Regulation of MMP-2 and Cath-D by Interferons in Human Osteosarcoma Cell Line OS-732
Cuang WU ; Fengxun GAO ; Dong WANG
Chinese Journal of Orthopaedics 1999;0(07):-
Objective To explore the regulatory effects of IFNs on the expression of MMP - 2 andCath - D in osteosarcoma cell and provide experimental evidence for clinical treatment of osteosarcomametastasis. Methods The changes of MMP - 2 and Cath-D were half quantatively measured by Cell ELISA in a human osteosarcoma cell line OS - 732, treated with IFN - ? and IFN -?. Results The resultdemonstrated that both of IFNs were capable of significantly decreasing their expression (P
10.Effects of insulin on the DNA synthesis and cell cycle of human periodontal ligament cells cultured in vitro
Guangying DONG ; Zhifen WU ; Qintao WANG
Journal of Practical Stomatology 2001;0(03):-
Objective: To investigate the effects of insulin on the DNA synthesis and cell cycle distribution of human periodontal ligament cells (PDLs). Methods:PDLs were treated with insulin at the doses (U/L) of 0.01~1 000 for 72 h,DNA synthesis of the cells was measured by 3H-TdR incorporation and cell cycle distribution was studied by flow cytometer. Results : Insulin at 1 U/L to 1 000 U/L showed a significant concentration dependent stimulation of 3H thymidine uptake, and the effect of insulin at 100 ~ 1 000 U/L were not statistically different from that at 10 U/L. The percentage of PDL cells in G1 phase during exponential growth was decreased by insulin, while that in S phase and (G2+M) was increased in experimental group. Conclusion: Insulin increases DNA synthesis of PDL cells ,and stimulates the transformation of cells from G1 to S phase.