Objective:To study the environment suitability of multi-parameter patient monitor. Methods: Choosing six different types of multi-parameter patient monitor from different manufactures, we tested six main parameters of the multi-parameter patient monitor when they had gone through the environmental tests, and the test limits come from field environment. Results:Each of the samples was not up to the standards. Conclusion:At present a clear most of the multi-parameter patient monitor in the market are designed and produced for using indoors, which are not suitable for using in field environment. We need to enhance the protection of the multi-parameter patient monitor against field environment, and improve the environment suitability.

Bio-safety problem was emerging as more and more important with the development of biotechnology,and many governments concerned about it.We analyzed the different category of biotechnology related with bio-safety problem,and found out its main influencing factors and safety results.In the course of research and development of biotechnology,misuse,abuse and non-moral use are three main human factors.We concluded that the situation of bio-threat would be more diverse,and also the misuse chance would increase.It will be benefited that public health manager make regulations and legislature with full dimension of bio-safety assessment of different biotechnologies.

Acupuncture Therapy ; Adult ; Back Pain ; therapy ; Chest Pain ; therapy ; Female ; Humans ; Pain, Intractable ; therapy

Objective To explore the influential factors of the healthy lifestyle of college students, so as to provide basis for drafting the health education measure appropriate for college students. Methods 5400 college students received the assessment with the healthy lifestyle scale for college students to analyze the influential factors. Results Some factors affect the healthy lifestyles of college students, such as gender, temperament, self-rated health, self-rated achievement, type of college, grade, residential peer, birth region, educational levels of parents and family' s monthly-earning, and r -square for total score and each dimension of the healthy lifestyle of college students respectively were 0. 3202,0. 4173,0. 2104,0. 2247,0. 1936,0. 0922,0. 2013,0. 2181,0. 162. Conclusion Colleges should take different measures to stimulate student interest in taking health-promoting lifestyles based on their characteristics so as to develop healthy lifestyles during their college student life.

Objective To evaluate the precision of the ACCU-CHEK Performa glucose monitor,the Optium Xceed glucose monitor,the Breeze glucose monitor and the Contour glucose monitor.Methods A total of 102 patients (diabetic patients or un-diabetic patients) who visited our endocrinology outpatient department were randomly selected.The venous blood samples were collected using the laboratory auto-analyzer,and the synchronous finger tip capillary blood were collected using the ACCU-CHEK Performa glucose monitor and the Optium Xceed glucose monitor,or using the Breeze glucose monitor and the Contour glucose monitor.Results The correlation coefficients between VPG by the laboratory auto-analyzer and CBG by the four kinds of glucose monitors was good,and R values were 0.990,0.985,0.963,0.952,and there was no significant difference between the CBG of Breeze glucose monitor and the same VPG(P ＞ 0.05).When the blood glucose concentration was higher than 4.2mmol/L,and compared with the VPG detected by synchronous measurement laboratory,95％ of the CBG test results detected by four kinds of blood glucose meters were in the range of ± 20％.Conclusion The four kinds of glucose monitors can provide high accurate.

Objective:To investigate the effects of 17-? estradiol (E2) on the proliferation and metastasis of adenoid cystic carcinoma SAcc83 cells.Methods:The effects of E2 on the proliferation of SAcc83 cells were studied by MTT assay and flow cytometry.The invasion potential of the cells was investigated with chicken embryo heart invasion assay.The in vivo growth and metastasis of SAcc83 cell induced tumor were studied in ovary-resected female nude mice. Results:E2 at 10 -7 ,10 -8 and 10 -9 mol/L promoted the proliferation of SAcc83 cells. E2 at 10 -7 mol/L showed the strongest growth stimulation effects and increased the cell proliferation by 34.4%.With exposure time of 1,2 and 3 d it increased the proliferation index by 33.3%,40.9% and 17.8% respectively;with the exposure time of 12,18 and 24 h,it increased S phase cells in cell cycle by 12.3%,3.3% and 9.7% respectively. Neoplasm developed in all nude mice after subcutaneous transplantation of 106 cells.In the mice treated with 0.2 ml of E2 at 9.55 ?10 -5 mol/L every day for 15 days neoplasm volume doubling time was 61.5 h,that in control mice 76.5 h.In lung metastasis trial, 42 days after tail injection of the cells the metastasis foci in E2 treated mice inreased by 41.2%.Conclusion:E2 may increase the proliferation and metastasis of adenoid cystic carcinoma cells.

Objective:To study the effect of GnRH analog triptorelin in resensitizing cisplatin-resistant human ovarian canc- er cells and to discuss the related mechanism.Methods:Cisplatin-resistant human ovarian cancer cell line OVCAR-3/CDDP was established in vitro.MTT assay was used to assess the inhibitory effects of triptorelin,cisplatin alone or a combination of both on OVCAR-3/CDDP cells.Flow cytometry was employed to observe the expression changes in epidermal growth factor receptor (EGFR)in different groups.Results:The drug restant index of OVCAR-3/CDDP cells was 13.42.The resensitizing fold of cisplatin combined with triptorelin was 3.80.The expression of EGFR had the most prominent decrease in OVCAR-3/CDDP cells in the combination group.Conclusion:Triptorelin can partially resensitize cisplatin-resistant OVCAR-3/CDDP cells,which might be related to the down-regulation of EGFR.

Objective To evaluate the influence of tirofiban to percutaneous coronary intervention.Methods 66 elderly patients with coronary artery disease were treated,they were divided into the study group of 33 cases and the control group of 33 cases.The control group was treated with simple percutaneous coronary intervention treatment, the study group was treated with percutaneous coronary intervention combined with tirofiban.The changes of LVEF, cTnT,and TIMI,symptoms in the treatmeat and the symptoms after 2 years were observed and compared.Results After treatment,the patients'platelet count was to the basic line,the difference was not statistically significant(t =0.353 3,P >0.05).The cTnT,LVEF levels of the study group were significantly better than the control group,the differences were statistically significant(t =5.138 1,8.658 1,all P <0.05).After treatment,the incidence rate of TIM in both two groups were improved compared with before treatment.After treatment,the incidence rate of TIMI 3 of the study group(93.9%) was significantly higher than the control group(69.7%),the difference was statistically significant(χ2 =6.518 5,P <0.05).After treatment,the remission rate of angina of the study group(87.9%) was significantly higher than the control group(60.6%),the difference was statistically significant (χ2 =6.417 8,P <0.05).After 2 years of follow -up , the remission rate of angina and recurrent angina in the study group were significantly better than the control group,the differences were statistically significant (χ2 =5.076 9,3.995 2,all P <0.05).Conclusion The efficacy of percutaneous coronary intervention combined with tirofiban is significant,the prognosis is good,has higher remission,it is suitable for long -term clinical application.

Objective To evaluate the protective effect by inhibiting nuclear factor-kappa B (NF-?B) by N-acetyl-L-cysteine(NAC) on donor rat lung. Methods 24 SD rats were randomly divided into two groups, the control group and the experiment group. The harvested lung blocks were flushed with and stored in the low-potassium-dextran (LPD) solution in control group, but in the experiment group, LPD solution containing NAC was used. The lungs were preserved at 4℃ for 16 hours in both groups. Isolated rat lung reperfusion models were established and the donor lungs were perfused for 1 hour. PaO_2 and PAwP were measured at every 15 min intervals during reperfusion. After reperfusion, the lung tissue wet-to-dry (W/D) ratio and myeloperoxidase(MPO) activity were obtained. The protein and mRNA expressions of intercellular adhesion molecule-1(ICAM-1) and nuclear factor-kappa B(NF-?B) were also observed by using immunohistochemistry and semi-quantitative RT-PCR at the end of reperfusion. Results After reperfusion for 60 minutes, in experiment group, PaO_2 drop in and increase PAwP lower than in control group (P

Objective To observe the protective effect of dexamethasone, the inhibitor of nuclear factor-Kappa B, on the ischemia-perfusion injury of rat lung during the period of lung preservation. Methods Twenty-four rats were randomly divided into two groups: the control group and the trial group. The harvested lung blocks were flushed with and stored in the low-potassium-dextran (LPD) solution in control group, but in the trail group LPD containing dexamethasone solution was used. The lungs were stored at 4 ℃ for 16 h in both groups. The isolated rat lung reperfusion models were established and the donor lungs were perfused for 1 h. PaO_2 and PawP were measured at every 15 min intervals during reperfusion. After reperfusion, the lung tissue wet-to-dry (W/D) ratio and myeloperoxidase (MPO) activity were obtained. The protein and mRNA expression of intercellular adhesion molecule-1 (ICAM-1), nuclear factor-Kappa B (NF-?B) was also detected by using immunohistochemistry and semi-quantitative RT-PCR at the end of reperfusion. Results The levels of decreased PaO_2 and increased PawP in trail group were lower than in control group at the every interval time in the samples obtained 15 min after reperfusion (P