BACKGROUND: The method of replenishing kidney has becoming one of the therapies for anti-senility in gerontology in Chinese medicine.OBJECTIVE: To probe into the effects of the formula for nourishing kidney yin-zuoguiyin on serum malondialdehyde (MAD) content and superoxide dismutase (SOD) activity in metabolism of free radical.DESIGN: Complete randomized control experiment.SETTINGS: Microbiology and Immunology Department of Guangdong Medical College.PARTICIPANTS: The experiment was performed in Immunology Research Room of Wannan Medical College from September 2002 to July 2004. Forty male senile ICR mice were employed, provided by Institute of Experimental Animal of Medical Scientific Academy in Sichuan. They were randomized into senile control, zuoguiyin group, single shudi (Radix Rehmanniae Praeparata) group (single group) and shudi (Radix Rehmanniae Praeparata) removal group (removal group), 10 mice in each one. Zuoguiyin composes of shudi (Radix Rehmanniae Praeparata), shamyao (Rhizoma Dioscoreae), gouqi (Fructus Lycii), zhi gancao (Radix Glycyrrhizae Praeparata),fuling (Poria) and shangyurou (Fructus Corni) at the ratio of 9:6:6:3:4:5. After decocted routinely, the solution in zuoguiyin group was concentrated to 0.577 5 g/mL of raw herbs contained; that in single group was to 0.157 5 g/mL and that in removal group was to 0.42 g/mL. The mixed medical solutions of 100 g/L were prepared successively in each group.INTERVENTIONS: In zuoguiyin group, single group and removal group,solutions of zuoguiyin and shudi (Radix Rehmanniae Praeparata) and solution with shudi (Radix Rehmanniae Praeparata) removed were administrated successively, 4 times/day, 1.5 mL/time, totally for 75 days. No any drink was provided during the medication. The mice in senile control drank pipe water. After the eyeball extracted, blood of 2 mL collected and serum preserved, the thiobarbituric acid method was used to determine MAD content, the xanthine oxidase method was to determine SOD activity and the colorimetric method was to determine general capacity of anti-oxidation in serum.MAIN OUTCOME MEASURES: ① serum MAD content; ② serum SOD activity; ③ general capacity of anti-oxidation in serum.RESULTS: One mouse was dead in senile control and 3 mice in single group. Terminally, 36 mice entered result analysis. ① MAD content:Serum MAD content in zuoguiyin group and single group were lower than that in senile control [(5.280±1.204) μmol/L and (5.886 2.717) μmol/L,(9.533 3.494) μmol/L, (q=3.641-4.657, P ＜ 0.05)]. Serum MAD content in zuoguiyin group and single group were basically same. ② SOD activity: SOD activity in zuoguiyin group and removal group were higher than senile group [(7.008±0.782) mkat/L and (6.989±0.809) mkat/L, (5.908±0.392) mkat/L,(q=3.641-4.657, P ＜0.05)]. SOD activity in zuoguiyin group and removal group were basically same and that in single group and senile control were basically same. ③ General anti-oxidation capacity: The general capacity of anti-oxidation in serum in zuoguiyin group and single group were stronger than that in senile control, that in removal group was weaker than senile control, but the differences were not significant (P ＞ 0.05).CONCLUSION: Zuoguiyin remarkably reduces MAD content and improves SOD activity in serum of senile mice. It is indicated that zuoguiyin acts remarkably on improving metabolism of free radical in senile body,strengthening SOD activity and reducing organic injuries caused by MAD accumulation.

Objective:To explore the effect of zuoguiyin and its partial prescriptions on cellular immune function in senile mice.Methods:Healthy male ICR senile mice were divided into 4 groups at random: old contrast group, ZGY group, prepared rhizome of rehmannia(PROR) group and none prepared rhizome of rehmannia(NPROR) group. The senile mice had been drunk zuoguiyin and its partial prescriptions for 75 days, then observing the differences through thymic index, spleen index, activity of T lymphocyte and the level of IL-2 after using different drugs.Results:Acted by ZGY, ZGY had enhanced cellular immune function of senile mice, appearing that thymic index, spleen index, activity of T lymphocyte and the level of IL-2 were distinctly higher than those of the old contrast group. Compared to ZGY,prepared rhizome of rehmannia also enhanced the activity of T lymphocyte. However, it had no effect on thymic index, spleen index and the level of IL-2.So its effect on anti-senility was not better than ZGY. None prepared rhizome of rehmannia group had no distinct effect on those indexes.Conclusion:Zuoguiyin had an action of antisenility by improving cellular immune function.

Objective To observe the efficacy of the combination of moxibustion and pressing beans on auricular in the treatment of elderly patients with chronic diarrhea.Methods 45 elderly patients met the diagnostic criteria for diarrhea were divided into two groups,the treatment group (23 cases) received the treatment of combined moxibustion with ear pressure beans,the control group (22 cases) received traditional Chinese medicine treatment.The efficacy was observed and compared after two weeks.Results The effective rate reached 95.6％ in the treatment group,81.8％ in the control group (statistically significant difference,odds ratio 1.16).Conclusion Combined ear pressure beans with moxibustion treatment will receive a significant effect for the elderly patients with chronic diarrhea.The therapy is worth clinical application.

ConclusionBushen-Yitalpowder adjuvant to progesterone therapy can improve the clinical outcome in patients with early threatened abortion.

Objective To investigate the value of modified NIHSS and MRI-PC-ASPECTS for the long-term prognosis in posterior circulation ischemic stroke. Methods Seventy eight patients with posterior circulation ischemic stroke were divided into two groups depended on MRS: good outcome group and bad outcome group. General information of patients was collected and vascular risk factors, NIHSS, MRI-PC-ASPECTS of the two groups were compared. Results The scores of admission NIHSS and highest NIHSS of bad outcome group were higher than that of good outcome group. The scores of MRI-PC-ASPECTS, T admission NIHSS (42-admission NIHSS) and T highest NIHSS of bad outcome group were lower than that of good outcome group (P<0.05). The areas under the ROC curves of MRI-PC-ASPECTS+T highest NIHSS, MRI-PC-ASPECTS, T highest NIHSS and T admission NIHSS were 0.907, 0.821, 0.870, 0.744, respectively (P<0.05). Conclusion NIHSS and MRI-PC-ASPECTS can predict the functional prognosis in patients with posterior circulation ischemic stroke.

Prognosis and treatment of primary Sj?gren′s syndrome with renal tubular acidosis

Objective To establish content determination of hydroxyl safflower yellow A in Biyangqing.Methods Hhgh-performance liquid chromatography(HPLC)was used in the determination.A C18 column was used for the separation flow rate Was set at 1.0mL/min,the temperature of the column was set at 30℃,and wavelength of diction was set at 403 nm.with 100.08%average recovery and 0.98%RSD.Conclusion This detrmination method is specific and reproducible and can be used to control the quality of Biyangqing.

Objective　To investigate the relation between ovarian blood-flow resistance index (RI) measured with color Doppler ultrasound and the dosage of pituitary desensitization medication in controlled ovarian hyperstimulation cycle.Methods　Thirty infertile females were randomized into group at routine dose of gonadotropin releasing hormone agonist (GnRHa) (group A) and group at reduced dose of GnRHa (group B).Ovarian arterial blood-flow RI was checked on cycle day 8 and on the day of human chorionic gonadotropin ( HCG) administration with transvaginal color Doppler ultrasound.Results　The ovarian arterial blood-flow RI on cycle day 8,the total dosage and the duration of human manopausal gonadotropin (HMG) administration were significantly reduced in group B. In both groups,the ovarian arterial blood-flow RI was significantly decreased in the ovary with more dominant follicles (follicle diameter >10 mm) compared with the opposite one.Conclusions　In controlled ovarian hyperstimulation cycle,compared with routine protocol of GnRHa administration,reduced dose of GnRHa could decrease the ovarian arterial blood-flow RI,the total dosage and the duration of HMG administration without reducing pregnancy rate.

Objective] Discussion on academic ideas and clinical experience of professor Dong in treatment of varicocele sterility.[Methods]From academic theory, etiology and pathogenesis, therapeutic principle treatment aspects in professor Dong diagnosis and treatment of varicocele sterility certificate academic view and clinical experience, to summarize its characteristic diagnostic medicine.[Results] Professor Dong considers varicocele infertility disease in liver and kidney, etiology and pathogenesis is kidney, liver, blood stasis, based on the kidney essence deficiency, diseases with ease, and blood stasis resistance as the standard, the causal, with kidney and liver qi activating blood method in the treatment of varicocele thought and method has achieved good clinical therapeutic effect. [Conclusion]The clinical experience of professor Dong in treatment of varicocele syndrome is effective, has the value of popularization and application.

Objective To analysis the effect of metformin on the expression of heat shock protein and VEGF in 5637 cell line of bladder cancer. Methods 5637 cell line of bladder cancer were selected, divided into blank control group, metformin 2 mM group, 5 mM group, 10 mM group and 20 mM group, 5637 cell line of bladder cancer were treated in each group.The cell proliferation inhibition rate was detected by MTT colorimetric assay, cell clone formation rate was detected by plate clone formation test, flow cytometry was used to detect cell cycle change, the expression levels of HSP-70, HSP-90α, VEGF were detected by Western blot method.Results Compared with control group, the inhibition rate of 5637 cell line of bladder cancer after 24, 48, 72 h were higher in all concentrations of metformin group(P<0.05), the higher the concentration and the longer the action time, the stronger inhibitory effect on cell proliferation.Compared with control group, the clone formation rate of 5637 cell line of bladder cancer in all concentrations of metformin group was lower, the difference was statistically significant(P<0.05).Compared with control group, the proportion of G1 phase cells were higher, the proportion of S phase cells were lower in in all concentrations of metformin group (P<0.05), the proportion of G2 phase cells in metformin 2 mM group, 5 mM group were lower(P<0.05).Compared with control group, the expression levels of HSP-70, HSP-90α, VEGF protein were lower in all concentrations of metformin group, the difference was statistically significant(P <0.05).Conclusion Metformin has obvious inhibitory effects on the expression of HSP-70, HSP-90α, VEGF in 5637 cell line of bladder cancer, can inhibit cell proliferation and cloning, promote the occurrence of G1 block, cause cell apoptosis, the effect was dose dependent.