1.Isolation of adult cardiac stem cells from rat heart.
Cong LIU ; Dong KUANG ; Guo-ping WANG
Chinese Journal of Pathology 2010;39(8):555-556
Animals
;
Cell Separation
;
methods
;
Female
;
Male
;
Myocytes, Cardiac
;
cytology
;
metabolism
;
Proto-Oncogene Proteins c-kit
;
metabolism
;
Rats
;
Rats, Sprague-Dawley
;
Stem Cells
;
cytology
;
metabolism
2.The correlation study between the changes of intestinal mucosa predominant bacteria and Toll-like receptor 2, Toll-like receptor 4 gene expressions in diarrhea-predominant irritable bowel syndrome patients
Wentao GUO ; Ping LIU ; Lina DONG ; Junping WANG
Chinese Journal of Internal Medicine 2016;55(7):541-543
Based on high throughput sequencing and PCR detection technology,this study has found out that intestinal microbial diversity was impaired and the quantities of two main bacteria flora (Bacteroidetes and Clostridium) were significantly reduced in patients with diarrhea-predominant irritable bowel syndrome (D-IBS).Meanwhile mucosal expression of toll-like receptor (TLR) 2 and TLR4 were significantly enhanced,which was inversely correlated with the reduction of Bacteroidetes and Clostridium.Thus,it suggests that D-IBS may be associated with TLR signal transduction triggered by the intestinal dysbacteriosis.
3.Effect of oral mucosa transplantation in the treatment of severe contracted conjunctival sac after ocular prosthesis implantation
Li-Li, DONG ; Guo-Ping, CAO ; Xue-Qun, YU
International Eye Science 2014;(12):2268-2270
AlM: To observe the clinic effect of oral mucosa transplantation in the treatment of severe contracted conjunctival sac after ocular prosthesis implantation .
METHODS: Thirty-three cases ( 33 eyes ) with globe disorders and severe contracted conjunctval sac were operated ocular prosthesis implantation firstly, and conjunctival sac plasty using oral mucosa after 6mo.
RESULTS: Thirty - one cases were successful, no complications appeared. One case had primary ptosis and 1 case had recurrent conjunctival sac contracture.
CONCLUSlON:lt is recognised that the methods of oral mucosa transplantation in severe contracted conjunctival sac after ocular prosthesis implantation are effective on those cases.
4.IMMUNOFLUORESCENCE ANALYSIS OF MANCHETTE DURING MOUSE SPERMIOGENESIS
Rui GUO ; Ping YAN ; Xixia LI ; Dong ZHANG
Acta Anatomica Sinica 2002;0(06):-
Objective To investigate the localization and the morphological changes of manchette during mouse spermiogenesis.Methods Immunofluorescence staining with FITC and costaining with DAPI were used to demonstrate the cellular localization of the manchette at different stages during mouse spermiogenesis.The structural changes of the manchette were observed during the maturing of the spermatid.Results Immunofluorescence staining showed that manchette existed exactly around the nuclei of the spermatids.Manchette began to form,when the shape of the nucleus changed from spherical to slightly elongated.While the nucleus of the spermatids condensed and elongated at later stages,manchette moved gradually to the caudal position of the spermatids.At last,the manchette diminished as the spermatids became mature.During mouse spermiogenesis,manchette underwent a transition from a cap-like to a tubular configuration.ConclusionThe formation and diminishment of the manchette is in step with the condensation and elongation of the nucleus of the spermatid.Both the structural and positional changes of the manchette coincide with the changes of the nucleus.These results imply that manchette might play an important role in mouse spermiogenesis.
6.Thirty cases of knee osteoarthritis treated by acupuncture and warming needle moxibustion.
Guo-ping XIONG ; Qin HUANG ; Yuan-feng DONG
Chinese Acupuncture & Moxibustion 2011;31(6):551-552
Acupuncture Therapy
;
Adult
;
Aged
;
Female
;
Humans
;
Male
;
Middle Aged
;
Moxibustion
;
Osteoarthritis, Knee
;
therapy
7.Pathways for Nitrobenzene Biodegradation
Li-Wei FAN ; Dong-Lin GUO ; Hui-Juan LIU ; Chang-Hong GUO ; Xiao-Ping WANG ;
Microbiology 1992;0(05):-
Nitrobenzene is one of the toxic compounds. Much work had focused on biodegradation of it sofar. Two main pathways for nitrobenzene biodegradation, oxidative and partial reductive pathways, were reviewed in this article. The mechanism of these pathways including involved enzymes and genes was introduce in details. Comparative analysis of the pathways would provide basis for the development and application of biodegradation technology for nitrobenzene and other organic pollutants.
8.Analysis the characteristic of early myocardial ischemia with functional tomoscintigraphy
Xiaodong LI ; Yu SONG ; Honggang ZHAO ; Zuncheng ZHANG ; Hua DONG ; Ping DONG ; Yongtao GUO ; Yue ZHANG
Journal of Chinese Physician 2008;10(6):724-726
Objective To observe the characteristic changes in cardiac function and to raise diagnostic accuracy and sensibility.Methods 39 silent myocardial ischemia and 58 angina and 46 age-matched healthy subjects and 62 obsolete myocardial infarction underwent myocardial tomography imaging and gated ventricular imaging.Covariance analysis was used to compare difference between SPECT imaging and parameters of function.The age was tested as covariate factor,then SNK-Q test and multiple comparison were carried on.Results The silent myocardial ischemia and angina showed reversible radioactive defect or fixed defect.Most of myocardial SMI showed single peak exempt TPFR.EF,PFR and PER showed remarkably statistical difference (P<0.01).The multiple comparison of PFR and PER was increased according by OMI,AN,SMI and negative control group (P<0.05).Conclusions The myocardial damage and PFR,PER in SMI is lower than that in AN group,which have no remarkably influence on the parameters of diastolic and retractile function in left ventricular.
9.Effect of oligosaccharide esters and polygalaxanthone Ill from Polygala tenuifolia willd towards cytochrome P450.
Zhao-liang LI ; Xian-zhe DONG ; Dong-xiao WANG ; Rui-hua DONG ; Ting-ting GUO ; Yan SUN ; Ping LIU
China Journal of Chinese Materia Medica 2014;39(22):4459-4463
Five compounds (tenuifoliside C, tenuifoliside D, telephiose A, telephiose C and polygalaxanthone III) from polygala tenuifolia wild were incubated together with CYP probe substrate in human liver microsomes to investigate the inhibitory effect towards CYP450 enzyme. Phenacetin (CYP1A2), coumarin (CYP2A6), paclitaxel (CYP2C8), diclofenac (CYP2C9), S-mepheriytoin (CYP2C19), dextromethorphan (CYP2D6), chlorzoxazone (CYP2E1), midazolam (CYP3A) were selected as the isoforfn specific substrate. And the formation of paracetamol, 7-hydroxycoumarin, 6alpha-hydroxy paclitaxel, 4'-hydroxydiclofenac, dextrorphan, 6-hydroxychlorzoxazone, 1'-hydroxymidazolam, 4'-hydroxymephenytoin were detected respectively to measure the effect towards CYP450 by high-pressure liquid chromatography (HPLC). The result shows that five compounds from polygala tenuifolia willd significantly inhibit chlorzoxazone 6-hydroxylation catalyzed by CYP2E1, while showed no effect towards CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP3A. And IC50 value was 38.73, 54.14, 61.77, 62.22, 50.56 micromol x L(-1), respectively.
Cytochrome P-450 Enzyme System
;
metabolism
;
Esters
;
pharmacology
;
Glycosides
;
pharmacology
;
Humans
;
Microsomes, Liver
;
drug effects
;
enzymology
;
Oligosaccharides
;
pharmacology
;
Polygala
;
chemistry
;
Xanthones
;
pharmacology
10.Research on climatic factors of ecology suitability regionalization of atractylodis.
Zhe-tian TAN ; Hao WANG ; Shou-dong ZHU ; Yu-ping YAN ; Lan-ping GUO ; Yu-guang ZHENG
China Journal of Chinese Materia Medica 2015;40(21):4171-4176
Through study on the correlation between atractylodis lactones ingredient content and climatic factors, we research regionalization from climatic of five main producing provinces of the country, in order to provide a scientific basis for atractylodis' conscious cultivation. By sampling from 40 origins which from five main producing provinces of the country, we use SPSS to analysis variation of atractylodis lactones ingredient content in different conditions of climatic factors and the effect of each factors. Then according to the relationship between atractylodis lactones ingredient content and climatic factors, we use ArcGIS to conduct ecological suitability regionalization based on climatic factors. The most suitable climatic condition for cultivation of atractylodis: the wettest month precipitation 220-230 mm, the warmest average temperature 25 degrees C, the average temperature of driest season 10 degrees C.
Atractylodes
;
chemistry
;
growth & development
;
China
;
Climate
;
Ecology
;
Ecosystem
;
Seasons
;
Temperature