Objective To evaluate the estrogen activity of DDE by using MCF-7 cell proliferation bioassay.Methods The estrogen activity of DDE was tested by using the MCF-7 cell multiplication experiment in vitro and the mechanism was explored preliminarily through growth curve analysis.Results DDE showed an obvious estrogenic activity in the MCF-7 cell proliferation and the cell cycle analysis indicated that cell proliferation index in DDE group was significantly higher than that in the control group.Conclusion DDE has the estrogenic activity and the mechanism may be the same as that of E2,that is,binding with the estrogen receptor is the first step of the following biological effects.

Humans ; Organ Transplantation ; legislation & jurisprudence ; Patient Selection ; Tissue Banks ; legislation & jurisprudence ; organization & administration ; Transplant Recipients

AIM: To observe the expression of COX-2 in rat corneal neovascularization (CNV) and its relationship to CNV, and to explore the inhibition of Celecoxib, a COX-2 inhibitor, to CNV.METHODS: The distribution and quantification of COX-2and VEGF was detected by immunohistochemistry. Expression of COX-2 and VEGF mRNA was quantified by RT-PCR.The difference in protein and mRNA expressions of COX-2and VEGF was analyzed to find the correlation between them.RESULTS: Expression of activated COX-2 and VEGF protein and mRNA in CNV had a dynamic change. VEGF and COX-2co-localized. Compared with the control group, expression of both protein, mRNA of COX-2 and VEGF in experimental group Ⅱ and Ⅲ had significant difference (P＜0.05), indicating the correlation between COX-2 and VEGF, while that in experimental group I had no statistical difference (P＞0.05).CONCLUSION: COX-2 expression was up-regulated in inflammatory CNV. COX-2 modulates the expression of VEGF,playing a very important role in CNV. Celecoxib inhibit COX-2expression so as to hold back the CNV.

Objective To study the expressions of cyclooxygenase-2(COX-2) and epidermal growth factor receptor(EGFR) in tumor tissues and explore the effect of COX-2 and EGFR in NSCLC development.Methods The expression of COX-2 and EGFR was detected by immunohistochemical technique in 56 cases of non-small cell lung cancer(NSCLC) and in 20 cases of normal lung tissues.Results The expression of COX-2 and EGFR in NSCLC was significantly higher than that in normal lung tissues(P

Emotion recognition will be prosperious in multifarious applications, like distance education, healthcare, and human-computer interactions, etc. Emotions can be recognized from the behavior signals such as speech, facial expressions, gestures or the physiological signals such as electroencephalogram and electrocardiogram. Contrast to other methods, the physiological signals based emotion recognition can achieve more objective and effective results because it is almost impossible to be disguised. This paper introduces recent advancements in emotion research using physiological signals, specified to its emotion model, elicitation stimuli, feature extraction and classification methods. Finally the paper also discusses some research challenges and future developments.
Electrocardiography ; Electroencephalography ; Emotions ; physiology ; Facial Expression ; Gestures ; Humans ; Models, Theoretical

Objective To explore achillis tendon-sliding procedure and subtalar joint capsula release by the same cut and individualization treatment used in the correction of congenital clubfoot.Methods Forty eight cases (76 feet) of congenital clubfoot who were first visit were treated by achillis tendon-sliding procedure and subtalar joint capsula release by the same cut,meantime,footplate fascia release and transfer of the anterior tibial tenden were made by individualization.Results All cases were followed-up,the average time follow-up was 1 year and 6 months,excellent were 71% and good were 15.7%.Five cases were recurrence and its rate was 6.94%.Conclusions Achillis tendon-sliding procedure and subtalar joint capsula release by the same cut can solve rotation of displacement between calcaneus and talus and individualization treatment can also solve high arch deformity and adduction of foot.This technique is a simple,safe and effective treatment for congenital clubfoot.

Objective Due to the incompleteness and complexity of fault diagnosis for power transformers, a comprehensive rough-fuzzy scheme for solving fault diagnosis problems is presented. Fuzzy set theory is used both for representation of incipient faults' indications and producing a fuzzy granulation of the feature space. Rough set theory is used to obtain dependency rules that model indicative regions in the granulated feature space. The fuzzy membership functions corresponding to the indicative regions, modelled by rules, are stored as cases. Results Diagnostic conclusions are made using a similarity measure based on these membership functions. Each case involves only a reduced number of relevant features making this scheme suitable for fault diagnosis. Conclusion Superiority of this method in terms of classification accuracy and case generation is demonstrated.

ObjectiveTo investigate the possible mechanism of common bile duct ligation (CBDL) preconditioning alleviating subsequent renal ischemia/reperfusion injury.MethodsC57BL/6 mice was divided into 3 groups.In CBDL group,the common bile duct was transiently ligated at day 0 (d0),and recanalized after two days (d2) and two days later (d4). Unilateral renal ischemia/reperfusion injury was performed.Sham operation was done in Sham group at d0,then unilateral renal I/R was performed at d4. Control group was set as normal contrast. Total bilirubin (TB) was monitored.24 h after I/R (d5),we collected blood to detect creatinine (Cr),and the expression of heat-shock protein 27 (HSP-27) and endothelial nitric oxide synthase (eNOS) was monitored by Western blotting at d0,d2,d4,d5 four time points.ResultsAfter CBDL,TB fell to normal at d4.Cr levels in CBDL group were lower than in Sham group (P＜0.05),the protein expression levels of HSP-27 and eNOS were increased from d2 and sustained to d5 in CBDL group as compared with Sham group ( P ＜ 0.05 ).Conclusion CBDL preconditioning can obviously alleviate renal ischemia/reoerfusion injury in mice probably by up-regulating the expression of HSP-27 and eNOS.

BACKGROUND: Neurotrophin-3 (NT3) and neurotrophin-4 (NT4) play an important role in the growth, survival and functions of neurons in vivo or in vitro. However, it has not been reported whether they can protect and repair neurons of spinal cord after crushed spinal cord injury (cSCI).OBJECTIVE: To explore the expression of NT3 and NT4 in ventral and dorsal horns of spinal cord at various time points after cSCI and analyze the pattern of expression changes.DESIGN: Randomized controlled study and single analysis of variance.SETTING: Continuing Education College of Beijing Union University;Department of Anatomy, Kunming Medical College.MATERIALS: The experiment was conducted in the Institute of Neuroscience of Kunming Medical College from January to March 2003.Totally 24 adult SD rats were divided randomly into 4 groups: control group, 24-hour cSCI group, 7-day cSCI group, and 21-day cSCI group with 6 rats in each group.METHODS: After anesthesia, rats in each cSCI group were dissected at vertebra T11 and crushed at spinal cord T13. After 24 hours, 7 days and 21days, the rats were killed respectively by cutting their heads off, and spinal cord L1-L3 was taken out to make frozen transactional slices of 20 μm.Rats in control group received no treatment and were killed at the same time point as those in 24-hour cSCI group, and the process of slice making was the same as mentioned above. The distribution of NT3 and NT4 positive cells in the spinal cord ventral and dorsal horns of adult rats was observed, and NT3 and NT4 positive nucleus in the ventral and dorsal horns of the same areas were counted.neurons in the rats' spinal cord ventral and dorsal horns.products were mainly stained in nucleus while NT4 was stained in horns of spinal cord in each group: The number of positive neurons in the ventral horn was significantly greater in 7-day and 21-day cSCI groups than in control and 24-hour cSCI groups (10.2±1.1, 11.4±3.2, 6.2±1.8, 7.4±2.4, P＜0.01). The number of positive neurons in the dorsal horn in 21day group was significantly greater than that in control group (86.4±9.8,71.3±8.3, P＜0.01); however, it was remarkably lower in 24-hour and 7day cSCI groups than in control group (48.5± 5.1, 41.5±3.7, 71.3± 8, P cord in each group: The number of positive neurons in the ventral horn was significantly higher in 24-hour, 7-day and 21-day cSCI groups than in control group (9.4±2.8, 10.8±2.7, 15.1±4.0, P＜0.05); and it assumed an increasing tendency as time went by (P＜0.05). By contrast, the number of positive neurons in the dorsal horn in 7-day and 21-day groups was significantly higher than in control and 24-hour groups (28.1±3.1,35.1±4.4, 23.3±2.3, 24.1±1.8, P＜0.01), and it would increase with time following cSCI (P＜0.01).CONCLUSION: The number of NT3 and NT4 positive neurons increases in spinal cord ventral and dorsal horns after cSCI, suggesting that endogenous NT3 and NT4 have effect at different time on sensory and motor neurons in the repair of spinal cord injury.

To observe the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, in inhibiting the growth of transplanted A549 tumors in nude mice and the expressions of p-Akt and HIF-1alpha in A549 tumors.