For the patients with early breast cancer,the effects of breast-conserving surgery combined with radiotherapy and radical resection are equal,and the former shows less adverse reactions and better aes-thetic outcome. Because of individual differences and the inherent complexity of tumor,to obtain optimal effects,it is an inevitable trend of making an individual comprehensive therapy,which is a combination of radiotherapy,chemotherapy,endocrine therapy and targeted therapy.

Objective To explore the effects of prenatal exposure to lead on learning and memory of rats' offspring. Methods The pregnant rats were randomly divided into 4 groups provided with double evaporated water, 50, 100 and 200 mg/L lead acetate solution via drinking water respectively. The lead-exposure period for exposure groups was limited from the 1st day after pregnancy to the 20th day when the offspring began to be weaned. For learning ability 20-day old offspring were tested by water maze. For active learning and memory ability, the 20-day old, 40-day old and 60-day old offspring were tested by Y maze. Results The frequency of the mistakes in water maze made by offspring increased with the increase of the prenatal lead-exposure doses of their mothers and showed significantly higher levels in 100 and 200 mg/L groups compared with that of control group (P0.05). But the qualified rates of 0-min and 24-h escape for 100 and 200 mg/L groups showed sigificantly lower levels compared with that of control group when they were 60-day old (P

Female ; Humans ; Infant ; Metabolism, Inborn Errors ; Methylmalonic Acid ; blood

Objective To investigate the feasibility and clinical effects of the pair ringer technology for bony avulsion of the posterior cruciate ligament under arthroscopy. Methods From January 2005 to July 2009, 23 patients were treated with the pair ringer technology for bony avulsion of the posterior cruciate ligament by arthroscopy. There were 15 male and 8 female, with an average of 39.3 years (ranging from 28 to 52 years). There were 4 cases of type Ⅱ, 14 type Ⅲ, and 5 type Ⅳ according to Meyer-Mckeever classification. The outcome measures were X-ray and MRI testing, Lysholm and Tegner knee function score. Results All the operations were finished in an hour. There were no complications as nerve injury or infection occurred in the study. The patients were followed-up for an average of 16.5 months (12-23 months). The Lysholm score rose from 50.3±6.1 preoperatively to 89.7±8.3 at the six months postoperatively (t=18.34, P=0.0007). The Tegner score rose from 1.7±0.5 preoperatively to 5.7±1.3 at the six months postoperatively (t=13.77,P=0.0008). All of the patients recover a functionally stable knee and have considerably improved knee function compared with their preoperative status. Conclusion The pair ringer technology under arthroscopy is a liable method for bony avulsion of the posterior cruciate ligament. This minimally invasive technology leads to rigid fixation, early rehabilitation.

The electrochemical behaviors of parathion(PT) on nano-Al2O3 film modified electrode(nano-Al2O3/GCE/CME) were investigated. It was found that the redox peak currents of PT remarkably increased and the oxidation peak potential negatively shifted at the nano-Al2O3/GCE/CME. All the experimental parameters were optimized and a simple and sensitive electroanalytical method was developed for the determination of PT. The oxidation peak current was linear with the concentration of PT over the range from 2.5×10-9 to 1.0×10-7 mol/L and 1.0×10-7 to 1.0×10-5 mol/L. A low detection limit of 1.0×10-9 mol/L was obtained for 30 s accumulation at open circuit(S/N=3). The relative standard deviation(n=10) was 3.8% for 1×10-5 mol/L PT. The proposed method was used for the quantitative analysis of PT in some vegetables and the results were satisfactory.

This report presented a case of 75-year-old woman who had received drug treatments two months earlier for nephrotic syndrome and was admitted to our hospital for inferior wall myocardial infarction with elevated creatinine and anemia.Kidney pathology after myocardial infarction showed allergic acute interstitial nephritis which induced acute renal failure.We stopped tripterygium glycosides and used cortical hormone,consequently.Thereafter,the symptoms of renal failure and anemia were improved and we considered tripterygium glycosides resulted in above allergic acute interstitial nephritis and anemia.Therefore,we had to carry out renal needle biopsy in the patient with the elderly nephrotic syndrome before confirmatory treatment to avoid blind use of tripterygium glycosides.

Objective To provide the experimental basis for the further research of the interacting proteins with Stathmin ,the Stathmin gene Pichia pastoris expression system was constructed ,the expressed Stathmin product was purified and identified .Meth‐ods Stathmin gene was amplified from tumor cell line of SKBR3 by PCR method and cloned into the yeast expression vector pPIC3 .5K .The recombinant vector pPIC3 .5K‐Stathmin was constructed and transformed into Pichia pastoris GS115 .The positive clones were screened by YPD medium containing Geneticin 600 μg/mL .Expression was induced with 0 .5% methanol and expres‐sion products were identified by SDS‐PAGE and Western Blotting .Results DNA sequencing result showed that the gene fragment was consistent with Stathmin gene sequence .pPIC3 .5K‐Stathmin was selected from YPD culture medium containing Geneticin ,and the positive clones were identified by PCR .SDS‐PAGE showed that a 37 × 103 protein band could be seen on the PAGE gel after Coomassie Blue staining ,which was further confirmed and identified as Stathmin protein by Western Blotting .Conclusion Stathmin yeast expression vector is successfully constructed and expressed in Pichia pastoris ,which laid the foundation for the study of inter‐acting proteins with Stathmin ,and for the preparation of the biological treatment drugs of Stahtmin target .

Objective To investigate the effect of percutaneous vertebroplasty and kyphoplasty (PKP) on bone density and serum leptin in patients with osteoporotic thoracolumbar compression fractures. Methods One hundred and two patients with osteoporotic thoracolumbar vertebral compression fractures and 100 cases of healthy control were treated with PKP surgery. The serum leptin and adiponectin, and the bone mineral density of the lumbar spine were determined. Results patients Levels of the serum leptin and adiponectin in patients with osteoporotic thoracolumbar vertebral compression fracture were significantly higher than those with non osteoporotic fracture patients (P < 0.05). One month post-PVP, the levels of serum leptin and adiponectin in the two groups were significantly decreased (P < 0.05). postoperative group of The lumbar bone density at 1 month post-operation was is significantly higher than that before operation in each group (P < 0.05, respectively), with no significant difference between the two groups. The levels of serum leptin and adiponectin were negatively correlated with the bone mineral density (BMD) (P < 0.05). Conclusions tLevels of serum adiponectin and leptin were both increased in osteoporotic or non bone osteoporosis thoracolumbar vertebral compressor fractures. The percutaneous vertebroplasty forming operation could effectively reduce the serum level of leptin and adiponectin, and improve the bone mineral density of the lumbar vertebrae.

Objective To evaluate the potential effects of RS504393, CC chemokine receptor (CCR) 2b and CCR1 antagonist, on LPS-induced acute lung injury (ALI) and to investigate the underlying mechanisms. Method A549 cell line was stimulated with LPS (10 μg/mL) and then treated with RS504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor (PAI)-l,monocyte chemoattractant protein (MCP)-2,and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry. Results In A549 cell line treated with RS504393,the expressions of CCR1, CCR2b and IL-8 were significantly inhibited after LPS stimulation. In rats with LPS-induced ALI, treatment with RS504393 significantly protected mice against lung injury by attenuating influx of leukocytes and protein into bronchoalveolar space and by lessening pathological changes of lung. Treatment with RS504393 down-regulated IL-1β and PAI-1 expressions in bronchoal veolar lavage fluid (BALF) and lungs at mRNA and protein levels along with up-regulation MCP-2 expression compared to rats of vehicle-treated groups. Conclusions CCR2b and CCR1 play pivotal roles in the development of ALl,and RS504393 as a antagonist can halt the development of ALI.

Combined Modality Therapy ; High-Frequency Ventilation ; methods ; Humans ; Infant, Newborn ; Nitric Oxide ; administration & dosage ; Persistent Fetal Circulation Syndrome ; drug therapy ; Pulmonary Surfactants ; administration & dosage