1.Impact of the peripheral neurotomy method combined with rehabilitation exercise on gross motor function and balance function of children with cerebral palsy
Junying YUAN ; Hui DONG ; Xianxu ZENG ; Erliang SUN ; Jun WANG
Chinese Journal of Applied Clinical Pediatrics 2014;29(20):1578-1582
Objective To study the impact of the peripheral neurotomy method combined with rehabilitation exercise on gross motor function and balance function of children with cerebral palsy.Methods Matched pair design was used in the study.Thirty children with spastic cerebral palsy hospitalized from May 2011 to Nov.2013 suffering from peripheral neurotomy were assigned as study group,who were conscious and treated by peripheral neurotomy com bined with rehabilitation exercise.In the meanwhile,30 children inpatients at the same time were employed as the control group,who were similar in age and symptoms,with the same gender and the same Gross motor function classification (GMFCS) grade,and the controls were treated only with ordinary rehabilitation exercise.Modified Ashworth score,Gross Motor Function Measure (GMFM) D and E regions scores,physicians rating scale score in the 2 groups were recorded after treatment for 2,4,6,8,10 weeks.Repeated measure analysis of variance method was introduced to analyze the differences between the 2 groups in improved Ashworth muscle tension,gait improvement,gross motor function and balance function.Results The study group was superior over the control group in muscle tension reduction (F =8.177,P =0.006) and gait improvement(F =24.284,P =0.000).The 2 groups were not different statistically in D region evaluation of GMEM (F =0.072,P =0.790) and E region evaluation of GMFM (F =0.000,P =0.985) ; For Berg balance scale,the 2 groups had also no difference(F =0.150,P =0.700).Conclusions Both peripheral neurotomy method combined with rehabilitation exercise and the ordinary rehabilitation exercise method both could improve the gross function and the balance function.Furthermore,the former was superior over the latter in muscle tension reduction and gait improvement.
2.Deviation factors of posterior atlantoaxial transarticular screw placement assisted by rapid prototyping drill templates
Yong HU ; Zhenshan YUAN ; Hui XIE ; Jianbing YUAN ; Weixin DONG ; Chengtao WANG
Chinese Journal of Trauma 2013;29(10):946-954
Objective To validate the safety and accuracy of a rapid prototyping drill template (RPDT) for posterior atlantoaxial transarticular screw placement and analyze factors for screw deviation.Methods Twelve normal cadaveric cervical spines were examined using 64 slice CT with 1-mm thick scan and data in Dicom format were recorded.After data was processed using software Mimics 10.01 for three-dimensional (3-D) model reconstruction,computer-assisted design of optimum trajactory for atlantoaxial transarticular screw placement was worked out and made into a drill template,where the surface was created as the inverse of axial posterior surface.The drill template was materialized in a rapid prototyping machine and used to place the screws.After surgery,the position of posterior atlantoaxial transarticular screw was evaluated by X-ray and CT scan.Screw entry point,angle and orientation of the optimal and actual trajactory were determined after fitting the position of the pre-operative and post-operative specimen in computer software and the redefining the 3-D coordinate axis.Results Twenty-four screws were implanted with no cortex perforation.Depth of the optimum save screw trajectory for atlantoaxial transarticular fixation was (37.34 ± 2.31) mm on the left side and (37.11 ± 2.21) mm on the right side.Introversion angles of the optimum save screw trajectory was 0° in both sides,but the actual angle was (0.15 ±0.58)°on the left side and (0.11 ±0.46)°on the right side.Elevation angle of the optimum save screw trajectory was (49.35 ± 1.62) °on the left side and (48.83 ± 1.83) ° on the right side,but the actual angle was (49.29 ± 1.68) °and (49.10 ± 1.45) °respectively.Average displacement of screw entry point in the x,y and z axis was respective (0.21 ±0.65) mm,(0.69 ± 1.48) mm and (0.39 ±0.11) mm on the right side,while (0.19 ± 0.66) mm,(0.53 ± 1.45) mm and (0.38 ± 0.13) mm on the left side.There were no statistically significant differences in deviation levels of entry point and orientation between the optimum and actual screw trajectory (P > 0.05).Conclusions Causes for deviation in RPDT-assisted placement of atlantoaxial transarticular screw are mainly intrinsic factors of the hardware and software and human factors in the operation.RPDT is easy in operation and has individualized design,which greatly improves the accuracy of screw placement and reduces screw deviation.RPDT can be widely used in clinical practice.
3.Sensitivity of Human Glioblastoma Multiforme Cell Line BT325 to Antineoplastic Drugs
Ming HAN ; Fang YUAN ; Liping DONG ; Zhongfang SHI ; Hui YUAN ; Shaohua YANG
Chinese Journal of Rehabilitation Theory and Practice 2011;17(9):840-843
Objective To examine the sensitivity of human glioblastoma multiforme cell line BT325 to 5 antineoplastic drugs, including cisplatin (DDP), teniposide (VM26), nimustine (ACNU), temozolomide (TMZ) and vincristine (VCR). Methods BT325 cells were incubated in DMEM with 10% or 20% fetal bovine serum (FBS) or without FBS respectively. The cell numbers were counted at 24 h, 48 h, 72 h,96 h, 120 h, and 144 h, then platting and growth curve were drafted. Cell counting kite-8 was used to detect the influence of 5 drugs with different concentrations on human glioma cell line BT325. Results DDP and VM26 significantly suppressed BT325 cells(>75%) viability in a dose-dependent manner, while VCR inhibited BT325 cells (50%) growth without dose-effect relationship. In contrast, ACNU and TMZwere not effective on the viability of BT325 cells. Conclusion BT325 cells were very sensitive to chemotherapeutic drugs DDP amd VM26.
4.Mediating effect of psychological resilience between perceived organizational support and presenteeism among medical staff in the prevention and control of COVID-19
DONG Wan er CHEN Wang zhong YUAN Xiang dong HE Hui tao
China Occupational Medicine 2022;49(05):542-
Objective ( )
To explore the influence of perceived organizational support POS and psychological resilience on
( - )Methods
presenteeism behavior in medical staff in the prevention and control of coronavirus disease COVID 19 . Doctors
- -
and nurses engaged in the front line work of prevention and control in the four designated hospitals for the treatment of COVID
,
19 in Guangdong Province Hunan Province and Jiangsu Province were selected as the research subjects using a random cluster
, - -
sampling method. The Survey of Perceived Organizational Support Scale the 10 item Connor Davidson Resilience Scale and
- , Results
Standford Presenteeism Scale 6 were used to investigate their POS psychological resilience and presenteeism. The
, ( ),( ) ( ),
total scores of POS psychological resilience and presenteeism were 44.9±7.6 31.2±5.7 and 18.5±3.7 respectively.
(
Both POS and psychological resilience had negative effect on presenteeism standardized regression coefficient were −0.59
, P ) [
and −0.38 both <0.01 . Psychological resilience played a partial mediating role between POS and presenteeism 95%
( - ), ] Conclusion
confidence interval −0.22 −0.09 the mediating effect ratio was 23.4% . Both POS and psychological
- ,
resilience can directly affect the presenteeism behavior of medical staff in COVID 19 prevention and control and POS can also
indirectly affect their presenteeism by affecting psychological resilience.
5.Mediating effect of psychological resilience between perceived organizational support and presenteeism among medical staff in the prevention and control of COVID-19
DONG Wan er CHEN Wang zhong YUAN Xiang dong HE Hui tao
China Occupational Medicine 2022;49(05):542-
Objective ( )
To explore the influence of perceived organizational support POS and psychological resilience on
( - )Methods
presenteeism behavior in medical staff in the prevention and control of coronavirus disease COVID 19 . Doctors
- -
and nurses engaged in the front line work of prevention and control in the four designated hospitals for the treatment of COVID
,
19 in Guangdong Province Hunan Province and Jiangsu Province were selected as the research subjects using a random cluster
, - -
sampling method. The Survey of Perceived Organizational Support Scale the 10 item Connor Davidson Resilience Scale and
- , Results
Standford Presenteeism Scale 6 were used to investigate their POS psychological resilience and presenteeism. The
, ( ),( ) ( ),
total scores of POS psychological resilience and presenteeism were 44.9±7.6 31.2±5.7 and 18.5±3.7 respectively.
(
Both POS and psychological resilience had negative effect on presenteeism standardized regression coefficient were −0.59
, P ) [
and −0.38 both <0.01 . Psychological resilience played a partial mediating role between POS and presenteeism 95%
( - ), ] Conclusion
confidence interval −0.22 −0.09 the mediating effect ratio was 23.4% . Both POS and psychological
- ,
resilience can directly affect the presenteeism behavior of medical staff in COVID 19 prevention and control and POS can also
indirectly affect their presenteeism by affecting psychological resilience.
6.Mediating effect of psychological resilience between perceived organizational support and presenteeism among medical staff in the prevention and control of COVID-19
DONG Wan er CHEN Wang zhong YUAN Xiang dong HE Hui tao
China Occupational Medicine 2022;49(05):542-
Objective ( )
To explore the influence of perceived organizational support POS and psychological resilience on
( - )Methods
presenteeism behavior in medical staff in the prevention and control of coronavirus disease COVID 19 . Doctors
- -
and nurses engaged in the front line work of prevention and control in the four designated hospitals for the treatment of COVID
,
19 in Guangdong Province Hunan Province and Jiangsu Province were selected as the research subjects using a random cluster
, - -
sampling method. The Survey of Perceived Organizational Support Scale the 10 item Connor Davidson Resilience Scale and
- , Results
Standford Presenteeism Scale 6 were used to investigate their POS psychological resilience and presenteeism. The
, ( ),( ) ( ),
total scores of POS psychological resilience and presenteeism were 44.9±7.6 31.2±5.7 and 18.5±3.7 respectively.
(
Both POS and psychological resilience had negative effect on presenteeism standardized regression coefficient were −0.59
, P ) [
and −0.38 both <0.01 . Psychological resilience played a partial mediating role between POS and presenteeism 95%
( - ), ] Conclusion
confidence interval −0.22 −0.09 the mediating effect ratio was 23.4% . Both POS and psychological
- ,
resilience can directly affect the presenteeism behavior of medical staff in COVID 19 prevention and control and POS can also
indirectly affect their presenteeism by affecting psychological resilience.
7.Research on building method of spleen kidney Yang deficiency diarrhea rats model.
Xin PAN ; Chang-Jiang HU ; Yuan-Yuan GENG ; Ling ZHAO ; Wen-Hui WU ; Xiao-Qing WU ; Zhen-Dong ZHONG
China Journal of Chinese Materia Medica 2014;39(23):4658-4663
Spleen kidney Yang deficiency (SKYD) diarrhea is a common syndrome in tranditional Chinese medicine (TCM). Until now, there is not an ideal SKYD diarrhea rat model for the research. In this study, we compared single factor way (method I, injecting hydrocortisone and gavaging Sennae Folium) with compound factors way(method II, gavaging adenine, improper diet, exhaustion, and gavaging Sennae Folium) on establishing SKYD diarrhea rat model. After modelling, diarrhea index, D-xylose excretory rate, NOS/cGMP signal transduction system, organ index and histopathology examination were used to evaluate the two ways. The results showed that, compared with health group, all the assessment criterias of method I and method II had significant differences (P < 0.01, 0.05). In addition, the index such as diarrhea index, NOS/cGMP signal transduction system, organ index (kidney, testis and thymus) and histopathology examination had significant differences (P < 0.01, 0.05) between method I and method II. In conclusion, the compound factors modelling method better conforms to the symptom of diarrhoea model caused by SKYD. This new modelling method provides a basis for studying on TCM astringents warming and tonifying the spleen and kidney, relieving diarrhea.
Animals
;
Diarrhea
;
metabolism
;
pathology
;
physiopathology
;
Disease Models, Animal
;
Humans
;
Kidney
;
pathology
;
physiopathology
;
Male
;
Rats
;
Rats, Sprague-Dawley
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Spleen
;
pathology
;
physiopathology
;
Xylose
;
metabolism
;
Yang Deficiency
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metabolism
;
pathology
;
physiopathology
8.Central Blood Pressure and the Extent of Coronary Artery Disease
Yuan-Yuan DUAN ; Hui-Liang LIU ; Dong-Xing MA ; Sheng-Li YANG ; Jian-Ping LUO ; Ying LIU ; Wei HAN ;
Chinese Journal of Hypertension 2006;0(10):-
50%.The patients were categorized into as:one-,two-, and three-vessels coronary artery disease group.Central aortic SBP and DBP was measured by cathetarization dur- ing angiography of coronary artery and brachial blood pressure was measured using cuff method.Results Periph- eral SBP,PP and ascending aortic SBP,PP,fractional systolic pressure(FSP=SBP/MAP)were increased and as cending aortic fractional diastolic pressure(FDP=DBP/MAP)was reduced when the diseased coronary vessels were increased(P
9.Identification of Scolopendra subspinipes mutilans and its adulterants using DNA barcode.
Hong-Yin ZHANG ; Jun CHEN ; Jing JIA ; Dong LIU ; Lin-Chun SHI ; Hui ZHANG ; Jing-Yuan SONG ; Hui YAO
China Journal of Chinese Materia Medica 2014;39(12):2208-2211
In this study, the COI barcode was used to identify the Scolopendra medicinal materials and its adulterants in order to provide a new method for the identification of Scolopendra. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence alignment and NJ tree construction was carried out by MEGA6.0 software. The results showed that the COI sequences can be obtained from all experimental samples. The average inter-specific K2P distance of Scolopendra was 0.222 and the minimum inter-specific distance was 0.190. All the Scolopendra subspinipes mutilans medicinal samples clustered into a clade in the NJ tree and can be distinguished from its adulterants. In a conclusion, COI can be used to correctly identify Scolopendra medicinal materials, and it will be a potential DNA barcode for identifying other animal medicinal materials.
Animals
;
Arthropod Proteins
;
genetics
;
DNA Barcoding, Taxonomic
;
methods
;
Drug Contamination
;
prevention & control
;
Electron Transport Complex IV
;
genetics
;
Medicine, Chinese Traditional
;
Molecular Sequence Data
;
Phylogeny
;
Quality Control
;
Scorpions
;
classification
;
enzymology
;
genetics
10.Observation of tirofiban's effect on myocardial necrosis biomarker in patients with aspitin resistance following percutaneous coronary interventions
Yueping LI ; Yujie ZHOU ; Yan ZHANG ; Yingxin ZHAO ; Yuyang LIU ; Hui YUAN ; Lei DONG
Clinical Medicine of China 2008;24(5):443-446
Objective To investigate the effect of tirofiban on myocardial necrosis biomarker after percutaneous coronary interventions(PCI)in patients with aspirin resistance(AR).Methods 374 consecutive patients with aspirin 100 mg≥1 week,receiving no other antiplatelet therapy,scheduled for PCI were enrolled.all patients were given an loading dose of 300 mg clopidogrel at least 12 hours before PCI and an 75 mg maintenance dose per day.Patients were randomized into tirofiban group(n=38)and control group(n=45)after PCI.The levels of CKMB,TnI at 8,12,and 24 hours after PCI were measured in all patients;if the CK-MB,TnI value was above normal upper limitation,it was considered elevated.Results 83 patients were AR(22.2%)and 54.2%of them are females.The frequencies of CK-MB elevation were 15(39.5%)in tirofiban group and 19(42.2%)in control group,and TnI elevation was 18(47.4%)and 23(51.1%)in the two groups respectively.Conclusion Tirofiban can not decrease the elevation level of CK-MB and TnI in patients with AR after PCI.