1.Biomarkers for gastric cancer stem cells
Journal of International Oncology 2012;39(1):45-47
Some specific markers are useful of separating and identifying gastric cancer stem cells.They are including gastric stem cells,general tumor stem cells and mesenchymal tissue stem cells.At present,more and more new biomarkers are discovered besides familiar CD133 and CD44.Combined detection helps to separate pure gastric cancer stem cells and promote the therapy of gastric cancer.
2.Purification and Characterization of Lipases from Rhizopus chinensis
Zhen-Hua RUAN ; Dong WANG ; Yan XU ;
Microbiology 1992;0(06):-
Two lipase active fractions Lip1 and Lip2 were purified from the cell extract of Rhizopus chinensis CCTCCM201021.Both gave a single band on SDS-PAGE after using ammonium sulfate precipitation、Phenyl-Sepharose FF、DEAE-Sepharose FF and Sephadex G100 gel filtration chromatographies.The molecular masses of two lipases were 59.2kD and 39.4kD respectively.Lip1 and Lip2 showed optimal pH at 8.0 and 8.5 and their optimal temperatures were 40℃ and 35℃ respectively.The substrate specificity of the two lipases was obviously different.Lip1 was more specific to long chain fatty acid of p-nitrophenyl esters while Lip2 had a preference for the hydrolysis of short chain fatty acid of p-nitrophenyl esters.Lip1 had 1,3-position specificity for triacylglycerols hydrolysis while Lip2 had nonspecific position.Both lipases were stimulated by Ca 2+、Mg 2+ while SDS had strong inhibition on their activities.Lip1 and Lip2 had good stability in cyclohexane、hexane、heptane and isooctane(30% V/V).
4.Effect of lovastatin and probucol on intracellular cholesterol content in foam cells derived from mouse peritoneal macrophages
Dong WANG ; Hua DING ; Hongyan XU ; Xinbing WEI
Chinese Pharmacological Bulletin 1986;0(06):-
AIM To examine the effects of lovastatin, probucol on the intracellular cholesterol content and cholesterol efflux. METHODS With the model of foam cells through depositing the oxidized low-density lipoprotein (OXLDL) into mouse peritoneal macrophages, intracellular cholesterol content was examined by high performance liquid chromatography after the administration of lovastatin and probucol at different concentration. RESULTS The CE/TC ratios of the model group were beyond 50% and extremely higher than those of the normal and negative groups, suggesting that the model of foam cells was established triumphantly. Lovastatin decreased intracellular TC, CE and CE/TC significantly and dose-dependently. Probucol also possessed such effects on intracellular TC, CE, but no dose-dependently, and no effect on the ratio of CE/TC. CONCLUSION The results showed lovastatin might be conducive to plagues stability by increasing cholesterol efflux from the foam cells and decreasing the intracellular cholesterol content. Probucol did not have this effect although it decreased intracellular TC and CE content.
5.Dynamic Expression of HoxB5 in Lung Tissue of Neonatal Rats with Hyperoxia-Induced Chronic Lung Disease and Its Significance
wei, XU ; jian-hua, FU ; xin-dong, XUE
Journal of Applied Clinical Pediatrics 1994;0(04):-
0.05),and the expression of HoxB5 in the model group tapered after the 7th day,but the expression of HoxB5 increased and reached the peak on the 7th day and expressed in a stable level in the control group and were significantly higher than those of model group(Pa
6.A case of hemorrhage of anonym after tracheotomy.
Jian-hua ZHANG ; Shi-xiong TANG ; Xu-dong CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(1):69-70
Anastomosis, Surgical
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adverse effects
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Brachiocephalic Trunk
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pathology
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Hemorrhage
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etiology
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therapy
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Humans
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Larynx
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surgery
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Male
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Middle Aged
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Postoperative Complications
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Trachea
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surgery
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Tracheal Stenosis
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etiology
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surgery
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Tracheotomy
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adverse effects
7.Treatment progress of diffuse large B-cell lymphoma: reports from the 57th American Society of Hematology annual meeting
Tingxun LU ; Dong HUA ; Wei XU ; Jianyong LI
Journal of Leukemia & Lymphoma 2016;25(1):19-22
Diffuse large B-cell lymphoma (DLBCL) is one of the most common non-Hodgkin lymphomaaccounting for 30 %-40 %. The most common first-line therapy for DLBCL is rituximab in combination withchemotherapy. About two thirds of patients treated by the first-line therapy achieve a complete remission (CR)and are cured finally, but nearly one third of patients can not reach CR after frontline treatment appearingrefractory or relapse early, especially for the high risk patients or cases with MYC alterations, the regimenimproving the long-term survival is not much. In the 57th American Society of Hematology (ASH) annualmeeting, a plenty of treatment as focus on these patients brought in encouraging results, which makes itpossible to further improve the CR rate. The progresses on DLBCL of relapse and refractory, high risk andspecial types were summarized in this paper based on the reports in the 57th ASH annual meeting.
8.Investigation and analysis of status of research and research utilization in practice among nurses of hospital of Traditional Chinese Medicine
Hua ZHANG ; Guihua XU ; Zhiling SUN ; Haixia GAO ; Yinfeng DONG
Chinese Journal of Practical Nursing 2014;30(27):42-45
Objective To investigate the status of research and research utilization in practice among nurses of hospital of Traditional Chinese Medicine.Methods Two hundred and sixty nurses of hospital of Traditional Chinese Medicine were selected to fill out the questionnaire.Results The rates of involvement of research activities and research utilization among nurses of hospital of Traditional Chinese Medicine were low.The score of every factor ranged from the higher to the lower were communication,organization,research and nurse.Conclusions Involvements of research activities and research utilization among nurses of hospital of Traditional Chinese Medicine are insufficient.Most factors associated with nurses' low extent of research utilization are objective factors.It is pivotal for nursing managers to construct supportive professional environment,improve nurses' research and information skills and the capacity of the research utilization.
10.Anticoagulant treatment of non-valvular atrial fibrillation in cardiovascular department
Chaohui DONG ; Hua XIAO ; Huanjie ZHENG ; Jiawei XU
The Journal of Practical Medicine 2016;32(16):2737-2739
Objective To investigate the present situations of anticoagulant treatment of non-valvular atri-al fibrillation in order to get better guidance of the clinical anticoagulant therapy and reduce the incidence of stroke caused by atrial fibrillation. Methods The clinical data of NVAF patients hospitalized in the first affili-ated hospital of Chongqing Medical University from January 1 , 2012 to December 31 , 2014 were retrospectively reviewed and analyzed in terms of basic characteristics , medical treatment plan and INR monitoring. Results A total of 1,390 patients were enrolled, 85.97% of whom needed anticoagulant therapy and 25.18% of whom were treated with anticoagulants. For 15.71% of the NVAF patients treated with warfarin , INR met the standard during hospitalization. In the study, such factors as age, coronary heart disease, history of stroke and history of bleed-ing might affect prescription of anticoagulant drugs. Conclusion Most NVAF patients need anticoagulant thera-py, but the rate of anticoagulant therapy is lower than we expected. Among those patients treated with warfarin during hospitalization , the rate of INR meeting the standard is at a lower level. The novel oral anticoagulants are not in wide use in the clinical practice.