1.Serum Level and Significance of Specific Antibodies Against Staphyloc occus Aureus Enterotoxin B in Patients with Atopic Dermatitis and Eczema
Yan CHEN ; Fei HAO ; Qing GUO ; Guozhen TAN ; Hong JIA ; Zhigang BI ; Bo YANG ; Qingchun DIAO ; Dong YI ; Bian ZHAO
Chinese Journal of Dermatology 1994;0(06):-
0.05).Conclusions The higher level of SEB-specific IgM and IgE in AD and eczema indi cates the colonization of Staphylococcus aureus,which participates in the exace rbation of allergic inflammation,is involved in the pathogenesis of AD and ecz ema.
2.The interactions among impact factors affecting 131I treatment efficacy of Graves′ disease
Peng, WANG ; Jian, TAN ; Gui-zhi, ZHANG ; Ya-jing, HE ; Feng, DONG ; Ren-fei, WANG ; Qian, XIAO
Chinese Journal of Nuclear Medicine 2011;31(3):187-190
Objective To evaluate the possible interactions among different impact factors possibly affecting the treatment efficacy of 131I in Graves′ disease (GD). Methods Six hundred and thirty two GD patients that had been treated by 131I, with or without antithyroid drugs (ATD), were included in this study. The impact factors were pre-defined as age (x1), sex (x2), mass of thyroid (x3), course of disease (x4), initial symptom (x5), condition of disease (x6), ATD treatment duration (x7), effective half life time (x8), maximum 131I uptake rate (x9), total dose of 131I (x10), dose of 131I per gram of thyroid (x11), TRAb (x12), TSI (x13), TgAb (x14), and thyroid microsomal antibody(TMAb) level(x15). Interactions among different impact factors were studied by t-test, χ2 test and multi-variant logistic regression. Results Age, mass of thyroid, ATD treatment duration, maximum 131I uptake rate, dose of 131I per gram of thyroid tissue and TSI level were identified as independent impact factors affecting the 131I treatment efficacy on GD (χ2=6.908, t=-4.063, χ2=13.558, t=-2.553, t=4.528, χ2=9.716, all P<0.05) by uni-variant and multi-variate analyses. Loglinear and general linear model analyses showed that there existed multiple multiplicative and additive interactions among the factors of age, mass of thyroid, ATD treatment duration and maximum 131I uptake rate (likelihood χ2=8.176, P>0.05; F=2.928, 1.992, 2.629, 2.215, all P<0.05), which indicated that the treatment efficacy with co-existing multiple factors was not equal to simple summation of single factors. Conclusions The interactions among multiple factors can cause indi-rect effect on 131I treatment, which might guide the prescription of 131I dosage for GD treatment.
3.Studies on new hydrolysate of aconitine using HPLC-MS(n) and quantum chemistry calculation.
Peng TAN ; Yonggang LIU ; Jun GUAN ; Fei LI ; Ling DONG ; Yanjiang QIAO
China Journal of Chinese Materia Medica 2011;36(15):2099-2101
OBJECTIVETo study the new hydrolysate of aconitine using HPLC-MS(n) and quantum chemistry calculation.
METHODThe HPLC method was applied in gradient elution program and the mass spectrometry was in positive ion mode. Geometries of the possible hydrolysates were optimized at DFT/6-31G(d) level.
RESULTThe new hydrolysate was found and its protonated molecularions was at m/z 482. The quantum chemistry calculation results show that the product of elimination reations at C8 and C15 got the lowest energy conformation. The compound at m/z 482 was decluced to be the hydrolysate of carbony at C15.
CONCLUSIONDelydration aconine was detected for the first time and the new hydrolysis pathways of aconitine in water were deduced.
Aconitine ; chemistry ; Chromatography, High Pressure Liquid ; Hydrolysis ; Mass Spectrometry ; Models, Molecular ; Molecular Conformation
4.An experimental study(I) on the inhibition of prostatic hyperplasia with extract of seeds of Brassica alba.
Guo-Xin WU ; Yue-Xin LIN ; Min-Rui OU ; Dong-Fei TAN
China Journal of Chinese Materia Medica 2002;27(10):766-768
OBJECTIVETo study the effective fraction of the extract of seeds of Brassica alba, which inhibits experimental mice prostatic hyperplasia.
METHODAn experimental model of prostatic hyperplasia of castrated male mice induced by testosterone propionate was made. Fractions I, II and III were prepared by extracting the seeds of Brassica alba successively with ether, ethanol and water under reflux. Total extract was prepared by extracting the seeds of Brassica alba with 60% ethanol under reflux. The total extract and the three fractions were used to test the activities.
RESULTTotal extract, fractions I and II could not only significantly inhibit mice prostatic hyperplasia induced by testosterone propionate and activity of serum acid phosphatase, but also decrease wet weight of preputial glands, while fraction III is inactive.
CONCLUSIONExtract from seeds of Brassica alba can significantly inhibit mice prostatic hyperplasia induced by exterior hormone, possessing an activity of anti-androgen. Fractions I and II show an equivalent activity of total extract, which indicate that these fractions contain active components of seeds of Brassica alba which can inhibit prostatic hyperplasia.
Acid Phosphatase ; blood ; Animals ; Brassica ; chemistry ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Male ; Mice ; Orchiectomy ; Organ Size ; drug effects ; Plants, Medicinal ; chemistry ; Prostate ; drug effects ; Prostatic Hyperplasia ; blood ; chemically induced ; pathology ; Seeds ; chemistry ; Testosterone
5.Fusion expression of human renal cell carcinoma-associated antigen G250/MN/CA IX in prokaryotic expression system.
Yao-dong JIANG ; Shao-bin ZHENG ; Wang-long TAN ; Shan-chao ZHAO ; Fei REN ; Bao ZHANG
Journal of Southern Medical University 2007;27(3):307-309
OBJECTIVETo achieve high expression of human renal cell carcinoma-associated antigen G250 in Escherichia coli.
METHODSThe gene fragments encoding the protein obtained by PCR was cloned into prokaryotic expression vector pET32a(+) and expressed in E. coli Rosseta. The immunogenicity of the recombinant protein was evaluated by Western blotting.
RESULTSThe plasmid pET32a(+)/G250 was constructed and expressed in E. coli Rosseta successfully. Western blot analysis showed that the recombinant protein could be specifically recognized by monoclonal antibody M75.
CONCLUSIONEfficient G250 expression is achieved in prokaryotic expression system, which may facilitate further functional study of the protein and its monoclonal antibody preparation.
Antibodies, Monoclonal ; immunology ; Antibody Specificity ; immunology ; Antigens, Neoplasm ; genetics ; immunology ; metabolism ; Biomarkers, Tumor ; genetics ; immunology ; metabolism ; Blotting, Western ; Carbonic Anhydrase IX ; Carbonic Anhydrases ; genetics ; immunology ; metabolism ; Cloning, Molecular ; Escherichia coli ; genetics ; Gene Expression ; Humans ; Recombinant Fusion Proteins ; genetics ; immunology ; metabolism
6.An experimental study(II) on the inhibition of prostatic hyperplasia by extract of seeds of Brassica alba.
Guo-xin WU ; Yue-xin LIN ; Min-rui OU ; Dong-fei TAN
China Journal of Chinese Materia Medica 2003;28(7):643-646
OBJECTIVETo study the active components and their functionary mechanism of the extract of Brassica alba seeds, which inhibits experimental mice prostatic hyperplasia.
METHODProstatic hyperplasia of castrated male mice induced by testosterone propionate, the penetrability of capillary vessel of mice skin induced by histamine and the endermic flesh bud of rat induced by filter paper were used as experimental models. Sinalbin and beta-sitosterol separated from seeds of Brassica alba were used to test the activities.
RESULTSinalbin and beta-sitosterol(16.0 mg.kg-1.d-1 and 8.0 mg.kg-1.d-1) could significantly inhibit mice prostatic hyperplasia induced by testosterone propionate and activity of serum acid phosphatase(P < 0.01 or P < 0.05), Sinalbin(16.0 mg.kg-1.d-1)could significantly inhibit the hyperplasia of endermic flesh bud in rat induced by filter paper(P < 0.05), beta-sitosterol(16.0 mg.kg-1.d-1 and 8.0 mg.kg-1.d-1) could significantly decrease the penetrability of capillary vessel of mice skin induced by histamine.
CONCLUSIONSinalbin and beta-sitosterol have anti-androgen and anti-inflammation activities.
Androgen Antagonists ; pharmacology ; Animals ; Anti-Inflammatory Agents, Non-Steroidal ; pharmacology ; Capillary Permeability ; drug effects ; Choline ; analogs & derivatives ; isolation & purification ; pharmacology ; Female ; Male ; Mice ; Mustard Plant ; chemistry ; Orchiectomy ; Plants, Medicinal ; chemistry ; Prostatic Hyperplasia ; blood ; chemically induced ; physiopathology ; Rats ; Rats, Sprague-Dawley ; Seeds ; chemistry ; Sitosterols ; isolation & purification ; pharmacology ; Testosterone Propionate
7.Efficacy of intravitreal injection of Ranibizumab combined with 577 nm laser in the treatment of macular edema associated with retinal vein occlusion
Wang XUE-FEI ; Tan DONG-JU ; Li MING-ZHE ; Liu HUI ; Shao ZHENG-RONG ; Xiya XIAMU
International Eye Science 2017;17(12):2325-2327
·AIM: To investigate the clinical efficacy of intravitreal injection of Ranibizumab and 577nm laser in the treatment of retinal vein occlusion combined with macular edema.·METHODS: Totally 64 patients ( 64 eyes ) with retinal vein occlusion accompanied by macular edema were treated in our hospital from June 2014 to March 2017. Among them, 40 cases ( 40 eyes ) were in the central retinal vein occlusion group, 24 cases (24 eyes) were in the branch retinal vein occlusion group. They were treated with intravitreal injection of ranibizumab 0. 5mg, and the laser photocoagulation of 577nm was performed at 5 to 7d after injection. Meanwhile, 42 patients who did not wish to be treated with injection were treated with laser treatment only. The changes of the indexes before and after treatment were compared.·RESULTS: The average number of blocking group repeated injection of branch retinal vein for 1. 71 ± 0. 79, while the average number of patients with repeated injection of central retinal vein occlusion was 2. 11 ± 0. 80. All patients requiring repeated injections interval was greater than 30d. At 1mo after treatment, there was no patients with decreased visual acuity in branch retinal vein occlusion group, while there were 6 eyes with that in central retinal vein occlusion group, 14 eyes in simply laser group. The mean best corrected visual acuity (LogMAR) of the three groups was 0. 87±0. 60, 0. 57±0. 48 and 0. 54±0. 32, respectively, were significantly lower than that before treatment (1.26±0.53, 0.86±0.39, 0.76±0.26;P< 0. 05 ). The mean macular retinal thickness before treatment was 683.24±211.83, 557.39±128.29 and 545.82± 129. 76μm, were significantly higher than those at the last follow-up 412. 09±257. 38, 356. 29 ± 133. 02 and 322. 78 ± 109. 55μm ( P < 0. 05 ). There were 6 cases of subconjunctival hemorrhage in patients treated with laser therapy combined with laser therapy. The intraocular pressure increased to 25mmHg in 2 eyes in 2 patients and recovered after symptomatic treatment.· CONCLUSION: Intravitreal injection of ranibizumab combined with 577nm laser treatment can greatly enhance the visual acuity, effective decrease macular retinal thickness in patients with retinal vein occlusion and macular edema.
8.Effect of ERK on 17beta-estradiol-induced inhibition of VSMC proliferation in rats after vascular injury.
Ting-Huai WANG ; Zhi TAN ; Xiao-Dong FU ; Dan YANG ; Fei-Xue HU ; Yong-Yong LI
Acta Physiologica Sinica 2003;55(4):411-416
The aim of the present study was to investigate the effect of ERK on 17beta-estradiol (E(2)) inhibition of vascular smooth muscle cell (VSMC) proliferation in rats after vascular injury. Common carotid artery balloon-injury (Inj) model was established in ovariectomized rats (OVX). Female SD rats were randomly divided into 4 groups: OVX, E(2)+OVX, OVX+Inj, and E(2)+OVX+Inj groups. The thickness of the vessels, the plasma content of NO, and the expression of ERK, phosphorylated ERK as well as eNOS protein were measured. The results showed that compared with OVX, the vessel wall was significantly thickened and the plasma content of NO was significantly decreased in OVX+Inj group. E(2) significantly decreased the vessel thickness but increased the plasma NO content after balloon injury. E(2) inhibited the expression of ERK, phosphorylated ERK and induced the eNOS expression. There is a positive correlation between plasma NO content and eNOS protein expression, while there is a negative correlation between plasma NO content and the thickness of vessel. The plasma NO content and the expression of ERK protein were negatively correlated. These results suggest that E(2) increases the vascular eNOS protein expression and NO release, leading to the inhibition of VSMC proliferation after balloon injury by inhibiting the ERK and phosphorylated ERK protein expression.
Animals
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Carotid Artery, Common
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pathology
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Catheterization
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adverse effects
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Cell Proliferation
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drug effects
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Estradiol
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pharmacology
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Extracellular Signal-Regulated MAP Kinases
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physiology
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Female
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Muscle, Smooth, Vascular
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cytology
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Myocytes, Smooth Muscle
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cytology
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drug effects
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physiology
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Nitric Oxide
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blood
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Nitric Oxide Synthase Type III
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metabolism
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Ovariectomy
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Phosphorylation
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Rats
9.Efficacy of low-dose tadalafil on ED assessed by Self-Esteem and Relationship Questionnaire.
Jing-Ping LI ; Fei LI ; Wen-Bin GUO ; Qi-Zhao ZHOU ; Cun-Dong LIU ; Xiang-Ming MAO ; Wan-Long TAN ; Shao-Bin ZHENG
National Journal of Andrology 2010;16(12):1147-1149
OBJECTIVETo explore the effects of low-dose oral tadalafil on self-esteem, confidence and sexual relationship in ED patients.
METHODSWe treated 17 ED patients with oral tadalafil at the low dose of 5 mg once daily for 12 weeks, and used the paired t test to compare their scores on The Self-Esteem and Relationship Questionnaire (SEAR) and IIEF-5 and the results of nocturnal penile tumescence (NPT) obtained by nocturnal electrobioimpedance volumetric assessment (NEVA) before and after the medication.
RESULTSThe scores on SEAR and IIEF-5 were significantly increased (P < 0.01) and NPT markedly improved (P < 0.05) after tadalafil treatment as compared with the baseline.
CONCLUSIONLow-dose oral tadalafil once daily can significantly improve the self-esteem, confidence, sexual relationship satisfaction and NPT of ED patients.
Adult ; Carbolines ; administration & dosage ; therapeutic use ; Erectile Dysfunction ; drug therapy ; psychology ; Humans ; Male ; Middle Aged ; Self Concept ; Surveys and Questionnaires ; Tadalafil ; Vasodilator Agents ; administration & dosage ; therapeutic use
10.Correlation of aging with psychological and organic ED: nocturnal electrobioimpedance volumetric assessment of 83 cases.
Wen-Bin GUO ; Ya-Guang ZOU ; Fei LI ; Qi-Zhao ZHOU ; Tie-Qiun LI ; Xiao-Wei JING ; Shao-Bin ZHENG ; Wan-Long TAN ; Cun-Dong LIU ; Xiang-Ming MAO
National Journal of Andrology 2010;16(10):915-918
OBJECTIVEThe ratio of psychological to organic ED changes with aging. This study aimed to analyze the results of nocturnal electrobioimpedance volumetric assessment (NEVA) for ED patients of different age groups and their significance in the diagnosis of ED.
METHODSA total of 83 ED patients were divided into 4 age groups (< or = 29 yr, 30 -39 yr, 40 -49 yr and > or = 50 yr) and detected for nocturnal penile tumescence (NPT) by NEVA.
RESULTSThirty-four of the cases were diagnosed as organic ED, and the other 49 as psychological ED. With the increase of age, the former was increased from 30.3% in the < or = 29 yr group to 60.0% in the > or = 50 yr group, while the latter decreased from 69.7% to 40.0%.
CONCLUSIONThe percentage of organic ED tends to grow with the increase of age, while that of psychological ED is just the opposite.
Adult ; Aging ; Electric Impedance ; Erectile Dysfunction ; diagnosis ; physiopathology ; Humans ; Male ; Middle Aged ; Penile Erection